(Circular) (Six-base) MAPSORT of: pFN21AB6672.seq Check: 5941 from: 1 to: 6067 pFN21AB6672 6067 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3410 1937 Size: 1473 4594 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2307 4130 5514 5782 1632 Size: 675 1823 1384 268 1917 Fragments arranged by size: 1917 1823 1384 675 268 AflII C'TTAA_G Cuts at: 820 1017 2027 820 Size: 197 1010 4860 Fragments arranged by size: 4860 1010 197 AflIII A'CryG_T Cuts at: 1203 4966 5943 1203 Size: 3763 977 1327 Fragments arranged by size: 3763 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 2591 3217 3810 5382 2591 Size: 626 593 1572 3276 Fragments arranged by size: 3276 1572 626 593 ApaLI G'TGCA_C Cuts at: 4124 5280 4124 Size: 1156 4911 ApoI r'AATT_y Cuts at: 1493 2166 2540 2562 2594 3376 3602 3754 5863 Size: 673 374 22 32 782 226 152 2109 Cuts at: 5863 1493 Size: 1697 Fragments arranged by size: 2109 1697 782 673 374 226 152 32 22 AseI AT'TA_AT Cuts at: 160 1032 2787 4621 160 Size: 872 1755 1834 1606 Fragments arranged by size: 1834 1755 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 3392 1870 Size: 73 1449 4545 Fragments arranged by size: 4545 1449 73 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3388 4843 618 Size: 325 217 627 1601 1455 1842 Fragments arranged by size: 1842 1601 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3386 1391 Size: 1995 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2134 2881 928 Size: 971 235 747 4114 Fragments arranged by size: 4114 971 747 235 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4258 4292 1903 Size: 34 50 34 2237 34 3678 Fragments arranged by size: 3678 2237 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2342 2382 3125 3959 5169 2342 Size: 40 743 834 1210 3240 Fragments arranged by size: 3240 1210 834 743 40 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4678 136 Size: 107 122 71 631 605 3006 1525 Fragments arranged by size: 3006 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2695 6062 2695 Size: 3367 2700 BlpI GC'TnA_GC Cuts at: 3484 3484 Size: 6067 Bme1580I G_kGCm'C Cuts at: 1790 4128 5284 1790 Size: 2338 1156 2573 Fragments arranged by size: 2573 2338 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3798 4756 442 Size: 416 53 83 399 24 2381 958 1753 Fragments arranged by size: 2381 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2442 2442 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2071 2095 2995 4727 1239 Size: 832 24 900 1732 2579 Fragments arranged by size: 2579 1732 900 832 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4192 5057 5355 4192 Size: 865 298 4904 Fragments arranged by size: 4904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2321 4730 882 Size: 617 822 2409 2219 Fragments arranged by size: 2409 2219 822 617 BsaAI yAC'GTr Cuts at: 493 1204 3244 3858 493 Size: 711 2040 614 2702 Fragments arranged by size: 2702 2040 711 614 BsaBI GATnn'nnATC Cuts at: 2694 6061 2694 Size: 3367 2700 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3874 4256 4891 275 Size: 53 83 186 936 2341 382 635 1451 Fragments arranged by size: 2341 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2598 2613 4500 4898 5172 5319 5730 2598 Size: 15 1887 398 274 147 411 2935 Fragments arranged by size: 2935 1887 411 398 274 147 15 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2565 2595 1178 Size: 30 165 30 1162 30 4650 Fragments arranged by size: 4650 1162 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2394 2415 2998 1186 Size: 1208 21 583 4255 Fragments arranged by size: 4255 1208 583 21 BseYI C'CCAG_C Cuts at: 2058 2353 5270 5766 2058 Size: 295 2917 496 2359 Fragments arranged by size: 2917 2359 496 295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2217 2841 1163 Size: 573 481 624 4389 Fragments arranged by size: 4389 624 573 481 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3265 4128 4213 5284 3265 Size: 863 85 1071 4048 Fragments arranged by size: 4048 1071 863 85 BsmI GAATG_Cn' Cuts at: 3193 3578 3671 3193 Size: 385 93 5589 Fragments arranged by size: 5589 385 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3265 3386 4128 4213 5284 1391 Size: 399 1475 121 742 85 1071 2174 Fragments arranged by size: 2174 1475 1071 742 399 121 85 BspEI T'CCGG_A Cuts at: 2598 5730 2598 Size: 3132 2935 BspHI T'CATG_A Cuts at: 2475 3956 5686 2475 Size: 1481 1730 2856 Fragments arranged by size: 2856 1730 1481 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3422 844 Size: 2578 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3954 1394 Size: 429 2131 3507 Fragments arranged by size: 3507 2131 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4711 4898 1700 Size: 110 2901 187 2869 Fragments arranged by size: 2901 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6067 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6067 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6067 BstYI r'GATC_y Cuts at: 1972 2251 2695 3094 3397 3764 4149 4166 5618 Size: 279 444 399 303 367 385 17 1452 Cuts at: 5618 5704 5716 6062 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 444 399 385 367 346 303 279 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5515 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 2586 3583 4377 4397 2586 Size: 997 794 20 4256 Fragments arranged by size: 4256 997 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032 Size: 54 1425 50 275 18 1684 889 1629 Cuts at: 6032 8 Size: 43 Fragments arranged by size: 1684 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2283 2319 3997 1353 Size: 930 36 1678 3423 Fragments arranged by size: 3423 1678 930 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4654 5028 5174 387 Size: 1064 138 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2095 2307 2995 4130 4727 5514 Size: 393 439 24 212 688 1135 597 787 Cuts at: 5514 5782 1239 Size: 268 1524 Fragments arranged by size: 1524 1135 787 688 597 439 393 268 212 24 EcoO109I rG'GnC_Cy Cuts at: 2882 2882 Size: 6067 EcoRI G'AATT_C Cuts at: 2594 3376 3754 2594 Size: 782 378 4907 Fragments arranged by size: 4907 782 378 EcoRV GAT'ATC Cuts at: 2758 2758 Size: 6067 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 5214 1678 Size: 3536 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2389 2421 2758 2790 2829 Size: 32 135 32 817 32 337 32 39 Cuts at: 2829 2861 3293 3325 4708 4740 4782 4814 1373 Size: 32 432 32 1383 32 42 32 2626 Fragments arranged by size: 2626 1383 817 432 337 135 42 39 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2488 3411 3657 669 Size: 988 281 550 923 246 3079 Fragments arranged by size: 3079 988 923 550 281 246 HindIII A'AGCT_T Cuts at: 748 2727 748 Size: 1979 4088 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 3666 3666 Size: 6067 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 3007 3290 5181 5365 1305 Size: 969 733 283 1891 184 2007 Fragments arranged by size: 2007 1891 969 733 283 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4245 Fragments arranged by size: 4245 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2474 3186 4025 4384 4543 518 Size: 648 1308 712 839 359 159 2042 Fragments arranged by size: 2042 1308 839 712 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2591 3483 4160 5308 5553 1606 Size: 985 892 677 1148 245 2120 Fragments arranged by size: 2120 1148 985 892 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5515 NdeI CA'TA_TG Cuts at: 387 3183 387 Size: 2796 3271 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 3425 4970 1132 Size: 97 2196 1545 2229 Fragments arranged by size: 2229 2196 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6067 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6067 PfoI T'CCnGG_A Cuts at: 3090 3090 Size: 6067 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2882 2882 Size: 6067 PshAI GACnn'nnGTC Cuts at: 1613 2887 1613 Size: 1274 4793 PsiI TTA'TAA Cuts at: 3036 3637 3036 Size: 601 5466 PstI C_TGCA'G Cuts at: 830 3419 830 Size: 2589 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2591 2591 Size: 6067 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 2300 4315 6027 2300 Size: 2015 1712 2340 Fragments arranged by size: 2340 2015 1712 SfcI C'TryA_G Cuts at: 826 1045 3204 3415 4550 5231 5422 826 Size: 219 2159 211 1135 681 191 1471 Fragments arranged by size: 2159 1471 1135 681 219 211 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2027 4171 5072 5334 820 Size: 197 926 84 2144 901 262 1553 Fragments arranged by size: 2144 1553 926 901 262 197 84 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6067 StyI C'CwwG_G Cuts at: 513 1065 2316 3506 513 Size: 552 1251 1190 3074 Fragments arranged by size: 3074 1251 1190 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2429 2770 4094 4111 4264 4449 4894 Size: 1255 849 341 1324 17 153 185 445 Cuts at: 4894 325 Size: 1498 Fragments arranged by size: 1498 1324 1255 849 445 341 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 3294 4313 Size: 274 80 33 51 1443 321 996 1019 Cuts at: 4313 6025 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1019 996 321 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2404 2436 2565 2597 6027 6059 2404 Size: 32 129 32 3430 32 2412 Fragments arranged by size: 3430 2412 129 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2471 1316 Size: 1155 4912 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2567 2567 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6067 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 4196 4196 Size: 6067 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI