(Circular) (Six-base) MAPSORT of: pFN21AB6643.seq Check: 1387 from: 1 to: 5866 pFN21AB6643 5866 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2181 3209 1937 Size: 244 1028 4594 Fragments arranged by size: 4594 1028 244 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2304 3929 5313 5581 1632 Size: 672 1625 1384 268 1917 Fragments arranged by size: 1917 1625 1384 672 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 4765 5742 1203 Size: 3562 977 1327 Fragments arranged by size: 3562 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 2547 4595 2547 Size: 2048 3818 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5866 AlwNI CAG_nnn'CTG Cuts at: 3609 5181 3609 Size: 1572 4294 ApaI G_GGCC'C Cuts at: 2783 2783 Size: 5866 ApaLI G'TGCA_C Cuts at: 3923 5079 3923 Size: 1156 4710 ApoI r'AATT_y Cuts at: 1493 2887 3175 3401 3553 5662 1493 Size: 1394 288 226 152 2109 1697 Fragments arranged by size: 2109 1697 1394 288 226 152 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 2026 3191 1870 Size: 73 83 1165 4545 Fragments arranged by size: 4545 1165 83 73 BamHI G'GATC_C Cuts at: 2477 3196 3563 2477 Size: 719 367 4780 Fragments arranged by size: 4780 719 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3187 4642 618 Size: 325 217 627 1400 1455 1842 Fragments arranged by size: 1842 1455 1400 627 325 217 BanII G_rGCy'C Cuts at: 1391 2783 3185 1391 Size: 1392 402 4072 Fragments arranged by size: 4072 1392 402 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2814 928 Size: 971 915 3980 Fragments arranged by size: 3980 971 915 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3048 3758 4968 3048 Size: 710 1210 3946 Fragments arranged by size: 3946 1210 710 BclI T'GATC_A Cuts at: 1548 1548 Size: 5866 BfrBI ATG'CAT Cuts at: 2623 2915 2623 Size: 292 5574 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4477 136 Size: 107 122 71 631 605 2805 1525 Fragments arranged by size: 2805 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 3283 3283 Size: 5866 Bme1580I G_kGCm'C Cuts at: 1790 2783 3927 5083 1790 Size: 993 1144 1156 2573 Fragments arranged by size: 2573 1156 1144 993 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3597 4555 442 Size: 416 53 83 399 24 2180 958 1753 Fragments arranged by size: 2180 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2304 2304 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2286 2835 4526 1239 Size: 1047 549 1691 2579 Fragments arranged by size: 2579 1691 1047 549 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3991 4856 5154 3991 Size: 865 298 4703 Fragments arranged by size: 4703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4529 882 Size: 617 3030 2219 Fragments arranged by size: 3030 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 2205 5860 2205 Size: 3655 2211 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3673 4055 4690 275 Size: 53 83 186 936 2140 382 635 1451 Fragments arranged by size: 2140 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4299 4697 4971 5118 5529 4299 Size: 398 274 147 411 4636 Fragments arranged by size: 4636 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5641 Fragments arranged by size: 5641 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2760 1186 Size: 1574 4292 BseYI C'CCAG_C Cuts at: 2952 3114 5069 5565 2952 Size: 162 1955 496 3253 Fragments arranged by size: 3253 1955 496 162 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2334 1163 Size: 573 598 4695 Fragments arranged by size: 4695 598 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3927 4012 5083 3927 Size: 85 1071 4710 Fragments arranged by size: 4710 1071 85 BsmI GAATG_Cn' Cuts at: 3377 3470 3377 Size: 93 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2783 3185 3927 4012 5083 1391 Size: 399 993 402 742 85 1071 2174 Fragments arranged by size: 2174 1071 993 742 402 399 85 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 3755 5485 3755 Size: 1730 4136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3154 3221 844 Size: 2310 67 3489 Fragments arranged by size: 3489 2310 67 BsrBI CCG'CTC Cuts at: 1394 1823 2283 3753 1394 Size: 429 460 1470 3507 Fragments arranged by size: 3507 1470 460 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4361 4535 65 Size: 1183 3113 174 1396 Fragments arranged by size: 3113 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4510 4697 1700 Size: 110 2700 187 2869 Fragments arranged by size: 2869 2700 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5866 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 3926 4938 3926 Size: 1012 4854 BstAPI GCAn_nnn'nTGC Cuts at: 794 2164 794 Size: 1370 4496 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 2981 1184 Size: 1797 4069 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2387 2011 Size: 376 5490 BstYI r'GATC_y Cuts at: 1972 2477 3196 3563 3948 3965 5417 5503 5515 Size: 505 719 367 385 17 1452 86 12 Cuts at: 5515 5861 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 719 505 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2182 2182 Size: 5866 BtgI C'CryG_G Cuts at: 513 1065 2473 2481 513 Size: 552 1408 8 3898 Fragments arranged by size: 3898 1408 552 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 3382 4176 4196 3382 Size: 794 20 5052 Fragments arranged by size: 5052 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DraIII CAC_nnn'GTG Cuts at: 3020 3020 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4873 809 Size: 507 3557 1802 Fragments arranged by size: 3557 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2377 2899 3313 Size: 54 1425 50 275 18 547 522 414 Cuts at: 3313 4202 5831 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 547 522 414 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3796 1353 Size: 2443 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2682 4453 4827 4973 387 Size: 1064 138 1093 1771 374 146 1280 Fragments arranged by size: 1771 1280 1093 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2304 2835 3929 4526 5313 5581 Size: 393 654 18 531 1094 597 787 268 Cuts at: 5581 1239 Size: 1524 Fragments arranged by size: 1524 1094 787 654 597 531 393 268 18 EcoNI CCTnn'n_nnAGG Cuts at: 2853 2853 Size: 5866 EcoO109I rG'GnC_Cy Cuts at: 2261 2342 2414 2779 2846 3029 2261 Size: 81 72 365 67 183 5098 Fragments arranged by size: 5098 365 183 81 72 67 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 EcoRV GAT'ATC Cuts at: 2945 2945 Size: 5866 FspI TGC'GCA Cuts at: 4372 4372 Size: 5866 HaeII r_GCGC'y Cuts at: 1678 5013 1678 Size: 3335 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2533 2565 4507 4539 4581 Size: 32 135 32 961 32 1942 32 42 Cuts at: 4581 4613 1373 Size: 32 2626 Fragments arranged by size: 2626 1942 961 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2719 3210 3456 669 Size: 988 281 781 491 246 3079 Fragments arranged by size: 3079 988 781 491 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 2719 3456 2719 Size: 737 5129 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 4980 5164 1305 Size: 753 2922 184 2007 Fragments arranged by size: 2922 2007 753 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2901 10 Size: 54 1425 343 1069 2975 Fragments arranged by size: 2975 1425 1069 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2456 3824 4183 4342 518 Size: 648 843 447 1368 359 159 2042 Fragments arranged by size: 2042 1368 843 648 447 359 159 MspA1I CmG'CkG Cuts at: 1606 2060 3282 3959 5107 5352 1606 Size: 454 1222 677 1148 245 2120 Fragments arranged by size: 2120 1222 1148 677 454 245 NcoI C'CATG_G Cuts at: 513 1065 2473 2481 513 Size: 552 1408 8 3898 Fragments arranged by size: 3898 1408 552 8 NdeI CA'TA_TG Cuts at: 387 2400 2733 387 Size: 2013 333 3520 Fragments arranged by size: 3520 2013 333 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NsiI A_TGCA'T Cuts at: 2625 2917 2625 Size: 292 5574 NspI r_CATG'y Cuts at: 1132 1229 2317 2362 2524 3224 4769 1132 Size: 97 1088 45 162 700 1545 2229 Fragments arranged by size: 2229 1545 1088 700 162 97 45 PasI CC'CwG_GG Cuts at: 1348 2969 1348 Size: 1621 4245 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5866 PfoI T'CCnGG_A Cuts at: 2263 2263 Size: 5866 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2261 2342 2414 2846 3029 2261 Size: 81 72 432 183 5098 Fragments arranged by size: 5098 432 183 81 72 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PspOMI G'GGCC_C Cuts at: 2779 2779 Size: 5866 PstI C_TGCA'G Cuts at: 830 3218 830 Size: 2388 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 2795 4114 5826 2795 Size: 1319 1712 2835 Fragments arranged by size: 2835 1712 1319 SfcI C'TryA_G Cuts at: 826 1045 2761 3214 4349 5030 5221 826 Size: 219 1716 453 1135 681 191 1471 Fragments arranged by size: 1716 1471 1135 681 453 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 3193 1872 Size: 1321 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3970 4871 5133 820 Size: 197 926 2027 901 262 1553 Fragments arranged by size: 2027 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 2524 3224 1132 Size: 1392 700 3774 Fragments arranged by size: 3774 1392 700 SspI AAT'ATT Cuts at: 5 52 2873 3790 5 Size: 47 2821 917 2081 Fragments arranged by size: 2821 2081 917 47 StuI AGG'CCT Cuts at: 1517 3124 1517 Size: 1607 4259 StyI C'CwwG_G Cuts at: 513 1065 2473 2481 2608 2680 2722 3305 513 Size: 552 1408 8 127 72 42 583 3074 Fragments arranged by size: 3074 1408 583 552 127 72 42 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2254 3893 3910 4063 4248 4693 325 Size: 1255 674 1639 17 153 185 445 1498 Fragments arranged by size: 1639 1498 1255 674 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2527 2793 4112 Size: 274 80 33 51 1443 550 266 1319 Cuts at: 4112 5824 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1319 550 274 266 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5826 5858 5826 Size: 32 5834 Tth111I GACn'n_nGTC Cuts at: 1316 2844 3060 1316 Size: 1528 216 4122 Fragments arranged by size: 4122 1528 216 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2831 3146 2831 Size: 315 5551 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5866 XmaI C'CCGG_G Cuts at: 1870 3191 1870 Size: 1321 4545 XmnI GAAnn'nnTTC Cuts at: 3995 3995 Size: 5866 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI