(Circular) (Six-base) MAPSORT of: pFN21AB6631.seq Check: 8274 from: 1 to: 5917 pFN21AB6631 5917 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3260 1937 Size: 1323 4594 Acc65I G'GTAC_C Cuts at: 3238 3238 Size: 5917 AclI AA'CG_TT Cuts at: 1563 3054 4044 4417 1563 Size: 1491 990 373 3063 Fragments arranged by size: 3063 1491 990 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 2414 2483 2552 2621 2690 2759 3980 Size: 713 69 69 69 69 69 69 1221 Cuts at: 3980 5364 5632 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1221 713 268 69 69 69 69 69 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 4816 5793 1203 Size: 3613 977 1327 Fragments arranged by size: 3613 1327 977 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3169 1184 Size: 1985 3932 AlwNI CAG_nnn'CTG Cuts at: 2038 2245 2314 2383 2452 2521 2590 2659 2728 Size: 207 69 69 69 69 69 69 69 Cuts at: 2728 3191 3660 5232 2038 Size: 463 469 1572 2723 Fragments arranged by size: 2723 1572 469 463 207 69 69 69 69 69 69 69 ApaI G_GGCC'C Cuts at: 2872 2872 Size: 5917 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 3077 3122 3226 3452 3604 5713 1493 Size: 1584 45 104 226 152 2109 1697 Fragments arranged by size: 2109 1697 1584 226 152 104 45 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 2088 3242 1870 Size: 73 145 1154 4545 Fragments arranged by size: 4545 1154 145 73 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3238 4693 618 Size: 325 217 627 1451 1455 1842 Fragments arranged by size: 1842 1455 1451 627 325 217 BanII G_rGCy'C Cuts at: 1391 2172 2277 2872 3048 3236 1391 Size: 781 105 595 176 188 4072 Fragments arranged by size: 4072 781 595 188 176 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2916 3072 928 Size: 971 1017 156 3773 Fragments arranged by size: 3773 1017 971 156 BbvCI CC'TCA_GC Cuts at: 2895 2895 Size: 5917 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2087 3809 5019 2087 Size: 1722 1210 2985 Fragments arranged by size: 2985 1722 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5917 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4528 136 Size: 107 122 71 631 605 2856 1525 Fragments arranged by size: 2856 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2301 2370 2439 2508 2577 2646 2715 2962 5912 Size: 69 69 69 69 69 69 247 2950 Cuts at: 5912 2301 Size: 2306 Fragments arranged by size: 2950 2306 247 69 69 69 69 69 69 BlpI GC'TnA_GC Cuts at: 2104 3334 2104 Size: 1230 4687 Bme1580I G_kGCm'C Cuts at: 1790 2872 3978 5134 1790 Size: 1082 1106 1156 2573 Fragments arranged by size: 2573 1156 1106 1082 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3648 4606 442 Size: 416 53 83 399 24 2231 958 1753 Fragments arranged by size: 2231 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 3142 3168 4577 1239 Size: 830 1073 26 1409 2579 Fragments arranged by size: 2579 1409 1073 830 26 Bpu10I CC'TnA_GC Cuts at: 2895 2895 Size: 5917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4042 4907 5205 4042 Size: 865 298 4754 Fragments arranged by size: 4754 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4580 882 Size: 617 3081 2219 Fragments arranged by size: 3081 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2982 3708 493 Size: 711 1778 726 2702 Fragments arranged by size: 2702 1778 726 711 BsaBI GATnn'nnATC Cuts at: 2967 5911 2967 Size: 2944 2973 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3724 4106 4741 275 Size: 53 83 186 936 2191 382 635 1451 Fragments arranged by size: 2191 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4350 4748 5022 5169 5580 4350 Size: 398 274 147 411 4687 Fragments arranged by size: 4687 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5692 Fragments arranged by size: 5692 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2135 2912 3054 1186 Size: 880 69 777 142 4049 Fragments arranged by size: 4049 880 777 142 69 BseYI C'CCAG_C Cuts at: 2038 2165 2195 2250 2319 2733 2823 2871 3014 Size: 127 30 55 69 414 90 48 143 Cuts at: 3014 5120 5616 2038 Size: 2106 496 2339 Fragments arranged by size: 2339 2106 496 414 143 127 90 69 55 48 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3367 4128 5156 1540 Size: 275 186 1366 761 1028 2301 Fragments arranged by size: 2301 1366 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 3978 4063 5134 2277 Size: 1701 85 1071 3060 Fragments arranged by size: 3060 1701 1071 85 BsmI GAATG_Cn' Cuts at: 3428 3521 3428 Size: 93 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2277 2872 3048 3236 3978 4063 Size: 399 382 105 595 176 188 742 85 Cuts at: 4063 5134 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 595 399 382 188 176 105 85 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3272 844 Size: 2428 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3804 1394 Size: 429 1981 3507 Fragments arranged by size: 3507 1981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2993 3094 4412 4586 65 Size: 1183 1745 101 1318 174 1396 Fragments arranged by size: 1745 1396 1318 1183 174 101 BsrFI r'CCGG_y Cuts at: 1700 1810 4561 4748 1700 Size: 110 2751 187 2869 Fragments arranged by size: 2869 2751 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5917 BstBI TT'CG_AA Cuts at: 4721 4721 Size: 5917 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5917 BstYI r'GATC_y Cuts at: 1972 2301 2370 2439 2508 2577 2646 2715 2962 Size: 329 69 69 69 69 69 69 247 Cuts at: 2962 3129 3247 3614 3999 4016 5468 5554 5566 Size: 167 118 367 385 17 1452 86 12 Cuts at: 5566 5912 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 329 247 167 118 86 69 69 69 69 69 69 17 12 BtgI C'CryG_G Cuts at: 513 1065 2122 513 Size: 552 1057 4308 Fragments arranged by size: 4308 1057 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5178 Fragments arranged by size: 5178 683 56 BtsI GCAGTG_nn' Cuts at: 2271 2340 2409 2478 2547 2616 2685 2754 3433 Size: 69 69 69 69 69 69 69 679 Cuts at: 3433 4227 4247 2271 Size: 794 20 3941 Fragments arranged by size: 3941 794 679 69 69 69 69 69 69 69 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4924 809 Size: 507 3608 1802 Fragments arranged by size: 3608 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2119 3364 4253 Size: 54 1425 50 275 18 289 1245 889 Cuts at: 4253 5882 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1245 889 289 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 1537 Size: 275 1552 4090 Fragments arranged by size: 4090 1552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3847 1353 Size: 2494 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4504 4878 5024 387 Size: 1064 138 2915 374 146 1280 Fragments arranged by size: 2915 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2275 2275 Size: 5917 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2345 2414 2483 2552 2621 2690 Size: 393 437 276 69 69 69 69 69 Cuts at: 2690 2759 3142 3168 3980 4577 5364 5632 1239 Size: 69 383 26 812 597 787 268 1524 Fragments arranged by size: 1524 812 787 597 437 393 383 276 268 69 69 69 69 69 69 26 EcoNI CCTnn'n_nnAGG Cuts at: 2048 2051 3157 2048 Size: 3 1106 4808 Fragments arranged by size: 4808 1106 3 EcoO109I rG'GnC_Cy Cuts at: 2191 2868 2191 Size: 677 5240 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 5064 1678 Size: 3386 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4558 4590 4632 4664 1373 Size: 32 135 32 2986 32 42 32 2626 Fragments arranged by size: 2986 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3261 3507 669 Size: 988 281 1323 246 3079 Fragments arranged by size: 3079 1323 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5917 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KpnI G_GTAC'C Cuts at: 3242 3242 Size: 5917 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2104 5031 5215 1305 Size: 799 2927 184 2007 Fragments arranged by size: 2927 2007 799 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2121 10 Size: 54 1425 343 289 3806 Fragments arranged by size: 3806 1425 343 289 54 MslI CAynn'nnrTG Cuts at: 518 1166 2948 3875 4234 4393 518 Size: 648 1782 927 359 159 2042 Fragments arranged by size: 2042 1782 927 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2062 2323 2737 2827 2917 3333 4010 Size: 432 24 261 414 90 90 416 677 Cuts at: 4010 5158 5403 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 432 416 414 261 245 90 90 24 NcoI C'CATG_G Cuts at: 513 1065 2122 513 Size: 552 1057 4308 Fragments arranged by size: 4308 1057 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 3275 4820 1132 Size: 97 2046 1545 2229 Fragments arranged by size: 2229 2046 1545 97 PasI CC'CwG_GG Cuts at: 1348 2775 1348 Size: 1427 4490 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 3126 1108 Size: 2018 3899 PfoI T'CCnGG_A Cuts at: 2046 2046 Size: 5917 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PmlI CAC'GTG Cuts at: 2982 2982 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2191 2191 Size: 5917 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5917 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PspOMI G'GGCC_C Cuts at: 2868 2868 Size: 5917 PspXI vC'TCGA_Gb Cuts at: 2088 2088 Size: 5917 PstI C_TGCA'G Cuts at: 830 2034 2266 2298 2335 2367 2404 2436 2473 Size: 1204 232 32 37 32 37 32 37 Cuts at: 2473 2542 2611 2680 2749 2922 3269 830 Size: 69 69 69 69 173 347 3478 Fragments arranged by size: 3478 1204 347 232 173 69 69 69 69 37 37 37 32 32 32 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 PvuII CAG'CTG Cuts at: 2038 2062 2323 2737 2917 2038 Size: 24 261 414 180 5038 Fragments arranged by size: 5038 414 261 180 24 SacI G_AGCT'C Cuts at: 2277 2277 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 4165 5877 4165 Size: 1712 4205 SfcI C'TryA_G Cuts at: 826 1045 2030 2262 2294 2331 2363 2400 2432 Size: 219 985 232 32 37 32 37 32 Cuts at: 2432 2469 2538 2607 2676 2745 2912 2918 3265 Size: 37 69 69 69 69 167 6 347 Cuts at: 3265 4400 5081 5272 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 985 681 347 232 219 191 167 69 69 69 69 37 37 37 32 32 32 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 3244 1872 Size: 1372 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2088 4021 4922 5184 820 Size: 197 926 145 1933 901 262 1553 Fragments arranged by size: 1933 1553 926 901 262 197 145 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 2070 1517 Size: 553 5364 StyI C'CwwG_G Cuts at: 513 1065 2016 2071 2122 2187 2794 3356 513 Size: 552 951 55 51 65 607 562 3074 Fragments arranged by size: 3074 951 607 562 552 65 55 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2258 2327 2396 2465 2534 2603 2672 Size: 1255 678 69 69 69 69 69 69 Cuts at: 2672 2980 3944 3961 4114 4299 4744 325 Size: 308 964 17 153 185 445 1498 Fragments arranged by size: 1498 1255 964 678 445 308 185 153 69 69 69 69 69 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4163 5875 96 Size: 274 80 33 51 1443 2186 1712 138 Fragments arranged by size: 2186 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2744 2776 2966 2998 5877 5909 2744 Size: 32 190 32 2879 32 2752 Fragments arranged by size: 2879 2752 190 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5917 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XhoI C'TCGA_G Cuts at: 1943 2088 1943 Size: 145 5772 XmaI C'CCGG_G Cuts at: 1870 3242 1870 Size: 1372 4545 XmnI GAAnn'nnTTC Cuts at: 3174 4046 3174 Size: 872 5045 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI