Restriction map for
pFN21AB6631
(Linear) (Six-base) MAPSORT of: KIBB6631.seq Check: 8396 from: 1 to: 1212
KIBB6631.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AclI AA'CG_TT
Cuts at: 0 1049 1212
Size: 1049 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 409 478 547 616 685 754 1212
Size: 340 69 69 69 69 69 69 458
Fragments arranged by size:
458 340 69 69 69 69 69 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1164 1212
Size: 1164 48
AlwNI CAG_nnn'CTG
Cuts at: 0 33 240 309 378 447 516 585 654
Size: 33 207 69 69 69 69 69 69
Cuts at: 654 723 1186 1212
Size: 69 463 26
Fragments arranged by size:
463 207 69 69 69 69 69 69
69 33 26
ApaI G_GGCC'C
Cuts at: 0 867 1212
Size: 867 345
ApoI r'AATT_y
Cuts at: 0 1072 1117 1212
Size: 1072 45 95
Fragments arranged by size:
1072 95 45
AvaI C'yCGr_G
Cuts at: 0 83 1212
Size: 83 1129
BanII G_rGCy'C
Cuts at: 0 167 272 867 1043 1212
Size: 167 105 595 176 169
Fragments arranged by size:
595 176 169 167 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 911 1067 1212
Size: 911 156 145
Fragments arranged by size:
911 156 145
BbvCI CC'TCA_GC
Cuts at: 0 890 1212
Size: 890 322
BciVI GTATCCnnnnn_n'
Cuts at: 0 82 1212
Size: 82 1130
BglII A'GATC_T
Cuts at: 0 296 365 434 503 572 641 710 957
Size: 296 69 69 69 69 69 69 247
Cuts at: 957 1212
Size: 255
Fragments arranged by size:
296 255 247 69 69 69 69 69
69
BlpI GC'TnA_GC
Cuts at: 0 99 1212
Size: 99 1113
Bme1580I G_kGCm'C
Cuts at: 0 867 1212
Size: 867 345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1137 1163 1212
Size: 64 1073 26 49
Fragments arranged by size:
1073 64 49 26
Bpu10I CC'TnA_GC
Cuts at: 0 890 1212
Size: 890 322
BsaAI yAC'GTr
Cuts at: 0 977 1212
Size: 977 235
BsaBI GATnn'nnATC
Cuts at: 0 962 1212
Size: 962 250
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 130 907 1049 1212
Size: 61 69 777 142 163
Fragments arranged by size:
777 163 142 69 61
BseYI C'CCAG_C
Cuts at: 0 33 160 190 245 314 728 818 866
Size: 33 127 30 55 69 414 90 48
Cuts at: 866 1009 1212
Size: 143 203
Fragments arranged by size:
414 203 143 127 90 69 55 48
33 30
BsiHKAI G_wGCw'C
Cuts at: 0 272 1212
Size: 272 940
Bsp1286I G_dGCh'C
Cuts at: 0 167 272 867 1043 1212
Size: 167 105 595 176 169
Fragments arranged by size:
595 176 169 167 105
BsrDI GCAATG_nn'
Cuts at: 0 988 1089 1212
Size: 988 101 123
Fragments arranged by size:
988 123 101
BstYI r'GATC_y
Cuts at: 0 296 365 434 503 572 641 710 957
Size: 296 69 69 69 69 69 69 247
Cuts at: 957 1124 1212
Size: 167 88
Fragments arranged by size:
296 247 167 88 69 69 69 69
69 69
BtgI C'CryG_G
Cuts at: 0 117 1212
Size: 117 1095
BtsI GCAGTG_nn'
Cuts at: 0 266 335 404 473 542 611 680 749
Size: 266 69 69 69 69 69 69 69
Cuts at: 749 1212
Size: 463
Fragments arranged by size:
463 266 69 69 69 69 69 69
69
EaeI y'GGCC_r
Cuts at: 0 114 1212
Size: 114 1098
EcoICRI GAG'CTC
Cuts at: 0 270 1212
Size: 270 942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 340 409 478 547 616 685 754
Size: 64 276 69 69 69 69 69 69
Cuts at: 754 1137 1163 1212
Size: 383 26 49
Fragments arranged by size:
383 276 69 69 69 69 69 69
64 49 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 43 46 1152 1212
Size: 43 3 1106 60
Fragments arranged by size:
1106 60 43 3
EcoO109I rG'GnC_Cy
Cuts at: 0 186 863 1212
Size: 186 677 349
Fragments arranged by size:
677 349 186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 1212
Size: 99 1113
MscI TGG'CCA
Cuts at: 0 116 1212
Size: 116 1096
MslI CAynn'nnrTG
Cuts at: 0 943 1212
Size: 943 269
MspA1I CmG'CkG
Cuts at: 0 33 57 318 732 822 912 1212
Size: 33 24 261 414 90 90 300
Fragments arranged by size:
414 300 261 90 90 33 24
NcoI C'CATG_G
Cuts at: 0 117 1212
Size: 117 1095
PasI CC'CwG_GG
Cuts at: 0 770 1212
Size: 770 442
PflMI CCAn_nnn'nTGG
Cuts at: 0 1121 1212
Size: 1121 91
PfoI T'CCnGG_A
Cuts at: 0 41 1212
Size: 41 1171
PmlI CAC'GTG
Cuts at: 0 977 1212
Size: 977 235
PpuMI rG'GwC_Cy
Cuts at: 0 186 1212
Size: 186 1026
PspOMI G'GGCC_C
Cuts at: 0 863 1212
Size: 863 349
PspXI vC'TCGA_Gb
Cuts at: 0 83 1212
Size: 83 1129
PstI C_TGCA'G
Cuts at: 0 29 261 293 330 362 399 431 468
Size: 29 232 32 37 32 37 32 37
Cuts at: 468 537 606 675 744 917 1212
Size: 69 69 69 69 173 295
Fragments arranged by size:
295 232 173 69 69 69 69 37
37 37 32 32 32 29
PvuII CAG'CTG
Cuts at: 0 33 57 318 732 912 1212
Size: 33 24 261 414 180 300
Fragments arranged by size:
414 300 261 180 33 24
SacI G_AGCT'C
Cuts at: 0 272 1212
Size: 272 940
SfcI C'TryA_G
Cuts at: 0 25 257 289 326 358 395 427 464
Size: 25 232 32 37 32 37 32 37
Cuts at: 464 533 602 671 740 907 913 1212
Size: 69 69 69 69 167 6 299
Fragments arranged by size:
299 232 167 69 69 69 69 37
37 37 32 32 32 25 6
SmlI C'TyrA_G
Cuts at: 0 83 1212
Size: 83 1129
StuI AGG'CCT
Cuts at: 0 65 1212
Size: 65 1147
StyI C'CwwG_G
Cuts at: 0 11 66 117 182 789 1212
Size: 11 55 51 65 607 423
Fragments arranged by size:
607 423 65 55 51 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 322 391 460 529 598 667 975
Size: 253 69 69 69 69 69 69 308
Cuts at: 975 1212
Size: 237
Fragments arranged by size:
308 253 237 69 69 69 69 69
69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 739 771 961 993 1212
Size: 739 32 190 32 219
Fragments arranged by size:
739 219 190 32 32
XhoI C'TCGA_G
Cuts at: 0 83 1212
Size: 83 1129
XmnI GAAnn'nnTTC
Cuts at: 0 1169 1212
Size: 1169 43
Enzymes that do cut:
AclI AcuI AlfI AlwNI ApaI ApoI AvaI BanII
BbsI BbvCI BciVI BglII BlpI Bme1580I BpmI Bpu10I
BsaAI BsaBI BseRI BseYI BsiHKAI Bsp1286I BsrDI BstYI
BtgI BtsI EaeI EcoICRI Eco57MI EcoNI EcoO109I MmeI
MscI MslI MspA1I NcoI PasI PflMI PfoI PmlI
PpuMI PspOMI PspXI PstI PvuII SacI SfcI SmlI
StuI StyI TaqII TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BcgI BclI BfrBI BglI
BmgBI BmrI BmtI BplI BpuEI BsaI BsaHI BsaWI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XcmI XmaI ZraI