Restriction map for
pFN21AB6618
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 677 CGCG CG^CG
AlwNI 1169 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 175 ATTAAT AT^TAAT
AsnI 175 ATTAAT AT^TAAT
Asp700I 1218 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1124 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 932 CTCGAG C^[CT]CG[GA]G
BamHI 981 GGATCC G^GATCC
BbsI 30 GAAGAC GAAGAC(2/6)
BcnI 652 CCCGG CC^[GC]GG
BpuAI 30 GAAGAC GAAGAC(2/6)
BseRI 1277 CTCCTC GAGGAG(10/8)
BsoBI 932 CTCGAG C^[CT]CG[GA]G
BspMI 492 GCAGGT ACCTGC(4/8)
BsrDI 455 CATTGC GCAATG(2/0)
BstUI 677 CGCG CG^CG
CfoI 676 GCGC GCG^C
DraII 320 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 668 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 499 GAAGAG CTCTTC(1/4)
EcoO109I 320 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 1099 GATATC GAT^ATC
FauI 678 GCGGG CCCGC(4/6)
HgaI 1160 GCGTC GACGC(5/10)
HhaI 676 GCGC GCG^C
HinP1I 676 GCGC G^CGC
Ksp632I 499 GAAGAG CTCTTC(1/4)
MslI 1121 CACGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 25 CAGCGG C[AC]G^C[GT]G
MvnI 677 CGCG CG^CG
NciI 652 CCCGG CC^[GC]GG
PaeR7I 932 CTCGAG C^TCGAG
PflFI 1124 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 320 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 175 ATTAAT AT^TAAT
PstI 242 CTGCAG CTGCA^G
SfcI 242 CTGCAG C^T[GA][CT]AG
SspI 940 AATATT AAT^ATT
Tth111I 1124 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1041 TCTAGA T^CTAGA
XhoI 932 CTCGAG C^TCGAG
XmnI 1218 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AciI 27 GCGG CCGC(-3/-1)
678 GCGG
BalI 788 TGGCCA TGG^CCA
956 TGGCCA
BanI 16 GGTGCC G^G[CT][GA]CC
387 GGCACC
BbvI 241 GCTGC GCAGC(8/12)
636 GCTGC
BclI 378 TGATCA T^GATCA
905 TGATCA
BfaI 125 CTAG C^TAG
1042 CTAG
BpmI 259 CTCCAG CTGGAG(16/14)
866 CTGGAG
BsgI 1164 CTGCAC GTGCAG(16/14)
883 GTGCAG
Bsp1407I 292 TGTACA T^GTACA
735 TGTACA
BsrGI 292 TGTACA T^GTACA
735 TGTACA
BstXI 223 CCAGCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
356 CCAAGTTTGTGG
BstYI 724 GGATCT [GA]^GATC[CT]
981 GGATCC
EaeI 788 TGGCCA [CT]^GGCC[GA]
956 TGGCCA
EcoT14I 609 CCTTGG C^C[AT][AT]GG
626 CCAAGG
FbaI 378 TGATCA T^GATCA
905 TGATCA
Fnu4HI 241 GCTGC GC^[ACGT]GC
636 GCTGC
HapII 4 CCGG C^CGG
653 CCGG
HpaII 4 CCGG C^CGG
653 CCGG
HphI 751 GGTGA GGTGA(8/7)
871 GGTGA
ItaI 241 GCTGC GC^[ACGT]GC
636 GCTGC
MaeI 125 CTAG C^TAG
1042 CTAG
MaeII 341 ACGT A^CGT
746 ACGT
MflI 724 GGATCT [GA]^GATC[CT]
981 GGATCC
MluNI 788 TGGCCA TGG^CCA
956 TGGCCA
MscI 788 TGGCCA TGG^CCA
956 TGGCCA
MspI 4 CCGG C^CGG
653 CCGG
NlaIII 486 CATG CATG^
1287 CATG
SacI 68 GAGCTC GAGCT^C
181 GAGCTC
SmlI 166 CTCAAG C^T[CT][GA]AG
932 CTCGAG
SspBI 292 TGTACA T^GTACA
735 TGTACA
StyI 609 CCTTGG C^C[AT][AT]GG
626 CCAAGG
TseI 241 GCTGC G^C[AT]GC
636 GCTGC
Tsp45I 514 GTCAC ^GT[GC]AC
872 GTGAC
XhoII 724 GGATCT [GA]^GATC[CT]
981 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BanII BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII FokI HaeIII
HinfI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I Tsp509I TspRI
TthHB8I XcmI