(Circular) (Six-base) MAPSORT of: pFN21AB6618.seq Check: 3153 from: 1 to: 6004 pFN21AB6618 6004 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2488 2488 Size: 6004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3347 1937 Size: 1410 4594 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 3303 4131 4504 1563 Size: 1740 828 373 3063 Fragments arranged by size: 3063 1740 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4067 5451 5719 1632 Size: 2435 1384 268 1917 Fragments arranged by size: 2435 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 4903 5880 1203 Size: 3700 977 1327 Fragments arranged by size: 3700 1327 977 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 3130 1166 Size: 1964 4040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AlwNI CAG_nnn'CTG Cuts at: 3179 3747 5319 3179 Size: 568 1572 3864 Fragments arranged by size: 3864 1572 568 ApaLI G'TGCA_C Cuts at: 4061 5217 4061 Size: 1156 4848 ApoI r'AATT_y Cuts at: 1493 2448 3029 3269 3313 3539 3691 5800 1493 Size: 955 581 240 44 226 152 2109 1697 Fragments arranged by size: 2109 1697 955 581 240 226 152 44 AseI AT'TA_AT Cuts at: 160 1032 2181 4558 160 Size: 872 1149 2377 1606 Fragments arranged by size: 2377 1606 1149 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 2937 3329 1870 Size: 73 994 392 4545 Fragments arranged by size: 4545 994 392 73 BamHI G'GATC_C Cuts at: 2986 3334 3701 2986 Size: 348 367 5289 Fragments arranged by size: 5289 367 348 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2392 3325 4780 618 Size: 325 217 627 234 371 933 1455 1842 Fragments arranged by size: 1842 1455 933 627 371 325 234 217 BanII G_rGCy'C Cuts at: 1391 2077 2190 2767 2854 3323 1391 Size: 686 113 577 87 469 4072 Fragments arranged by size: 4072 686 577 469 113 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2042 928 Size: 971 143 4890 Fragments arranged by size: 4890 971 143 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4195 4229 1903 Size: 34 50 34 2174 34 3678 Fragments arranged by size: 3678 2174 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 2383 2910 1548 Size: 835 527 4642 Fragments arranged by size: 4642 835 527 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4615 136 Size: 107 122 71 631 605 2943 1525 Fragments arranged by size: 2943 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5999 5999 Size: 6004 BlpI GC'TnA_GC Cuts at: 3421 3421 Size: 6004 Bme1580I G_kGCm'C Cuts at: 1790 4065 5221 1790 Size: 2275 1156 2573 Fragments arranged by size: 2573 2275 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3735 4693 442 Size: 416 53 83 399 24 2318 958 1753 Fragments arranged by size: 2318 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2892 4664 1239 Size: 1010 643 1772 2579 Fragments arranged by size: 2579 1772 1010 643 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2156 4129 4994 5292 2156 Size: 1973 865 298 2868 Fragments arranged by size: 2868 1973 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4667 882 Size: 617 3168 2219 Fragments arranged by size: 3168 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3795 493 Size: 711 2591 2702 Fragments arranged by size: 2702 2591 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4437 4835 5109 5256 5667 4437 Size: 398 274 147 411 4774 Fragments arranged by size: 4774 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5779 Fragments arranged by size: 5779 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3273 1186 Size: 2087 3917 BseYI C'CCAG_C Cuts at: 5207 5703 5207 Size: 496 5508 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2909 3154 1163 Size: 573 1173 245 4013 Fragments arranged by size: 4013 1173 573 245 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2077 2123 2190 3202 4065 4150 5221 2077 Size: 46 67 1012 863 85 1071 2860 Fragments arranged by size: 2860 1071 1012 863 85 67 46 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2123 2190 2767 2854 3202 3323 Size: 399 287 46 67 577 87 348 121 Cuts at: 3323 4065 4150 5221 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 577 399 348 287 121 87 85 67 46 BspEI T'CCGG_A Cuts at: 5667 5667 Size: 6004 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2488 3359 844 Size: 1644 871 3489 Fragments arranged by size: 3489 1644 871 BsrBI CCG'CTC Cuts at: 1394 1823 3891 1394 Size: 429 2068 3507 Fragments arranged by size: 3507 2068 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2459 4499 4673 65 Size: 1183 1211 2040 174 1396 Fragments arranged by size: 2040 1396 1211 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4648 4835 1700 Size: 110 2838 187 2869 Fragments arranged by size: 2869 2838 187 110 BsrGI T'GTAC_A Cuts at: 96 2297 2740 96 Size: 2201 443 3360 Fragments arranged by size: 3360 2201 443 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6004 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstXI CCAn_nnnn'nTGG Cuts at: 2235 2368 2235 Size: 133 5871 BstYI r'GATC_y Cuts at: 1972 2729 2986 3334 3701 4086 4103 5555 5641 Size: 757 257 348 367 385 17 1452 86 Cuts at: 5641 5653 5999 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 757 385 367 348 346 257 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5452 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 3520 4314 4334 3520 Size: 794 20 5190 Fragments arranged by size: 5190 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DraIII CAC_nnn'GTG Cuts at: 2678 2678 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2793 2961 3451 Size: 54 1425 50 275 18 963 168 490 Cuts at: 3451 4340 5969 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 963 889 490 275 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2499 3934 1353 Size: 1146 1435 3423 Fragments arranged by size: 3423 1435 1146 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2075 2188 2075 Size: 113 5891 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2892 4067 4664 5451 5719 1239 Size: 393 617 643 1175 597 787 268 1524 Fragments arranged by size: 1524 1175 787 643 617 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2326 2326 Size: 6004 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 EcoRV GAT'ATC Cuts at: 3106 3106 Size: 6004 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2831 3247 2831 Size: 416 5588 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 5151 1678 Size: 3473 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3064 3096 4645 4677 4719 Size: 32 135 32 1492 32 1549 32 42 Cuts at: 4719 4751 1373 Size: 32 2626 Fragments arranged by size: 2626 1549 1492 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2883 3035 5118 5302 1305 Size: 1578 152 2083 184 2007 Fragments arranged by size: 2083 2007 1578 184 152 MscI TGG'CCA Cuts at: 10 64 1489 1832 2795 2963 10 Size: 54 1425 343 963 168 3051 Fragments arranged by size: 3051 1425 963 343 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 3130 3962 4321 4480 518 Size: 648 1964 832 359 159 2042 Fragments arranged by size: 2042 1964 832 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2032 3420 4097 5245 5490 1606 Size: 426 1388 677 1148 245 2120 Fragments arranged by size: 2120 1388 1148 677 426 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5452 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 3362 4907 1132 Size: 97 2133 1545 2229 Fragments arranged by size: 2229 2133 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6004 PciI A'CATG_T Cuts at: 4903 4903 Size: 6004 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6004 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2326 2326 Size: 6004 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PspXI vC'TCGA_Gb Cuts at: 2937 2937 Size: 6004 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2265 2297 2265 Size: 32 5972 PstI C_TGCA'G Cuts at: 830 2251 3356 830 Size: 1421 1105 3478 Fragments arranged by size: 3478 1421 1105 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 SacI G_AGCT'C Cuts at: 2077 2190 2077 Size: 113 5891 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 4252 5964 4252 Size: 1712 4292 SfcI C'TryA_G Cuts at: 826 1045 2247 3352 4487 5168 5359 826 Size: 219 1202 1105 1135 681 191 1471 Fragments arranged by size: 1471 1202 1135 1105 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 3331 1872 Size: 1459 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2171 2937 4108 5009 5271 820 Size: 197 926 228 766 1171 901 262 1553 Fragments arranged by size: 1553 1171 926 901 766 262 228 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 2947 3928 5 Size: 47 2895 981 2081 Fragments arranged by size: 2895 2081 981 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6004 StyI C'CwwG_G Cuts at: 513 1065 2614 2631 3443 513 Size: 552 1549 17 812 3074 Fragments arranged by size: 3074 1549 812 552 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4031 4048 4201 4386 4831 325 Size: 1255 2451 17 153 185 445 1498 Fragments arranged by size: 2451 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2297 2740 4250 Size: 274 80 33 51 1443 320 443 1510 Cuts at: 4250 5962 96 Size: 1712 138 Fragments arranged by size: 1712 1510 1443 443 320 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5964 5996 5964 Size: 32 5972 Tth111I GACn'n_nGTC Cuts at: 1316 3132 1316 Size: 1816 4188 XbaI T'CTAG_A Cuts at: 3046 3340 3046 Size: 294 5710 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2074 2232 2368 2497 2074 Size: 158 136 129 5581 Fragments arranged by size: 5581 158 136 129 XhoI C'TCGA_G Cuts at: 1943 2937 1943 Size: 994 5010 XmaI C'CCGG_G Cuts at: 1870 3329 1870 Size: 1459 4545 XmnI GAAnn'nnTTC Cuts at: 3227 4133 3227 Size: 906 5098 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI