(Circular) (Six-base) MAPSORT of: pFN21AB6593.seq Check: 4537 from: 1 to: 5836 pFN21AB6593 5836 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2315 2784 2807 2993 3899 5283 5551 1632 Size: 683 469 23 186 906 1384 268 1917 Fragments arranged by size: 1917 1384 906 683 469 268 186 23 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 2478 4735 5712 1203 Size: 1275 2257 977 1327 Fragments arranged by size: 2257 1327 1275 977 AgeI A'CCGG_T Cuts at: 2501 4667 2501 Size: 2166 3670 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 2024 1166 Size: 858 4978 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2987 3019 2987 Size: 32 5804 AlwNI CAG_nnn'CTG Cuts at: 2207 2729 3579 5151 2207 Size: 522 850 1572 2892 Fragments arranged by size: 2892 1572 850 522 ApaI G_GGCC'C Cuts at: 2535 2535 Size: 5836 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 2618 3145 3371 3523 5632 1493 Size: 1125 527 226 152 2109 1697 Fragments arranged by size: 2109 1697 1125 527 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2999 3161 1870 Size: 73 1056 162 4545 Fragments arranged by size: 4545 1056 162 73 BamHI G'GATC_C Cuts at: 3040 3166 3533 3040 Size: 126 367 5343 Fragments arranged by size: 5343 367 126 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2585 2603 3157 4612 618 Size: 325 217 627 798 18 554 1455 1842 Fragments arranged by size: 1842 1455 798 627 554 325 217 18 BanII G_rGCy'C Cuts at: 1391 2535 3077 3155 1391 Size: 1144 542 78 4072 Fragments arranged by size: 4072 1144 542 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2772 3132 928 Size: 971 873 360 3632 Fragments arranged by size: 3632 971 873 360 BbvCI CC'TCA_GC Cuts at: 2736 2736 Size: 5836 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4027 4061 1903 Size: 34 2090 34 3678 Fragments arranged by size: 3678 2090 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2225 3728 4938 2225 Size: 1503 1210 3123 Fragments arranged by size: 3123 1503 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 2530 2714 Size: 107 122 71 631 605 616 242 184 Cuts at: 2714 3089 4447 136 Size: 375 1358 1525 Fragments arranged by size: 1525 1358 631 616 605 375 242 184 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 2938 3253 2938 Size: 315 5521 Bme1580I G_kGCm'C Cuts at: 1790 2535 3897 5053 1790 Size: 745 1362 1156 2573 Fragments arranged by size: 2573 1362 1156 745 BmgBI CAC'GTC Cuts at: 1318 2986 1318 Size: 1668 4168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2345 2529 2628 Size: 416 53 83 399 24 928 184 99 Cuts at: 2628 2766 2832 3567 4525 442 Size: 138 66 735 958 1753 Fragments arranged by size: 1753 958 928 735 416 399 184 138 99 83 66 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4496 1239 Size: 3257 2579 Bpu10I CC'TnA_GC Cuts at: 2646 2736 2837 2646 Size: 90 101 5645 Fragments arranged by size: 5645 101 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2381 3961 4826 5124 2381 Size: 1580 865 298 3093 Fragments arranged by size: 3093 1580 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4499 882 Size: 617 3000 2219 Fragments arranged by size: 3000 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3627 493 Size: 711 2423 2702 Fragments arranged by size: 2702 2423 711 BsaBI GATnn'nnATC Cuts at: 2327 2795 5830 2327 Size: 468 3035 2333 Fragments arranged by size: 3035 2333 468 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3643 4025 4660 275 Size: 53 83 186 936 2110 382 635 1451 Fragments arranged by size: 2110 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2501 4269 4667 4941 5088 5499 2501 Size: 1768 398 274 147 411 2838 Fragments arranged by size: 2838 1768 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2509 2539 1178 Size: 30 165 30 1106 30 4475 Fragments arranged by size: 4475 1106 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2456 2714 2717 2984 1186 Size: 1270 258 3 267 4038 Fragments arranged by size: 4038 1270 267 258 3 BseYI C'CCAG_C Cuts at: 2088 2593 5039 5535 2088 Size: 505 2446 496 2389 Fragments arranged by size: 2446 2389 505 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2248 1163 Size: 573 512 4751 Fragments arranged by size: 4751 573 512 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3897 3982 5053 3897 Size: 85 1071 4680 Fragments arranged by size: 4680 1071 85 BsmI GAATG_Cn' Cuts at: 3347 3440 3347 Size: 93 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2535 3077 3155 3897 3982 5053 1391 Size: 399 745 542 78 742 85 1071 2174 Fragments arranged by size: 2174 1071 745 742 542 399 85 78 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3191 844 Size: 2347 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2331 4331 4505 65 Size: 1183 1083 2000 174 1396 Fragments arranged by size: 2000 1396 1183 1083 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2501 4480 4667 1700 Size: 110 691 1979 187 2869 Fragments arranged by size: 2869 1979 691 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 2729 794 Size: 1935 3901 BstBI TT'CG_AA Cuts at: 3061 4640 3061 Size: 1579 4257 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5836 BstYI r'GATC_y Cuts at: 1972 2371 2629 3040 3166 3533 3918 3935 5387 Size: 399 258 411 126 367 385 17 1452 Cuts at: 5387 5473 5485 5831 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 411 399 385 367 346 258 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4345 Fragments arranged by size: 4345 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 3352 4146 4166 3352 Size: 794 20 5022 Fragments arranged by size: 5022 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 2815 2905 Size: 54 1425 50 275 18 379 606 90 Cuts at: 2905 3283 4172 5801 8 Size: 378 889 1629 43 Fragments arranged by size: 1629 1425 889 606 379 378 275 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2433 2673 2850 3766 1353 Size: 1080 240 177 916 3423 Fragments arranged by size: 3423 1080 916 240 177 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2927 4423 4797 4943 387 Size: 1064 138 1338 1496 374 146 1280 Fragments arranged by size: 1496 1338 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2784 2807 2993 3899 4496 5283 Size: 393 683 469 23 186 906 597 787 Cuts at: 5283 5551 1239 Size: 268 1524 Fragments arranged by size: 1524 906 787 683 597 469 393 268 186 23 EcoNI CCTnn'n_nnAGG Cuts at: 2399 2399 Size: 5836 EcoO109I rG'GnC_Cy Cuts at: 2995 2995 Size: 5836 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2275 2275 Size: 5836 FspI TGC'GCA Cuts at: 2327 4342 2327 Size: 2015 3821 FspAI rTGC'GCAy Cuts at: 2327 2327 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 4983 1678 Size: 3305 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2509 2541 2921 2953 4477 Size: 32 135 32 937 32 380 32 1524 Cuts at: 4477 4509 4551 4583 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1524 937 380 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2104 2419 3180 3426 669 Size: 988 281 166 315 761 246 3079 Fragments arranged by size: 3079 988 761 315 281 246 166 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1992 2263 4950 5134 1305 Size: 687 271 2687 184 2007 Fragments arranged by size: 2687 2007 687 271 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2907 10 Size: 54 1425 343 1075 2939 Fragments arranged by size: 2939 1425 1075 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 2276 2879 3794 4153 4312 518 Size: 648 858 252 603 915 359 159 2042 Fragments arranged by size: 2042 915 858 648 603 359 252 159 MspA1I CmG'CkG Cuts at: 1606 2686 2692 2720 3252 3929 5077 5322 1606 Size: 1080 6 28 532 677 1148 245 2120 Fragments arranged by size: 2120 1148 1080 677 532 245 28 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4345 Fragments arranged by size: 4345 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5836 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 2207 1108 Size: 1099 4737 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2491 2523 3905 3937 5418 5450 2491 Size: 32 1382 32 1481 32 2877 Fragments arranged by size: 2877 1481 1382 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2995 2995 Size: 5836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PspOMI G'GGCC_C Cuts at: 2531 2531 Size: 5836 PstI C_TGCA'G Cuts at: 830 2085 2725 2892 3188 830 Size: 1255 640 167 296 3478 Fragments arranged by size: 3478 1255 640 296 167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2720 2720 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SapI GCTCTTCn'nnn_ Cuts at: 2673 2850 2673 Size: 177 5659 SbfI CC_TGCA'GG Cuts at: 2892 3188 2892 Size: 296 5540 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 2081 2721 2888 3184 4319 5000 5191 Size: 219 1036 640 167 296 1135 681 191 Cuts at: 5191 826 Size: 1471 Fragments arranged by size: 1471 1135 1036 681 640 296 219 191 167 SfiI GGCCn_nnn'nGGCC Cuts at: 3089 3089 Size: 5836 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2396 3940 4841 5103 820 Size: 197 926 453 1544 901 262 1553 Fragments arranged by size: 1553 1544 926 901 453 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 2004 2244 2655 2964 3096 3275 513 Size: 552 939 240 411 309 132 179 3074 Fragments arranged by size: 3074 939 552 411 309 240 179 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3863 3880 4033 4218 4663 325 Size: 1255 2283 17 153 185 445 1498 Fragments arranged by size: 2283 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4082 5794 96 Size: 274 80 33 51 1443 2105 1712 138 Fragments arranged by size: 2105 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2337 2369 2812 2844 5796 5828 2337 Size: 32 443 32 2952 32 2345 Fragments arranged by size: 2952 2345 443 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2810 2941 2810 Size: 131 5705 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 2503 3057 3965 2503 Size: 554 908 4374 Fragments arranged by size: 4374 908 554 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI