(Circular) (Six-base) MAPSORT of: pFN21AB6120.seq Check: 2821 from: 1 to: 6454 pFN21AB6120 6454 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4264 278 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2154 3797 1937 Size: 217 1643 4594 Fragments arranged by size: 4594 1643 217 Acc65I G'GTAC_C Cuts at: 3775 3775 Size: 6454 AclI AA'CG_TT Cuts at: 1563 4581 4954 1563 Size: 3018 373 3063 Fragments arranged by size: 3063 3018 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2283 2600 3213 3507 3572 4517 5901 6169 Size: 651 317 613 294 65 945 1384 268 Cuts at: 6169 1632 Size: 1917 Fragments arranged by size: 1917 1384 945 651 613 317 294 268 65 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6257 AflIII A'CryG_T Cuts at: 1203 3544 5353 6330 1203 Size: 2341 1809 977 1327 Fragments arranged by size: 2341 1809 1327 977 AgeI A'CCGG_T Cuts at: 5285 5285 Size: 6454 AhdI GACnn_n'nnGTC Cuts at: 5183 5183 Size: 6454 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6454 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6454 AlwNI CAG_nnn'CTG Cuts at: 2366 4197 5769 2366 Size: 1831 1572 3051 Fragments arranged by size: 3051 1831 1572 ApaLI G'TGCA_C Cuts at: 4511 5667 4511 Size: 1156 5298 ApoI r'AATT_y Cuts at: 1493 2054 2252 2765 3272 3763 3989 4141 6250 Size: 561 198 513 507 491 226 152 2109 Cuts at: 6250 1493 Size: 1697 Fragments arranged by size: 2109 1697 561 513 507 491 226 198 152 AseI AT'TA_AT Cuts at: 160 1032 2282 5008 160 Size: 872 1250 2726 1606 Fragments arranged by size: 2726 1606 1250 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 AvaI C'yCGr_G Cuts at: 1870 1943 3779 1870 Size: 73 1836 4545 Fragments arranged by size: 4545 1836 73 BamHI G'GATC_C Cuts at: 3784 4151 3784 Size: 367 6087 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2794 3775 5230 618 Size: 325 217 627 1007 981 1455 1842 Fragments arranged by size: 1842 1455 1007 981 627 325 217 BanII G_rGCy'C Cuts at: 1391 3773 1391 Size: 2382 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3372 928 Size: 971 1473 4010 Fragments arranged by size: 4010 1473 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4645 4679 1903 Size: 34 50 34 2624 34 3678 Fragments arranged by size: 3678 2624 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4346 5556 4346 Size: 1210 5244 BclI T'GATC_A Cuts at: 1548 1548 Size: 6454 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5065 136 Size: 107 122 71 631 605 3393 1525 Fragments arranged by size: 3393 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2593 6449 2593 Size: 3856 2598 BlpI GC'TnA_GC Cuts at: 2046 3871 2046 Size: 1825 4629 Bme1580I G_kGCm'C Cuts at: 1790 2968 4515 5671 1790 Size: 1178 1547 1156 2573 Fragments arranged by size: 2573 1547 1178 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4185 5143 442 Size: 416 53 83 399 24 2768 958 1753 Fragments arranged by size: 2768 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3257 1056 Size: 2201 4253 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2749 5114 1239 Size: 1510 2365 2579 Fragments arranged by size: 2579 2365 1510 Bpu10I CC'TnA_GC Cuts at: 2925 2925 Size: 6454 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4579 5444 5742 4579 Size: 865 298 5291 Fragments arranged by size: 5291 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5117 882 Size: 617 3618 2219 Fragments arranged by size: 3618 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4245 493 Size: 711 3041 2702 Fragments arranged by size: 3041 2702 711 BsaBI GATnn'nnATC Cuts at: 6448 6448 Size: 6454 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4261 4643 5278 275 Size: 53 83 186 936 2728 382 635 1451 Fragments arranged by size: 2728 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4887 5285 5559 5706 6117 4887 Size: 398 274 147 411 5224 Fragments arranged by size: 5224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6229 Fragments arranged by size: 6229 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2951 1186 Size: 1765 4689 BseYI C'CCAG_C Cuts at: 2466 5657 6153 2466 Size: 3191 496 2767 Fragments arranged by size: 3191 2767 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5881 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3904 4665 5693 1540 Size: 275 186 1903 761 1028 2301 Fragments arranged by size: 2301 1903 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4515 4600 5671 4515 Size: 85 1071 5298 Fragments arranged by size: 5298 1071 85 BsmI GAATG_Cn' Cuts at: 3965 4058 3965 Size: 93 6361 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2968 3773 4515 4600 5671 1391 Size: 399 1178 805 742 85 1071 2174 Fragments arranged by size: 2174 1178 1071 805 742 399 85 BspEI T'CCGG_A Cuts at: 6117 6117 Size: 6454 BspHI T'CATG_A Cuts at: 4343 6073 4343 Size: 1730 4724 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3809 844 Size: 2965 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4341 1394 Size: 429 2518 3507 Fragments arranged by size: 3507 2518 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3756 4949 5123 65 Size: 1183 2508 1193 174 1396 Fragments arranged by size: 2508 1396 1193 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5098 5285 1700 Size: 110 3288 187 2869 Fragments arranged by size: 3288 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6454 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6454 BssSI C'ACGA_G Cuts at: 4514 5526 4514 Size: 1012 5442 BstAPI GCAn_nnn'nTGC Cuts at: 794 3206 3266 794 Size: 2412 60 3982 Fragments arranged by size: 3982 2412 60 BstBI TT'CG_AA Cuts at: 5258 5258 Size: 6454 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6454 BstYI r'GATC_y Cuts at: 1972 2593 3784 4151 4536 4553 6005 6091 6103 Size: 621 1191 367 385 17 1452 86 12 Cuts at: 6103 6449 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1191 621 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5902 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5715 Fragments arranged by size: 5715 683 56 BtsI GCAGTG_nn' Cuts at: 3276 3970 4764 4784 3276 Size: 694 794 20 4946 Fragments arranged by size: 4946 794 694 20 ClaI AT'CG_AT Cuts at: 1554 3735 1554 Size: 2181 4273 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6419 DraI TTT'AAA Cuts at: 3758 3758 Size: 6454 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5461 809 Size: 507 4145 1802 Fragments arranged by size: 4145 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3901 4790 6419 Size: 54 1425 50 275 18 2071 889 1629 Cuts at: 6419 8 Size: 43 Fragments arranged by size: 2071 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3901 1537 Size: 275 2089 4090 Fragments arranged by size: 4090 2089 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2312 3033 3063 3189 3315 3381 4384 1353 Size: 959 721 30 126 126 66 1003 3423 Fragments arranged by size: 3423 1003 959 721 126 126 66 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5041 5415 5561 387 Size: 1064 138 3452 374 146 1280 Fragments arranged by size: 3452 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2600 2749 3213 3507 3572 4517 Size: 393 651 317 149 464 294 65 945 Cuts at: 4517 5114 5901 6169 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 945 787 651 597 464 393 317 294 268 149 65 EcoRI G'AATT_C Cuts at: 3272 3763 4141 3272 Size: 491 378 5585 Fragments arranged by size: 5585 491 378 FspI TGC'GCA Cuts at: 4960 4960 Size: 6454 HaeII r_GCGC'y Cuts at: 1678 5601 1678 Size: 3923 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2908 2940 3188 3220 3226 Size: 32 135 32 1336 32 248 32 6 Cuts at: 3226 3258 5095 5127 5169 5201 1373 Size: 32 1837 32 42 32 2626 Fragments arranged by size: 2626 1837 1336 248 135 42 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 2614 2706 3798 4044 669 Size: 988 281 676 92 1092 246 3079 Fragments arranged by size: 3079 1092 988 676 281 246 92 HindIII A'AGCT_T Cuts at: 748 3348 748 Size: 2600 3854 HpaI GTT'AAC Cuts at: 2706 4044 2706 Size: 1338 5116 KpnI G_GTAC'C Cuts at: 3779 3779 Size: 6454 MfeI C'AATT_G Cuts at: 4053 4053 Size: 6454 MluI A'CGCG_T Cuts at: 6330 6330 Size: 6454 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2937 3470 5568 5752 1305 Size: 1632 533 2098 184 2007 Fragments arranged by size: 2098 2007 1632 533 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4632 Fragments arranged by size: 4632 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4412 4771 4930 518 Size: 648 3246 359 159 2042 Fragments arranged by size: 3246 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3870 4547 5695 5940 1606 Size: 2264 677 1148 245 2120 Fragments arranged by size: 2264 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5902 NdeI CA'TA_TG Cuts at: 387 387 Size: 6454 NheI G'CTAG_C Cuts at: 1052 3253 1052 Size: 2201 4253 NotI GC'GGCC_GC Cuts at: 3901 3901 Size: 6454 NspI r_CATG'y Cuts at: 1132 1229 2881 3812 5357 1132 Size: 97 1652 931 1545 2229 Fragments arranged by size: 2229 1652 1545 931 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6454 PciI A'CATG_T Cuts at: 5353 5353 Size: 6454 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6454 PmeI GTTT'AAAC Cuts at: 3758 3758 Size: 6454 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4523 4555 6036 6068 4523 Size: 32 1481 32 4909 Fragments arranged by size: 4909 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6454 PsiI TTA'TAA Cuts at: 2774 4024 2774 Size: 1250 5204 PstI C_TGCA'G Cuts at: 830 2148 2371 3806 830 Size: 1318 223 1435 3478 Fragments arranged by size: 3478 1435 1318 223 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6454 SalI G'TCGA_C Cuts at: 1936 3796 1936 Size: 1860 4594 SapI GCTCTTCn'nnn_ Cuts at: 3033 3063 3033 Size: 30 6424 SbfI CC_TGCA'GG Cuts at: 3806 3806 Size: 6454 ScaI AGT'ACT Cuts at: 4702 6414 4702 Size: 1712 4742 SexAI A'CCwGG_T Cuts at: 2097 2097 Size: 6454 SfcI C'TryA_G Cuts at: 826 1045 2144 2367 3802 4937 5618 5809 826 Size: 219 1099 223 1435 1135 681 191 1471 Fragments arranged by size: 1471 1435 1135 1099 681 223 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6454 SmaI CCC'GGG Cuts at: 1872 3781 1872 Size: 1909 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4558 5459 5721 820 Size: 197 926 2615 901 262 1553 Fragments arranged by size: 2615 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4245 493 Size: 3752 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6454 SphI G_CATG'C Cuts at: 1132 3812 1132 Size: 2680 3774 SspI AAT'ATT Cuts at: 5 52 2683 2746 4378 5 Size: 47 2631 63 1632 2081 Fragments arranged by size: 2631 2081 1632 63 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6454 StyI C'CwwG_G Cuts at: 513 1065 3893 513 Size: 552 2828 3074 Fragments arranged by size: 3074 2828 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4481 4498 4651 4836 5281 325 Size: 1255 2901 17 153 185 445 1498 Fragments arranged by size: 2901 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2908 3687 4700 Size: 274 80 33 51 1443 931 779 1013 Cuts at: 4700 6412 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1013 931 779 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6414 6446 6414 Size: 32 6422 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6454 XbaI T'CTAG_A Cuts at: 2596 3121 3295 3790 2596 Size: 525 174 495 5260 Fragments arranged by size: 5260 525 495 174 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2402 2402 Size: 6454 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6454 XmaI C'CCGG_G Cuts at: 1870 3779 1870 Size: 1909 4545 XmnI GAAnn'nnTTC Cuts at: 2283 2746 4583 2283 Size: 463 1837 4154 Fragments arranged by size: 4154 1837 463 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4262 276 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI