Restriction map for pFN21AB6120
 (Circular) (Six-base) MAPSORT of: pFN21AB6120.seq  Check: 2821  from: 1  to: 6454



pFN21AB6120  6454 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:30  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4264     278 
   Size:         53      83     186     936    2728    2468

  Fragments arranged by size: 

                2728    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2154    3797    1937 
   Size:        217    1643    4594

  Fragments arranged by size: 

                4594    1643     217
 
Acc65I G'GTAC_C

Cuts at:   3775    3775 
   Size:       6454
 
AclI AA'CG_TT

Cuts at:   1563    4581    4954    1563 
   Size:       3018     373    3063

  Fragments arranged by size: 

                3063    3018     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2283    2600    3213    3507    3572    4517    5901    6169 
   Size:        651     317     613     294      65     945    1384     268

Cuts at:   6169    1632 
   Size:       1917

  Fragments arranged by size: 

                1917    1384     945     651     613     317     294     268
                  65
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6257
 
AflIII A'CryG_T

Cuts at:   1203    3544    5353    6330    1203 
   Size:       2341    1809     977    1327

  Fragments arranged by size: 

                2341    1809    1327     977
 
AgeI A'CCGG_T

Cuts at:   5285    5285 
   Size:       6454
 
AhdI GACnn_n'nnGTC

Cuts at:   5183    5183 
   Size:       6454
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6454
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6454
 
AlwNI CAG_nnn'CTG

Cuts at:   2366    4197    5769    2366 
   Size:       1831    1572    3051

  Fragments arranged by size: 

                3051    1831    1572
 
ApaLI G'TGCA_C

Cuts at:   4511    5667    4511 
   Size:       1156    5298
 
ApoI r'AATT_y

Cuts at:   1493    2054    2252    2765    3272    3763    3989    4141    6250 
   Size:        561     198     513     507     491     226     152    2109

Cuts at:   6250    1493 
   Size:       1697

  Fragments arranged by size: 

                2109    1697     561     513     507     491     226     198
                 152
 
AseI AT'TA_AT

Cuts at:    160    1032    2282    5008     160 
   Size:        872    1250    2726    1606

  Fragments arranged by size: 

                2726    1606    1250     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6454
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3779    1870 
   Size:         73    1836    4545

  Fragments arranged by size: 

                4545    1836      73
 
BamHI G'GATC_C

Cuts at:   3784    4151    3784 
   Size:        367    6087
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2794    3775    5230     618 
   Size:        325     217     627    1007     981    1455    1842

  Fragments arranged by size: 

                1842    1455    1007     981     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3773    1391 
   Size:       2382    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3372     928 
   Size:        971    1473    4010

  Fragments arranged by size: 

                4010    1473     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4645    4679    1903 
   Size:         34      50      34    2624      34    3678

  Fragments arranged by size: 

                3678    2624      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4346    5556    4346 
   Size:       1210    5244
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6454
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5065     136 
   Size:        107     122      71     631     605    3393    1525

  Fragments arranged by size: 

                3393    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2593    6449    2593 
   Size:       3856    2598
 
BlpI GC'TnA_GC

Cuts at:   2046    3871    2046 
   Size:       1825    4629
 
Bme1580I G_kGCm'C

Cuts at:   1790    2968    4515    5671    1790 
   Size:       1178    1547    1156    2573

  Fragments arranged by size: 

                2573    1547    1178    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6454
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4185    5143     442 
   Size:        416      53      83     399      24    2768     958    1753

  Fragments arranged by size: 

                2768    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    3257    1056 
   Size:       2201    4253
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2749    5114    1239 
   Size:       1510    2365    2579

  Fragments arranged by size: 

                2579    2365    1510
 
Bpu10I CC'TnA_GC

Cuts at:   2925    2925 
   Size:       6454
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4579    5444    5742    4579 
   Size:        865     298    5291

  Fragments arranged by size: 

                5291     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5117     882 
   Size:        617    3618    2219

  Fragments arranged by size: 

                3618    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4245     493 
   Size:        711    3041    2702

  Fragments arranged by size: 

                3041    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6448    6448 
   Size:       6454
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4261    4643    5278     275 
   Size:         53      83     186     936    2728     382     635    1451

  Fragments arranged by size: 

                2728    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4887    5285    5559    5706    6117    4887 
   Size:        398     274     147     411    5224

  Fragments arranged by size: 

                5224     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6229

  Fragments arranged by size: 

                6229     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2951    1186 
   Size:       1765    4689
 
BseYI C'CCAG_C

Cuts at:   2466    5657    6153    2466 
   Size:       3191     496    2767

  Fragments arranged by size: 

                3191    2767     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5881
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3904    4665    5693    1540 
   Size:        275     186    1903     761    1028    2301

  Fragments arranged by size: 

                2301    1903    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   4515    4600    5671    4515 
   Size:         85    1071    5298

  Fragments arranged by size: 

                5298    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3965    4058    3965 
   Size:         93    6361
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2968    3773    4515    4600    5671    1391 
   Size:        399    1178     805     742      85    1071    2174

  Fragments arranged by size: 

                2174    1178    1071     805     742     399      85
 
BspEI T'CCGG_A

Cuts at:   6117    6117 
   Size:       6454
 
BspHI T'CATG_A

Cuts at:   4343    6073    4343 
   Size:       1730    4724
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3809     844 
   Size:       2965    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4341    1394 
   Size:        429    2518    3507

  Fragments arranged by size: 

                3507    2518     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3756    4949    5123      65 
   Size:       1183    2508    1193     174    1396

  Fragments arranged by size: 

                2508    1396    1193    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5098    5285    1700 
   Size:        110    3288     187    2869

  Fragments arranged by size: 

                3288    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6454
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6454
 
BssSI C'ACGA_G

Cuts at:   4514    5526    4514 
   Size:       1012    5442
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3206    3266     794 
   Size:       2412      60    3982

  Fragments arranged by size: 

                3982    2412      60
 
BstBI TT'CG_AA

Cuts at:   5258    5258 
   Size:       6454
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6454
 
BstYI r'GATC_y

Cuts at:   1972    2593    3784    4151    4536    4553    6005    6091    6103 
   Size:        621    1191     367     385      17    1452      86      12

Cuts at:   6103    6449    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452    1191     621     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5902
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5715

  Fragments arranged by size: 

                5715     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3276    3970    4764    4784    3276 
   Size:        694     794      20    4946

  Fragments arranged by size: 

                4946     794     694      20
 
ClaI AT'CG_AT

Cuts at:   1554    3735    1554 
   Size:       2181    4273
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6419
 
DraI TTT'AAA

Cuts at:   3758    3758 
   Size:       6454
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5461     809 
   Size:        507    4145    1802

  Fragments arranged by size: 

                4145    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3901    4790    6419 
   Size:         54    1425      50     275      18    2071     889    1629

Cuts at:   6419       8 
   Size:         43

  Fragments arranged by size: 

                2071    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3901    1537 
   Size:        275    2089    4090

  Fragments arranged by size: 

                4090    2089     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2312    3033    3063    3189    3315    3381    4384    1353 
   Size:        959     721      30     126     126      66    1003    3423

  Fragments arranged by size: 

                3423    1003     959     721     126     126      66      30
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5041    5415    5561     387 
   Size:       1064     138    3452     374     146    1280

  Fragments arranged by size: 

                3452    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2283    2600    2749    3213    3507    3572    4517 
   Size:        393     651     317     149     464     294      65     945

Cuts at:   4517    5114    5901    6169    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524     945     787     651     597     464     393     317
                 294     268     149      65
 
EcoRI G'AATT_C

Cuts at:   3272    3763    4141    3272 
   Size:        491     378    5585

  Fragments arranged by size: 

                5585     491     378
 
FspI TGC'GCA

Cuts at:   4960    4960 
   Size:       6454
 
HaeII r_GCGC'y

Cuts at:   1678    5601    1678 
   Size:       3923    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2908    2940    3188    3220    3226 
   Size:         32     135      32    1336      32     248      32       6

Cuts at:   3226    3258    5095    5127    5169    5201    1373 
   Size:         32    1837      32      42      32    2626

  Fragments arranged by size: 

                2626    1837    1336     248     135      42      32      32
                  32      32      32      32      32       6
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2614    2706    3798    4044     669 
   Size:        988     281     676      92    1092     246    3079

  Fragments arranged by size: 

                3079    1092     988     676     281     246      92
 
HindIII A'AGCT_T

Cuts at:    748    3348     748 
   Size:       2600    3854
 
HpaI GTT'AAC

Cuts at:   2706    4044    2706 
   Size:       1338    5116
 
KpnI G_GTAC'C

Cuts at:   3779    3779 
   Size:       6454
 
MfeI C'AATT_G

Cuts at:   4053    4053 
   Size:       6454
 
MluI A'CGCG_T

Cuts at:   6330    6330 
   Size:       6454
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2937    3470    5568    5752    1305 
   Size:       1632     533    2098     184    2007

  Fragments arranged by size: 

                2098    2007    1632     533     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4632

  Fragments arranged by size: 

                4632    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    4412    4771    4930     518 
   Size:        648    3246     359     159    2042

  Fragments arranged by size: 

                3246    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3870    4547    5695    5940    1606 
   Size:       2264     677    1148     245    2120

  Fragments arranged by size: 

                2264    2120    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5902
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6454
 
NheI G'CTAG_C

Cuts at:   1052    3253    1052 
   Size:       2201    4253
 
NotI GC'GGCC_GC

Cuts at:   3901    3901 
   Size:       6454
 
NspI r_CATG'y

Cuts at:   1132    1229    2881    3812    5357    1132 
   Size:         97    1652     931    1545    2229

  Fragments arranged by size: 

                2229    1652    1545     931      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6454
 
PciI A'CATG_T

Cuts at:   5353    5353 
   Size:       6454
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6454
 
PmeI GTTT'AAAC

Cuts at:   3758    3758 
   Size:       6454
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4523    4555    6036    6068    4523 
   Size:         32    1481      32    4909

  Fragments arranged by size: 

                4909    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6454
 
PsiI TTA'TAA

Cuts at:   2774    4024    2774 
   Size:       1250    5204
 
PstI C_TGCA'G

Cuts at:    830    2148    2371    3806     830 
   Size:       1318     223    1435    3478

  Fragments arranged by size: 

                3478    1435    1318     223
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6454
 
SalI G'TCGA_C

Cuts at:   1936    3796    1936 
   Size:       1860    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   3033    3063    3033 
   Size:         30    6424
 
SbfI CC_TGCA'GG

Cuts at:   3806    3806 
   Size:       6454
 
ScaI AGT'ACT

Cuts at:   4702    6414    4702 
   Size:       1712    4742
 
SexAI A'CCwGG_T

Cuts at:   2097    2097 
   Size:       6454
 
SfcI C'TryA_G

Cuts at:    826    1045    2144    2367    3802    4937    5618    5809     826 
   Size:        219    1099     223    1435    1135     681     191    1471

  Fragments arranged by size: 

                1471    1435    1135    1099     681     223     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6454
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6454
 
SmaI CCC'GGG

Cuts at:   1872    3781    1872 
   Size:       1909    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4558    5459    5721     820 
   Size:        197     926    2615     901     262    1553

  Fragments arranged by size: 

                2615    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4245     493 
   Size:       3752    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6454
 
SphI G_CATG'C

Cuts at:   1132    3812    1132 
   Size:       2680    3774
 
SspI AAT'ATT

Cuts at:      5      52    2683    2746    4378       5 
   Size:         47    2631      63    1632    2081

  Fragments arranged by size: 

                2631    2081    1632      63      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6454
 
StyI C'CwwG_G

Cuts at:    513    1065    3893     513 
   Size:        552    2828    3074

  Fragments arranged by size: 

                3074    2828     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4481    4498    4651    4836    5281     325 
   Size:       1255    2901      17     153     185     445    1498

  Fragments arranged by size: 

                2901    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2908    3687    4700 
   Size:        274      80      33      51    1443     931     779    1013

Cuts at:   4700    6412      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1013     931     779     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6414    6446    6414 
   Size:         32    6422
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6454
 
XbaI T'CTAG_A

Cuts at:   2596    3121    3295    3790    2596 
   Size:        525     174     495    5260

  Fragments arranged by size: 

                5260     525     495     174
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2402    2402 
   Size:       6454
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6454
 
XmaI C'CCGG_G

Cuts at:   1870    3779    1870 
   Size:       1909    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2283    2746    4583    2283 
   Size:        463    1837    4154

  Fragments arranged by size: 

                4154    1837     463
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4262     276 
   Size:         53      83     186     936    2728    2468

  Fragments arranged by size: 

                2728    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII    SanDI     SfiI     SfoI     SrfI     SwaI
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