(Circular) (Six-base) MAPSORT of: pFN21AB6117.seq Check: 3169 from: 1 to: 6568 pFN21AB6117 6568 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4378 278 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3911 1937 Size: 1974 4594 Acc65I G'GTAC_C Cuts at: 3889 3889 Size: 6568 AclI AA'CG_TT Cuts at: 1563 4695 5068 1563 Size: 3132 373 3063 Fragments arranged by size: 3132 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2310 2799 4631 6015 6283 1632 Size: 678 489 1832 1384 268 1917 Fragments arranged by size: 1917 1832 1384 678 489 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6371 AflIII A'CryG_T Cuts at: 1203 5467 6444 1203 Size: 4264 977 1327 Fragments arranged by size: 4264 1327 977 AgeI A'CCGG_T Cuts at: 5399 5399 Size: 6568 AhdI GACnn_n'nnGTC Cuts at: 5297 5297 Size: 6568 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6568 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6568 AlwNI CAG_nnn'CTG Cuts at: 2996 3374 4311 5883 2996 Size: 378 937 1572 3681 Fragments arranged by size: 3681 1572 937 378 ApaLI G'TGCA_C Cuts at: 4625 5781 4625 Size: 1156 5412 ApoI r'AATT_y Cuts at: 1493 2832 3219 3254 3877 4103 4255 6364 1493 Size: 1339 387 35 623 226 152 2109 1697 Fragments arranged by size: 2109 1697 1339 623 387 226 152 35 AseI AT'TA_AT Cuts at: 160 1032 5122 160 Size: 872 4090 1606 Fragments arranged by size: 4090 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 AvaI C'yCGr_G Cuts at: 1870 1943 3893 1870 Size: 73 1950 4545 Fragments arranged by size: 4545 1950 73 BamHI G'GATC_C Cuts at: 3898 4265 3898 Size: 367 6201 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3544 3889 5344 618 Size: 325 217 627 1757 345 1455 1842 Fragments arranged by size: 1842 1757 1455 627 345 325 217 BanII G_rGCy'C Cuts at: 1391 3887 1391 Size: 2496 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3456 928 Size: 971 1557 4040 Fragments arranged by size: 4040 1557 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4759 4793 1903 Size: 34 2822 34 3678 Fragments arranged by size: 3678 2822 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3040 3322 4460 5670 3040 Size: 282 1138 1210 3938 Fragments arranged by size: 3938 1210 1138 282 BclI T'GATC_A Cuts at: 1548 2953 1548 Size: 1405 5163 BfrBI ATG'CAT Cuts at: 2580 3474 2580 Size: 894 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3386 5179 136 Size: 107 122 71 631 605 1714 1793 1525 Fragments arranged by size: 1793 1714 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6563 6563 Size: 6568 BlpI GC'TnA_GC Cuts at: 3985 3985 Size: 6568 Bme1580I G_kGCm'C Cuts at: 1790 3044 3549 4629 5785 1790 Size: 1254 505 1080 1156 2573 Fragments arranged by size: 2573 1254 1156 1080 505 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6568 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3543 3682 4299 Size: 416 53 83 399 24 2126 139 617 Cuts at: 4299 5257 442 Size: 958 1753 Fragments arranged by size: 2126 1753 958 617 416 399 139 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6568 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5228 1239 Size: 3989 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2652 4693 5558 5856 2652 Size: 2041 865 298 3364 Fragments arranged by size: 3364 2041 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3280 5231 882 Size: 617 1781 1951 2219 Fragments arranged by size: 2219 1951 1781 617 BsaAI yAC'GTr Cuts at: 493 1204 4359 493 Size: 711 3155 2702 Fragments arranged by size: 3155 2702 711 BsaBI GATnn'nnATC Cuts at: 2171 2679 6562 2171 Size: 508 3883 2177 Fragments arranged by size: 3883 2177 508 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2191 2779 4375 4757 Size: 53 83 186 936 658 588 1596 382 Cuts at: 4757 5392 275 Size: 635 1451 Fragments arranged by size: 1596 1451 936 658 635 588 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5001 5399 5673 5820 6231 5001 Size: 398 274 147 411 5338 Fragments arranged by size: 5338 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6343 Fragments arranged by size: 6343 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6568 BseYI C'CCAG_C Cuts at: 5771 6267 5771 Size: 496 6072 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2462 3099 1163 Size: 573 726 637 4632 Fragments arranged by size: 4632 726 637 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4018 4779 5807 1540 Size: 275 186 2017 761 1028 2301 Fragments arranged by size: 2301 2017 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2968 4629 4714 5785 2968 Size: 1661 85 1071 3751 Fragments arranged by size: 3751 1661 1071 85 BsmI GAATG_Cn' Cuts at: 4079 4172 4079 Size: 93 6475 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2968 3044 3549 3887 4629 4714 5785 Size: 399 1178 76 505 338 742 85 1071 Cuts at: 5785 1391 Size: 2174 Fragments arranged by size: 2174 1178 1071 742 505 399 338 85 76 BspEI T'CCGG_A Cuts at: 6231 6231 Size: 6568 BspHI T'CATG_A Cuts at: 4457 6187 4457 Size: 1730 4838 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3923 844 Size: 3079 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2145 4455 1394 Size: 429 322 2310 3507 Fragments arranged by size: 3507 2310 429 322 BsrDI GCAATG_nn' Cuts at: 65 1248 5063 5237 65 Size: 1183 3815 174 1396 Fragments arranged by size: 3815 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5212 5399 1700 Size: 110 3402 187 2869 Fragments arranged by size: 3402 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3089 3326 96 Size: 2993 237 3338 Fragments arranged by size: 3338 2993 237 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6568 BssSI C'ACGA_G Cuts at: 2494 4628 5640 2494 Size: 2134 1012 3422 Fragments arranged by size: 3422 2134 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6568 BstBI TT'CG_AA Cuts at: 2016 5372 2016 Size: 3356 3212 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6568 BstXI CCAn_nnnn'nTGG Cuts at: 3266 3266 Size: 6568 BstYI r'GATC_y Cuts at: 1972 2992 3898 4265 4650 4667 6119 6205 6217 Size: 1020 906 367 385 17 1452 86 12 Cuts at: 6217 6563 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1020 906 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2295 513 Size: 552 939 291 4786 Fragments arranged by size: 4786 939 552 291 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5829 Fragments arranged by size: 5829 683 56 BtsI GCAGTG_nn' Cuts at: 3087 3107 3838 4084 4878 4898 3087 Size: 20 731 246 794 20 4757 Fragments arranged by size: 4757 794 731 246 20 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6568 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6533 DraI TTT'AAA Cuts at: 3872 3872 Size: 6568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5575 809 Size: 507 4259 1802 Fragments arranged by size: 4259 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2292 4015 4904 Size: 54 1425 50 275 18 462 1723 889 Cuts at: 4904 6533 8 Size: 1629 43 Fragments arranged by size: 1723 1629 1425 889 462 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4015 1537 Size: 275 2203 4090 Fragments arranged by size: 4090 2203 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2013 2135 3438 3439 4498 1353 Size: 660 122 1303 1 1059 3423 Fragments arranged by size: 3423 1303 1059 660 122 1 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3570 5155 5529 5675 387 Size: 1064 138 435 1546 1585 374 146 1280 Fragments arranged by size: 1585 1546 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 2799 4631 5228 6015 6283 1239 Size: 393 678 489 1832 597 787 268 1524 Fragments arranged by size: 1832 1524 787 678 597 489 393 268 EcoRI G'AATT_C Cuts at: 3877 4255 3877 Size: 378 6190 EcoRV GAT'ATC Cuts at: 2198 2198 Size: 6568 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2999 2999 Size: 6568 FspI TGC'GCA Cuts at: 5074 5074 Size: 6568 HaeII r_GCGC'y Cuts at: 1678 2129 2773 5715 1678 Size: 451 644 2942 2531 Fragments arranged by size: 2942 2531 644 451 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 3842 3874 5209 Size: 32 135 32 680 32 1558 32 1335 Cuts at: 5209 5241 5283 5315 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1558 1335 680 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3912 4158 669 Size: 988 281 1974 246 3079 Fragments arranged by size: 3079 1974 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6568 HpaI GTT'AAC Cuts at: 4158 4158 Size: 6568 KpnI G_GTAC'C Cuts at: 3893 3893 Size: 6568 MfeI C'AATT_G Cuts at: 4167 4167 Size: 6568 MluI A'CGCG_T Cuts at: 6444 6444 Size: 6568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5682 5866 1305 Size: 4377 184 2007 Fragments arranged by size: 4377 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4746 Fragments arranged by size: 4746 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2436 3479 4526 4885 5044 518 Size: 648 1270 1043 1047 359 159 2042 Fragments arranged by size: 2042 1270 1047 1043 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2819 3984 4661 5809 6054 1606 Size: 1213 1165 677 1148 245 2120 Fragments arranged by size: 2120 1213 1165 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5077 Fragments arranged by size: 5077 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6568 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6568 NotI GC'GGCC_GC Cuts at: 4015 4015 Size: 6568 NsiI A_TGCA'T Cuts at: 2582 3476 2582 Size: 894 5674 NspI r_CATG'y Cuts at: 1132 1229 3637 3926 5471 1132 Size: 97 2408 289 1545 2229 Fragments arranged by size: 2408 2229 1545 289 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6568 PciI A'CATG_T Cuts at: 5467 5467 Size: 6568 PflMI CCAn_nnn'nTGG Cuts at: 1108 2844 1108 Size: 1736 4832 PmeI GTTT'AAAC Cuts at: 3872 3872 Size: 6568 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4637 4669 6150 6182 4637 Size: 32 1481 32 5023 Fragments arranged by size: 5023 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3818 1613 Size: 2205 4363 PsiI TTA'TAA Cuts at: 4138 4138 Size: 6568 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3349 3381 3349 Size: 32 6536 PstI C_TGCA'G Cuts at: 830 2153 2992 3102 3920 830 Size: 1323 839 110 818 3478 Fragments arranged by size: 3478 1323 839 818 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6568 PvuII CAG'CTG Cuts at: 2819 2819 Size: 6568 SalI G'TCGA_C Cuts at: 1936 3910 1936 Size: 1974 4594 SapI GCTCTTCn'nnn_ Cuts at: 2135 3439 2135 Size: 1304 5264 SbfI CC_TGCA'GG Cuts at: 3920 3920 Size: 6568 ScaI AGT'ACT Cuts at: 3692 3781 4816 6528 3692 Size: 89 1035 1712 3732 Fragments arranged by size: 3732 1712 1035 89 SfcI C'TryA_G Cuts at: 826 1045 2149 2277 2988 3098 3375 3916 5051 Size: 219 1104 128 711 110 277 541 1135 Cuts at: 5051 5732 5923 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1104 711 681 541 277 219 191 128 110 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6568 SmaI CCC'GGG Cuts at: 1872 3895 1872 Size: 2023 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2667 4672 5573 5835 820 Size: 197 926 724 2005 901 262 1553 Fragments arranged by size: 2005 1553 926 901 724 262 197 SnaBI TAC'GTA Cuts at: 493 4359 493 Size: 3866 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6568 SphI G_CATG'C Cuts at: 1132 3926 1132 Size: 2794 3774 SspI AAT'ATT Cuts at: 5 52 2791 3198 4492 5 Size: 47 2739 407 1294 2081 Fragments arranged by size: 2739 2081 1294 407 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6568 StyI C'CwwG_G Cuts at: 513 1065 2004 4007 513 Size: 552 939 2003 3074 Fragments arranged by size: 3074 2003 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3304 4595 4612 4765 4950 5395 325 Size: 1255 1724 1291 17 153 185 445 1498 Fragments arranged by size: 1724 1498 1291 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3089 3326 3690 Size: 274 80 33 51 1443 1112 237 364 Cuts at: 3690 3779 4814 6526 96 Size: 89 1035 1712 138 Fragments arranged by size: 1712 1443 1112 1035 364 274 237 138 89 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2080 2112 2330 2362 6528 6560 2080 Size: 32 218 32 4166 32 2088 Fragments arranged by size: 4166 2088 218 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6568 XbaI T'CTAG_A Cuts at: 3904 3904 Size: 6568 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6568 XmaI C'CCGG_G Cuts at: 1870 3893 1870 Size: 2023 4545 XmnI GAAnn'nnTTC Cuts at: 4697 4697 Size: 6568 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4376 276 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI