Restriction map for
pFN21AB6117
(Linear) (Six-base) MAPSORT of: KIBB6117.seq Check: 7635 from: 1 to: 1863
KIBB6117.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 794 1863
Size: 305 489 1069
Fragments arranged by size:
1069 489 305
AlwNI CAG_nnn'CTG
Cuts at: 0 991 1369 1863
Size: 991 378 494
Fragments arranged by size:
991 494 378
ApoI r'AATT_y
Cuts at: 0 827 1214 1249 1863
Size: 827 387 35 614
Fragments arranged by size:
827 614 387 35
BanI G'GyrC_C
Cuts at: 0 1539 1863
Size: 1539 324
BbsI GAAGACnn'nnnn_
Cuts at: 0 1451 1863
Size: 1451 412
BciVI GTATCCnnnnn_n'
Cuts at: 0 1035 1317 1863
Size: 1035 282 546
Fragments arranged by size:
1035 546 282
BclI T'GATC_A
Cuts at: 0 948 1863
Size: 948 915
BfrBI ATG'CAT
Cuts at: 0 575 1469 1863
Size: 575 894 394
Fragments arranged by size:
894 575 394
BglI GCCn_nnn'nGGC
Cuts at: 0 1381 1863
Size: 1381 482
Bme1580I G_kGCm'C
Cuts at: 0 1039 1544 1863
Size: 1039 505 319
Fragments arranged by size:
1039 505 319
BmrI ACTGGGnnnn_n'
Cuts at: 0 1538 1677 1863
Size: 1538 139 186
Fragments arranged by size:
1538 186 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 1863
Size: 647 1216
BsaI GGTCTCn'nnnn_
Cuts at: 0 1275 1863
Size: 1275 588
BsaBI GATnn'nnATC
Cuts at: 0 166 674 1863
Size: 166 508 1189
Fragments arranged by size:
1189 508 166
BsaHI Gr'CG_yC
Cuts at: 0 186 774 1863
Size: 186 588 1089
Fragments arranged by size:
1089 588 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1094 1863
Size: 457 637 769
Fragments arranged by size:
769 637 457
BsiHKAI G_wGCw'C
Cuts at: 0 963 1863
Size: 963 900
Bsp1286I G_dGCh'C
Cuts at: 0 963 1039 1544 1863
Size: 963 76 505 319
Fragments arranged by size:
963 505 319 76
BsrBI CCG'CTC
Cuts at: 0 140 1863
Size: 140 1723
BsrGI T'GTAC_A
Cuts at: 0 1084 1321 1863
Size: 1084 237 542
Fragments arranged by size:
1084 542 237
BssSI C'ACGA_G
Cuts at: 0 489 1863
Size: 489 1374
BstBI TT'CG_AA
Cuts at: 0 11 1863
Size: 11 1852
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1261 1863
Size: 1261 602
BstYI r'GATC_y
Cuts at: 0 987 1863
Size: 987 876
BtgI C'CryG_G
Cuts at: 0 290 1863
Size: 290 1573
BtsI GCAGTG_nn'
Cuts at: 0 1082 1102 1833 1863
Size: 1082 20 731 30
Fragments arranged by size:
1082 731 30 20
EaeI y'GGCC_r
Cuts at: 0 287 1863
Size: 287 1576
EarI CTCTTCn'nnn_
Cuts at: 0 8 130 1433 1434 1863
Size: 8 122 1303 1 429
Fragments arranged by size:
1303 429 122 8 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1565 1863
Size: 19 1546 298
Fragments arranged by size:
1546 298 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 794 1863
Size: 305 489 1069
Fragments arranged by size:
1069 489 305
EcoRV GAT'ATC
Cuts at: 0 193 1863
Size: 193 1670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 994 1863
Size: 994 869
HaeII r_GCGC'y
Cuts at: 0 124 768 1863
Size: 124 644 1095
Fragments arranged by size:
1095 644 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 1837 1863
Size: 247 32 1558 26
Fragments arranged by size:
1558 247 32 26
MslI CAynn'nnrTG
Cuts at: 0 431 1474 1863
Size: 431 1043 389
Fragments arranged by size:
1043 431 389
MspA1I CmG'CkG
Cuts at: 0 814 1863
Size: 814 1049
NsiI A_TGCA'T
Cuts at: 0 577 1471 1863
Size: 577 894 392
Fragments arranged by size:
894 577 392
NspI r_CATG'y
Cuts at: 0 1632 1863
Size: 1632 231
PflMI CCAn_nnn'nTGG
Cuts at: 0 839 1863
Size: 839 1024
PshAI GACnn'nnGTC
Cuts at: 0 1813 1863
Size: 1813 50
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1344 1376 1863
Size: 1344 32 487
Fragments arranged by size:
1344 487 32
PstI C_TGCA'G
Cuts at: 0 148 987 1097 1863
Size: 148 839 110 766
Fragments arranged by size:
839 766 148 110
PvuII CAG'CTG
Cuts at: 0 814 1863
Size: 814 1049
SapI GCTCTTCn'nnn_
Cuts at: 0 130 1434 1863
Size: 130 1304 429
Fragments arranged by size:
1304 429 130
ScaI AGT'ACT
Cuts at: 0 1687 1776 1863
Size: 1687 89 87
Fragments arranged by size:
1687 89 87
SfcI C'TryA_G
Cuts at: 0 144 272 983 1093 1370 1863
Size: 144 128 711 110 277 493
Fragments arranged by size:
711 493 277 144 128 110
SmlI C'TyrA_G
Cuts at: 0 662 1863
Size: 662 1201
SspI AAT'ATT
Cuts at: 0 786 1193 1863
Size: 786 407 670
Fragments arranged by size:
786 670 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1299 1863
Size: 1299 564
TatI w'GTAC_w
Cuts at: 0 1084 1321 1685 1774 1863
Size: 1084 237 364 89 89
Fragments arranged by size:
1084 364 237 89 89
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 75 107 325 357 1863
Size: 75 32 218 32 1506
Fragments arranged by size:
1506 218 75 32 32
Enzymes that do cut:
AcuI AlwNI ApoI BanI BbsI BciVI BclI BfrBI
BglI Bme1580I BmrI BpuEI BsaI BsaBI BsaHI BsgI
BsiHKAI Bsp1286I BsrBI BsrGI BssSI BstBI BstXI BstYI
BtgI BtsI EaeI EarI EciI Eco57MI EcoRV FalI
HaeII Hin4I MslI MspA1I NsiI NspI PflMI PshAI
PsrI PstI PvuII SapI ScaI SfcI SmlI SspI
TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BbvCI BcgI BglII BlpI BmgBI BmtI BplI BpmI
Bpu10I BsaAI BsaWI BsaXI BseRI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BssHII
BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
StyI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI