(Circular) (Six-base) MAPSORT of: pFN21AB5960.seq Check: 6784 from: 1 to: 6358 pFN21AB5960 6358 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3085 3307 3085 Size: 222 6136 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2718 4168 278 Size: 53 83 186 936 1182 1450 2468 Fragments arranged by size: 2468 1450 1182 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2009 3375 3701 1937 Size: 72 1366 326 4594 Fragments arranged by size: 4594 1366 326 72 Acc65I G'GTAC_C Cuts at: 3679 3679 Size: 6358 AclI AA'CG_TT Cuts at: 1563 2151 4485 4858 1563 Size: 588 2334 373 3063 Fragments arranged by size: 3063 2334 588 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 4421 5805 6073 1632 Size: 710 2079 1384 268 1917 Fragments arranged by size: 2079 1917 1384 710 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6161 AflIII A'CryG_T Cuts at: 1203 2301 5257 6234 1203 Size: 1098 2956 977 1327 Fragments arranged by size: 2956 1327 1098 977 AgeI A'CCGG_T Cuts at: 5189 5189 Size: 6358 AhdI GACnn_n'nnGTC Cuts at: 5087 5087 Size: 6358 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6358 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3185 1184 Size: 2001 4357 AlwNI CAG_nnn'CTG Cuts at: 2320 2495 2735 2843 2948 3464 4101 5673 2320 Size: 175 240 108 105 516 637 1572 3005 Fragments arranged by size: 3005 1572 637 516 240 175 108 105 ApaI G_GGCC'C Cuts at: 2108 2765 2967 3018 3360 3407 3596 3626 2108 Size: 657 202 51 342 47 189 30 4840 Fragments arranged by size: 4840 657 342 202 189 51 47 30 ApaLI G'TGCA_C Cuts at: 4415 5571 4415 Size: 1156 5202 ApoI r'AATT_y Cuts at: 1493 3667 3893 4045 6154 1493 Size: 2174 226 152 2109 1697 Fragments arranged by size: 2174 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4912 160 Size: 872 3880 1606 Fragments arranged by size: 3880 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 AvaI C'yCGr_G Cuts at: 1870 1943 2516 3619 3683 1870 Size: 73 573 1103 64 4545 Fragments arranged by size: 4545 1103 573 73 64 BamHI G'GATC_C Cuts at: 2616 3688 4055 2616 Size: 1072 367 4919 Fragments arranged by size: 4919 1072 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2025 3220 3679 5134 618 Size: 325 217 627 238 1195 459 1455 1842 Fragments arranged by size: 1842 1455 1195 627 459 325 238 217 BanII G_rGCy'C Cuts at: 1391 2108 2504 2765 2967 3018 3132 3360 3407 Size: 717 396 261 202 51 114 228 47 Cuts at: 3407 3596 3626 3677 1391 Size: 189 30 51 4072 Fragments arranged by size: 4072 717 396 261 228 202 189 114 51 51 47 30 BbeI G_GCGC'C Cuts at: 2029 2029 Size: 6358 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2710 3341 928 Size: 971 811 631 3945 Fragments arranged by size: 3945 971 811 631 BbvCI CC'TCA_GC Cuts at: 3019 3276 3019 Size: 257 6101 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4549 4583 1903 Size: 34 2612 34 3678 Fragments arranged by size: 3678 2612 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4250 5460 4250 Size: 1210 5148 BclI T'GATC_A Cuts at: 1548 1548 Size: 6358 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3402 4969 136 Size: 107 122 71 631 605 1730 1567 1525 Fragments arranged by size: 1730 1567 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6353 6353 Size: 6358 BlpI GC'TnA_GC Cuts at: 2385 3775 2385 Size: 1390 4968 Bme1580I G_kGCm'C Cuts at: 1790 2108 2765 2967 3018 3360 3407 3596 3626 Size: 318 657 202 51 342 47 189 30 Cuts at: 3626 4419 5575 1790 Size: 793 1156 2573 Fragments arranged by size: 2573 1156 793 657 342 318 202 189 51 47 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6358 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2685 4089 5047 Size: 416 53 83 399 24 1268 1404 958 Cuts at: 5047 442 Size: 1753 Fragments arranged by size: 1753 1404 1268 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6358 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2552 2820 2911 3221 3438 3576 5018 1239 Size: 1313 268 91 310 217 138 1442 2579 Fragments arranged by size: 2579 1442 1313 310 268 217 138 91 Bpu10I CC'TnA_GC Cuts at: 2503 3019 3276 2503 Size: 516 257 5585 Fragments arranged by size: 5585 516 257 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2041 2432 4483 5348 5646 2041 Size: 391 2051 865 298 2753 Fragments arranged by size: 2753 2051 865 391 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5021 882 Size: 617 3522 2219 Fragments arranged by size: 3522 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2338 3570 4149 493 Size: 711 1134 1232 579 2702 Fragments arranged by size: 2702 1232 1134 711 579 BsaBI GATnn'nnATC Cuts at: 2651 6352 2651 Size: 3701 2657 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2026 2218 2715 3338 Size: 53 83 186 936 493 192 497 623 Cuts at: 3338 4165 4547 5182 275 Size: 827 382 635 1451 Fragments arranged by size: 1451 936 827 635 623 497 493 382 192 186 83 53 BsaWI w'CCGG_w Cuts at: 4791 5189 5463 5610 6021 4791 Size: 398 274 147 411 5128 Fragments arranged by size: 5128 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2288 2294 2318 2516 Size: 30 165 30 861 24 6 24 198 Cuts at: 2516 2546 2678 2708 3298 3328 1178 Size: 30 132 30 590 30 4208 Fragments arranged by size: 4208 861 590 198 165 132 30 30 30 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2567 2570 2609 2624 2672 2702 1186 Size: 1381 3 39 15 48 30 4842 Fragments arranged by size: 4842 1381 48 39 30 15 3 BseYI C'CCAG_C Cuts at: 2728 2764 2941 2954 3086 3457 5561 6057 2728 Size: 36 177 13 132 371 2104 496 3029 Fragments arranged by size: 3029 2104 496 371 177 132 36 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2333 1163 Size: 573 597 5188 Fragments arranged by size: 5188 597 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3401 3808 4569 5597 1540 Size: 275 186 1400 407 761 1028 2301 Fragments arranged by size: 2301 1400 1028 761 407 275 186 BsiHKAI G_wGCw'C Cuts at: 4419 4504 5575 4419 Size: 85 1071 5202 Fragments arranged by size: 5202 1071 85 BsmI GAATG_Cn' Cuts at: 3869 3962 3869 Size: 93 6265 BsmBI CGTCTCn'nnnn_ Cuts at: 3536 3536 Size: 6358 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2504 2765 2967 3018 3132 3360 Size: 399 318 396 261 202 51 114 228 Cuts at: 3360 3407 3596 3626 3677 4419 4504 5575 1391 Size: 47 189 30 51 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 396 318 261 228 202 189 114 85 51 51 47 30 BspEI T'CCGG_A Cuts at: 6021 6021 Size: 6358 BspHI T'CATG_A Cuts at: 2172 4247 5977 2172 Size: 2075 1730 2553 Fragments arranged by size: 2553 2075 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2643 3085 3307 3713 844 Size: 1799 442 222 406 3489 Fragments arranged by size: 3489 1799 442 406 222 BsrBI CCG'CTC Cuts at: 1394 1823 2434 2614 4245 1394 Size: 429 611 180 1631 3507 Fragments arranged by size: 3507 1631 611 429 180 BsrDI GCAATG_nn' Cuts at: 65 1248 2098 4853 5027 65 Size: 1183 850 2755 174 1396 Fragments arranged by size: 2755 1396 1183 850 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2264 2418 2872 3040 3223 3396 5002 Size: 110 454 154 454 168 183 173 1606 Cuts at: 5002 5189 1700 Size: 187 2869 Fragments arranged by size: 2869 1606 454 454 187 183 173 168 154 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6358 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6358 BssSI C'ACGA_G Cuts at: 2086 4418 5430 2086 Size: 2332 1012 3014 Fragments arranged by size: 3014 2332 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6358 BstBI TT'CG_AA Cuts at: 5162 5162 Size: 6358 BstEII G'GTnAC_C Cuts at: 1184 3072 1184 Size: 1888 4470 BstYI r'GATC_y Cuts at: 1972 2616 3058 3266 3688 4055 4440 4457 5909 Size: 644 442 208 422 367 385 17 1452 Cuts at: 5909 5995 6007 6353 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 644 442 422 385 367 346 208 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2203 2478 2772 2790 2978 513 Size: 552 1138 275 294 18 188 3893 Fragments arranged by size: 3893 1138 552 294 275 188 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5619 Fragments arranged by size: 5619 683 56 BtsI GCAGTG_nn' Cuts at: 2294 3167 3874 4668 4688 2294 Size: 873 707 794 20 3964 Fragments arranged by size: 3964 873 794 707 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6323 DraI TTT'AAA Cuts at: 3662 3662 Size: 6358 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3343 5365 809 Size: 507 2027 2022 1802 Fragments arranged by size: 2027 2022 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2534 2580 3398 Size: 54 1425 50 275 18 704 46 818 Cuts at: 3398 3805 4694 6323 8 Size: 407 889 1629 43 Fragments arranged by size: 1629 1425 889 818 704 407 275 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3398 3805 1537 Size: 275 1586 407 4090 Fragments arranged by size: 4090 1586 407 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2424 4288 1353 Size: 1071 1864 3423 Fragments arranged by size: 3423 1864 1071 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2078 2537 3465 4945 5319 5465 Size: 1064 138 489 459 928 1480 374 146 Cuts at: 5465 387 Size: 1280 Fragments arranged by size: 1480 1280 1064 928 489 459 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2552 2820 2911 3221 3438 3576 Size: 393 710 210 268 91 310 217 138 Cuts at: 3576 4421 5018 5805 6073 1239 Size: 845 597 787 268 1524 Fragments arranged by size: 1524 845 787 710 597 393 310 268 268 217 210 138 91 EcoO109I rG'GnC_Cy Cuts at: 2104 2507 2761 2884 2912 2937 2963 3001 3015 Size: 403 254 123 28 25 26 38 14 Cuts at: 3015 3356 3404 3592 3593 3623 2104 Size: 341 48 188 1 30 4839 Fragments arranged by size: 4839 403 341 254 188 123 48 38 30 28 26 25 14 1 EcoRI G'AATT_C Cuts at: 3667 4045 3667 Size: 378 5980 FspI TGC'GCA Cuts at: 4864 4864 Size: 6358 HaeII r_GCGC'y Cuts at: 1678 2029 3563 5505 1678 Size: 351 1534 1942 2531 Fragments arranged by size: 2531 1942 1534 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 2678 2710 3125 Size: 32 135 32 692 32 382 32 415 Cuts at: 3125 3157 3368 3400 4999 5031 5073 5105 1373 Size: 32 211 32 1599 32 42 32 2626 Fragments arranged by size: 2626 1599 692 415 382 211 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2010 2923 3376 3702 3948 669 Size: 988 281 72 913 453 326 246 3079 Fragments arranged by size: 3079 988 913 453 326 281 246 72 HindIII A'AGCT_T Cuts at: 748 748 Size: 6358 HpaI GTT'AAC Cuts at: 3948 3948 Size: 6358 KasI G'GCGC_C Cuts at: 2025 2025 Size: 6358 KpnI G_GTAC'C Cuts at: 3683 3683 Size: 6358 MfeI C'AATT_G Cuts at: 3957 3957 Size: 6358 MluI A'CGCG_T Cuts at: 6234 6234 Size: 6358 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3456 5472 5656 1305 Size: 2151 2016 184 2007 Fragments arranged by size: 2151 2016 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2582 10 Size: 54 1425 343 750 3786 Fragments arranged by size: 3786 1425 750 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 4316 4675 4834 518 Size: 648 1011 2139 359 159 2042 Fragments arranged by size: 2139 2042 1011 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2392 2495 2547 2572 2840 2954 3774 4451 Size: 786 103 52 25 268 114 820 677 Cuts at: 4451 5599 5844 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 820 786 677 268 245 114 103 52 25 NaeI GCC'GGC Cuts at: 2874 3225 3398 2874 Size: 351 173 5834 Fragments arranged by size: 5834 351 173 NarI GG'CG_CC Cuts at: 2026 2026 Size: 6358 NcoI C'CATG_G Cuts at: 513 1065 2203 2772 2978 513 Size: 552 1138 569 206 3893 Fragments arranged by size: 3893 1138 569 552 206 NdeI CA'TA_TG Cuts at: 387 387 Size: 6358 NgoMIV G'CCGG_C Cuts at: 2872 3223 3396 2872 Size: 351 173 5834 Fragments arranged by size: 5834 351 173 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6358 NotI GC'GGCC_GC Cuts at: 3805 3805 Size: 6358 NspI r_CATG'y Cuts at: 1132 1229 2305 3716 5261 1132 Size: 97 1076 1411 1545 2229 Fragments arranged by size: 2229 1545 1411 1076 97 PasI CC'CwG_GG Cuts at: 1348 2100 2754 2814 2850 3063 3236 1348 Size: 752 654 60 36 213 173 4470 Fragments arranged by size: 4470 752 654 213 173 60 36 PciI A'CATG_T Cuts at: 2301 5257 2301 Size: 2956 3402 PflMI CCAn_nnn'nTGG Cuts at: 1108 2338 3464 1108 Size: 1230 1126 4002 Fragments arranged by size: 4002 1230 1126 PfoI T'CCnGG_A Cuts at: 3179 3179 Size: 6358 PmeI GTTT'AAAC Cuts at: 3662 3662 Size: 6358 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4427 4459 5940 5972 4427 Size: 32 1481 32 4813 Fragments arranged by size: 4813 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2884 2912 2937 3001 2884 Size: 28 25 64 6241 Fragments arranged by size: 6241 64 28 25 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6358 PsiI TTA'TAA Cuts at: 3928 3928 Size: 6358 PspOMI G'GGCC_C Cuts at: 2104 2761 2963 3014 3356 3403 3592 3622 2104 Size: 657 202 51 342 47 189 30 4840 Fragments arranged by size: 4840 657 342 202 189 51 47 30 PstI C_TGCA'G Cuts at: 830 2565 2646 2911 2953 3304 3710 830 Size: 1735 81 265 42 351 406 3478 Fragments arranged by size: 3478 1735 406 351 265 81 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6358 PvuII CAG'CTG Cuts at: 2392 2495 2572 2840 2954 2392 Size: 103 77 268 114 5796 Fragments arranged by size: 5796 268 114 103 77 SalI G'TCGA_C Cuts at: 1936 2008 3374 3700 1936 Size: 72 1366 326 4594 Fragments arranged by size: 4594 1366 326 72 SanDI GG'GwC_CC Cuts at: 2884 2937 3001 2884 Size: 53 64 6241 Fragments arranged by size: 6241 64 53 SapI GCTCTTCn'nnn_ Cuts at: 2424 2424 Size: 6358 SbfI CC_TGCA'GG Cuts at: 2911 3710 2911 Size: 799 5559 ScaI AGT'ACT Cuts at: 4606 6318 4606 Size: 1712 4646 SfcI C'TryA_G Cuts at: 826 1045 2561 2642 2907 2949 3300 3706 4841 Size: 219 1516 81 265 42 351 406 1135 Cuts at: 4841 5522 5713 826 Size: 681 191 1471 Fragments arranged by size: 1516 1471 1135 681 406 351 265 219 191 81 42 SfoI GGC'GCC Cuts at: 2027 2027 Size: 6358 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6358 SmaI CCC'GGG Cuts at: 1872 3621 3685 1872 Size: 1749 64 4545 Fragments arranged by size: 4545 1749 64 SmlI C'TyrA_G Cuts at: 820 1017 1943 2020 2447 4462 5363 5625 820 Size: 197 926 77 427 2015 901 262 1553 Fragments arranged by size: 2015 1553 926 901 427 262 197 77 SnaBI TAC'GTA Cuts at: 493 4149 493 Size: 3656 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6358 SphI G_CATG'C Cuts at: 1132 3716 1132 Size: 2584 3774 SspI AAT'ATT Cuts at: 5 52 4282 5 Size: 47 4230 2081 Fragments arranged by size: 4230 2081 47 StuI AGG'CCT Cuts at: 1517 2896 3428 1517 Size: 1379 532 4447 Fragments arranged by size: 4447 1379 532 StyI C'CwwG_G Cuts at: 513 1065 2203 2454 2772 2927 2978 3423 3583 Size: 552 1138 251 318 155 51 445 160 Cuts at: 3583 3797 513 Size: 214 3074 Fragments arranged by size: 3074 1138 552 445 318 251 214 160 155 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2785 3168 3580 4385 4402 4555 4740 Size: 1255 1205 383 412 805 17 153 185 Cuts at: 4740 5185 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1205 805 445 412 383 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4604 6316 96 Size: 274 80 33 51 1443 2627 1712 138 Fragments arranged by size: 2627 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2516 2548 2959 2991 3298 3330 6318 6350 2516 Size: 32 411 32 307 32 2988 32 2524 Fragments arranged by size: 2988 2524 411 307 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2014 1316 Size: 698 5660 XbaI T'CTAG_A Cuts at: 3694 3694 Size: 6358 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2242 2461 2242 Size: 219 6139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6358 XmaI C'CCGG_G Cuts at: 1870 3619 3683 1870 Size: 1749 64 4545 Fragments arranged by size: 4545 1749 64 XmnI GAAnn'nnTTC Cuts at: 2327 4487 2327 Size: 2160 4198 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2716 4166 276 Size: 53 83 186 936 1182 1450 2468 Fragments arranged by size: 2468 1450 1182 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI