Restriction map for
pFN21AB5959
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# NO CUT ENZYMES ( 104 enzymes) #
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Acc65I AccI AccII AclI AcsI
AgeI AhdI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsiWI BsmI BspHI BspLU11I BsrDI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I DraI DraIII EagI Eam1105I
EclXI Eco52I EcoRI EcoRV FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tsp509I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 522 GACGTC GACGT^C
AccIII 1328 TCCGGA T^CCGGA
AflII 184 CTTAAG C^TTAAG
AflIII 897 ACACGT A^C[GA][CT]GT
AocI 1166 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1526 AGCGCT AGC^GCT
BalI 850 TGGCCA TGG^CCA
BfaI 112 CTAG C^TAG
BfrI 184 CTTAAG C^TTAAG
BlpI 1341 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1341 GCTAAGC GC^T[ACGT]AGC
BsaI 766 GAGACC GGTCTC(1/5)
BseAI 1328 TCCGGA T^CCGGA
BsiEI 1336 CGGTCG CG[GA][CT]^CG
BsmBI 444 CGTCTC CGTCTC(1/5)
BspDI 457 ATCGAT AT^CGAT
BspEI 1328 TCCGGA T^CCGGA
BsrBI 122 CCGCTC CCGCTC(-3/-3)
BstEII 309 GGTGACC G^GT[ACGT]ACC
BstPI 309 GGTGACC G^GT[ACGT]ACC
BstXI 716 CCAAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1250 AGATCC [GA]^GATC[CT]
Bsu36I 1166 CCTCAGG CC^T[ACGT]AGG
CelII 1341 GCTAAGC GC^T[ACGT]AGC
ClaI 457 ATCGAT AT^CGAT
CpoI 1325 CGGTCCG CG^G[AT]CCG
DrdI 522 GACGTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1097 CTCTTC CTCTTC(1/4)
Eco47III 1526 AGCGCT AGC^GCT
Eco81I 1166 CCTCAGG CC^T[ACGT]AGG
EcoNI 207 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 309 GGTGACC G^GT[ACGT]ACC
EcoT22I 1223 ATGCAT ATGCA^T
FauI 948 GCGGG CCCGC(4/6)
HaeII 1526 AGCGCT [GA]GCGC^[CT]
HindIII 1344 AAGCTT A^AGCTT
Ksp632I 1097 CTCTTC CTCTTC(1/4)
MaeI 112 CTAG C^TAG
MflI 1250 AGATCC [GA]^GATC[CT]
MluNI 850 TGGCCA TGG^CCA
MroI 1328 TCCGGA T^CCGGA
MscI 850 TGGCCA TGG^CCA
MseI 185 TTAA T^TAA
NsiI 1223 ATGCAT ATGCA^T
RsrII 1325 CGGTCCG CG^G[AT]CCG
SapI 1096 GCTCTTC GCTCTTC(1/4)
SmlI 184 CTTAAG C^T[CT][GA]AG
StuI 1517 AGGCCT AGG^CCT
TfiI 991 GAATC G^A[AT]TC
Tru9I 185 TTAA T^TAA
XcmI 747 CCAAACTGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1250 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 20 GACGCC G[GA]^CG[CT]C
522 GACGTC
Alw44I 267 GTGCAC G^TGCAC
1494 GTGCAC
AlwI 772 GATCC GGATC(4/5)
1251 GATCC
ApaLI 267 GTGCAC G^TGCAC
1494 GTGCAC
BclI 753 TGATCA T^GATCA
936 TGATCA
BsaHI 20 GACGCC G[GA]^CG[CT]C
522 GACGTC
BsaWI 1323 TCCGGT [AT]^CCGG[AT]
1328 TCCGGA
BseRI 358 GAGGAG GAGGAG(10/8)
952 GAGGAG
BsgI 1111 CTGCAC GTGCAG(16/14)
976 GTGCAG
Bsp1407I 546 TGTACA T^GTACA
836 TGTACA
BsrFI 1334 GCCGGT [GA]^CCGG[CT]
1505 GCCGGT
BsrGI 546 TGTACA T^GTACA
836 TGTACA
CfoI 873 GCGC GCG^C
1527 GCGC
Cfr10I 1334 GCCGGT [GA]^CCGG[CT]
1505 GCCGGT
DsaI 512 CCATGG C^C[GA][CT]GG
604 CCATGG
EaeI 509 CGGCCA [CT]^GGCC[GA]
850 TGGCCA
FbaI 753 TGATCA T^GATCA
936 TGATCA
HgaI 20 GACGC GACGC(5/10)
481 GACGC
HhaI 873 GCGC GCG^C
1527 GCGC
Hin1I 20 GACGCC G[GA]^CG[CT]C
522 GACGTC
HinP1I 873 GCGC G^CGC
1527 GCGC
MaeII 523 ACGT A^CGT
899 ACGT
MboII 1512 GAAGA GAAGA(8/7)
1098 TCTTC
MslI 658 CACTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
969 CACAGAGGTG
NcoI 512 CCATGG C^CATGG
604 CCATGG
PpuMI 60 GGGACCC [GA]G^G[AT]CC[CT]
1195 GGGTCCT
SspBI 546 TGTACA T^GTACA
836 TGTACA
TaqI 458 TCGA T^CGA
1475 TCGA
TthHB8I 458 TCGA T^CGA
1475 TCGA
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TseI Tsp45I TspRI
Van91I XmaCI XmaI