(Circular) (Six-base) MAPSORT of: pFN21AB5955.seq Check: 6095 from: 1 to: 6178 pFN21AB5955 6178 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3988 278 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3521 1937 Size: 1584 4594 Acc65I G'GTAC_C Cuts at: 3499 3499 Size: 6178 AclI AA'CG_TT Cuts at: 1563 3407 4305 4678 1563 Size: 1844 898 373 3063 Fragments arranged by size: 3063 1844 898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2792 2954 4241 5625 5893 1632 Size: 1160 162 1287 1384 268 1917 Fragments arranged by size: 1917 1384 1287 1160 268 162 AfeI AGC'GCT Cuts at: 2389 2389 Size: 6178 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5981 AflIII A'CryG_T Cuts at: 1203 5077 6054 1203 Size: 3874 977 1327 Fragments arranged by size: 3874 1327 977 AgeI A'CCGG_T Cuts at: 5009 5009 Size: 6178 AhdI GACnn_n'nnGTC Cuts at: 2185 4907 2185 Size: 2722 3456 AleI CACnn'nnGTG Cuts at: 1166 3079 1166 Size: 1913 4265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2975 1184 Size: 1791 4387 AlwNI CAG_nnn'CTG Cuts at: 3921 5493 3921 Size: 1572 4606 ApaLI G'TGCA_C Cuts at: 4235 5391 4235 Size: 1156 5022 ApoI r'AATT_y Cuts at: 1493 3264 3487 3713 3865 5974 1493 Size: 1771 223 226 152 2109 1697 Fragments arranged by size: 2109 1771 1697 226 223 152 AseI AT'TA_AT Cuts at: 160 1032 4732 160 Size: 872 3700 1606 Fragments arranged by size: 3700 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 AvaI C'yCGr_G Cuts at: 1870 1943 2670 3503 1870 Size: 73 727 833 4545 Fragments arranged by size: 4545 833 727 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2731 2764 2731 Size: 33 6145 BamHI G'GATC_C Cuts at: 3508 3875 3508 Size: 367 5811 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2374 3499 4954 618 Size: 325 217 627 587 1125 1455 1842 Fragments arranged by size: 1842 1455 1125 627 587 325 217 BanII G_rGCy'C Cuts at: 1391 2883 3357 3497 1391 Size: 1492 474 140 4072 Fragments arranged by size: 4072 1492 474 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2069 2831 928 Size: 971 170 762 4275 Fragments arranged by size: 4275 971 762 170 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4369 4403 1903 Size: 34 2432 34 3678 Fragments arranged by size: 3678 2432 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4070 5280 4070 Size: 1210 4968 BclI T'GATC_A Cuts at: 1548 1548 Size: 6178 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4789 136 Size: 107 122 71 631 605 3117 1525 Fragments arranged by size: 3117 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6173 6173 Size: 6178 BlpI GC'TnA_GC Cuts at: 3595 3595 Size: 6178 Bme1580I G_kGCm'C Cuts at: 1790 4239 5395 1790 Size: 2449 1156 2573 Fragments arranged by size: 2573 2449 1156 BmgBI CAC'GTC Cuts at: 1318 3295 1318 Size: 1977 4201 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3909 4867 442 Size: 416 53 83 399 24 2492 958 1753 Fragments arranged by size: 2492 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 2412 3159 4838 1239 Size: 1001 172 747 1679 2579 Fragments arranged by size: 2579 1679 1001 747 172 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2059 4303 5168 5466 2059 Size: 2244 865 298 2771 Fragments arranged by size: 2771 2244 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3070 3394 4841 882 Size: 617 1571 324 1447 2219 Fragments arranged by size: 2219 1571 1447 617 324 BsaAI yAC'GTr Cuts at: 493 1204 3969 493 Size: 711 2765 2702 Fragments arranged by size: 2765 2702 711 BsaBI GATnn'nnATC Cuts at: 6172 6172 Size: 6178 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3985 4367 5002 275 Size: 53 83 186 936 2452 382 635 1451 Fragments arranged by size: 2452 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4611 5009 5283 5430 5841 4611 Size: 398 274 147 411 4948 Fragments arranged by size: 4948 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2127 2157 1178 Size: 30 165 30 724 30 5199 Fragments arranged by size: 5199 724 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 2891 1186 Size: 1051 654 4473 Fragments arranged by size: 4473 1051 654 BseYI C'CCAG_C Cuts at: 2282 2705 5381 5877 2282 Size: 423 2676 496 2583 Fragments arranged by size: 2676 2583 496 423 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2617 1163 Size: 573 881 4724 Fragments arranged by size: 4724 881 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3628 4389 5417 1540 Size: 275 186 1627 761 1028 2301 Fragments arranged by size: 2301 1627 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3357 4239 4324 5395 3357 Size: 882 85 1071 4140 Fragments arranged by size: 4140 1071 882 85 BsmI GAATG_Cn' Cuts at: 3689 3782 3689 Size: 93 6085 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2883 3357 3497 4239 4324 5395 1391 Size: 399 1093 474 140 742 85 1071 2174 Fragments arranged by size: 2174 1093 1071 742 474 399 140 85 BspEI T'CCGG_A Cuts at: 5841 5841 Size: 6178 BspHI T'CATG_A Cuts at: 2820 4067 5797 2820 Size: 1247 1730 3201 Fragments arranged by size: 3201 1730 1247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3051 3533 844 Size: 2207 482 3489 Fragments arranged by size: 3489 2207 482 BsrBI CCG'CTC Cuts at: 1394 1823 3019 4065 1394 Size: 429 1196 1046 3507 Fragments arranged by size: 3507 1196 1046 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4673 4847 65 Size: 1183 3425 174 1396 Fragments arranged by size: 3425 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4822 5009 1700 Size: 110 3012 187 2869 Fragments arranged by size: 3012 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6178 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6178 BssSI C'ACGA_G Cuts at: 2846 4238 5250 2846 Size: 1392 1012 3774 Fragments arranged by size: 3774 1392 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6178 BstBI TT'CG_AA Cuts at: 4982 4982 Size: 6178 BstEII G'GTnAC_C Cuts at: 1184 2866 1184 Size: 1682 4496 BstXI CCAn_nnnn'nTGG Cuts at: 2686 2686 Size: 6178 BstYI r'GATC_y Cuts at: 1972 2119 2664 3508 3875 4260 4277 5729 5815 Size: 147 545 844 367 385 17 1452 86 Cuts at: 5815 5827 6173 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 844 545 385 367 346 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2642 3450 513 Size: 552 939 638 808 3241 Fragments arranged by size: 3241 939 808 638 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2955 487 Size: 683 56 1729 3710 Fragments arranged by size: 3710 1729 683 56 BtsI GCAGTG_nn' Cuts at: 3694 4488 4508 3694 Size: 794 20 5364 Fragments arranged by size: 5364 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6178 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6143 DraI TTT'AAA Cuts at: 3482 3482 Size: 6178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3307 5185 809 Size: 507 1991 1878 1802 Fragments arranged by size: 1991 1878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 2907 3625 Size: 54 1425 50 275 18 465 612 718 Cuts at: 3625 4514 6143 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 718 612 465 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3625 1537 Size: 275 1813 4090 Fragments arranged by size: 4090 1813 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2316 2930 3390 4108 1353 Size: 963 614 460 718 3423 Fragments arranged by size: 3423 963 718 614 460 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4765 5139 5285 387 Size: 1064 138 3176 374 146 1280 Fragments arranged by size: 3176 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3355 3355 Size: 6178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2412 2792 2954 3159 4241 4838 Size: 393 608 172 380 162 205 1082 597 Cuts at: 4838 5625 5893 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1082 787 608 597 393 380 268 205 172 162 EcoNI CCTnn'n_nnAGG Cuts at: 3311 3311 Size: 6178 EcoO109I rG'GnC_Cy Cuts at: 3040 3040 Size: 6178 EcoRI G'AATT_C Cuts at: 3487 3865 3487 Size: 378 5800 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2978 2978 Size: 6178 FspI TGC'GCA Cuts at: 4684 4684 Size: 6178 HaeII r_GCGC'y Cuts at: 1678 2391 2706 5325 1678 Size: 713 315 2619 2531 Fragments arranged by size: 2619 2531 713 315 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 3206 3238 4819 Size: 32 135 32 599 32 1003 32 1581 Cuts at: 4819 4851 4893 4925 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1581 1003 599 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3522 3768 669 Size: 988 281 1584 246 3079 Fragments arranged by size: 3079 1584 988 281 246 HindIII A'AGCT_T Cuts at: 748 2431 748 Size: 1683 4495 HpaI GTT'AAC Cuts at: 3768 3768 Size: 6178 KpnI G_GTAC'C Cuts at: 3503 3503 Size: 6178 MfeI C'AATT_G Cuts at: 3777 3777 Size: 6178 MluI A'CGCG_T Cuts at: 6054 6054 Size: 6178 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5292 5476 1305 Size: 3987 184 2007 Fragments arranged by size: 3987 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 2909 10 Size: 54 1425 343 465 612 3279 Fragments arranged by size: 3279 1425 612 465 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2472 3079 4136 4495 4654 518 Size: 648 1306 607 1057 359 159 2042 Fragments arranged by size: 2042 1306 1057 648 607 359 159 MspA1I CmG'CkG Cuts at: 1606 3594 4271 5419 5664 1606 Size: 1988 677 1148 245 2120 Fragments arranged by size: 2120 1988 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2642 513 Size: 552 939 638 4049 Fragments arranged by size: 4049 939 638 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6178 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6178 NotI GC'GGCC_GC Cuts at: 3625 3625 Size: 6178 NspI r_CATG'y Cuts at: 1132 1229 3536 5081 1132 Size: 97 2307 1545 2229 Fragments arranged by size: 2307 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3275 3313 1348 Size: 1927 38 4213 Fragments arranged by size: 4213 1927 38 PciI A'CATG_T Cuts at: 5077 5077 Size: 6178 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6178 PfoI T'CCnGG_A Cuts at: 2217 2802 3429 2217 Size: 585 627 4966 Fragments arranged by size: 4966 627 585 PmeI GTTT'AAAC Cuts at: 3482 3482 Size: 6178 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4247 4279 5760 5792 4247 Size: 32 1481 32 4633 Fragments arranged by size: 4633 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3040 3040 Size: 6178 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6178 PsiI TTA'TAA Cuts at: 3748 3748 Size: 6178 PstI C_TGCA'G Cuts at: 830 2835 3530 830 Size: 2005 695 3478 Fragments arranged by size: 3478 2005 695 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6178 SacI G_AGCT'C Cuts at: 3357 3357 Size: 6178 SalI G'TCGA_C Cuts at: 1936 3520 1936 Size: 1584 4594 SapI GCTCTTCn'nnn_ Cuts at: 2930 2930 Size: 6178 SbfI CC_TGCA'GG Cuts at: 3530 3530 Size: 6178 ScaI AGT'ACT Cuts at: 4426 6138 4426 Size: 1712 4466 SfcI C'TryA_G Cuts at: 826 1045 2831 3319 3526 4661 5342 5533 826 Size: 219 1786 488 207 1135 681 191 1471 Fragments arranged by size: 1786 1471 1135 681 488 219 207 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6178 SmaI CCC'GGG Cuts at: 1872 2672 3505 1872 Size: 800 833 4545 Fragments arranged by size: 4545 833 800 SmlI C'TyrA_G Cuts at: 820 1017 1943 2038 4282 5183 5445 820 Size: 197 926 95 2244 901 262 1553 Fragments arranged by size: 2244 1553 926 901 262 197 95 SnaBI TAC'GTA Cuts at: 493 3969 493 Size: 3476 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6178 SphI G_CATG'C Cuts at: 1132 3536 1132 Size: 2404 3774 SspI AAT'ATT Cuts at: 5 52 4102 5 Size: 47 4050 2081 Fragments arranged by size: 4050 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6178 StyI C'CwwG_G Cuts at: 513 1065 2004 2642 2751 2910 3617 513 Size: 552 939 638 109 159 707 3074 Fragments arranged by size: 3074 939 707 638 552 159 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4205 4222 4375 4560 5005 325 Size: 1255 2625 17 153 185 445 1498 Fragments arranged by size: 2625 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2901 3129 4424 Size: 274 80 33 51 1443 924 228 1295 Cuts at: 4424 6136 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1295 924 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2127 2159 3316 3348 6138 6170 2127 Size: 32 1157 32 2790 32 2135 Fragments arranged by size: 2790 2135 1157 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6178 XbaI T'CTAG_A Cuts at: 3514 3514 Size: 6178 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2290 2640 2290 Size: 350 5828 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6178 XmaI C'CCGG_G Cuts at: 1870 2670 3503 1870 Size: 800 833 4545 Fragments arranged by size: 4545 833 800 XmnI GAAnn'nnTTC Cuts at: 4307 4307 Size: 6178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3986 276 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI