Restriction map for
pFN21AB5940
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AclI AflIII AhdI
Alw44I AlwNI AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BciVI BclI BglI BlnI
BpmI BsaAI BsaBI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI BstAPI BstBI Bsu36I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MunI NheI NotI NruI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XcmI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 334 GTATAC GT^[AC][GT]AC
AflII 1018 CTTAAG C^TTAAG
AgeI 1301 ACCGGT A^CCGGT
AlwI 411 GATCC GGATC(4/5)
Aor51HI 144 AGCGCT AGC^GCT
ApaI 943 GGGCCC GGGCC^C
BalI 1515 TGGCCA TGG^CCA
BanI 117 GGCGCC G^G[CT][GA]CC
BbeI 117 GGCGCC GGCGC^C
BbsI 493 GAAGAC GAAGAC(2/6)
BcgI 1007 CGATTAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1018 CTTAAG C^TTAAG
BlpI 1345 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1345 GCTCAGC GC^T[ACGT]AGC
BpuAI 493 GAAGAC GAAGAC(2/6)
BsaI 950 GAGACC GGTCTC(1/5)
BsmAI 950 GAGAC GTCTC(1/5)
BspMI 461 ACCTGC ACCTGC(4/8)
BsrDI 1132 GCAATG GCAATG(2/0)
Bst1107I 334 GTATAC GTA^TAC
BstEII 999 GGTGACC G^GT[ACGT]ACC
BstPI 999 GGTGACC G^GT[ACGT]ACC
BstZ17I 334 GTATAC GTA^TAC
CelII 1345 GCTCAGC GC^T[ACGT]AGC
DraI 589 TTTAAA TTT^AAA
Eco47III 144 AGCGCT AGC^GCT
EcoNI 466 CCTCACATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 999 GGTGACC G^GT[ACGT]ACC
EcoRI 1119 GAATTC G^AATTC
EcoT14I 1312 CCATGG C^C[AT][AT]GG
EcoT22I 1051 ATGCAT ATGCA^T
HgaI 103 GACGC GACGC(5/10)
KasI 117 GGCGCC G^GCGCC
MaeII 991 ACGT A^CGT
MluNI 1515 TGGCCA TGG^CCA
MscI 1515 TGGCCA TGG^CCA
NaeI 120 GCCGGC GCC^GGC
NarI 117 GGCGCC GG^CGCC
NcoI 1312 CCATGG C^CATGG
NdeI 619 CATATG CA^TATG
NgoMIV 120 GCCGGC G^CCGGC
NsiI 1051 ATGCAT ATGCA^T
PaeR7I 389 CTCGAG C^TCGAG
PinAI 1301 ACCGGT A^CCGGT
PpuMI 166 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1275 GAGCTC GAGCT^C
SapI 976 GAAGAGC GCTCTTC(1/4)
SfaNI 1053 GCATC GCATC(5/9)
SfoI 117 GGCGCC GGC^GCC
SmaI 50 CCCGGG CCC^GGG
SphI 1028 GCATGC GCATG^C
StyI 1312 CCATGG C^C[AT][AT]GG
TaqI 390 TCGA T^CGA
TthHB8I 390 TCGA T^CGA
XbaI 1333 TCTAGA T^CTAGA
XhoI 389 CTCGAG C^TCGAG
XmaCI 50 CCCGGG C^CCGGG
XmaI 50 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 159 TCCGGA T^CCGGA
216 TCCGGA
AcyI 103 GACGCC G[GA]^CG[CT]C
117 GGCGCC
BanII 943 GGGCCC G[GA]GC[CT]^C
1275 GAGCTC
BcnI 50 CCCGG CC^[GC]GG
51 CCGGG
BfaI 1334 CTAG C^TAG
1607 CTAG
BsaHI 103 GACGCC G[GA]^CG[CT]C
117 GGCGCC
BseAI 159 TCCGGA T^CCGGA
216 TCCGGA
BsgI 133 CTGCAC GTGCAG(16/14)
968 GTGCAG
BsmFI 166 GGGAC GGGAC(10/14)
1305 GTCCC
BspEI 159 TCCGGA T^CCGGA
216 TCCGGA
BsrFI 120 GCCGGC [GA]^CCGG[CT]
1301 ACCGGT
BstXI 72 CCAGGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1283 CCACAAACATGG
Cfr10I 120 GCCGGC [GA]^CCGG[CT]
1301 ACCGGT
EaeI 65 TGGCCG [CT]^GGCC[GA]
1515 TGGCCA
EarI 1266 CTCTTC CTCTTC(1/4)
976 GAAGAG
FauI 170 CCCGC CCCGC(4/6)
937 GCGGG
Hin1I 103 GACGCC G[GA]^CG[CT]C
117 GGCGCC
Ksp632I 1266 CTCTTC CTCTTC(1/4)
976 GAAGAG
KspI 171 CCGCGG CCGC^GG
820 CCGCGG
MaeI 1334 CTAG C^TAG
1607 CTAG
MroI 159 TCCGGA T^CCGGA
216 TCCGGA
NciI 50 CCCGG CC^[GC]GG
51 CCGGG
NspI 723 ACATGC [GA]CATG^[CT]
1028 GCATGC
PleI 1208 GACTC GAGTC(4/5)
1437 GAGTC
PstI 182 CTGCAG CTGCA^G
1077 CTGCAG
SacII 171 CCGCGG CCGC^GG
820 CCGCGG
SfcI 182 CTGCAG C^T[GA][CT]AG
1077 CTGCAG
SmlI 389 CTCGAG C^T[CT][GA]AG
1018 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI AvaI AvaII BbvI
BglII BmyI BsaJI BsaWI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PflMI PvuII RsaI Sau3AI Sau96I
ScrFI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII