(Circular) (Six-base) MAPSORT of: pFN21AB5909.seq Check: 5400 from: 1 to: 6217 pFN21AB5909 6217 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2620 2620 Size: 6217 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 2117 3344 3538 2117 Size: 1227 194 4796 Fragments arranged by size: 4796 1227 194 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4280 5664 5932 1632 Size: 2648 1384 268 1917 Fragments arranged by size: 2648 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3275 820 Size: 197 2258 3762 Fragments arranged by size: 3762 2258 197 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 2049 2810 3032 3443 1166 Size: 883 761 222 411 3940 Fragments arranged by size: 3940 883 761 411 222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 2290 2836 3236 3960 5532 2290 Size: 546 400 724 1572 2975 Fragments arranged by size: 2975 1572 724 546 400 ApaI G_GGCC'C Cuts at: 2917 3185 2917 Size: 268 5949 ApaLI G'TGCA_C Cuts at: 3069 4274 5430 3069 Size: 1205 1156 3856 Fragments arranged by size: 3856 1205 1156 ApoI r'AATT_y Cuts at: 1493 3526 3752 3904 6013 1493 Size: 2033 226 152 2109 1697 Fragments arranged by size: 2109 2033 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2385 3178 3542 1870 Size: 73 442 793 364 4545 Fragments arranged by size: 4545 793 442 364 73 BamHI G'GATC_C Cuts at: 2673 3547 3914 2673 Size: 874 367 4976 Fragments arranged by size: 4976 874 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 3344 3538 4993 618 Size: 325 217 627 330 1227 194 1455 1842 Fragments arranged by size: 1842 1455 1227 627 330 325 217 194 BanII G_rGCy'C Cuts at: 1391 2616 2917 2975 3185 3536 1391 Size: 1225 301 58 210 351 4072 Fragments arranged by size: 4072 1225 351 301 210 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2585 928 Size: 971 686 4560 Fragments arranged by size: 4560 971 686 BbvCI CC'TCA_GC Cuts at: 2203 2939 2203 Size: 736 5481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2065 4109 5319 2065 Size: 2044 1210 2963 Fragments arranged by size: 2963 2044 1210 BclI T'GATC_A Cuts at: 1548 2983 1548 Size: 1435 4782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3180 4828 136 Size: 107 122 71 631 605 1508 1648 1525 Fragments arranged by size: 1648 1525 1508 631 605 122 107 71 BglII A'GATC_T Cuts at: 3129 3378 6212 3129 Size: 249 2834 3134 Fragments arranged by size: 3134 2834 249 BlpI GC'TnA_GC Cuts at: 2615 3634 2615 Size: 1019 5198 Bme1580I G_kGCm'C Cuts at: 1790 2433 2917 3073 3185 3368 4278 5434 1790 Size: 643 484 156 112 183 910 1156 2573 Fragments arranged by size: 2573 1156 910 643 484 183 156 112 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3001 3948 4906 Size: 416 53 83 399 24 1584 947 958 Cuts at: 4906 442 Size: 1753 Fragments arranged by size: 1753 1584 958 947 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4877 1239 Size: 3638 2579 Bpu10I CC'TnA_GC Cuts at: 2203 2939 2969 2203 Size: 736 30 5451 Fragments arranged by size: 5451 736 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3485 4342 5207 5505 3485 Size: 857 865 298 4197 Fragments arranged by size: 4197 865 857 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3038 4880 882 Size: 617 1539 1842 2219 Fragments arranged by size: 2219 1842 1539 617 BsaAI yAC'GTr Cuts at: 493 1204 2644 4008 493 Size: 711 1440 1364 2702 Fragments arranged by size: 2702 1440 1364 711 BsaBI GATnn'nnATC Cuts at: 3128 3383 6211 3128 Size: 255 2828 3134 Fragments arranged by size: 3134 2828 255 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4024 4406 5041 275 Size: 53 83 186 936 2491 382 635 1451 Fragments arranged by size: 2491 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4650 5048 5322 5469 5880 4650 Size: 398 274 147 411 4987 Fragments arranged by size: 4987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5992 Fragments arranged by size: 5992 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2606 2965 3235 1186 Size: 979 441 359 270 4168 Fragments arranged by size: 4168 979 441 359 270 BseYI C'CCAG_C Cuts at: 5420 5916 5420 Size: 496 5721 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2249 1163 Size: 573 513 5131 Fragments arranged by size: 5131 573 513 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2616 2975 3027 3073 4278 4363 5434 2046 Size: 570 359 52 46 1205 85 1071 2829 Fragments arranged by size: 2829 1205 1071 570 359 85 52 46 BsmI GAATG_Cn' Cuts at: 2664 3728 3821 2664 Size: 1064 93 5060 Fragments arranged by size: 5060 1064 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2366 2366 Size: 6217 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2433 2616 2917 2975 3027 3073 Size: 399 256 387 183 301 58 52 46 Cuts at: 3073 3185 3368 3536 4278 4363 5434 1391 Size: 112 183 168 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 301 256 183 183 168 112 85 58 52 46 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2620 3572 844 Size: 1776 952 3489 Fragments arranged by size: 3489 1776 952 BsrBI CCG'CTC Cuts at: 1394 1823 2083 4104 1394 Size: 429 260 2021 3507 Fragments arranged by size: 3507 2021 429 260 BsrDI GCAATG_nn' Cuts at: 65 1248 2103 2352 4712 4886 65 Size: 1183 855 249 2360 174 1396 Fragments arranged by size: 2360 1396 1183 855 249 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2625 4861 5048 1700 Size: 110 815 2236 187 2869 Fragments arranged by size: 2869 2236 815 187 110 BsrGI T'GTAC_A Cuts at: 96 2899 96 Size: 2803 3414 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6217 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 2803 1184 Size: 1619 4598 BstXI CCAn_nnnn'nTGG Cuts at: 3445 3445 Size: 6217 BstYI r'GATC_y Cuts at: 1972 2673 2712 3129 3262 3378 3547 3914 4299 Size: 701 39 417 133 116 169 367 385 Cuts at: 4299 4316 5768 5854 5866 6212 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 701 417 385 367 346 169 133 116 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 2589 2589 Size: 6217 BtgI C'CryG_G Cuts at: 513 1065 2556 3111 513 Size: 552 1491 555 3619 Fragments arranged by size: 3619 1491 555 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3181 3457 487 Size: 683 56 1955 276 3247 Fragments arranged by size: 3247 1955 683 276 56 BtsI GCAGTG_nn' Cuts at: 2376 3733 4527 4547 2376 Size: 1357 794 20 4046 Fragments arranged by size: 4046 1357 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2445 2480 529 Size: 35 1881 35 4266 Fragments arranged by size: 4266 1881 35 35 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3248 5224 809 Size: 507 1932 1976 1802 Fragments arranged by size: 1976 1932 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 3159 3172 Size: 54 1425 50 275 18 390 939 13 Cuts at: 3172 3664 4553 6182 8 Size: 492 889 1629 43 Fragments arranged by size: 1629 1425 939 889 492 390 275 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2564 2604 4147 1353 Size: 1211 40 1543 3423 Fragments arranged by size: 3423 1543 1211 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2744 4804 5178 5324 387 Size: 1064 138 1155 2060 374 146 1280 Fragments arranged by size: 2060 1280 1155 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2614 2973 2614 Size: 359 5858 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4280 4877 5664 5932 1239 Size: 393 2648 597 787 268 1524 Fragments arranged by size: 2648 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2833 3182 2833 Size: 349 5868 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FspI TGC'GCA Cuts at: 3269 4723 3269 Size: 1454 4763 HaeII r_GCGC'y Cuts at: 1678 5364 1678 Size: 3686 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3032 3064 3230 3262 3305 Size: 32 135 32 1460 32 166 32 43 Cuts at: 3305 3337 4858 4890 4932 4964 1373 Size: 32 1521 32 42 32 2626 Fragments arranged by size: 2626 1521 1460 166 135 43 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3561 3807 669 Size: 988 281 1623 246 3079 Fragments arranged by size: 3079 1623 988 281 246 HindIII A'AGCT_T Cuts at: 748 2232 2528 3272 748 Size: 1484 296 744 3693 Fragments arranged by size: 3693 1484 744 296 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 2121 3348 3542 2121 Size: 1227 194 4796 Fragments arranged by size: 4796 1227 194 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2724 5331 5515 1305 Size: 1419 2607 184 2007 Fragments arranged by size: 2607 2007 1419 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3161 3174 10 Size: 54 1425 343 1329 13 3053 Fragments arranged by size: 3053 1425 1329 343 54 13 MslI CAynn'nnrTG Cuts at: 518 1166 2049 2076 2810 2922 3032 3443 4175 Size: 648 883 27 734 112 110 411 732 Cuts at: 4175 4534 4693 518 Size: 359 159 2042 Fragments arranged by size: 2042 883 734 732 648 411 359 159 112 110 27 MspA1I CmG'CkG Cuts at: 1606 2290 3233 3298 3633 4310 5458 5703 1606 Size: 684 943 65 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 943 684 677 335 245 65 NcoI C'CATG_G Cuts at: 513 1065 3111 513 Size: 552 2046 3619 Fragments arranged by size: 3619 2046 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 3575 5120 1132 Size: 97 2346 1545 2229 Fragments arranged by size: 2346 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2417 2836 1108 Size: 1309 419 4489 Fragments arranged by size: 4489 1309 419 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PmlI CAC'GTG Cuts at: 2644 2644 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2833 2833 Size: 6217 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspOMI G'GGCC_C Cuts at: 2913 3181 2913 Size: 268 5949 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2892 2924 2892 Size: 32 6185 PstI C_TGCA'G Cuts at: 830 2295 3569 830 Size: 1465 1274 3478 Fragments arranged by size: 3478 1465 1274 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2290 3298 2290 Size: 1008 5209 SacI G_AGCT'C Cuts at: 2616 2975 2616 Size: 359 5858 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SapI GCTCTTCn'nnn_ Cuts at: 2604 2604 Size: 6217 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 2990 4465 6177 2990 Size: 1475 1712 3030 Fragments arranged by size: 3030 1712 1475 SexAI A'CCwGG_T Cuts at: 2835 2835 Size: 6217 SfcI C'TryA_G Cuts at: 826 1045 2291 3228 3508 3565 4700 5381 5572 Size: 219 1246 937 280 57 1135 681 191 Cuts at: 5572 826 Size: 1471 Fragments arranged by size: 1471 1246 1135 937 681 280 219 191 57 SfiI GGCCn_nnn'nGGCC Cuts at: 3180 3180 Size: 6217 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3275 3500 4321 5222 5484 820 Size: 197 926 1332 225 821 901 262 1553 Fragments arranged by size: 1553 1332 926 901 821 262 225 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 2937 1517 Size: 1420 4797 StyI C'CwwG_G Cuts at: 513 1065 2367 3030 3111 3656 513 Size: 552 1302 663 81 545 3074 Fragments arranged by size: 3074 1302 663 552 545 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2061 2196 2456 2480 2869 4244 4261 Size: 1255 481 135 260 24 389 1375 17 Cuts at: 4261 4414 4599 5044 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1375 1255 481 445 389 260 185 153 135 24 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2100 2899 2988 Size: 274 80 33 51 1443 123 799 89 Cuts at: 2988 4463 6175 96 Size: 1475 1712 138 Fragments arranged by size: 1712 1475 1443 799 274 138 123 89 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6177 6209 6177 Size: 32 6185 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 4346 4346 Size: 6217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI RsrII SacII SanDI SfoI SrfI SwaI XcmI