Restriction map for
pFN21AB5908
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmI BspEI BspLU11I BsrBI
BsrDI BssHII Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tsp509I
Tth111I Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 1090 CGCG CG^CG
AflII 349 CTTAAG C^TTAAG
AgeI 510 ACCGGT A^CCGGT
Asp700I 1255 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 899 TGGCCA TGG^CCA
BbsI 1426 GAAGAC GAAGAC(2/6)
BfrI 349 CTTAAG C^TTAAG
BpuAI 1426 GAAGAC GAAGAC(2/6)
BsaI 1213 GGTCTC GGTCTC(1/5)
BsaWI 510 ACCGGT [AT]^CCGG[AT]
BsgI 355 GTGCAG GTGCAG(16/14)
BsmBI 1543 GAGACG CGTCTC(1/5)
Bsp1407I 1312 TGTACA T^GTACA
BspDI 679 ATCGAT AT^CGAT
BspHI 51 TCATGA T^CATGA
BsrGI 1312 TGTACA T^GTACA
BssSI 647 CACGAG CACGAG(-5/-1)
BstBI 31 TTCGAA TT^CGAA
BstUI 1090 CGCG CG^CG
BstXI 1403 CCACAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 679 ATCGAT AT^CGAT
DraII 124 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 899 TGGCCA [CT]^GGCC[GA]
EcoO109I 124 GGGGCCT [GA]G^G[ACGT]CC[CT]
HgaI 652 GCGTC GACGC(5/10)
HincII 86 GTCAAC GT[CT]^[GA]AC
HindII 86 GTCAAC GT[CT]^[GA]AC
MaeIII 1465 GTCAC ^GT[ACGT]AC
MluNI 899 TGGCCA TGG^CCA
MscI 899 TGGCCA TGG^CCA
MspA1I 91 CCGCTG C[AC]G^C[GT]G
MvnI 1090 CGCG CG^CG
NcoI 542 CCATGG C^CATGG
NspV 31 TTCGAA TT^CGAA
PaeR7I 1178 CTCGAG C^TCGAG
PinAI 510 ACCGGT A^CCGGT
RcaI 51 TCATGA T^CATGA
SfuI 31 TTCGAA TT^CGAA
SgrAI 509 CACCGGTG C[GA]^CCGG[CT]G
SspBI 1312 TGTACA T^GTACA
StuI 819 AGGCCT AGG^CCT
Tsp45I 1465 GTCAC ^GT[GC]AC
XhoI 1178 CTCGAG C^TCGAG
XmnI 1255 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 232 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
878 CAGAGTCTG
AvaI 771 CTCGGG C^[CT]CG[GA]G
1178 CTCGAG
BamHI 735 GGATCC G^GATCC
1414 GGATCC
BcnI 517 CCGGG CC^[GC]GG
1495 CCCGG
BfaI 697 CTAG C^TAG
982 CTAG
BsoBI 771 CTCGGG C^[CT]CG[GA]G
1178 CTCGAG
BspMI 330 GCAGGT ACCTGC(4/8)
411 GCAGGT
BsrFI 510 ACCGGT [GA]^CCGG[CT]
1136 ACCGGC
BstYI 735 GGATCC [GA]^GATC[CT]
1414 GGATCC
Cfr10I 510 ACCGGT [GA]^CCGG[CT]
1136 ACCGGC
Cfr13I 125 GGGCC G^G[ACGT]CC
246 GGCCC
EcoT14I 542 CCATGG C^C[AT][AT]GG
801 CCAAGG
MaeI 697 CTAG C^TAG
982 CTAG
MaeII 296 ACGT A^CGT
760 ACGT
MflI 735 GGATCC [GA]^GATC[CT]
1414 GGATCC
NciI 517 CCGGG CC^[GC]GG
1495 CCCGG
NspI 450 ACATGC [GA]CATG^[CT]
1016 ACATGC
PleI 276 GAGTC GAGTC(4/5)
880 GAGTC
PstI 238 CTGCAG CTGCA^G
1272 CTGCAG
SacI 871 GAGCTC GAGCT^C
1181 GAGCTC
Sau96I 125 GGGCC G^G[ACGT]CC
246 GGCCC
StyI 542 CCATGG C^C[AT][AT]GG
801 CCAAGG
XcmI 533 CCAACAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1012 CCAGACATGCGCTGG
XhoII 735 GGATCC [GA]^GATC[CT]
1414 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanI BanII BbvI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI Cac8I CfoI
DdeI DpnII DsaI EarI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MboI MboII MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI SapI Sau3AI
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI TspRI TthHB8I