(Circular) (Six-base) MAPSORT of: pFN21AB5908.seq Check: 625 from: 1 to: 6265 pFN21AB5908 6265 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2326 2326 Size: 6265 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 2910 3278 4328 5712 5980 1632 Size: 488 790 368 1050 1384 268 1917 Fragments arranged by size: 1917 1384 1050 790 488 368 268 AflII C'TTAA_G Cuts at: 820 1017 2354 820 Size: 197 1337 4731 Fragments arranged by size: 4731 1337 197 AflIII A'CryG_T Cuts at: 1203 5164 6141 1203 Size: 3961 977 1327 Fragments arranged by size: 3961 1327 977 AgeI A'CCGG_T Cuts at: 2515 5096 2515 Size: 2581 3684 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 2336 3561 1166 Size: 1170 1225 3870 Fragments arranged by size: 3870 1225 1170 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 2242 2888 4008 5580 2242 Size: 646 1120 1572 2927 Fragments arranged by size: 2927 1572 1120 646 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 3574 3800 3952 6061 1493 Size: 2081 226 152 2109 1697 Fragments arranged by size: 2109 2081 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2776 3183 3590 1870 Size: 73 833 407 407 4545 Fragments arranged by size: 4545 833 407 407 73 BamHI G'GATC_C Cuts at: 2740 3419 3595 3962 2740 Size: 679 176 367 5043 Fragments arranged by size: 5043 679 367 176 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2338 2518 3344 3586 5041 Size: 325 217 627 551 180 826 242 1455 Cuts at: 5041 618 Size: 1842 Fragments arranged by size: 1842 1455 826 627 551 325 242 217 180 BanII G_rGCy'C Cuts at: 1391 2067 2880 3190 3584 1391 Size: 676 813 310 394 4072 Fragments arranged by size: 4072 813 676 394 310 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3438 928 Size: 971 1539 3755 Fragments arranged by size: 3755 1539 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4456 4490 1903 Size: 34 50 34 2435 34 3678 Fragments arranged by size: 3678 2435 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4876 136 Size: 107 122 71 631 605 3204 1525 Fragments arranged by size: 3204 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 2277 2313 2633 4326 5482 1790 Size: 487 36 320 1693 1156 2573 Fragments arranged by size: 2573 1693 1156 487 320 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2248 3384 3996 Size: 416 53 83 399 24 831 1136 612 Cuts at: 3996 4954 442 Size: 958 1753 Fragments arranged by size: 1753 1136 958 831 612 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2140 3149 3213 4925 1239 Size: 901 1009 64 1712 2579 Fragments arranged by size: 2579 1712 1009 901 64 Bpu10I CC'TnA_GC Cuts at: 2471 2471 Size: 6265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3290 4390 5255 5553 3290 Size: 1100 865 298 4002 Fragments arranged by size: 4002 1100 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3224 4928 882 Size: 617 1725 1704 2219 Fragments arranged by size: 2219 1725 1704 617 BsaAI yAC'GTr Cuts at: 493 1204 4056 493 Size: 711 2852 2702 Fragments arranged by size: 2852 2702 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4072 4454 5089 275 Size: 53 83 186 936 2539 382 635 1451 Fragments arranged by size: 2539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2515 4698 5096 5370 5517 5928 2515 Size: 2183 398 274 147 411 2852 Fragments arranged by size: 2852 2183 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3442 3472 3517 3547 1178 Size: 30 165 30 2039 30 45 30 3896 Fragments arranged by size: 3896 2039 165 45 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2143 3541 1186 Size: 877 80 1398 3910 Fragments arranged by size: 3910 1398 877 80 BseYI C'CCAG_C Cuts at: 5468 5964 5468 Size: 496 5769 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 1163 Size: 573 645 5047 Fragments arranged by size: 5047 645 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2880 2902 3135 3190 3558 4326 4411 5482 2880 Size: 22 233 55 368 768 85 1071 3663 Fragments arranged by size: 3663 1071 768 368 233 85 55 22 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 BsmBI CGTCTCn'nnnn_ Cuts at: 3542 3542 Size: 6265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2277 2313 2633 2880 2902 3135 Size: 399 277 210 36 320 247 22 233 Cuts at: 3135 3190 3558 3584 4326 4411 5482 1391 Size: 55 368 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 368 320 277 247 233 210 85 55 36 26 22 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 2056 4154 5884 2056 Size: 2098 1730 2437 Fragments arranged by size: 2437 2098 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2326 2407 3620 844 Size: 1482 81 1213 3489 Fragments arranged by size: 3489 1482 1213 81 BsrBI CCG'CTC Cuts at: 1394 1823 4152 1394 Size: 429 2329 3507 Fragments arranged by size: 3507 2329 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 4934 65 Size: 1183 3512 174 1396 Fragments arranged by size: 3512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2515 3141 4909 5096 1700 Size: 110 705 626 1768 187 2869 Fragments arranged by size: 2869 1768 705 626 187 110 BsrGI T'GTAC_A Cuts at: 96 3317 96 Size: 3221 3044 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 2652 4325 5337 2652 Size: 1673 1012 3580 Fragments arranged by size: 3580 1673 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2194 2242 3151 794 Size: 1400 48 909 3908 Fragments arranged by size: 3908 1400 909 48 BstBI TT'CG_AA Cuts at: 2037 5069 2037 Size: 3032 3233 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstXI CCAn_nnnn'nTGG Cuts at: 3415 3415 Size: 6265 BstYI r'GATC_y Cuts at: 1972 2740 3419 3595 3962 4347 4364 5816 5902 Size: 768 679 176 367 385 17 1452 86 Cuts at: 5902 5914 6260 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 768 679 385 367 346 176 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2547 3057 3213 513 Size: 552 1482 510 156 3565 Fragments arranged by size: 3565 1482 552 510 156 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 3781 4575 4595 3781 Size: 794 20 5451 Fragments arranged by size: 5451 794 20 ClaI AT'CG_AT Cuts at: 1554 2685 1554 Size: 1131 5134 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2904 3712 4601 Size: 54 1425 50 275 18 1074 808 889 Cuts at: 4601 6230 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1074 889 808 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2055 2096 2598 3540 4195 1353 Size: 702 41 502 942 655 3423 Fragments arranged by size: 3423 942 702 655 502 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2104 4852 5226 5372 387 Size: 1064 138 515 2748 374 146 1280 Fragments arranged by size: 2748 1280 1064 515 374 146 138 EcoICRI GAG'CTC Cuts at: 2878 3188 2878 Size: 310 5955 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2140 2910 3149 3213 3278 4328 Size: 393 488 20 770 239 64 65 1050 Cuts at: 4328 4925 5712 5980 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1050 787 770 597 488 393 268 239 65 64 20 EcoO109I rG'GnC_Cy Cuts at: 2130 2130 Size: 6265 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3308 3308 Size: 6265 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 5412 1678 Size: 3734 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4906 4938 4980 5012 1373 Size: 32 135 32 3334 32 42 32 2626 Fragments arranged by size: 3334 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2093 3609 3855 669 Size: 988 281 155 1516 246 3079 Fragments arranged by size: 3079 1516 988 281 246 155 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2595 5379 5563 1305 Size: 1290 2784 184 2007 Fragments arranged by size: 2784 2007 1290 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2906 10 Size: 54 1425 343 1074 3369 Fragments arranged by size: 3369 1425 1074 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2336 2552 3413 3440 3561 4223 4582 Size: 648 1170 216 861 27 121 662 359 Cuts at: 4582 4741 518 Size: 159 2042 Fragments arranged by size: 2042 1170 861 662 648 359 216 159 121 27 MspA1I CmG'CkG Cuts at: 1606 2098 3681 4358 5506 5751 1606 Size: 492 1583 677 1148 245 2120 Fragments arranged by size: 2120 1583 1148 677 492 245 NcoI C'CATG_G Cuts at: 513 1065 2547 513 Size: 552 1482 4231 Fragments arranged by size: 4231 1482 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2459 3025 3623 5168 1132 Size: 97 1230 566 598 1545 2229 Fragments arranged by size: 2229 1545 1230 598 566 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6265 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 2127 4334 4366 5847 5879 2095 Size: 32 2207 32 1481 32 2481 Fragments arranged by size: 2481 2207 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 2595 2715 3835 2595 Size: 120 1120 5025 Fragments arranged by size: 5025 1120 120 PspXI vC'TCGA_Gb Cuts at: 3183 3183 Size: 6265 PstI C_TGCA'G Cuts at: 830 2247 3281 3617 830 Size: 1417 1034 336 3478 Fragments arranged by size: 3478 1417 1034 336 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 SacI G_AGCT'C Cuts at: 2880 3190 2880 Size: 310 5955 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SapI GCTCTTCn'nnn_ Cuts at: 2055 2096 2598 2055 Size: 41 502 5722 Fragments arranged by size: 5722 502 41 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SfcI C'TryA_G Cuts at: 826 1045 2243 3277 3397 3613 4748 5429 5620 Size: 219 1198 1034 120 216 1135 681 191 Cuts at: 5620 826 Size: 1471 Fragments arranged by size: 1471 1198 1135 1034 681 219 216 191 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 2515 1700 Size: 815 5450 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2354 3183 3269 4369 5270 5532 Size: 197 926 411 829 86 1100 901 262 Cuts at: 5532 820 Size: 1553 Fragments arranged by size: 1553 1100 926 901 829 411 262 197 86 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 2826 1517 Size: 1309 4956 StyI C'CwwG_G Cuts at: 513 1065 2547 2806 3704 513 Size: 552 1482 259 898 3074 Fragments arranged by size: 3074 1482 898 552 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2551 4292 4309 4462 4647 5092 325 Size: 1255 971 1741 17 153 185 445 1498 Fragments arranged by size: 1741 1498 1255 971 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3317 4511 6223 Size: 274 80 33 51 1443 1340 1194 1712 Cuts at: 6223 96 Size: 138 Fragments arranged by size: 1712 1443 1340 1194 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2305 2337 3442 3474 6225 6257 2305 Size: 32 1105 32 2751 32 2313 Fragments arranged by size: 2751 2313 1105 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2545 3024 2545 Size: 479 5786 XhoI C'TCGA_G Cuts at: 1943 3183 1943 Size: 1240 5025 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 3264 4394 3264 Size: 1130 5135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI