Restriction map for
pFN21AB5908
(Linear) (Six-base) MAPSORT of: KIBB5908.seq Check: 8430 from: 1 to: 1560
KIBB5908.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 321 1560
Size: 321 1239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 905 1273 1560
Size: 115 790 368 287
Fragments arranged by size:
790 368 287 115
AflII C'TTAA_G
Cuts at: 0 349 1560
Size: 349 1211
AgeI A'CCGG_T
Cuts at: 0 510 1560
Size: 510 1050
AleI CACnn'nnGTG
Cuts at: 0 331 1560
Size: 331 1229
AlwNI CAG_nnn'CTG
Cuts at: 0 237 883 1560
Size: 237 646 677
Fragments arranged by size:
677 646 237
AvaI C'yCGr_G
Cuts at: 0 771 1178 1560
Size: 771 407 382
Fragments arranged by size:
771 407 382
BamHI G'GATC_C
Cuts at: 0 735 1414 1560
Size: 735 679 146
Fragments arranged by size:
735 679 146
BanI G'GyrC_C
Cuts at: 0 333 513 1339 1560
Size: 333 180 826 221
Fragments arranged by size:
826 333 221 180
BanII G_rGCy'C
Cuts at: 0 62 875 1185 1560
Size: 62 813 310 375
Fragments arranged by size:
813 375 310 62
BbsI GAAGACnn'nnnn_
Cuts at: 0 1433 1560
Size: 1433 127
Bme1580I G_kGCm'C
Cuts at: 0 272 308 628 1560
Size: 272 36 320 932
Fragments arranged by size:
932 320 272 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 243 1379 1560
Size: 243 1136 181
Fragments arranged by size:
1136 243 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 1144 1208 1560
Size: 135 1009 64 352
Fragments arranged by size:
1009 352 135 64
Bpu10I CC'TnA_GC
Cuts at: 0 466 1560
Size: 466 1094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1285 1560
Size: 1285 275
BsaI GGTCTCn'nnnn_
Cuts at: 0 1219 1560
Size: 1219 341
BsaWI w'CCGG_w
Cuts at: 0 510 1560
Size: 510 1050
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1437 1467 1512 1542 1560
Size: 1437 30 45 30 18
Fragments arranged by size:
1437 45 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 138 1536 1560
Size: 58 80 1398 24
Fragments arranged by size:
1398 80 58 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1560
Size: 376 1184
BsiHKAI G_wGCw'C
Cuts at: 0 875 897 1130 1185 1553 1560
Size: 875 22 233 55 368 7
Fragments arranged by size:
875 368 233 55 22 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1537 1560
Size: 1537 23
Bsp1286I G_dGCh'C
Cuts at: 0 62 272 308 628 875 897 1130 1185
Size: 62 210 36 320 247 22 233 55
Cuts at: 1185 1553 1560
Size: 368 7
Fragments arranged by size:
368 320 247 233 210 62 55 36
22 7
BspHI T'CATG_A
Cuts at: 0 51 1560
Size: 51 1509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 321 402 1560
Size: 321 81 1158
Fragments arranged by size:
1158 321 81
BsrFI r'CCGG_y
Cuts at: 0 510 1136 1560
Size: 510 626 424
Fragments arranged by size:
626 510 424
BsrGI T'GTAC_A
Cuts at: 0 1312 1560
Size: 1312 248
BssSI C'ACGA_G
Cuts at: 0 647 1560
Size: 647 913
BstAPI GCAn_nnn'nTGC
Cuts at: 0 189 237 1146 1560
Size: 189 48 909 414
Fragments arranged by size:
909 414 189 48
BstBI TT'CG_AA
Cuts at: 0 32 1560
Size: 32 1528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1410 1560
Size: 1410 150
BstYI r'GATC_y
Cuts at: 0 735 1414 1560
Size: 735 679 146
Fragments arranged by size:
735 679 146
BtgI C'CryG_G
Cuts at: 0 542 1052 1208 1560
Size: 542 510 156 352
Fragments arranged by size:
542 510 352 156
ClaI AT'CG_AT
Cuts at: 0 680 1560
Size: 680 880
EaeI y'GGCC_r
Cuts at: 0 899 1560
Size: 899 661
EarI CTCTTCn'nnn_
Cuts at: 0 50 91 593 1535 1560
Size: 50 41 502 942 25
Fragments arranged by size:
942 502 50 41 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 99 1560
Size: 99 1461
EcoICRI GAG'CTC
Cuts at: 0 873 1183 1560
Size: 873 310 377
Fragments arranged by size:
873 377 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 135 905 1144 1208 1273 1560
Size: 115 20 770 239 64 65 287
Fragments arranged by size:
770 287 239 115 65 64 20
EcoO109I rG'GnC_Cy
Cuts at: 0 125 1560
Size: 125 1435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1303 1560
Size: 1303 257
HincII GTy'rAC
Cuts at: 0 88 1560
Size: 88 1472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 1560
Size: 590 970
MscI TGG'CCA
Cuts at: 0 901 1560
Size: 901 659
MslI CAynn'nnrTG
Cuts at: 0 331 547 1408 1435 1560
Size: 331 216 861 27 125
Fragments arranged by size:
861 331 216 125 27
MspA1I CmG'CkG
Cuts at: 0 93 1560
Size: 93 1467
NcoI C'CATG_G
Cuts at: 0 542 1560
Size: 542 1018
NspI r_CATG'y
Cuts at: 0 454 1020 1560
Size: 454 566 540
Fragments arranged by size:
566 540 454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 122 1560
Size: 90 32 1438
Fragments arranged by size:
1438 90 32
PsiI TTA'TAA
Cuts at: 0 590 710 1560
Size: 590 120 850
Fragments arranged by size:
850 590 120
PspXI vC'TCGA_Gb
Cuts at: 0 1178 1560
Size: 1178 382
PstI C_TGCA'G
Cuts at: 0 242 1276 1560
Size: 242 1034 284
Fragments arranged by size:
1034 284 242
SacI G_AGCT'C
Cuts at: 0 875 1185 1560
Size: 875 310 375
Fragments arranged by size:
875 375 310
SapI GCTCTTCn'nnn_
Cuts at: 0 50 91 593 1560
Size: 50 41 502 967
Fragments arranged by size:
967 502 50 41
SfcI C'TryA_G
Cuts at: 0 238 1272 1392 1560
Size: 238 1034 120 168
Fragments arranged by size:
1034 238 168 120
SgrAI Cr'CCGG_yG
Cuts at: 0 510 1560
Size: 510 1050
SmlI C'TyrA_G
Cuts at: 0 349 1178 1264 1560
Size: 349 829 86 296
Fragments arranged by size:
829 349 296 86
StuI AGG'CCT
Cuts at: 0 821 1560
Size: 821 739
StyI C'CwwG_G
Cuts at: 0 542 801 1560
Size: 542 259 759
Fragments arranged by size:
759 542 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 546 1560
Size: 546 1014
TatI w'GTAC_w
Cuts at: 0 1312 1560
Size: 1312 248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 300 332 1437 1469 1560
Size: 300 32 1105 32 91
Fragments arranged by size:
1105 300 91 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 540 1019 1560
Size: 540 479 541
Fragments arranged by size:
541 540 479
XhoI C'TCGA_G
Cuts at: 0 1178 1560
Size: 1178 382
XmnI GAAnn'nnTTC
Cuts at: 0 1259 1560
Size: 1259 301
Enzymes that do cut:
AarI AcuI AflII AgeI AleI AlwNI AvaI BamHI
BanI BanII BbsI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI ClaI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I FalI HincII MmeI MscI MslI MspA1I NcoI
NspI PpiI PsiI PspXI PstI SacI SapI SfcI
SgrAI SmlI StuI StyI TaqII TatI TstI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI
BseYI BsiEI BsiWI BsmI BspEI BsrBI BsrDI BssHII
BstEII BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII
DrdI EagI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI
PshAI PspOMI PsrI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XmaI ZraI