(Circular) (Six-base) MAPSORT of: pFN21AB5893.seq Check: 3166 from: 1 to: 6202 pFN21AB5893 6202 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3234 4012 278 Size: 53 83 186 936 1698 778 2468 Fragments arranged by size: 2468 1698 936 778 186 83 53 AccI GT'mk_AC Cuts at: 1937 3545 1937 Size: 1608 4594 Acc65I G'GTAC_C Cuts at: 3523 3523 Size: 6202 AclI AA'CG_TT Cuts at: 1563 4329 4702 1563 Size: 2766 373 3063 Fragments arranged by size: 3063 2766 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 4265 5649 5917 1632 Size: 467 2166 1384 268 1917 Fragments arranged by size: 2166 1917 1384 467 268 AfeI AGC'GCT Cuts at: 2480 2480 Size: 6202 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6005 AflIII A'CryG_T Cuts at: 1203 5101 6078 1203 Size: 3898 977 1327 Fragments arranged by size: 3898 1327 977 AgeI A'CCGG_T Cuts at: 5033 5033 Size: 6202 AhdI GACnn_n'nnGTC Cuts at: 4931 4931 Size: 6202 AleI CACnn'nnGTG Cuts at: 1166 2681 3437 1166 Size: 1515 756 3931 Fragments arranged by size: 3931 1515 756 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6202 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3365 3397 3365 Size: 32 6170 AlwNI CAG_nnn'CTG Cuts at: 2641 3945 5517 2641 Size: 1304 1572 3326 Fragments arranged by size: 3326 1572 1304 ApaI G_GGCC'C Cuts at: 3144 3144 Size: 6202 ApaLI G'TGCA_C Cuts at: 4259 5415 4259 Size: 1156 5046 ApoI r'AATT_y Cuts at: 1493 3511 3737 3889 5998 1493 Size: 2018 226 152 2109 1697 Fragments arranged by size: 2109 2018 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4756 160 Size: 872 3724 1606 Fragments arranged by size: 3724 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 AvaI C'yCGr_G Cuts at: 1870 1943 2049 3527 1870 Size: 73 106 1478 4545 Fragments arranged by size: 4545 1478 106 73 BamHI G'GATC_C Cuts at: 3532 3899 3532 Size: 367 5835 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3523 4978 618 Size: 325 217 627 1736 1455 1842 Fragments arranged by size: 1842 1736 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2046 2077 2097 2586 2772 2994 3144 3404 Size: 655 31 20 489 186 222 150 260 Cuts at: 3404 3521 1391 Size: 117 4072 Fragments arranged by size: 4072 655 489 260 222 186 150 117 31 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2556 3084 3331 928 Size: 971 657 528 247 3799 Fragments arranged by size: 3799 971 657 528 247 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2410 2444 4393 4427 1903 Size: 34 473 34 1949 34 3678 Fragments arranged by size: 3678 1949 473 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4094 5304 4094 Size: 1210 4992 BclI T'GATC_A Cuts at: 1548 3051 1548 Size: 1503 4699 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2264 4813 136 Size: 107 122 71 631 605 592 2549 1525 Fragments arranged by size: 2549 1525 631 605 592 122 107 71 BglII A'GATC_T Cuts at: 2937 6197 2937 Size: 3260 2942 BlpI GC'TnA_GC Cuts at: 2040 2672 3619 2040 Size: 632 947 4623 Fragments arranged by size: 4623 947 632 Bme1580I G_kGCm'C Cuts at: 1790 3144 4263 5419 1790 Size: 1354 1119 1156 2573 Fragments arranged by size: 2573 1354 1156 1119 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2339 3022 3933 Size: 416 53 83 399 24 922 683 911 Cuts at: 3933 4891 442 Size: 958 1753 Fragments arranged by size: 1753 958 922 911 683 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6202 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2680 2680 Size: 6202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3380 4862 1239 Size: 2141 1482 2579 Fragments arranged by size: 2579 2141 1482 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2081 4327 5192 5490 2081 Size: 2246 865 298 2793 Fragments arranged by size: 2793 2246 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2783 4865 882 Size: 617 1284 2082 2219 Fragments arranged by size: 2219 2082 1284 617 BsaAI yAC'GTr Cuts at: 493 1204 3993 493 Size: 711 2789 2702 Fragments arranged by size: 2789 2702 711 BsaBI GATnn'nnATC Cuts at: 6196 6196 Size: 6202 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2256 3231 4009 4391 Size: 53 83 186 936 723 975 778 382 Cuts at: 4391 5026 275 Size: 635 1451 Fragments arranged by size: 1451 975 936 778 723 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2619 3102 4635 5033 5307 5454 5865 2619 Size: 483 1533 398 274 147 411 2956 Fragments arranged by size: 2956 1533 483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2465 2495 2816 2846 3365 Size: 30 165 30 1062 30 321 30 519 Cuts at: 3365 3395 1178 Size: 30 3985 Fragments arranged by size: 3985 1062 519 321 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2744 1186 Size: 919 639 4644 Fragments arranged by size: 4644 919 639 BseYI C'CCAG_C Cuts at: 2180 2248 5405 5901 2180 Size: 68 3157 496 2481 Fragments arranged by size: 3157 2481 496 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2600 1163 Size: 573 864 4765 Fragments arranged by size: 4765 864 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3652 4413 5441 1540 Size: 275 186 1651 761 1028 2301 Fragments arranged by size: 2301 1651 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2077 2097 2475 2586 2678 2772 2994 4263 4348 Size: 20 378 111 92 94 222 1269 85 Cuts at: 4348 5419 2077 Size: 1071 2860 Fragments arranged by size: 2860 1269 1071 378 222 111 94 92 85 20 BsmI GAATG_Cn' Cuts at: 3713 3806 3713 Size: 93 6109 BsmBI CGTCTCn'nnnn_ Cuts at: 2247 2357 2247 Size: 110 6092 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2077 2097 2475 2586 2678 2772 Size: 399 256 31 20 378 111 92 94 Cuts at: 2772 2994 3144 3404 3521 4263 4348 5419 1391 Size: 222 150 260 117 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 378 260 256 222 150 117 111 94 92 85 31 20 BspEI T'CCGG_A Cuts at: 3102 5865 3102 Size: 2763 3439 BspHI T'CATG_A Cuts at: 2574 4091 5821 2574 Size: 1517 1730 2955 Fragments arranged by size: 2955 1730 1517 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2229 3162 3557 844 Size: 1385 933 395 3489 Fragments arranged by size: 3489 1385 933 395 BsrBI CCG'CTC Cuts at: 1394 1823 2452 3115 4089 1394 Size: 429 629 663 974 3507 Fragments arranged by size: 3507 974 663 629 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2191 4697 4871 65 Size: 1183 943 2506 174 1396 Fragments arranged by size: 2506 1396 1183 943 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4846 5033 1700 Size: 110 3036 187 2869 Fragments arranged by size: 3036 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6202 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6202 BssSI C'ACGA_G Cuts at: 2681 2915 4262 5274 2681 Size: 234 1347 1012 3609 Fragments arranged by size: 3609 1347 1012 234 BstAPI GCAn_nnn'nTGC Cuts at: 794 2232 794 Size: 1438 4764 BstBI TT'CG_AA Cuts at: 5006 5006 Size: 6202 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6202 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2275 2323 2011 Size: 264 48 5890 Fragments arranged by size: 5890 264 48 BstYI r'GATC_y Cuts at: 1972 2937 3166 3532 3899 4284 4301 5753 5839 Size: 965 229 366 367 385 17 1452 86 Cuts at: 5839 5851 6197 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 965 385 367 366 346 229 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2484 2734 3462 513 Size: 552 939 480 250 728 3253 Fragments arranged by size: 3253 939 728 552 480 250 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2024 2443 487 Size: 683 56 798 419 4246 Fragments arranged by size: 4246 798 683 419 56 BtsI GCAGTG_nn' Cuts at: 2597 3444 3718 4512 4532 2597 Size: 847 274 794 20 4267 Fragments arranged by size: 4267 847 794 274 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6167 DraI TTT'AAA Cuts at: 3506 3506 Size: 6202 DraIII CAC_nnn'GTG Cuts at: 2232 2232 Size: 6202 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5209 809 Size: 507 3893 1802 Fragments arranged by size: 3893 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2737 3649 4538 Size: 54 1425 50 275 18 907 912 889 Cuts at: 4538 6167 8 Size: 1629 43 Fragments arranged by size: 1629 1425 912 907 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3649 1537 Size: 275 1837 4090 Fragments arranged by size: 4090 1837 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3246 4132 1353 Size: 1893 886 3423 Fragments arranged by size: 3423 1893 886 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 4789 5163 5309 387 Size: 1064 138 441 2759 374 146 1280 Fragments arranged by size: 2759 1280 1064 441 374 146 138 EcoICRI GAG'CTC Cuts at: 2075 2095 2584 2770 2992 2075 Size: 20 489 186 222 5285 Fragments arranged by size: 5285 489 222 186 20 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 3380 4265 4862 5649 5917 1239 Size: 393 467 1281 885 597 787 268 1524 Fragments arranged by size: 1524 1281 885 787 597 467 393 268 EcoO109I rG'GnC_Cy Cuts at: 2659 3140 2659 Size: 481 5721 EcoRI G'AATT_C Cuts at: 3511 3889 3511 Size: 378 5824 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3262 3262 Size: 6202 FspI TGC'GCA Cuts at: 2225 4708 2225 Size: 2483 3719 HaeII r_GCGC'y Cuts at: 1678 2482 3190 5349 1678 Size: 804 708 2159 2531 Fragments arranged by size: 2531 2159 804 708 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2816 2848 4843 4875 4917 Size: 32 135 32 1244 32 1995 32 42 Cuts at: 4917 4949 1373 Size: 32 2626 Fragments arranged by size: 2626 1995 1244 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3546 3792 669 Size: 988 281 1608 246 3079 Fragments arranged by size: 3079 1608 988 281 246 HindIII A'AGCT_T Cuts at: 748 2543 748 Size: 1795 4407 HpaI GTT'AAC Cuts at: 3792 3792 Size: 6202 KpnI G_GTAC'C Cuts at: 3527 3527 Size: 6202 MfeI C'AATT_G Cuts at: 3801 3801 Size: 6202 MluI A'CGCG_T Cuts at: 6078 6078 Size: 6202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5316 5500 1305 Size: 4011 184 2007 Fragments arranged by size: 4011 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2739 10 Size: 54 1425 343 907 3473 Fragments arranged by size: 3473 1425 907 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2681 3437 4160 4519 4678 518 Size: 648 843 672 756 723 359 159 2042 Fragments arranged by size: 2042 843 756 723 672 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2535 3327 3618 4295 5443 5688 1606 Size: 929 792 291 677 1148 245 2120 Fragments arranged by size: 2120 1148 929 792 677 291 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2734 3462 513 Size: 552 939 730 728 3253 Fragments arranged by size: 3253 939 730 728 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6202 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6202 NotI GC'GGCC_GC Cuts at: 3649 3649 Size: 6202 NspI r_CATG'y Cuts at: 1132 1229 3560 5105 1132 Size: 97 2331 1545 2229 Fragments arranged by size: 2331 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6202 PciI A'CATG_T Cuts at: 5101 5101 Size: 6202 PflMI CCAn_nnn'nTGG Cuts at: 1108 2641 1108 Size: 1533 4669 PmeI GTTT'AAAC Cuts at: 3506 3506 Size: 6202 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3173 3205 3365 3397 4271 4303 5784 5816 3173 Size: 32 160 32 874 32 1481 32 3559 Fragments arranged by size: 3559 1481 874 160 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2659 2659 Size: 6202 PshAI GACnn'nnGTC Cuts at: 1613 2369 1613 Size: 756 5446 PsiI TTA'TAA Cuts at: 3772 3772 Size: 6202 PspOMI G'GGCC_C Cuts at: 3140 3140 Size: 6202 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2924 2956 2924 Size: 32 6170 PstI C_TGCA'G Cuts at: 830 3489 3554 830 Size: 2659 65 3478 Fragments arranged by size: 3478 2659 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6202 SacI G_AGCT'C Cuts at: 2077 2097 2586 2772 2994 2077 Size: 20 489 186 222 5285 Fragments arranged by size: 5285 489 222 186 20 SalI G'TCGA_C Cuts at: 1936 3544 1936 Size: 1608 4594 SbfI CC_TGCA'GG Cuts at: 3554 3554 Size: 6202 ScaI AGT'ACT Cuts at: 4450 6162 4450 Size: 1712 4490 SfcI C'TryA_G Cuts at: 826 1045 2155 2841 3485 3550 4685 5366 5557 Size: 219 1110 686 644 65 1135 681 191 Cuts at: 5557 826 Size: 1471 Fragments arranged by size: 1471 1135 1110 686 681 644 219 191 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6202 SmaI CCC'GGG Cuts at: 1872 2051 3529 1872 Size: 179 1478 4545 Fragments arranged by size: 4545 1478 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 4306 5207 5469 820 Size: 197 926 153 2210 901 262 1553 Fragments arranged by size: 2210 1553 926 901 262 197 153 SnaBI TAC'GTA Cuts at: 493 3993 493 Size: 3500 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6202 SphI G_CATG'C Cuts at: 1132 3560 1132 Size: 2428 3774 SspI AAT'ATT Cuts at: 5 52 4126 5 Size: 47 4074 2081 Fragments arranged by size: 4074 2081 47 StuI AGG'CCT Cuts at: 1517 2444 3066 3242 1517 Size: 927 622 176 4477 Fragments arranged by size: 4477 927 622 176 StyI C'CwwG_G Cuts at: 513 1065 2004 2127 2734 3237 3462 3641 513 Size: 552 939 123 607 503 225 179 3074 Fragments arranged by size: 3074 939 607 552 503 225 179 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2747 2841 4229 4246 4399 4584 5029 Size: 1255 1167 94 1388 17 153 185 445 Cuts at: 5029 325 Size: 1498 Fragments arranged by size: 1498 1388 1255 1167 445 185 153 94 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2286 2529 2970 Size: 274 80 33 51 1443 309 243 441 Cuts at: 2970 4448 6160 96 Size: 1478 1712 138 Fragments arranged by size: 1712 1478 1443 441 309 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2020 2052 2465 2497 6162 6194 2020 Size: 32 413 32 3665 32 2028 Fragments arranged by size: 3665 2028 413 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3083 1316 Size: 1767 4435 XbaI T'CTAG_A Cuts at: 3538 3538 Size: 6202 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3244 3244 Size: 6202 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6202 XmaI C'CCGG_G Cuts at: 1870 2049 3527 1870 Size: 179 1478 4545 Fragments arranged by size: 4545 1478 179 XmnI GAAnn'nnTTC Cuts at: 4331 4331 Size: 6202 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3232 4010 276 Size: 53 83 186 936 1698 778 2468 Fragments arranged by size: 2468 1698 936 778 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI