Restriction map for
pFN21AB5865
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AvrII BanI BbeI BbrPI
BciVI BfrI BglI BglII BlnI
BsaBI BsaI BsaWI BseAI BsiEI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoT22I
FauI FseI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspBI SwaI
Tru9I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 991 CGCG CG^CG
AcsI 409 AAATTC [GA]^AATT[CT]
AcyI 1204 GGCGTC G[GA]^CG[CT]C
Alw44I 398 GTGCAC G^TGCAC
AocI 945 CCTGAGG CC^T[ACGT]AGG
ApaLI 398 GTGCAC G^TGCAC
ApoI 409 AAATTC [GA]^AATT[CT]
Asp700I 1026 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1012 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 654 TGCGCA TGC^GCA
BamHI 262 GGATCC G^GATCC
BbvCI 463 GCTGAGG CCTCAGC(-5/-2)
BcnI 1224 CCGGG CC^[GC]GG
BfaI 26 CTAG C^TAG
BlpI 1048 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1048 GCTGAGC GC^T[ACGT]AGC
BsaAI 268 CACGTA [CT]AC^GT[GA]
BsaHI 1204 GGCGTC G[GA]^CG[CT]C
BsgI 958 GTGCAG GTGCAG(16/14)
BsiWI 270 CGTACG C^GTACG
BsmAI 535 GAGAC GTCTC(1/5)
BsrBI 844 CCGCTC CCGCTC(-3/-3)
BssSI 721 CTCGTG CACGAG(-5/-1)
BstUI 991 CGCG CG^CG
BstXI 1111 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 945 CCTGAGG CC^T[ACGT]AGG
CelII 1048 GCTGAGC GC^T[ACGT]AGC
DsaI 833 CCACGG C^C[GA][CT]GG
Eco81I 945 CCTGAGG CC^T[ACGT]AGG
EcoRV 358 GATATC GAT^ATC
EcoT14I 773 CCAAGG C^C[AT][AT]GG
FspI 654 TGCGCA TGC^GCA
HapII 1224 CCGG C^CGG
Hin1I 1204 GGCGTC G[GA]^CG[CT]C
HincII 1357 GTCAAC GT[CT]^[GA]AC
HindII 1357 GTCAAC GT[CT]^[GA]AC
HinfI 279 GAATC G^A[ACGT]TC
HpaII 1224 CCGG C^CGG
MaeI 26 CTAG C^TAG
MaeII 269 ACGT A^CGT
MslI 972 CACGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 910 CAGCGG C[AC]G^C[GT]G
MspI 1224 CCGG C^CGG
MvnI 991 CGCG CG^CG
NciI 1224 CCGGG CC^[GC]GG
PflFI 1012 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SmlI 391 CTCAAG C^T[CT][GA]AG
SphI 44 GCATGC GCATG^C
SspI 629 AATATT AAT^ATT
StuI 1113 AGGCCT AGG^CCT
StyI 773 CCAAGG C^C[AT][AT]GG
TaqI 794 TCGA T^CGA
TfiI 279 GAATC G^A[AT]TC
Tth111I 1012 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 794 TCGA T^CGA
XmnI 1026 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaI 928 CTCGGG C^[CT]CG[GA]G
1165 CCCGAG
BbsI 445 GAAGAC GAAGAC(2/6)
680 GTCTTC
BcgI 1167 CGAGCATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1083 GCAGCAGTATCG
BclI 563 TGATCA T^GATCA
692 TGATCA
BpuAI 445 GAAGAC GAAGAC(2/6)
680 GTCTTC
BsoBI 928 CTCGGG C^[CT]CG[GA]G
1165 CCCGAG
BstAPI 657 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
810 GCAGAAGCTGC
BstYI 262 GGATCC [GA]^GATC[CT]
387 GGATCT
EcoNI 720 CCTCGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
954 CCTGGTGCAGG
FbaI 563 TGATCA T^GATCA
692 TGATCA
HaeII 751 GGCGCT [GA]GCGC^[CT]
1158 GGCGCT
HgaI 216 GACGC GACGC(5/10)
1205 GCGTC
MaeIII 997 GTCAC ^GT[ACGT]AC
1018 GTCAC
MflI 262 GGATCC [GA]^GATC[CT]
387 GGATCT
NspI 44 GCATGC [GA]CATG^[CT]
542 ACATGC
SapI 132 GAAGAGC GCTCTTC(1/4)
477 GCTCTTC
SbfI 256 CCTGCAGG CCTGCA^GG
1272 CCTGCAGG
SexAI 1013 ACCTGGT A^CC[AT]GGT
1286 ACCAGGT
SfaNI 187 GATGC GCATC(5/9)
1174 GATGC
Sse8387I 256 CCTGCAGG CCTGCA^GG
1272 CCTGCAGG
Tsp45I 997 GTCAC ^GT[GC]AC
1018 GTCAC
Tsp509I 337 AATT ^AATT
410 AATT
XcmI 584 CCAGCTCCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
773 CCAAGGTCCAGGTGG
XhoII 262 GGATCC [GA]^GATC[CT]
387 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanII BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HphI
ItaI Ksp632I MboI MboII MluNI
MnlI MscI MvaI MwoI NdeII
NlaIII NlaIV PflMI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfcI
TseI TspRI Van91I