(Circular) (Six-base) MAPSORT of: pFN21AB5865.seq Check: 8416 from: 1 to: 6088 pFN21AB5865 6088 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3233 3233 Size: 6088 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3431 1937 Size: 1494 4594 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2129 2225 2273 2525 2948 3395 4151 5535 Size: 497 96 48 252 423 447 756 1384 Cuts at: 5535 5803 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 756 497 447 423 268 252 96 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 4987 5964 1203 Size: 3784 977 1327 Fragments arranged by size: 3784 1327 977 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 2981 1166 Size: 1815 4273 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2999 1184 Size: 1815 4273 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3020 3052 3020 Size: 32 6056 AlwNI CAG_nnn'CTG Cuts at: 2020 2107 2668 2821 2926 2950 3205 3298 3349 Size: 87 561 153 105 24 255 93 51 Cuts at: 3349 3831 5403 2020 Size: 482 1572 2705 Fragments arranged by size: 2705 1572 561 482 255 153 105 93 87 51 24 ApaLI G'TGCA_C Cuts at: 2403 4145 5301 2403 Size: 1742 1156 3190 Fragments arranged by size: 3190 1742 1156 ApoI r'AATT_y Cuts at: 1493 2414 3397 3623 3775 5884 1493 Size: 921 983 226 152 2109 1697 Fragments arranged by size: 2109 1697 983 921 226 152 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 2933 3170 3413 1870 Size: 73 990 237 243 4545 Fragments arranged by size: 4545 990 243 237 73 BamHI G'GATC_C Cuts at: 2267 3418 3785 2267 Size: 1151 367 4570 Fragments arranged by size: 4570 1151 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3409 4864 618 Size: 325 217 627 1622 1455 1842 Fragments arranged by size: 1842 1622 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2724 2812 3359 3407 1391 Size: 1333 88 547 48 4072 Fragments arranged by size: 4072 1333 547 88 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2457 2678 928 Size: 971 558 221 4338 Fragments arranged by size: 4338 971 558 221 BbvCI CC'TCA_GC Cuts at: 2469 2469 Size: 6088 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3077 3111 3161 3195 4279 4313 1903 Size: 34 1140 34 50 34 1084 34 3678 Fragments arranged by size: 3678 1140 1084 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 2568 2697 1548 Size: 1020 129 4939 Fragments arranged by size: 4939 1020 129 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4699 136 Size: 107 122 71 631 605 3027 1525 Fragments arranged by size: 3027 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 3054 3505 3054 Size: 451 5637 Bme1580I G_kGCm'C Cuts at: 1790 2407 2978 4149 5305 1790 Size: 617 571 1171 1156 2573 Fragments arranged by size: 2573 1171 1156 617 571 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2304 3215 3819 Size: 416 53 83 399 24 887 911 604 Cuts at: 3819 4777 442 Size: 958 1753 Fragments arranged by size: 1753 958 911 887 604 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2578 3329 4748 1239 Size: 986 353 751 1419 2579 Fragments arranged by size: 2579 1419 986 751 353 Bpu10I CC'TnA_GC Cuts at: 2469 2469 Size: 6088 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2381 4213 5078 5376 2381 Size: 1832 865 298 3093 Fragments arranged by size: 3093 1832 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2275 3879 493 Size: 711 1071 1604 2702 Fragments arranged by size: 2702 1604 1071 711 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3210 3895 4277 4912 Size: 53 83 186 936 1677 685 382 635 Cuts at: 4912 275 Size: 1451 Fragments arranged by size: 1677 1451 936 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4521 4919 5193 5340 5751 4521 Size: 398 274 147 411 4858 Fragments arranged by size: 4858 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3020 3050 1178 Size: 30 165 30 1617 30 4216 Fragments arranged by size: 4216 1617 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2031 2195 2198 3059 3062 1186 Size: 845 164 3 861 3 4212 Fragments arranged by size: 4212 861 845 164 3 3 BseYI C'CCAG_C Cuts at: 2752 2913 3252 3352 5291 5787 2752 Size: 161 339 100 1939 496 3053 Fragments arranged by size: 3053 1939 496 339 161 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2984 1163 Size: 573 1248 4267 Fragments arranged by size: 4267 1248 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2407 2888 2988 4149 4234 5305 2407 Size: 481 100 1161 85 1071 3190 Fragments arranged by size: 3190 1161 1071 481 100 85 BsiWI C'GTAC_G Cuts at: 2275 2275 Size: 6088 BsmI GAATG_Cn' Cuts at: 3599 3692 3599 Size: 93 5995 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2407 2724 2812 2888 2978 2988 3359 Size: 399 617 317 88 76 90 10 371 Cuts at: 3359 3407 4149 4234 5305 1391 Size: 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 617 399 371 317 90 88 85 76 48 10 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2676 2935 3233 3443 844 Size: 1832 259 298 210 3489 Fragments arranged by size: 3489 1832 298 259 210 BsrBI CCG'CTC Cuts at: 1394 1823 2851 3975 1394 Size: 429 1028 1124 3507 Fragments arranged by size: 3507 1124 1028 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4583 4757 65 Size: 1183 3335 174 1396 Fragments arranged by size: 3335 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4732 4919 1700 Size: 110 2922 187 2869 Fragments arranged by size: 2922 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 2726 4148 5160 2726 Size: 1422 1012 3654 Fragments arranged by size: 3654 1422 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2668 2821 794 Size: 1874 153 4061 Fragments arranged by size: 4061 1874 153 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstXI CCAn_nnnn'nTGG Cuts at: 3123 3123 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2267 2392 3418 3785 4170 4187 5639 5725 Size: 295 125 1026 367 385 17 1452 86 Cuts at: 5725 5737 6083 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1026 385 367 346 295 125 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2951 2951 Size: 6088 BtgI C'CryG_G Cuts at: 513 1065 2004 2838 513 Size: 552 939 834 3763 Fragments arranged by size: 3763 939 834 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 2853 3005 3604 4398 4418 2853 Size: 152 599 794 20 4523 Fragments arranged by size: 4523 794 599 152 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2835 3270 Size: 54 1425 50 275 18 240 765 435 Cuts at: 3270 3300 3535 4424 6053 8 Size: 30 235 889 1629 43 Fragments arranged by size: 1629 1425 889 765 435 275 240 235 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2132 2489 2598 4018 1353 Size: 779 357 109 1420 3423 Fragments arranged by size: 3423 1420 779 357 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2477 4675 5049 5195 387 Size: 1064 138 888 2198 374 146 1280 Fragments arranged by size: 2198 1280 1064 888 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2225 2273 2525 2578 2948 3329 Size: 393 497 96 48 252 53 370 381 Cuts at: 3329 3395 4151 4748 5535 5803 1239 Size: 66 756 597 787 268 1524 Fragments arranged by size: 1524 787 756 597 497 393 381 370 268 252 96 66 53 48 EcoNI CCTnn'n_nnAGG Cuts at: 2729 2963 2729 Size: 234 5854 EcoO109I rG'GnC_Cy Cuts at: 2236 2665 2869 2947 2955 3247 2236 Size: 429 204 78 8 292 5077 Fragments arranged by size: 5077 429 292 204 78 8 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 EcoRV GAT'ATC Cuts at: 2365 2365 Size: 6088 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2500 2530 2500 Size: 30 6058 FspI TGC'GCA Cuts at: 2661 4594 2661 Size: 1933 4155 HaeII r_GCGC'y Cuts at: 1678 2760 3167 5235 1678 Size: 1082 407 2068 2531 Fragments arranged by size: 2531 2068 1082 407 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 4729 4761 4803 Size: 32 135 32 454 32 2671 32 42 Cuts at: 4803 4835 1373 Size: 32 2626 Fragments arranged by size: 2671 2626 454 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3364 3432 3678 669 Size: 988 281 1426 68 246 3079 Fragments arranged by size: 3079 1426 988 281 246 68 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2837 3272 3302 10 Size: 54 1425 343 1005 435 30 2796 Fragments arranged by size: 2796 1425 1005 435 343 54 30 MslI CAynn'nnrTG Cuts at: 518 1166 2981 4046 4405 4564 518 Size: 648 1815 1065 359 159 2042 Fragments arranged by size: 2042 1815 1065 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2917 3504 4181 5329 5574 1606 Size: 1311 587 677 1148 245 2120 Fragments arranged by size: 2120 1311 1148 677 587 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4597 Fragments arranged by size: 4597 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 2053 2551 3446 4991 1132 Size: 97 824 498 895 1545 2229 Fragments arranged by size: 2229 1545 895 824 498 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 2650 3160 3298 1108 Size: 1542 510 138 3898 Fragments arranged by size: 3898 1542 510 138 PfoI T'CCnGG_A Cuts at: 2202 3342 2202 Size: 1140 4948 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2315 2347 3020 3052 4157 4189 5670 5702 2315 Size: 32 673 32 1105 32 1481 32 2701 Fragments arranged by size: 2701 1481 1105 673 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2665 2869 2947 2955 3247 2665 Size: 204 78 8 292 5506 Fragments arranged by size: 5506 292 204 78 8 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PstI C_TGCA'G Cuts at: 830 2266 2817 2946 3090 3201 3282 3318 3440 Size: 1436 551 129 144 111 81 36 122 Cuts at: 3440 830 Size: 3478 Fragments arranged by size: 3478 1436 551 144 129 122 111 81 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SapI GCTCTTCn'nnn_ Cuts at: 2132 2489 2132 Size: 357 5731 SbfI CC_TGCA'GG Cuts at: 2266 3282 3440 2266 Size: 1016 158 4914 Fragments arranged by size: 4914 1016 158 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SexAI A'CCwGG_T Cuts at: 3018 3291 3018 Size: 273 5815 SfcI C'TryA_G Cuts at: 826 1045 2262 2813 2942 3086 3197 3278 3314 Size: 219 1217 551 129 144 111 81 36 Cuts at: 3314 3436 4571 5252 5443 826 Size: 122 1135 681 191 1471 Fragments arranged by size: 1471 1217 1135 681 551 219 191 144 129 122 111 81 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2396 4192 5093 5355 820 Size: 197 926 453 1796 901 262 1553 Fragments arranged by size: 1796 1553 926 901 453 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 2053 3446 1132 Size: 921 1393 3774 Fragments arranged by size: 3774 1393 921 SspI AAT'ATT Cuts at: 5 52 2636 4012 5 Size: 47 2584 1376 2081 Fragments arranged by size: 2584 2081 1376 47 StuI AGG'CCT Cuts at: 1517 3120 1517 Size: 1603 4485 StyI C'CwwG_G Cuts at: 513 1065 2004 2778 3527 513 Size: 552 939 774 749 3074 Fragments arranged by size: 3074 939 774 749 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4115 4132 4285 4470 4915 325 Size: 1255 2535 17 153 185 445 1498 Fragments arranged by size: 2535 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4334 6046 96 Size: 274 80 33 51 1443 2357 1712 138 Fragments arranged by size: 2357 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3195 3227 6048 6080 3195 Size: 32 2821 32 3203 Fragments arranged by size: 3203 2821 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3020 1316 Size: 1704 4384 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2596 2785 2596 Size: 189 5899 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 3035 4217 3035 Size: 1182 4906 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI