(Circular) (Six-base) MAPSORT of: pFN21AB5815.seq Check: 9736 from: 1 to: 6118 pFN21AB5815 6118 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3461 1937 Size: 1524 4594 Acc65I G'GTAC_C Cuts at: 3439 3439 Size: 6118 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4181 5565 5833 1632 Size: 2549 1384 268 1917 Fragments arranged by size: 2549 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 5017 5994 1203 Size: 3814 977 1327 Fragments arranged by size: 3814 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 3305 1166 Size: 2139 3979 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6118 AlwNI CAG_nnn'CTG Cuts at: 2360 3337 3861 5433 2360 Size: 977 524 1572 3045 Fragments arranged by size: 3045 1572 977 524 ApaI G_GGCC'C Cuts at: 2856 2856 Size: 6118 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2590 3427 3653 3805 5914 1493 Size: 1097 837 226 152 2109 1697 Fragments arranged by size: 2109 1697 1097 837 226 152 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 2169 3443 1870 Size: 73 226 1274 4545 Fragments arranged by size: 4545 1274 226 73 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2413 2451 3268 3439 4894 Size: 325 217 627 626 38 817 171 1455 Cuts at: 4894 618 Size: 1842 Fragments arranged by size: 1842 1455 817 627 626 325 217 171 38 BanII G_rGCy'C Cuts at: 1391 2856 2898 3089 3437 1391 Size: 1465 42 191 348 4072 Fragments arranged by size: 4072 1465 348 191 42 BbeI G_GCGC'C Cuts at: 2455 2455 Size: 6118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4010 5220 4010 Size: 1210 4908 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3342 4729 136 Size: 107 122 71 631 605 1670 1387 1525 Fragments arranged by size: 1670 1525 1387 631 605 122 107 71 BglII A'GATC_T Cuts at: 3285 6113 3285 Size: 2828 3290 BlpI GC'TnA_GC Cuts at: 3035 3535 3035 Size: 500 5618 Bme1580I G_kGCm'C Cuts at: 1790 2418 2856 3273 4179 5335 1790 Size: 628 438 417 906 1156 2573 Fragments arranged by size: 2573 1156 906 628 438 417 BmgBI CAC'GTC Cuts at: 1318 2284 1318 Size: 966 5152 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2287 2552 3849 Size: 416 53 83 399 24 870 265 1297 Cuts at: 3849 4807 442 Size: 958 1753 Fragments arranged by size: 1753 1297 958 870 416 399 265 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2629 2746 3319 2629 Size: 117 573 5428 Fragments arranged by size: 5428 573 117 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4778 1239 Size: 3539 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2843 4243 5108 5406 2843 Size: 1400 865 298 3555 Fragments arranged by size: 3555 1400 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4781 882 Size: 617 3282 2219 Fragments arranged by size: 3282 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2461 2926 3909 493 Size: 711 1257 465 983 2702 Fragments arranged by size: 2702 1257 983 711 465 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2452 3053 3261 3925 Size: 53 83 186 936 919 601 208 664 Cuts at: 3925 4307 4942 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 919 664 635 601 382 208 186 83 53 BsaWI w'CCGG_w Cuts at: 4551 4949 5223 5370 5781 4551 Size: 398 274 147 411 4888 Fragments arranged by size: 4888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5893 Fragments arranged by size: 5893 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2504 3167 3185 3194 1186 Size: 1045 273 663 18 9 4110 Fragments arranged by size: 4110 1045 663 273 18 9 BseYI C'CCAG_C Cuts at: 2977 5321 5817 2977 Size: 2344 496 3278 Fragments arranged by size: 3278 2344 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2591 3116 3229 1163 Size: 573 855 525 113 4052 Fragments arranged by size: 4052 855 573 525 113 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2140 3568 4329 5357 1540 Size: 275 186 139 1428 761 1028 2301 Fragments arranged by size: 2301 1428 1028 761 275 186 139 BsiHKAI G_wGCw'C Cuts at: 2223 2307 3041 3089 4179 4264 5335 2223 Size: 84 734 48 1090 85 1071 3006 Fragments arranged by size: 3006 1090 1071 734 85 84 48 BsmI GAATG_Cn' Cuts at: 3031 3629 3722 3031 Size: 598 93 5427 Fragments arranged by size: 5427 598 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2307 2418 2856 2898 3041 3089 Size: 399 433 84 111 438 42 143 48 Cuts at: 3089 3273 3437 4179 4264 5335 1391 Size: 184 164 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 438 433 399 184 164 143 111 85 84 48 42 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 3132 4007 5737 3132 Size: 875 1730 3513 Fragments arranged by size: 3513 1730 875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3473 844 Size: 2629 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2029 2056 2097 2613 4005 1394 Size: 429 206 27 41 516 1392 3507 Fragments arranged by size: 3507 1392 516 429 206 41 27 BsrDI GCAATG_nn' Cuts at: 65 1248 2952 4613 4787 65 Size: 1183 1704 1661 174 1396 Fragments arranged by size: 1704 1661 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2070 2139 2410 4762 4949 1700 Size: 110 260 69 271 2352 187 2869 Fragments arranged by size: 2869 2352 271 260 187 110 69 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 2249 4178 5190 2249 Size: 1929 1012 3177 Fragments arranged by size: 3177 1929 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6118 BstBI TT'CG_AA Cuts at: 4922 4922 Size: 6118 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstXI CCAn_nnnn'nTGG Cuts at: 2549 3364 2549 Size: 815 5303 BstYI r'GATC_y Cuts at: 1972 2679 3240 3285 3448 3815 4200 4217 5669 Size: 707 561 45 163 367 385 17 1452 Cuts at: 5669 5755 5767 6113 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 707 561 385 367 346 163 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 3150 3150 Size: 6118 BtgI C'CryG_G Cuts at: 513 1065 2418 2889 513 Size: 552 1353 471 3742 Fragments arranged by size: 3742 1353 552 471 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 2358 3634 4428 4448 2358 Size: 1276 794 20 4028 Fragments arranged by size: 4028 1276 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2936 5125 809 Size: 507 1620 2189 1802 Fragments arranged by size: 2189 1802 1620 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3106 3565 4454 Size: 54 1425 50 275 18 1276 459 889 Cuts at: 4454 6083 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1276 889 459 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2370 4048 1353 Size: 1017 1678 3423 Fragments arranged by size: 3423 1678 1017 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3087 3087 Size: 6118 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4181 4778 5565 5833 1239 Size: 393 2549 597 787 268 1524 Fragments arranged by size: 2549 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2876 2876 Size: 6118 EcoO109I rG'GnC_Cy Cuts at: 2545 2853 3121 3334 2545 Size: 308 268 213 5329 Fragments arranged by size: 5329 308 268 213 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 2166 2211 2455 5265 1678 Size: 488 45 244 2810 2531 Fragments arranged by size: 2810 2531 488 244 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3354 3377 3386 3409 4759 Size: 32 135 32 1782 23 9 23 1350 Cuts at: 4759 4791 4833 4865 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1782 1350 135 42 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 3462 3708 669 Size: 988 281 1524 246 3079 Fragments arranged by size: 3079 1524 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6118 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KasI G'GCGC_C Cuts at: 2451 2451 Size: 6118 KpnI G_GTAC'C Cuts at: 3443 3443 Size: 6118 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 5232 5416 1305 Size: 684 3243 184 2007 Fragments arranged by size: 3243 2007 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4296 Fragments arranged by size: 4296 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 3137 3305 4076 4435 4594 518 Size: 648 1149 822 168 771 359 159 2042 Fragments arranged by size: 2042 1149 822 771 648 359 168 159 MspA1I CmG'CkG Cuts at: 1606 2158 2977 3534 4211 5359 5604 1606 Size: 552 819 557 677 1148 245 2120 Fragments arranged by size: 2120 1148 819 677 557 552 245 NarI GG'CG_CC Cuts at: 2452 2452 Size: 6118 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5566 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 2719 2974 3476 5021 1132 Size: 97 1490 255 502 1545 2229 Fragments arranged by size: 2229 1545 1490 502 255 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 2638 1108 Size: 1530 4588 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3121 3121 Size: 6118 PshAI GACnn'nnGTC Cuts at: 1613 3119 1613 Size: 1506 4612 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PspOMI G'GGCC_C Cuts at: 2852 2852 Size: 6118 PstI C_TGCA'G Cuts at: 830 3470 830 Size: 2640 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 PvuII CAG'CTG Cuts at: 2977 2977 Size: 6118 SacI G_AGCT'C Cuts at: 3089 3089 Size: 6118 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SapI GCTCTTCn'nnn_ Cuts at: 2370 2370 Size: 6118 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SexAI A'CCwGG_T Cuts at: 2823 2871 2823 Size: 48 6070 SfcI C'TryA_G Cuts at: 826 1045 3466 4601 5282 5473 826 Size: 219 2421 1135 681 191 1471 Fragments arranged by size: 2421 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3342 3342 Size: 6118 SfoI GGC'GCC Cuts at: 2453 2453 Size: 6118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 2410 1700 Size: 710 5408 SmaI CCC'GGG Cuts at: 1872 2171 3445 1872 Size: 299 1274 4545 Fragments arranged by size: 4545 1274 299 SmlI C'TyrA_G Cuts at: 820 1017 1943 2858 4222 5123 5385 820 Size: 197 926 915 1364 901 262 1553 Fragments arranged by size: 1553 1364 926 915 901 262 197 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 2974 3476 1132 Size: 1842 502 3774 Fragments arranged by size: 3774 1842 502 SspI AAT'ATT Cuts at: 5 52 4042 5 Size: 47 3990 2081 Fragments arranged by size: 3990 2081 47 StuI AGG'CCT Cuts at: 1517 2689 2864 1517 Size: 1172 175 4771 Fragments arranged by size: 4771 1172 175 StyI C'CwwG_G Cuts at: 513 1065 2901 3557 513 Size: 552 1836 656 3074 Fragments arranged by size: 3074 1836 656 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4145 4162 4315 4500 4945 325 Size: 1255 2565 17 153 185 445 1498 Fragments arranged by size: 2565 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 4364 6076 Size: 274 80 33 51 1443 345 2042 1712 Cuts at: 6076 96 Size: 138 Fragments arranged by size: 2042 1712 1443 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2664 2696 6078 6110 2664 Size: 32 3382 32 2672 Fragments arranged by size: 3382 2672 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2825 3260 1316 Size: 1509 435 4174 Fragments arranged by size: 4174 1509 435 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2708 2708 Size: 6118 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6118 XmaI C'CCGG_G Cuts at: 1870 2169 3443 1870 Size: 299 1274 4545 Fragments arranged by size: 4545 1274 299 XmnI GAAnn'nnTTC Cuts at: 4247 4247 Size: 6118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SrfI SwaI