(Circular) (Six-base) MAPSORT of: pFN21AB5808.seq Check: 2504 from: 1 to: 6067 pFN21AB5808 6067 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2262 2633 3410 1937 Size: 325 371 777 4594 Fragments arranged by size: 4594 777 371 325 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3224 3374 4130 5514 5782 1632 Size: 1592 150 756 1384 268 1917 Fragments arranged by size: 1917 1592 1384 756 268 150 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 2376 2378 3126 4966 5943 1203 Size: 1173 2 748 1840 977 1327 Fragments arranged by size: 1840 1327 1173 977 748 2 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 2959 3810 5382 2959 Size: 851 1572 3644 Fragments arranged by size: 3644 1572 851 ApaI G_GGCC'C Cuts at: 2922 2922 Size: 6067 ApaLI G'TGCA_C Cuts at: 4124 5280 4124 Size: 1156 4911 ApoI r'AATT_y Cuts at: 1493 3376 3602 3754 5863 1493 Size: 1883 226 152 2109 1697 Fragments arranged by size: 2109 1883 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 2256 3392 1870 Size: 73 313 1136 4545 Fragments arranged by size: 4545 1136 313 73 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2224 3102 3388 4843 Size: 325 217 627 252 185 878 286 1455 Cuts at: 4843 618 Size: 1842 Fragments arranged by size: 1842 1455 878 627 325 286 252 217 185 BanII G_rGCy'C Cuts at: 1391 2135 2555 2571 2766 2922 3045 3386 1391 Size: 744 420 16 195 156 123 341 4072 Fragments arranged by size: 4072 744 420 341 195 156 123 16 BbeI G_GCGC'C Cuts at: 3106 3106 Size: 6067 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5096 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4258 4292 1903 Size: 34 2321 34 3678 Fragments arranged by size: 3678 2321 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2625 4678 136 Size: 107 122 71 631 605 953 2053 1525 Fragments arranged by size: 2053 1525 953 631 605 122 107 71 BglII A'GATC_T Cuts at: 6062 6062 Size: 6067 BlpI GC'TnA_GC Cuts at: 2129 2549 2765 2996 3484 2129 Size: 420 216 231 488 4712 Fragments arranged by size: 4712 488 420 231 216 Bme1580I G_kGCm'C Cuts at: 1790 2044 2101 2229 2922 4128 5284 1790 Size: 254 57 128 693 1206 1156 2573 Fragments arranged by size: 2573 1206 1156 693 254 128 57 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3798 4756 442 Size: 416 53 83 399 24 2381 958 1753 Fragments arranged by size: 2381 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2197 3295 2197 Size: 1098 4969 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2629 2987 3056 3272 4727 1239 Size: 1390 358 69 216 1455 2579 Fragments arranged by size: 2579 1455 1390 358 216 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4192 5057 5355 4192 Size: 865 298 4904 Fragments arranged by size: 4904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2115 3015 4730 882 Size: 617 616 900 1715 2219 Fragments arranged by size: 2219 1715 900 617 616 BsaAI yAC'GTr Cuts at: 493 1204 2379 3858 493 Size: 711 1175 1479 2702 Fragments arranged by size: 2702 1479 1175 711 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3103 3874 4256 4891 Size: 53 83 186 936 1570 771 382 635 Cuts at: 4891 275 Size: 1451 Fragments arranged by size: 1570 1451 936 771 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2493 2889 4500 4898 5172 5319 5730 2493 Size: 396 1611 398 274 147 411 2830 Fragments arranged by size: 2830 1611 411 398 396 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5842 Fragments arranged by size: 5842 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2244 3053 3271 1186 Size: 1045 13 809 218 3982 Fragments arranged by size: 3982 1045 809 218 13 BseYI C'CCAG_C Cuts at: 2228 2623 2948 3113 3361 5270 5766 2228 Size: 395 325 165 248 1909 496 2529 Fragments arranged by size: 2529 1909 496 395 325 248 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2670 2684 2815 1163 Size: 573 934 14 131 4415 Fragments arranged by size: 4415 934 573 131 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2207 2571 4128 4213 5284 2207 Size: 364 1557 85 1071 2990 Fragments arranged by size: 2990 1557 1071 364 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2101 2135 2207 2229 2555 2571 Size: 399 254 57 34 72 22 326 16 Cuts at: 2571 2766 2922 3045 3386 4128 4213 5284 1391 Size: 195 156 123 341 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 341 326 254 195 156 123 85 72 57 34 22 16 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 3956 5686 3956 Size: 1730 4337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2063 3422 844 Size: 1219 1359 3489 Fragments arranged by size: 3489 1359 1219 BsrBI CCG'CTC Cuts at: 1394 1823 3954 1394 Size: 429 2131 3507 Fragments arranged by size: 3507 2131 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2290 2769 4562 4736 65 Size: 1183 1042 479 1793 174 1396 Fragments arranged by size: 1793 1396 1183 1042 479 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2439 2909 3105 4711 4898 1700 Size: 110 629 470 196 1606 187 2869 Fragments arranged by size: 2869 1606 629 470 196 187 110 BsrGI T'GTAC_A Cuts at: 96 2373 96 Size: 2277 3790 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 2959 794 Size: 2165 3902 BstBI TT'CG_AA Cuts at: 2457 4871 2457 Size: 2414 3653 BstEII G'GTnAC_C Cuts at: 1184 2413 2529 1184 Size: 1229 116 4722 Fragments arranged by size: 4722 1229 116 BstXI CCAn_nnnn'nTGG Cuts at: 2176 2176 Size: 6067 BstYI r'GATC_y Cuts at: 1972 2054 2671 3397 3764 4149 4166 5618 5704 Size: 82 617 726 367 385 17 1452 86 Cuts at: 5704 5716 6062 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 726 617 385 367 346 86 82 17 12 BstZ17I GTA'TAC Cuts at: 2634 2634 Size: 6067 Bsu36I CC'TnA_GG Cuts at: 3086 3086 Size: 6067 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4576 Fragments arranged by size: 4576 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 3071 3583 4377 4397 3071 Size: 512 794 20 4741 Fragments arranged by size: 4741 794 512 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DraIII CAC_nnn'GTG Cuts at: 2662 2662 Size: 6067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3514 4403 Size: 54 1425 50 275 18 177 1507 889 Cuts at: 4403 6032 8 Size: 1629 43 Fragments arranged by size: 1629 1507 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2559 3997 1353 Size: 1206 1438 3423 Fragments arranged by size: 3423 1438 1206 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2103 2238 4654 5028 5174 387 Size: 1064 138 514 135 2416 374 146 1280 Fragments arranged by size: 2416 1280 1064 514 374 146 138 135 EcoICRI GAG'CTC Cuts at: 2569 2569 Size: 6067 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2629 2987 3056 3224 3272 3374 4130 Size: 393 997 358 69 168 48 102 756 Cuts at: 4130 4727 5514 5782 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 997 787 756 597 393 358 268 168 102 69 48 EcoNI CCTnn'n_nnAGG Cuts at: 2168 2168 Size: 6067 EcoO109I rG'GnC_Cy Cuts at: 2048 2146 2919 3349 2048 Size: 98 773 430 4766 Fragments arranged by size: 4766 773 430 98 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 EcoRV GAT'ATC Cuts at: 2812 2812 Size: 6067 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2290 2359 2290 Size: 69 5998 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 3003 3106 5214 1678 Size: 1325 103 2108 2531 Fragments arranged by size: 2531 2108 1325 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2167 2199 2279 2311 2407 Size: 32 135 32 595 32 80 32 96 Cuts at: 2407 2439 3266 3298 4708 4740 4782 4814 1373 Size: 32 827 32 1410 32 42 32 2626 Fragments arranged by size: 2626 1410 827 595 135 96 80 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3411 3657 669 Size: 988 281 1473 246 3079 Fragments arranged by size: 3079 1473 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KasI G'GCGC_C Cuts at: 3102 3102 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 3666 3666 Size: 6067 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2895 5181 5365 1305 Size: 1590 2286 184 2007 Fragments arranged by size: 2286 2007 1590 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4068 Fragments arranged by size: 4068 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3303 4025 4384 4543 518 Size: 648 2137 722 359 159 2042 Fragments arranged by size: 2137 2042 722 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2623 2668 3483 4160 5308 5553 1606 Size: 1017 45 815 677 1148 245 2120 Fragments arranged by size: 2120 1148 1017 815 677 245 45 NaeI GCC'GGC Cuts at: 2441 2911 3107 2441 Size: 470 196 5401 Fragments arranged by size: 5401 470 196 NarI GG'CG_CC Cuts at: 3103 3103 Size: 6067 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4576 Fragments arranged by size: 4576 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NgoMIV G'CCGG_C Cuts at: 2439 2909 3105 2439 Size: 470 196 5401 Fragments arranged by size: 5401 470 196 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 2470 3130 3425 4970 1132 Size: 97 1241 660 295 1545 2229 Fragments arranged by size: 2229 1545 1241 660 295 97 PasI CC'CwG_GG Cuts at: 1348 2044 2066 2093 1348 Size: 696 22 27 5322 Fragments arranged by size: 5322 696 27 22 PciI A'CATG_T Cuts at: 3126 4966 3126 Size: 1840 4227 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6067 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PmlI CAC'GTG Cuts at: 2379 2379 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PspOMI G'GGCC_C Cuts at: 2918 2918 Size: 6067 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2357 2389 2357 Size: 32 6035 PstI C_TGCA'G Cuts at: 830 3066 3419 830 Size: 2236 353 3478 Fragments arranged by size: 3478 2236 353 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2623 2668 2623 Size: 45 6022 SacI G_AGCT'C Cuts at: 2571 2571 Size: 6067 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SapI GCTCTTCn'nnn_ Cuts at: 2559 2559 Size: 6067 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 2435 4315 6027 2435 Size: 1880 1712 2475 Fragments arranged by size: 2475 1880 1712 SfcI C'TryA_G Cuts at: 826 1045 2444 3062 3415 4550 5231 5422 826 Size: 219 1399 618 353 1135 681 191 1471 Fragments arranged by size: 1471 1399 1135 681 618 353 219 191 SfoI GGC'GCC Cuts at: 3104 3104 Size: 6067 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 3105 1700 Size: 1405 4662 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4171 5072 5334 820 Size: 197 926 2228 901 262 1553 Fragments arranged by size: 2228 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6067 StyI C'CwwG_G Cuts at: 513 1065 2004 2169 2472 2577 3134 3506 513 Size: 552 939 165 303 105 557 372 3074 Fragments arranged by size: 3074 939 557 552 372 303 165 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4094 4111 4264 4449 4894 325 Size: 1255 2514 17 153 185 445 1498 Fragments arranged by size: 2514 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2373 2433 4313 Size: 274 80 33 51 1443 396 60 1880 Cuts at: 4313 6025 96 Size: 1712 138 Fragments arranged by size: 1880 1712 1443 396 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6027 6059 6027 Size: 32 6035 Tth111I GACn'n_nGTC Cuts at: 1316 2933 1316 Size: 1617 4450 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6067 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 2453 4196 2453 Size: 1743 4324 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI