Restriction map for pFN21AB5808
 (Linear) (Six-base) MAPSORT of: KIBB5808.seq  Check: 8148  from: 1  to: 1362



KIBB5808.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AccI GT'mk_AC

Cuts at:      0     257     628    1362 
   Size:        257     371     734

  Fragments arranged by size: 

                 734     371     257
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1219    1362 
   Size:       1219     143
 
AflIII A'CryG_T

Cuts at:      0     371     373    1121    1362 
   Size:        371       2     748     241

  Fragments arranged by size: 

                 748     371     241       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0     954    1362 
   Size:        954     408
 
ApaI G_GGCC'C

Cuts at:      0     917    1362 
   Size:        917     445
 
AvaI C'yCGr_G

Cuts at:      0     251    1362 
   Size:        251    1111
 
BanI G'GyrC_C

Cuts at:      0      34     219    1097    1362 
   Size:         34     185     878     265

  Fragments arranged by size: 

                 878     265     185      34
 
BanII G_rGCy'C

Cuts at:      0     130     550     566     761     917    1040    1362 
   Size:        130     420      16     195     156     123     322

  Fragments arranged by size: 

                 420     322     195     156     130     123      16
 
BbeI G_GCGC'C

Cuts at:      0    1101    1362 
   Size:       1101     261
 
BglI GCCn_nnn'nGGC

Cuts at:      0     620    1362 
   Size:        620     742
 
BlpI GC'TnA_GC

Cuts at:      0     124     544     760     991    1362 
   Size:        124     420     216     231     371

  Fragments arranged by size: 

                 420     371     231     216     124
 
Bme1580I G_kGCm'C

Cuts at:      0      39      96     224     917    1362 
   Size:         39      57     128     693     445

  Fragments arranged by size: 

                 693     445     128      57      39
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     192    1290    1362 
   Size:        192    1098      72

  Fragments arranged by size: 

                1098     192      72
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     624     982    1051    1267    1362 
   Size:        624     358      69     216      95

  Fragments arranged by size: 

                 624     358     216      95      69
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     110    1010    1362 
   Size:        110     900     352

  Fragments arranged by size: 

                 900     352     110
 
BsaAI yAC'GTr

Cuts at:      0     374    1362 
   Size:        374     988
 
BsaHI Gr'CG_yC

Cuts at:      0    1098    1362 
   Size:       1098     264
 
BsaWI w'CCGG_w

Cuts at:      0     488     884    1362 
   Size:        488     396     478

  Fragments arranged by size: 

                 488     478     396
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     226     239    1048    1266    1362 
   Size:        226      13     809     218      96

  Fragments arranged by size: 

                 809     226     218      96      13
 
BseYI C'CCAG_C

Cuts at:      0     223     618     943    1108    1356    1362 
   Size:        223     395     325     165     248       6

  Fragments arranged by size: 

                 395     325     248     223     165       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665     679     810    1362 
   Size:        665      14     131     552

  Fragments arranged by size: 

                 665     552     131      14
 
BsiHKAI G_wGCw'C

Cuts at:      0     202     566    1362 
   Size:        202     364     796

  Fragments arranged by size: 

                 796     364     202
 
Bsp1286I G_dGCh'C

Cuts at:      0      39      96     130     202     224     550     566     761 
   Size:         39      57      34      72      22     326      16     195

Cuts at:    761     917    1040    1362 
   Size:        156     123     322

  Fragments arranged by size: 

                 326     322     195     156     123      72      57      39
                  34      22      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      58    1362 
   Size:         58    1304
 
BsrDI GCAATG_nn'

Cuts at:      0     285     764    1362 
   Size:        285     479     598

  Fragments arranged by size: 

                 598     479     285
 
BsrFI r'CCGG_y

Cuts at:      0     434     904    1100    1362 
   Size:        434     470     196     262

  Fragments arranged by size: 

                 470     434     262     196
 
BsrGI T'GTAC_A

Cuts at:      0     368    1362 
   Size:        368     994
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     954    1362 
   Size:        954     408
 
BstBI TT'CG_AA

Cuts at:      0     452    1362 
   Size:        452     910
 
BstEII G'GTnAC_C

Cuts at:      0     408     524    1362 
   Size:        408     116     838

  Fragments arranged by size: 

                 838     408     116
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     171    1362 
   Size:        171    1191
 
BstYI r'GATC_y

Cuts at:      0      49     666    1362 
   Size:         49     617     696

  Fragments arranged by size: 

                 696     617      49
 
BstZ17I GTA'TAC

Cuts at:      0     629    1362 
   Size:        629     733
 
Bsu36I CC'TnA_GG

Cuts at:      0    1081    1362 
   Size:       1081     281
 
BtsI GCAGTG_nn'

Cuts at:      0    1066    1362 
   Size:       1066     296
 
DraIII CAC_nnn'GTG

Cuts at:      0     657    1362 
   Size:        657     705
 
EaeI y'GGCC_r

Cuts at:      0       2    1362 
   Size:          2    1360
 
EarI CTCTTCn'nnn_

Cuts at:      0     554    1362 
   Size:        554     808
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      98     233    1362 
   Size:         98     135    1129

  Fragments arranged by size: 

                1129     135      98
 
EcoICRI GAG'CTC

Cuts at:      0     564    1362 
   Size:        564     798
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     624     982    1051    1219    1267    1362 
   Size:        624     358      69     168      48      95

  Fragments arranged by size: 

                 624     358     168      95      69      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     163    1362 
   Size:        163    1199
 
EcoO109I rG'GnC_Cy

Cuts at:      0      43     141     914    1344    1362 
   Size:         43      98     773     430      18

  Fragments arranged by size: 

                 773     430      98      43      18
 
EcoRV GAT'ATC

Cuts at:      0     807    1362 
   Size:        807     555
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     285     354    1362 
   Size:        285      69    1008

  Fragments arranged by size: 

                1008     285      69
 
HaeII r_GCGC'y

Cuts at:      0     998    1101    1362 
   Size:        998     103     261

  Fragments arranged by size: 

                 998     261     103
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     162     194     274     306     402     434    1261    1293 
   Size:        162      32      80      32      96      32     827      32

Cuts at:   1293    1362 
   Size:         69

  Fragments arranged by size: 

                 827     162      96      80      69      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0    1097    1362 
   Size:       1097     265
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     890    1362 
   Size:        890     472
 
MscI TGG'CCA

Cuts at:      0       4    1362 
   Size:          4    1358
 
MslI CAynn'nnrTG

Cuts at:      0    1298    1362 
   Size:       1298      64
 
MspA1I CmG'CkG

Cuts at:      0     618     663    1362 
   Size:        618      45     699

  Fragments arranged by size: 

                 699     618      45
 
NaeI GCC'GGC

Cuts at:      0     436     906    1102    1362 
   Size:        436     470     196     260

  Fragments arranged by size: 

                 470     436     260     196
 
NarI GG'CG_CC

Cuts at:      0    1098    1362 
   Size:       1098     264
 
NgoMIV G'CCGG_C

Cuts at:      0     434     904    1100    1362 
   Size:        434     470     196     262

  Fragments arranged by size: 

                 470     434     262     196
 
NspI r_CATG'y

Cuts at:      0     465    1125    1362 
   Size:        465     660     237

  Fragments arranged by size: 

                 660     465     237
 
PasI CC'CwG_GG

Cuts at:      0      39      61      88    1362 
   Size:         39      22      27    1274

  Fragments arranged by size: 

                1274      39      27      22
 
PciI A'CATG_T

Cuts at:      0    1121    1362 
   Size:       1121     241
 
PmlI CAC'GTG

Cuts at:      0     374    1362 
   Size:        374     988
 
PspOMI G'GGCC_C

Cuts at:      0     913    1362 
   Size:        913     449
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     352     384    1362 
   Size:        352      32     978

  Fragments arranged by size: 

                 978     352      32
 
PstI C_TGCA'G

Cuts at:      0    1061    1362 
   Size:       1061     301
 
PvuII CAG'CTG

Cuts at:      0     618     663    1362 
   Size:        618      45     699

  Fragments arranged by size: 

                 699     618      45
 
SacI G_AGCT'C

Cuts at:      0     566    1362 
   Size:        566     796
 
SapI GCTCTTCn'nnn_

Cuts at:      0     554    1362 
   Size:        554     808
 
ScaI AGT'ACT

Cuts at:      0     430    1362 
   Size:        430     932
 
SfcI C'TryA_G

Cuts at:      0     439    1057    1362 
   Size:        439     618     305

  Fragments arranged by size: 

                 618     439     305
 
SfoI GGC'GCC

Cuts at:      0    1099    1362 
   Size:       1099     263
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1100    1362 
   Size:       1100     262
 
StyI C'CwwG_G

Cuts at:      0     164     467     572    1129    1362 
   Size:        164     303     105     557     233

  Fragments arranged by size: 

                 557     303     233     164     105
 
TatI w'GTAC_w

Cuts at:      0     368     428    1362 
   Size:        368      60     934

  Fragments arranged by size: 

                 934     368      60
 
Tth111I GACn'n_nGTC

Cuts at:      0     928    1362 
   Size:        928     434
 
XmnI GAAnn'nnTTC

Cuts at:      0     448    1362 
   Size:        448     914
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApaI     AvaI     BanI    BanII
     BbeI     BglI     BlpI Bme1580I     BplI     BpmI     BsaI    BsaAI
    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrDI    BsrFI    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtsI   DraIII     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PasI     PciI     PmlI   PspOMI     PsrI     PstI    PvuII     SacI
     SapI     ScaI     SfcI     SfoI    SgrAI     StyI     TatI  Tth111I
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII    BmgBI     BmrI     BmtI   Bpu10I    BpuEI    BsaBI
    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
   BssHII    BssSI     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI
     EagI    EcoRI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI     XbaI     XcmI
     XhoI     XmaI     ZraI
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