Restriction map for
pFN21AB7966
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BalI BamHI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglII BlpI BpmI Bpu1102I BpuAI
BsaAI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eam1105I EarI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
HphI KpnI Ksp632I MaeII MfeI
MluI MluNI MroI MscI MseI
MslI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tru9I Tth111I TthHB8I
Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 618 GTCTAC GT^[AC][GT]AC
AlwNI 413 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 307 GGGCCC GGGCC^C
AvrII 840 CCTAGG C^CTAGG
BanI 377 GGCGCC G^G[CT][GA]CC
BbeI 377 GGCGCC GGCGC^C
BcgI 528 GCAGATTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 309 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 840 CCTAGG C^CTAGG
BsaI 799 GAGACC GGTCTC(1/5)
BsaWI 1107 ACCGGA [AT]^CCGG[AT]
BsgI 526 GTGCAG GTGCAG(16/14)
Bsp1407I 817 TGTACA T^GTACA
BsrGI 817 TGTACA T^GTACA
BssHII 256 GCGCGC G^CGCGC
BstXI 184 CCAGCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 696 AGATCC [GA]^GATC[CT]
DraIII 1070 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 316 CGGCCG C^GGCCG
EclXI 316 CGGCCG C^GGCCG
Eco52I 316 CGGCCG C^GGCCG
HgaI 142 GCGTC GACGC(5/10)
KasI 377 GGCGCC G^GCGCC
MflI 696 AGATCC [GA]^GATC[CT]
NaeI 314 GCCGGC GCC^GGC
NarI 377 GGCGCC GG^CGCC
NcoI 85 CCATGG C^CATGG
NgoMIV 314 GCCGGC G^CCGGC
NspI 674 ACATGC [GA]CATG^[CT]
PpuMI 226 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1169 CTGCAG CTGCA^G
PvuII 821 CAGCTG CAG^CTG
SacI 747 GAGCTC GAGCT^C
SfiI 308 GGCCCCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 377 GGCGCC GGC^GCC
SgrAI 251 CACCGGCG C[GA]^CCGG[CT]G
SspBI 817 TGTACA T^GTACA
Tsp45I 919 GTGAC ^GT[GC]AC
Tsp509I 714 AATT ^AATT
XbaI 792 TCTAGA T^CTAGA
XhoII 696 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 141 GGCGTC G[GA]^CG[CT]C
377 GGCGCC
AlwI 697 GATCC GGATC(4/5)
877 GGATC
AvaI 103 CCCGGG C^[CT]CG[GA]G
873 CCCGGG
BsaBI 697 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
765 GATGGGCATC
BsaHI 141 GGCGTC G[GA]^CG[CT]C
377 GGCGCC
BsiEI 316 CGGCCG CG[GA][CT]^CG
959 CGGTCG
BsoBI 103 CCCGGG C^[CT]CG[GA]G
873 CCCGGG
BsrFI 252 ACCGGC [GA]^CCGG[CT]
314 GCCGGC
Cfr10I 252 ACCGGC [GA]^CCGG[CT]
314 GCCGGC
DraII 226 GGGTCCT [GA]G^G[ACGT]CC[CT]
307 GGGCCCC
EaeI 316 CGGCCG [CT]^GGCC[GA]
998 CGGCCA
EcoNI 510 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1113 CCTCAACCAGG
EcoO109I 226 GGGTCCT [GA]G^G[ACGT]CC[CT]
307 GGGCCCC
HaeII 186 AGCGCC [GA]GCGC^[CT]
377 GGCGCC
Hin1I 141 GGCGTC G[GA]^CG[CT]C
377 GGCGCC
KspI 159 CCGCGG CCGC^GG
337 CCGCGG
MamI 697 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
765 GATGGGCATC
MboII 469 TCTTC GAAGA(8/7)
534 TCTTC
SacII 159 CCGCGG CCGC^GG
337 CCGCGG
SfcI 625 CTACAG C^T[GA][CT]AG
1169 CTGCAG
SmaI 103 CCCGGG CCC^GGG
873 CCCGGG
XcmI 128 CCACGGCTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1119 CCAGGACTTCAGTGG
XmaCI 103 CCCGGG C^CCGGG
873 CCCGGG
XmaI 103 CCCGGG C^CCGGG
873 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeI MaeIII MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI TseI TspRI