Restriction map for pFN21AB7966
 (Linear) (Six-base) MAPSORT of: KIBB7966.seq  Check: 4212  from: 1  to: 1203



KIBB7966.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AccI GT'mk_AC

Cuts at:      0     619    1203 
   Size:        619     584
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     560    1110    1168    1203 
   Size:        560     550      58      35

  Fragments arranged by size: 

                 560     550      58      35
 
AlwNI CAG_nnn'CTG

Cuts at:      0     418    1203 
   Size:        418     785
 
ApaI G_GGCC'C

Cuts at:      0     311    1203 
   Size:        311     892
 
AvaI C'yCGr_G

Cuts at:      0     103     873    1203 
   Size:        103     770     330

  Fragments arranged by size: 

                 770     330     103
 
AvrII C'CTAG_G

Cuts at:      0     840    1203 
   Size:        840     363
 
BanI G'GyrC_C

Cuts at:      0     377    1203 
   Size:        377     826
 
BanII G_rGCy'C

Cuts at:      0      40      58     311     751    1203 
   Size:         40      18     253     440     452

  Fragments arranged by size: 

                 452     440     253      40      18
 
BbeI G_GCGC'C

Cuts at:      0     381    1203 
   Size:        381     822
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     517     551    1203 
   Size:        517      34     652

  Fragments arranged by size: 

                 652     517      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     315    1203 
   Size:        315     888
 
Bme1580I G_kGCm'C

Cuts at:      0     279     311    1203 
   Size:        279      32     892

  Fragments arranged by size: 

                 892     279      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     108    1203 
   Size:        108    1095
 
Bpu10I CC'TnA_GC

Cuts at:      0     483     860    1203 
   Size:        483     377     343

  Fragments arranged by size: 

                 483     377     343
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     793    1203 
   Size:        793     410
 
BsaBI GATnn'nnATC

Cuts at:      0     701     769    1203 
   Size:        701      68     434

  Fragments arranged by size: 

                 701     434      68
 
BsaHI Gr'CG_yC

Cuts at:      0     142     378    1203 
   Size:        142     236     825

  Fragments arranged by size: 

                 825     236     142
 
BsaWI w'CCGG_w

Cuts at:      0    1107    1203 
   Size:       1107      96
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     236     362     655     811    1082    1203 
   Size:        236     126     293     156     271     121

  Fragments arranged by size: 

                 293     271     236     156     126     121
 
BseYI C'CCAG_C

Cuts at:      0     183     291     304     602     823    1203 
   Size:        183     108      13     298     221     380

  Fragments arranged by size: 

                 380     298     221     183     108      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547    1203 
   Size:        547     656
 
BsiEI CG_ry'CG

Cuts at:      0     319     962    1203 
   Size:        319     643     241

  Fragments arranged by size: 

                 643     319     241
 
BsiHKAI G_wGCw'C

Cuts at:      0     121     557     751    1203 
   Size:        121     436     194     452

  Fragments arranged by size: 

                 452     436     194     121
 
Bsp1286I G_dGCh'C

Cuts at:      0      40      58     121     279     311     557     751    1203 
   Size:         40      18      63     158      32     246     194     452

  Fragments arranged by size: 

                 452     246     194     158      63      40      32      18
            
 
BsrFI r'CCGG_y

Cuts at:      0     252     314    1203 
   Size:        252      62     889

  Fragments arranged by size: 

                 889     252      62
 
BsrGI T'GTAC_A

Cuts at:      0     817    1203 
   Size:        817     386
 
BssHII G'CGCG_C

Cuts at:      0     256    1203 
   Size:        256     947
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     191    1203 
   Size:        191    1012
 
BstYI r'GATC_y

Cuts at:      0     696    1203 
   Size:        696     507
 
BtgI C'CryG_G

Cuts at:      0      85     128     159     190     337     723    1203 
   Size:         85      43      31      31     147     386     480

  Fragments arranged by size: 

                 480     386     147      85      43      31      31
 
BtsI GCAGTG_nn'

Cuts at:      0     429    1203 
   Size:        429     774
 
DraIII CAC_nnn'GTG

Cuts at:      0    1075    1203 
   Size:       1075     128
 
EaeI y'GGCC_r

Cuts at:      0     316     998    1203 
   Size:        316     682     205

  Fragments arranged by size: 

                 682     316     205
 
EagI C'GGCC_G

Cuts at:      0     316    1203 
   Size:        316     887
 
EcoICRI GAG'CTC

Cuts at:      0     749    1203 
   Size:        749     454
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     560    1110    1168    1203 
   Size:        560     550      58      35

  Fragments arranged by size: 

                 560     550      58      35
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     514    1117    1203 
   Size:        514     603      86

  Fragments arranged by size: 

                 603     514      86
 
EcoO109I rG'GnC_Cy

Cuts at:      0     227     308    1203 
   Size:        227      81     895

  Fragments arranged by size: 

                 895     227      81
 
HaeII r_GCGC'y

Cuts at:      0     190     381    1203 
   Size:        190     191     822

  Fragments arranged by size: 

                 822     191     190
 
KasI G'GCGC_C

Cuts at:      0     377    1203 
   Size:        377     826
 
MspA1I CmG'CkG

Cuts at:      0     161     339     478     823    1203 
   Size:        161     178     139     345     380

  Fragments arranged by size: 

                 380     345     178     161     139
 
NaeI GCC'GGC

Cuts at:      0     316    1203 
   Size:        316     887
 
NarI GG'CG_CC

Cuts at:      0     378    1203 
   Size:        378     825
 
NcoI C'CATG_G

Cuts at:      0      85    1203 
   Size:         85    1118
 
NgoMIV G'CCGG_C

Cuts at:      0     314    1203 
   Size:        314     889
 
NspI r_CATG'y

Cuts at:      0     678    1203 
   Size:        678     525
 
PasI CC'CwG_GG

Cuts at:      0     351    1203 
   Size:        351     852
 
PfoI T'CCnGG_A

Cuts at:      0     386    1203 
   Size:        386     817
 
PpuMI rG'GwC_Cy

Cuts at:      0     227    1203 
   Size:        227     976
 
PspOMI G'GGCC_C

Cuts at:      0     307    1203 
   Size:        307     896
 
PstI C_TGCA'G

Cuts at:      0    1173    1203 
   Size:       1173      30
 
PvuII CAG'CTG

Cuts at:      0     823    1203 
   Size:        823     380
 
SacI G_AGCT'C

Cuts at:      0     751    1203 
   Size:        751     452
 
SacII CC_GC'GG

Cuts at:      0     162     340    1203 
   Size:        162     178     863

  Fragments arranged by size: 

                 863     178     162
 
SfcI C'TryA_G

Cuts at:      0     625    1169    1203 
   Size:        625     544      34

  Fragments arranged by size: 

                 625     544      34
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     315    1203 
   Size:        315     888
 
SfoI GGC'GCC

Cuts at:      0     379    1203 
   Size:        379     824
 
SgrAI Cr'CCGG_yG

Cuts at:      0     252    1203 
   Size:        252     951
 
SmaI CCC'GGG

Cuts at:      0     105     875    1203 
   Size:        105     770     328

  Fragments arranged by size: 

                 770     328     105
 
StyI C'CwwG_G

Cuts at:      0      85     299     458     515     840    1203 
   Size:         85     214     159      57     325     363

  Fragments arranged by size: 

                 363     325     214     159      85      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     948    1203 
   Size:        948     255
 
TatI w'GTAC_w

Cuts at:      0     817    1203 
   Size:        817     386
 
XbaI T'CTAG_A

Cuts at:      0     792    1203 
   Size:        792     411
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     135    1126    1203 
   Size:        135     991      77

  Fragments arranged by size: 

                 991     135      77
 
XmaI C'CCGG_G

Cuts at:      0     103     873    1203 
   Size:        103     770     330

  Fragments arranged by size: 

                 770     330     103
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApaI     AvaI    AvrII     BanI    BanII
     BbeI     BcgI     BglI Bme1580I     BmrI   Bpu10I     BsaI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BsrFI    BsrGI   BssHII    BstXI    BstYI     BtgI     BtsI   DraIII
     EaeI     EagI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII     KasI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PfoI
    PpuMI   PspOMI     PstI    PvuII     SacI    SacII     SfcI     SfiI
     SfoI    SgrAI     SmaI     StyI    TaqII     TatI     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     BaeI    BamHI     BbsI    BbvCI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI    BpuEI    BsaAI
    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI     DrdI     EarI     EciI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SgfI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XhoI     XmnI     ZraI
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