Restriction map for
pFN21AB7934
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AvaII AviII AvrII BalI BanI
BanII BbeI BbrPI BbvCI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaI HpaII KasI KpnI KspI
MaeI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tru9I Tsp45I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 196 GCGG CCGC(-3/-1)
AcsI 175 AAATTT [GA]^AATT[CT]
ApoI 175 AAATTT [GA]^AATT[CT]
AspHI 132 GTGCTC G[AT]GC[AT]^C
AspI 150 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 270 GGATCC G^GATCC
BbsI 147 GAAGAC GAAGAC(2/6)
BcgI 60 GCAAACTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 132 GTGCTC G[AGT]GC[ACT]^C
BpuAI 147 GAAGAC GAAGAC(2/6)
BsiHKAI 132 GTGCTC G[AT]GC[AT]^C
BsiYI 238 CCTCCCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 238 CCTCCCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 250 GAGAC GTCTC(1/5)
Bsp1286I 132 GTGCTC G[AGT]GC[ACT]^C
BsrI 384 ACTGG ACTGG(1/-1)
BssKI 352 CCTGG ^CC[ACGT]GG
BstNI 352 CCTGG CC^[AT]GG
Cfr13I 204 GGCCC G^G[ACGT]CC
EcoNI 238 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 352 CCTGG ^CC[AT]GG
EcoT22I 47 ATGCAT ATGCA^T
HincII 106 GTCAAC GT[CT]^[GA]AC
HindII 106 GTCAAC GT[CT]^[GA]AC
HphI 367 GGTGA GGTGA(8/7)
MslI 91 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 352 CCTGG CC^[AT]GG
NdeI 44 CATATG CA^TATG
NlaIII 153 CATG CATG^
NlaIV 270 GGATCC GG[ACGT]^[ACGT]CC
NsiI 47 ATGCAT ATGCA^T
PflFI 150 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 410 CTGCAG CTGCA^G
Sau96I 204 GGCCC G^G[ACGT]CC
ScrFI 352 CCTGG CC^[ACGT]GG
SfcI 410 CTGCAG C^T[GA][CT]AG
StuI 289 AGGCCT AGG^CCT
TaqI 486 TCGA T^CGA
Tsp509I 176 AATT ^AATT
TspRI 405 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 150 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 486 TCGA T^CGA
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# TWO CUT ENZYMES ( 12 enzymes) #
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AluI 449 AGCT AG^CT
489 AGCT
BstF5I 349 CATCC GGATG(2/0)
585 GGATG
BstYI 12 AGATCC [GA]^GATC[CT]
270 GGATCC
DpnII 13 GATC ^GATC
271 GATC
FokI 349 CATCC GGATG(9/13)
585 GGATG
MboI 13 GATC ^GATC
271 GATC
MflI 12 AGATCC [GA]^GATC[CT]
270 GGATCC
MwoI 196 GCGGACAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
481 GCATATCGAGC
NdeII 13 GATC ^GATC
271 GATC
Sau3AI 13 GATC ^GATC
271 GATC
SfaNI 97 GATGC GCATC(5/9)
505 GATGC
XhoII 12 AGATCC [GA]^GATC[CT]
270 GGATCC
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AlwI BbvI BseRI DdeI EarI
Fnu4HI HaeIII ItaI Ksp632I MboII
MnlI TseI