Restriction map for
pFN21AB7928
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MaeIII MamI MfeI
MluI MseI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp45I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 325 TCCGGA T^CCGGA
BalI 341 TGGCCA TGG^CCA
BbsI 489 GAAGAC GAAGAC(2/6)
BclI 251 TGATCA T^GATCA
BpuAI 489 GAAGAC GAAGAC(2/6)
BsaWI 325 TCCGGA [AT]^CCGG[AT]
BseAI 325 TCCGGA T^CCGGA
BseRI 409 GAGGAG GAGGAG(10/8)
BsmFI 236 GGGAC GGGAC(10/14)
BspEI 325 TCCGGA T^CCGGA
BsrBI 24 CCGCTC CCGCTC(-3/-3)
BsrFI 225 GCCGGT [GA]^CCGG[CT]
BstAPI 50 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 300 CATCC GGATG(2/0)
Cfr10I 225 GCCGGT [GA]^CCGG[CT]
DdeI 535 CTGAG C^T[ACGT]AG
DsaI 368 CCATGG C^C[GA][CT]GG
EaeI 341 TGGCCA [CT]^GGCC[GA]
EcoT14I 368 CCATGG C^C[AT][AT]GG
FbaI 251 TGATCA T^GATCA
FokI 300 CATCC GGATG(9/13)
MluNI 341 TGGCCA TGG^CCA
MroI 325 TCCGGA T^CCGGA
MscI 341 TGGCCA TGG^CCA
MslI 73 CATCGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 368 CCATGG C^CATGG
PpuMI 236 GGGACCC [GA]G^G[AT]CC[CT]
PstI 151 CTGCAG CTGCA^G
SfaNI 263 GCATC GCATC(5/9)
SmaI 117 CCCGGG CCC^GGG
StyI 368 CCATGG C^C[AT][AT]GG
XmaCI 117 CCCGGG C^CCGGG
XmaI 117 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaI 117 CCCGGG C^[CT]CG[GA]G
574 CCCGAG
AvaII 63 GGACC G^G[AT]CC
237 GGACC
BanII 82 GGGCTC G[GA]GC[CT]^C
537 GAGCCC
BcnI 117 CCCGG CC^[GC]GG
118 CCGGG
BfaI 220 CTAG C^TAG
466 CTAG
BpmI 85 CTCCAG CTGGAG(16/14)
514 CTGGAG
BsiYI 60 CCTGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
438 CCGAGAGCTGG
BslI 60 CCTGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
438 CCGAGAGCTGG
BsoBI 117 CCCGGG C^[CT]CG[GA]G
574 CCCGAG
BsrI 6 CCAGT ACTGG(1/-1)
525 CCAGT
DraII 236 GGGACCC [GA]G^G[ACGT]CC[CT]
578 AGGCCCC
EarI 307 CTCTTC CTCTTC(1/4)
544 GAAGAG
EcoO109I 236 GGGACCC [GA]G^G[ACGT]CC[CT]
578 AGGCCCC
FauI 101 GCGGG CCCGC(4/6)
234 GCGGG
HphI 378 GGTGA GGTGA(8/7)
28 TCACC
Ksp632I 307 CTCTTC CTCTTC(1/4)
544 GAAGAG
MaeI 220 CTAG C^TAG
466 CTAG
NciI 117 CCCGG CC^[GC]GG
118 CCGGG
NlaIII 291 CATG CATG^
369 CATG
PvuII 148 CAGCTG CAG^CTG
511 CAGCTG
SapI 544 GAAGAGC GCTCTTC(1/4)
306 GCTCTTC
SfcI 128 CTACAG C^T[GA][CT]AG
151 CTGCAG
TspRI 131 CAGTG CA[GC]TG[ACGT][ACGT]^
318 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwI BbvI BmyI
BsaJI Bsp1286I BssKI BstNI BstXI
BstYI Cac8I Cfr13I DpnII EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI TaqI
TfiI TseI TthHB8I XhoII