Restriction map for
pFN21AB7909
##################################
# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglII BlnI BpmI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AfaI 204 GTAC GT^AC
AocI 109 CCTCAGG CC^T[ACGT]AGG
AvaII 378 GGTCC G^G[AT]CC
BanII 283 GAGCCC G[GA]GC[CT]^C
BbsI 228 GAAGAC GAAGAC(2/6)
BciVI 390 GGATAC GTATCC(6/5)
BcnI 47 CCCGG CC^[GC]GG
BfaI 43 CTAG C^TAG
BlpI 273 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 273 GCTGAGC GC^T[ACGT]AGC
BpuAI 228 GAAGAC GAAGAC(2/6)
BsgI 178 CTGCAC GTGCAG(16/14)
BsiEI 73 CGGCCG CG[GA][CT]^CG
BsmFI 379 GTCCC GGGAC(10/14)
BspDI 211 ATCGAT AT^CGAT
BspMI 161 ACCTGC ACCTGC(4/8)
BssHII 23 GCGCGC G^CGCGC
BstF5I 84 GGATG GGATG(2/0)
BstXI 341 CCAGGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 109 CCTCAGG CC^T[ACGT]AGG
CelII 273 GCTGAGC GC^T[ACGT]AGC
Cfr13I 378 GGTCC G^G[ACGT]CC
ClaI 211 ATCGAT AT^CGAT
DraII 377 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 359 CCACGG C^C[GA][CT]GG
EaeI 73 CGGCCG [CT]^GGCC[GA]
EagI 73 CGGCCG C^GGCCG
EarI 298 GAAGAG CTCTTC(1/4)
EclXI 73 CGGCCG C^GGCCG
Eco52I 73 CGGCCG C^GGCCG
Eco81I 109 CCTCAGG CC^T[ACGT]AGG
EcoNI 194 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 377 AGGTCCC [GA]G^G[ACGT]CC[CT]
FokI 84 GGATG GGATG(9/13)
HgaI 278 GCGTC GACGC(5/10)
HphI 93 TCACC GGTGA(8/7)
Ksp632I 298 GAAGAG CTCTTC(1/4)
MaeI 43 CTAG C^TAG
MslI 360 CACGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 47 CCCGG CC^[GC]GG
PpuMI 377 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 271 CAGCTG CAG^CTG
RsaI 204 GTAC GT^AC
Sau96I 378 GGTCC G^G[ACGT]CC
SfcI 288 CTACAG C^T[GA][CT]AG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 101 AGCT AG^CT
272 AGCT
BamHI 115 GGATCC G^GATCC
325 GGATCC
BglI 14 GCCGGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
336 GCCCCCCAGGC
BstNI 166 CCAGG CC^[AT]GG
341 CCAGG
BstYI 115 GGATCC [GA]^GATC[CT]
325 GGATCC
EcoRII 166 CCAGG ^CC[AT]GG
341 CCAGG
FauI 11 CCCGC CCCGC(4/6)
316 GCGGG
HinfI 90 GATTC G^A[ACGT]TC
214 GATTC
MboII 228 GAAGA GAAGA(8/7)
298 GAAGA
MflI 115 GGATCC [GA]^GATC[CT]
325 GGATCC
MvaI 166 CCAGG CC^[AT]GG
341 CCAGG
TfiI 90 GATTC G^A[AT]TC
214 GATTC
TspRI 353 CAGTG CA[GC]TG[ACGT][ACGT]^
372 CACTG
XhoII 115 GGATCC [GA]^GATC[CT]
325 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccII AciI AlwI BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstUI Cac8I CfoI DdeI DpnII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MnlI MspA1I
MspI MvnI MwoI NdeII NlaIV
Sau3AI ScrFI TaqI TseI TthHB8I