Restriction map for pFN21AB7909
 (Circular) (Six-base) MAPSORT of: pFN21AB7909.seq  Check: 7594  from: 1  to: 5107



pFN21AB7909  5107 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:29  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2917     278 
   Size:         53      83     186     936    1381    2468

  Fragments arranged by size: 

                2468    1381     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2450    1937 
   Size:        513    4594
 
Acc65I G'GTAC_C

Cuts at:   2428    2428 
   Size:       5107
 
AclI AA'CG_TT

Cuts at:   1563    3234    3607    1563 
   Size:       1671     373    3063

  Fragments arranged by size: 

                3063    1671     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2252    2291    3170    4554    4822    1632 
   Size:        620      39     879    1384     268    1917

  Fragments arranged by size: 

                1917    1384     879     620     268      39
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4910
 
AflIII A'CryG_T

Cuts at:   1203    4006    4983    1203 
   Size:       2803     977    1327

  Fragments arranged by size: 

                2803    1327     977
 
AgeI A'CCGG_T

Cuts at:   3938    3938 
   Size:       5107
 
AhdI GACnn_n'nnGTC

Cuts at:   3836    3836 
   Size:       5107
 
AleI CACnn'nnGTG

Cuts at:   1166    2369    1166 
   Size:       1203    3904
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5107
 
AlwNI CAG_nnn'CTG

Cuts at:   2850    4422    2850 
   Size:       1572    3535
 
ApaLI G'TGCA_C

Cuts at:   3164    4320    3164 
   Size:       1156    3951
 
ApoI r'AATT_y

Cuts at:   1493    2416    2642    2794    4903    1493 
   Size:        923     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     923     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3661     160 
   Size:        872    2629    1606

  Fragments arranged by size: 

                2629    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5107
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2432    1870 
   Size:         73     489    4545

  Fragments arranged by size: 

                4545     489      73
 
BamHI G'GATC_C

Cuts at:   2120    2330    2437    2804    2120 
   Size:        210     107     367    4423

  Fragments arranged by size: 

                4423     367     210     107
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2428    3883     618 
   Size:        325     217     627     641    1455    1842

  Fragments arranged by size: 

                1842    1455     641     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2292    2426    1391 
   Size:        901     134    4072

  Fragments arranged by size: 

                4072     901     134
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2240     928 
   Size:        971     341    3795

  Fragments arranged by size: 

                3795     971     341
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3298    3332    1903 
   Size:         34    1361      34    3678

  Fragments arranged by size: 

                3678    1361      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2389    2999    4209    2389 
   Size:        610    1210    3287

  Fragments arranged by size: 

                3287    1210     610
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5107
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2025    2347    3718 
   Size:        107     122      71     631     605     353     322    1371

Cuts at:   3718     136 
   Size:       1525

  Fragments arranged by size: 

                1525    1371     631     605     353     322     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5102    5102 
   Size:       5107
 
BlpI GC'TnA_GC

Cuts at:   2279    2524    2279 
   Size:        245    4862
 
Bme1580I G_kGCm'C

Cuts at:   1790    2343    2364    3168    4324    1790 
   Size:        553      21     804    1156    2573

  Fragments arranged by size: 

                2573    1156     804     553      21
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5107
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2838    3796     442 
   Size:        416      53      83     399      24    1421     958    1753

  Fragments arranged by size: 

                1753    1421     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5107
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3767    1239 
   Size:       2528    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3232    4097    4395    3232 
   Size:        865     298    3944

  Fragments arranged by size: 

                3944     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3770     882 
   Size:        617    2271    2219

  Fragments arranged by size: 

                2271    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2898     493 
   Size:        711    1694    2702

  Fragments arranged by size: 

                2702    1694     711
 
BsaBI GATnn'nnATC

Cuts at:   5101    5101 
   Size:       5107
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2914    3296    3931     275 
   Size:         53      83     186     936    1381     382     635    1451

  Fragments arranged by size: 

                1451    1381     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3540    3938    4212    4359    4770    3540 
   Size:        398     274     147     411    3877

  Fragments arranged by size: 

                3877     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4882

  Fragments arranged by size: 

                4882     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5107
 
BseYI C'CCAG_C

Cuts at:   2133    4310    4806    2133 
   Size:       2177     496    2434

  Fragments arranged by size: 

                2434    2177     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2168    1163 
   Size:        573     432    4102

  Fragments arranged by size: 

                4102     573     432
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2081    2557    3318    4346    1540 
   Size:        275     186      80     476     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     476     275     186      80
 
BsiHKAI G_wGCw'C

Cuts at:   3168    3253    4324    3168 
   Size:         85    1071    3951

  Fragments arranged by size: 

                3951    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2618    2711    2618 
   Size:         93    5014
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2292    2343    2364    2426    3168    3253    4324 
   Size:        399     502      51      21      62     742      85    1071

Cuts at:   4324    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     502     399      85      62      51
                  21
 
BspEI T'CCGG_A

Cuts at:   4770    4770 
   Size:       5107
 
BspHI T'CATG_A

Cuts at:   2996    4726    2996 
   Size:       1730    3377
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2175    2462     844 
   Size:       1331     287    3489

  Fragments arranged by size: 

                3489    1331     287
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2994    1394 
   Size:        429    1171    3507

  Fragments arranged by size: 

                3507    1171     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3602    3776      65 
   Size:       1183    2354     174    1396

  Fragments arranged by size: 

                2354    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3751    3938    1700 
   Size:        110    1941     187    2869

  Fragments arranged by size: 

                2869    1941     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5107
 
BssHII G'CGCG_C

Cuts at:   1925    2028    1925 
   Size:        103    5004
 
BssSI C'ACGA_G

Cuts at:   3167    4179    3167 
   Size:       1012    4095
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5107
 
BstBI TT'CG_AA

Cuts at:   3911    3911 
   Size:       5107
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5107
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2353    2353 
   Size:       5107
 
BstYI r'GATC_y

Cuts at:   1972    2120    2330    2437    2804    3189    3206    4658    4744 
   Size:        148     210     107     367     385      17    1452      86

Cuts at:   4744    4756    5102    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     210     148     107
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2115    2115 
   Size:       5107
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2364     513 
   Size:        552     939     360    3256

  Fragments arranged by size: 

                3256     939     552     360
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4368

  Fragments arranged by size: 

                4368     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2364    2623    3417    3437    2364 
   Size:        259     794      20    4034

  Fragments arranged by size: 

                4034     794     259      20
 
ClaI AT'CG_AT

Cuts at:   1554    2217    1554 
   Size:        663    4444
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5072
 
DraI TTT'AAA

Cuts at:   2411    2411 
   Size:       5107
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4114     809 
   Size:        507    2798    1802

  Fragments arranged by size: 

                2798    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2078    2554    3443 
   Size:         54    1425      50     275      18     248     476     889

Cuts at:   3443    5072       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     476     275     248      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2078    2554    1537 
   Size:        275     266     476    4090

  Fragments arranged by size: 

                4090     476     275     266
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2298    3037    1353 
   Size:        945     739    3423

  Fragments arranged by size: 

                3423     945     739
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2324    3694    4068    4214     387 
   Size:       1064     138     735    1370     374     146    1280

  Fragments arranged by size: 

                1370    1280    1064     735     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2252    2291    3170    3767    4554    4822    1239 
   Size:        393     620      39     879     597     787     268    1524

  Fragments arranged by size: 

                1524     879     787     620     597     393     268      39
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2203    2203 
   Size:       5107
 
EcoO109I rG'GnC_Cy

Cuts at:   2383    2383 
   Size:       5107
 
EcoRI G'AATT_C

Cuts at:   2416    2794    2416 
   Size:        378    4729
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2257    2257 
   Size:       5107
 
FspI TGC'GCA

Cuts at:   3613    3613 
   Size:       5107
 
HaeII r_GCGC'y

Cuts at:   1678    4254    1678 
   Size:       2576    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3748    3780    3822    3854    1373 
   Size:         32     135      32    2176      32      42      32    2626

  Fragments arranged by size: 

                2626    2176     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2451    2697     669 
   Size:        988     281     513     246    3079

  Fragments arranged by size: 

                3079     988     513     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5107
 
HpaI GTT'AAC

Cuts at:   2697    2697 
   Size:       5107
 
KpnI G_GTAC'C

Cuts at:   2432    2432 
   Size:       5107
 
MfeI C'AATT_G

Cuts at:   2706    2706 
   Size:       5107
 
MluI A'CGCG_T

Cuts at:   4983    4983 
   Size:       5107
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4221    4405    1305 
   Size:       2916     184    2007

  Fragments arranged by size: 

                2916    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3285

  Fragments arranged by size: 

                3285    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2369    3065    3424    3583     518 
   Size:        648    1203     696     359     159    2042

  Fragments arranged by size: 

                2042    1203     696     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2190    2278    2321    2523    3200    4348    4593    1606 
   Size:        584      88      43     202     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     584     245     202      88      43
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3616

  Fragments arranged by size: 

                3616     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5107
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5107
 
NotI GC'GGCC_GC

Cuts at:   2554    2554 
   Size:       5107
 
NspI r_CATG'y

Cuts at:   1132    1229    2465    4010    1132 
   Size:         97    1236    1545    2229

  Fragments arranged by size: 

                2229    1545    1236      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5107
 
PciI A'CATG_T

Cuts at:   4006    4006 
   Size:       5107
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5107
 
PmeI GTTT'AAAC

Cuts at:   2411    2411 
   Size:       5107
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3176    3208    4689    4721    3176 
   Size:         32    1481      32    3562

  Fragments arranged by size: 

                3562    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2383    2383 
   Size:       5107
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5107
 
PsiI TTA'TAA

Cuts at:   2677    2677 
   Size:       5107
 
PstI C_TGCA'G

Cuts at:    830    2459     830 
   Size:       1629    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5107
 
PvuII CAG'CTG

Cuts at:   2278    2278 
   Size:       5107
 
SalI G'TCGA_C

Cuts at:   1936    2449    1936 
   Size:        513    4594
 
SbfI CC_TGCA'GG

Cuts at:   2459    2459 
   Size:       5107
 
ScaI AGT'ACT

Cuts at:   3355    5067    3355 
   Size:       1712    3395
 
SfcI C'TryA_G

Cuts at:    826    1045    2293    2455    3590    4271    4462     826 
   Size:        219    1248     162    1135     681     191    1471

  Fragments arranged by size: 

                1471    1248    1135     681     219     191     162
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5107
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5107
 
SmaI CCC'GGG

Cuts at:   1872    2434    1872 
   Size:        562    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3211    4112    4374     820 
   Size:        197     926    1268     901     262    1553

  Fragments arranged by size: 

                1553    1268     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2898     493 
   Size:       2405    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5107
 
SphI G_CATG'C

Cuts at:   1132    2465    1132 
   Size:       1333    3774
 
SspI AAT'ATT

Cuts at:      5      52    3031       5 
   Size:         47    2979    2081

  Fragments arranged by size: 

                2979    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5107
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2546     513 
   Size:        552     939     542    3074

  Fragments arranged by size: 

                3074     939     552     542
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3134    3151    3304    3489    3934     325 
   Size:       1255    1554      17     153     185     445    1498

  Fragments arranged by size: 

                1554    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3353    5065      96 
   Size:        274      80      33      51    1443    1376    1712     138

  Fragments arranged by size: 

                1712    1443    1376     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2231    2263    2322    2354    5067    5099    2231 
   Size:         32      59      32    2713      32    2239

  Fragments arranged by size: 

                2713    2239      59      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5107
 
XbaI T'CTAG_A

Cuts at:   2443    2443 
   Size:       5107
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5107
 
XmaI C'CCGG_G

Cuts at:   1870    2432    1870 
   Size:        562    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3236    3236 
   Size:       5107
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2915     276 
   Size:         53      83     186     936    1381    2468

  Fragments arranged by size: 

                2468    1381     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI
     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   DraIII
  EcoICRI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com