Restriction map for
pFN21AF0343
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# NO CUT ENZYMES ( 54 enzymes) #
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AccIII AflII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AviII
BciVI BfrI BseAI BsiWI BspEI
BssHII CpoI DraI Eam1105I Eco47III
EcoRV EcoT22I FseI FspI HindIII
HpaI KspI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI PacI PinAI PmeI
PshBI RsrII SacI SacII SbfI
SgrAI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I XbaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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Acc65I 4587 GGTACC G^GTACC
AclI 6439 AACGTT AA^CGTT
AcsI 6748 GAATTC [GA]^AATT[CT]
ApoI 6748 GAATTC [GA]^AATT[CT]
Asp718I 4587 GGTACC G^GTACC
AspI 5350 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6672 CCTAGG C^CTAGG
BlnI 6672 CCTAGG C^CTAGG
BsaBI 5499 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 5774 GCATTC GAATGC(1/-1)
BspLU11I 3361 ACATGT A^CATGT
BstBI 1696 TTCGAA TT^CGAA
EcoRI 6748 GAATTC G^AATTC
KpnI 4587 GGTACC GGTAC^C
MamI 5499 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 1696 TTCGAA TT^CGAA
PflFI 5350 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4006 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6439 AACGTT AA^CGTT
PvuI 7064 CGATCG CGAT^CG
SalI 61 GTCGAC G^TCGAC
SapI 7698 GAAGAGC GCTCTTC(1/4)
ScaI 4566 AGTACT AGT^ACT
SexAI 6032 ACCTGGT A^CC[AT]GGT
SfiI 3882 GGCCCGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1696 TTCGAA TT^CGAA
SnaBI 7687 TACGTA TAC^GTA
Tth111I 5350 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflIII 317 ACGTGT A^C[GA][CT]GT
3361 ACATGT
Asp700I 138 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6916 GAACACATTC
BbeI 595 GGCGCC GGCGC^C
3280 GGCGCC
BsaWI 3766 TCCGGT [AT]^CCGG[AT]
3862 ACCGGA
BspDI 4708 ATCGAT AT^CGAT
7066 ATCGAT
BsrBI 579 GAGCGG CCGCTC(-3/-3)
3511 GAGCGG
Bst1107I 2320 GTATAC GTA^TAC
4809 GTATAC
BstAPI 5033 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6030 GCACCTGGTGC
BstZ17I 2320 GTATAC GTA^TAC
4809 GTATAC
ClaI 4708 ATCGAT AT^CGAT
7066 ATCGAT
EcoNI 102 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
7830 CCTGCTCAAGG
KasI 595 GGCGCC G^GCGCC
3280 GGCGCC
NarI 595 GGCGCC GG^CGCC
3280 GGCGCC
PflMI 1658 CCATCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6057 CCAGCACGTGG
SfoI 595 GGCGCC GGC^GCC
3280 GGCGCC
Van91I 1658 CCATCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6057 CCAGCACGTGG
XmnI 138 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6916 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 159 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbrPI BbsI BbvCI BbvI BcgI
BclI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrFI
BsrGI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PleI PmaCI PmlI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI StuI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoI XhoII XmaCI XmaI