Restriction map for pFN21AF0343
 (Circular) (Six-base) MAPSORT of: pFN21AF0343.seq  Check: 5552  from: 1  to: 12622



pFN21AF0343  12622 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   5737    5737 
   Size:      12622
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4416    4731    9192   10432 
   Size:         53      83     186     936    2880     315    4461    1240

Cuts at:  10432     278 
   Size:       2468

  Fragments arranged by size: 

                4461    2880    2468    1240     936     315     186      83
                  53
 
AccI GT'mk_AC

Cuts at:   1937    2067    4326    6504    6815    9585    9720    9965    1937 
   Size:        130    2259    2178     311    2770     135     245    4594

  Fragments arranged by size: 

                4594    2770    2259    2178     311     245     135     130
            
 
Acc65I G'GTAC_C

Cuts at:   6592    9943    6592 
   Size:       3351    9271
 
AclI AA'CG_TT

Cuts at:   1563    8445   10749   11122    1563 
   Size:       6882    2304     373    3063

  Fragments arranged by size: 

                6882    3063    2304     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2195    2426    2551    2831    3569    4264    7997    8418 
   Size:        563     231     125     280     738     695    3733     421

Cuts at:   8418    8754   10685   12069   12337    1632 
   Size:        336    1931    1384     268    1917

  Fragments arranged by size: 

                3733    1931    1917    1384     738     695     563     421
                 336     280     268     231     125
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197   12425
 
AflIII A'CryG_T

Cuts at:   1203    2322    5366   11521   12498    1203 
   Size:       1119    3044    6155     977    1327

  Fragments arranged by size: 

                6155    3044    1327    1119     977
 
AgeI A'CCGG_T

Cuts at:  11453   11453 
   Size:      12622
 
AhdI GACnn_n'nnGTC

Cuts at:  11351   11351 
   Size:      12622
 
AleI CACnn'nnGTG

Cuts at:   1166    6680    9863    1166 
   Size:       5514    3183    3925

  Fragments arranged by size: 

                5514    3925    3183
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    6038    9761    1184 
   Size:       4854    3723    4045

  Fragments arranged by size: 

                4854    4045    3723
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4718    4750    7791    7823    4718 
   Size:         32    3041      32    9517

  Fragments arranged by size: 

                9517    3041      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   3236    6953    7249    8763   10365   11937    3236 
   Size:       3717     296    1514    1602    1572    3921

  Fragments arranged by size: 

                3921    3717    1602    1572    1514     296
 
ApaI G_GGCC'C

Cuts at:   2596    3569    3755    4643    5036    5124    5324    6188    6372 
   Size:        973     186     888     393      88     200     864     184

Cuts at:   6372    6474    6558    7297    7694    8240    8676    8717    8813 
   Size:        102      84     739     397     546     436      41      96

Cuts at:   8813    9628    2596 
   Size:        815    5590

  Fragments arranged by size: 

                5590     973     888     864     815     739     546     436
                 397     393     200     186     184     102      96      88
                  84      41
 
ApaLI G'TGCA_C

Cuts at:   3371    6937    7036    7045    9083   10679   11835    3371 
   Size:       3566      99       9    2038    1596    1156    4158

  Fragments arranged by size: 

                4158    3566    2038    1596    1156      99       9
 
ApoI r'AATT_y

Cuts at:   1493    8753    9931   10157   10309   12418    1493 
   Size:       7260    1178     226     152    2109    1697

  Fragments arranged by size: 

                7260    2109    1697    1178     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032   11176     160 
   Size:        872   10144    1606

  Fragments arranged by size: 

               10144    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      12622
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2022    2336    2623    2735    3047    3312    3442 
   Size:         73      79     314     287     112     312     265     130

Cuts at:   3442    4524    5038    5339    5396    5507    5560    5681    5850 
   Size:       1082     514     301      57     111      53     121     169

Cuts at:   5850    6072    6662    7830    7951    8769    9162    9628    9947 
   Size:        222     590    1168     121     818     393     466     319

Cuts at:   9947    1870 
   Size:       4545

  Fragments arranged by size: 

                4545    1168    1082     818     590     514     466     393
                 319     314     312     301     287     265     222     169
                 130     121     121     112     111      79      73      57
                  53
 
AvrII C'CTAG_G

Cuts at:   8677    8677 
   Size:      12622
 
BamHI G'GATC_C

Cuts at:   2529    4864    6760    9952   10319    2529 
   Size:       2335    1896    3192     367    4832

  Fragments arranged by size: 

                4832    3192    2335    1896     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2600    3716    5050    5285    6043 
   Size:        325     217     627     813    1116    1334     235     758

Cuts at:   6043    6107    6263    6518    6592    7457    8947    9203    9359 
   Size:         64     156     255      74     865    1490     256     156

Cuts at:   9359    9451    9916    9943   11398     618 
   Size:         92     465      27    1455    1842

  Fragments arranged by size: 

                1842    1490    1455    1334    1116     865     813     758
                 627     465     325     256     255     235     217     156
                 156      92      74      64      27
 
BanII G_rGCy'C

Cuts at:   1391    2596    2824    2883    3110    3183    3569    3755    4356 
   Size:       1205     228      59     227      73     386     186     601

Cuts at:   4356    4371    4608    4643    4883    5036    5124    5255    5324 
   Size:         15     237      35     240     153      88     131      69

Cuts at:   5324    5397    5857    6188    6372    6383    6474    6558    6605 
   Size:         73     460     331     184      11      91      84      47

Cuts at:   6605    6669    7297    7694    7968    8220    8240    8431    8654 
   Size:         64     628     397     274     252      20     191     223

Cuts at:   8654    8676    8695    8717    8813    9103    9628    9941    1391 
   Size:         22      19      22      96     290     525     313    4072

  Fragments arranged by size: 

                4072    1205     628     601     525     460     397     386
                 331     313     290     274     252     240     237     228
                 227     223     191     186     184     153     131      96
                  91      88      84      73      73      69      64      59
                  47      35      22      22      20      19      15      11
            
 
BbeI G_GCGC'C

Cuts at:   2604    5289    2604 
   Size:       2685    9937
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3966    4061    5806    6788     928 
   Size:        971    2067      95    1745     982    6762

  Fragments arranged by size: 

                6762    2067    1745     982     971      95
 
BbvCI CC'TCA_GC

Cuts at:   3300    3971    4916    8465    3300 
   Size:        671     945    3549    7457

  Fragments arranged by size: 

                7457    3549     945     671
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2176    2210    2371    2405    3682    3716    4645 
   Size:         34     239      34     161      34    1277      34     929

Cuts at:   4645    4679   10813   10847    1903 
   Size:         34    6134      34    3678

  Fragments arranged by size: 

                6134    3678    1277     929     239     161      34      34
                  34      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:  10514   11724   10514 
   Size:       1210   11412
 
BclI T'GATC_A

Cuts at:   1548    2727    6993    8094    1548 
   Size:       1179    4266    1101    6076

  Fragments arranged by size: 

                6076    4266    1179    1101
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3455    5894    8526 
   Size:        107     122      71     631     605    1783    2439    2632

Cuts at:   8526    9065    9423   11233     136 
   Size:        539     358    1810    1525

  Fragments arranged by size: 

                2632    2439    1810    1783    1525     631     605     539
                 358     122     107      71
 
BglII A'GATC_T

Cuts at:   3219    5592    8826   12617    3219 
   Size:       2373    3234    3791    3224

  Fragments arranged by size: 

                3791    3234    3224    2373
 
BlpI GC'TnA_GC

Cuts at:   2255    8219    9680   10039    2255 
   Size:       5964    1461     359    4838

  Fragments arranged by size: 

                5964    4838    1461     359
 
Bme1580I G_kGCm'C

Cuts at:   1790    2596    2623    3290    3375    3396    3569    3719    3755 
   Size:        806      27     667      85      21     173     150      36

Cuts at:   3755    3827    4215    4438    4643    5036    5055    5124    5157 
   Size:         72     388     223     205     393      19      69      33

Cuts at:   5157    5324    5388    5727    6027    6048    6188    6372    6474 
   Size:        167      64     339     300      21     140     184     102

Cuts at:   6474    6543    6558    6918    6941    7040    7049    7176    7297 
   Size:         69      15     360      23      99       9     127     121

Cuts at:   7297    7694    8019    8240    8606    8676    8717    8813    9087 
   Size:        397     325     221     366      70      41      96     274

Cuts at:   9087    9364    9419    9613    9628    9921   10683   11839    1790 
   Size:        277      55     194      15     293     762    1156    2573

  Fragments arranged by size: 

                2573    1156     806     762     667     397     393     388
                 366     360     339     325     300     293     277     274
                 223     221     205     194     184     173     167     150
                 140     127     121     102      99      96      85      72
                  70      69      69      64      55      41      36      33
                  27      23      21      21      19      15      15       9
            
 
BmgBI CAC'GTC

Cuts at:   1318    3376    3820    5881    6001    6499    6838    7035    7078 
   Size:       2058     444    2061     120     498     339     197      43

Cuts at:   7078    7807    8283    1318 
   Size:        729     476    5657

  Fragments arranged by size: 

                5657    2061    2058     729     498     476     444     339
                 197     120      43
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2650    2785    3177 
   Size:        416      53      83     399      24    1233     135     392

Cuts at:   3177   10353   11311     442 
   Size:       7176     958    1753

  Fragments arranged by size: 

                7176    1753    1233     958     416     399     392     135
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:      12622
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5614    6205    5614 
   Size:        591   12031
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3197    3326    3458    3782    4461    5534    8291    8744 
   Size:       1958     129     132     324     679    1073    2757     453

Cuts at:   8744    8760    8805    9125   11282    1239 
   Size:         16      45     320    2157    2579

  Fragments arranged by size: 

                2757    2579    2157    1958    1073     679     453     324
                 320     132     129      45      16
 
Bpu10I CC'TnA_GC

Cuts at:   2216    3300    3971    4916    5500    6377    8465    2216 
   Size:       1084     671     945     584     877    2088    6373

  Fragments arranged by size: 

                6373    2088    1084     945     877     671     584
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2336    4340    9824   10747   11612   11910    2336 
   Size:       2004    5484     923     865     298    3048

  Fragments arranged by size: 

                5484    3048    2004     923     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2918    5915    6134    7245    8140   11285     882 
   Size:        617    1419    2997     219    1111     895    3145    2219

  Fragments arranged by size: 

                3145    2997    2219    1419    1111     895     617     219
            
 
BsaAI yAC'GTr

Cuts at:    493    1204    2962    3124    3669    3943    4037    4210    5383 
   Size:        711    1758     162     545     274      94     173    1173

Cuts at:   5383    5464    6813    6829    7171    7999    8068    8353    8641 
   Size:         81    1349      16     342     828      69     285     288

Cuts at:   8641    9694    9805   10413     493 
   Size:       1053     111     608    2702

  Fragments arranged by size: 

                2702    1758    1349    1173    1053     828     711     608
                 545     342     288     285     274     173     162     111
                  94      81      69      16
 
BsaBI GATnn'nnATC

Cuts at:   7508   12616    7508 
   Size:       5108    7514
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2064    2079    2601    2655 
   Size:         53      83     186     936     531      15     522      54

Cuts at:   2655    3144    3360    3771    4257    4413    4617    4728    4842 
   Size:        489     216     411     486     156     204     111     114

Cuts at:   4842    5286    5847    6726    6927    8422    8973    9189    9648 
   Size:        444     561     879     201    1495     551     216     459

Cuts at:   9648   10429   10811   11446     275 
   Size:        781     382     635    1451

  Fragments arranged by size: 

                1495    1451     936     879     781     635     561     551
                 531     522     489     486     459     444     411     382
                 216     216     204     201     186     156     114     111
                  83      54      53      15
 
BsaWI w'CCGG_w

Cuts at:   5771    5867   11055   11453   11727   11874   12285    5771 
   Size:         96    5188     398     274     147     411    6108

  Fragments arranged by size: 

                6108    5188     411     398     274     147      96
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2438    2468    4187    4217    4935 
   Size:         30     165      30    1035      30    1719      30     718

Cuts at:   4935    4965    9371    9401    9455    9485    1178 
   Size:         30    4406      30      54      30    4315

  Fragments arranged by size: 

                4406    4315    1719    1035     718     165      54      30
                  30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2489    2492    2501    2753    3002    3593    5993    6998 
   Size:       1303       3       9     252     249     591    2400    1005

Cuts at:   6998    7976    9122    9800    1186 
   Size:        978    1146     678    4008

  Fragments arranged by size: 

                4008    2400    1303    1146    1005     978     678     591
                 252     249       9       3
 
BseYI C'CCAG_C

Cuts at:   2710    3829    5528    5735    6026    6542    7125    7205    7481 
   Size:       1119    1699     207     291     516     583      80     276

Cuts at:   7481    8240    8708    8813    9319    9615   11825   12321    2710 
   Size:        759     468     105     506     296    2210     496    3011

  Fragments arranged by size: 

                3011    2210    1699    1119     759     583     516     506
                 496     468     296     291     276     207     105      80
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2426    5064    5972    7244    7481    7699    8305 
   Size:        573     690    2638     908    1272     237     218     606

Cuts at:   8305    1163 
   Size:       5480

  Fragments arranged by size: 

                5480    2638    1272     908     690     606     573     237
                 218
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2590    2976    5619    6240    9072   10072 
   Size:        275     186     589     386    2643     621    2832    1000

Cuts at:  10072   10833   11861    1540 
   Size:        761    1028    2301

  Fragments arranged by size: 

                2832    2643    2301    1028    1000     761     621     589
                 386     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2172    2256    2769    3081    3375    5501    6365    6941    7040 
   Size:         84     513     312     294    2126     864     576      99

Cuts at:   7040    7049    7170    8037    9087    9888   10683   10768   11839 
   Size:          9     121     867    1050     801     795      85    1071

Cuts at:  11839    2172 
   Size:       2955

  Fragments arranged by size: 

                2955    2126    1071    1050     867     864     801     795
                 576     513     312     294     121      99      85      84
                   9
 
BsmI GAATG_Cn'

Cuts at:   7779   10133   10226    7779 
   Size:       2354      93   10175

  Fragments arranged by size: 

               10175    2354      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   6980    7904    8018    9545    6980 
   Size:        924     114    1527   10057

  Fragments arranged by size: 

               10057    1527     924     114
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2172    2256    2596    2623    2769    2824    2883 
   Size:        399     382      84     340      27     146      55      59

Cuts at:   2883    3081    3110    3183    3290    3375    3396    3569    3719 
   Size:        198      29      73     107      85      21     173     150

Cuts at:   3719    3755    3827    4215    4356    4371    4438    4608    4643 
   Size:         36      72     388     141      15      67     170      35

Cuts at:   4643    4883    5036    5055    5124    5157    5255    5324    5388 
   Size:        240     153      19      69      33      98      69      64

Cuts at:   5388    5397    5501    5727    5857    6027    6048    6188    6365 
   Size:          9     104     226     130     170      21     140     177

Cuts at:   6365    6372    6383    6474    6543    6558    6605    6669    6918 
   Size:          7      11      91      69      15      47      64     249

Cuts at:   6918    6941    7040    7049    7170    7176    7297    7694    7968 
   Size:         23      99       9     121       6     121     397     274

Cuts at:   7968    8019    8037    8220    8240    8431    8606    8654    8676 
   Size:         51      18     183      20     191     175      48      22

Cuts at:   8676    8695    8717    8813    9087    9103    9364    9419    9613 
   Size:         19      22      96     274      16     261      55     194

Cuts at:   9613    9628    9888    9921    9941   10683   10768   11839    1391 
   Size:         15     260      33      20     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     397     388     382     340
                 274     274     261     260     249     240     226     198
                 194     191     183     177     175     173     170     170
                 153     150     146     141     140     130     121     121
                 107     104      99      98      96      91      85      85
                  84      73      72      69      69      69      67      64
                  64      59      55      55      51      48      47      36
                  35      33      33      29      27      23      22      22
                  21      21      20      20      19      19      18      16
                  15      15      15      11       9       9       7       6
            
 
BspEI T'CCGG_A

Cuts at:  12285   12285 
   Size:      12622
 
BspHI T'CATG_A

Cuts at:   2775    4612    6631   10511   12241    2775 
   Size:       1837    2019    3880    1730    3156

  Fragments arranged by size: 

                3880    3156    2019    1837    1730
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2211    5737    5764    6445    7761    9281    9843    9977 
   Size:       1367    3526      27     681    1316    1520     562     134

Cuts at:   9977     844 
   Size:       3489

  Fragments arranged by size: 

                3526    3489    1520    1367    1316     681     562     134
                  27
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2586    5518   10509    1394 
   Size:        429     763    2932    4991    3507

  Fragments arranged by size: 

                4991    3507    2932     763     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4192    4495    6449    8796    9352   11117   11291 
   Size:       1183    2944     303    1954    2347     556    1765     174

Cuts at:  11291      65 
   Size:       1396

  Fragments arranged by size: 

                2944    2347    1954    1765    1396    1183     556     303
                 174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2089    3386    4222    5288    9813   11266   11453 
   Size:        110     279    1297     836    1066    4525    1453     187

Cuts at:  11453    1700 
   Size:       2869

  Fragments arranged by size: 

                4525    2869    1453    1297    1066     836     279     187
                 110
 
BsrGI T'GTAC_A

Cuts at:     96    3519    5625    5952      96 
   Size:       3423    2106     327    6766

  Fragments arranged by size: 

                6766    3423    2106     327
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:      12622
 
BssSI C'ACGA_G

Cuts at:   4364    7499    9340    9563   10682   11694    4364 
   Size:       3135    1841     223    1119    1012    5292

  Fragments arranged by size: 

                5292    3135    1841    1119    1012     223
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    7044    8041     794 
   Size:       6250     997    5375

  Fragments arranged by size: 

                6250    5375     997
 
BstBI TT'CG_AA

Cuts at:   3702   11426    3702 
   Size:       7724    4898
 
BstEII G'GTnAC_C

Cuts at:   1184    3553    5137    8290    8512    9370    1184 
   Size:       2369    1584    3153     222     858    4436

  Fragments arranged by size: 

                4436    3153    2369    1584     858     222
 
BstXI CCAn_nnnn'nTGG

Cuts at:   5029    5441    5645    5786    6481    8089    8248    9215    9658 
   Size:        412     204     141     695    1608     159     967     443

Cuts at:   9658    9673    5029 
   Size:         15    7978

  Fragments arranged by size: 

                7978    1608     967     695     443     412     204     159
                 141      15
 
BstYI r'GATC_y

Cuts at:   1972    2133    2529    3219    4864    5592    6760    6882    7915 
   Size:        161     396     690    1645     728    1168     122    1033

Cuts at:   7915    8826    9231    9789    9952   10319   10704   10721   12173 
   Size:        911     405     558     163     367     385      17    1452

Cuts at:  12173   12259   12271   12617    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1645    1452    1168    1033     911     728     690
                 558     405     396     385     367     346     163     161
                 122      86      17      12
 
BstZ17I GTA'TAC

Cuts at:   4327    6816    4327 
   Size:       2489   10133
 
Bsu36I CC'TnA_GG

Cuts at:   5905    7343    7414    9094    9420    5905 
   Size:       1438      71    1680     326    9107

  Fragments arranged by size: 

                9107    1680    1438     326      71
 
BtgI C'CryG_G

Cuts at:    513    1065    2123    3273    3351    3636    4239    5638    5938 
   Size:        552    1058    1150      78     285     603    1399     300

Cuts at:   5938    5994    6085    6699    7609    8667    9447     513 
   Size:         56      91     614     910    1058     780    3688

  Fragments arranged by size: 

                3688    1399    1150    1058    1058     910     780     614
                 603     552     300     285      91      78      56
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2179    3082    3178    3837    5958    8214 
   Size:        683      56     953     903      96     659    2121    2256

Cuts at:   8214     487 
   Size:       4895

  Fragments arranged by size: 

                4895    2256    2121     953     903     683     659      96
                  56
 
BtsI GCAGTG_nn'

Cuts at:   3544    4767    5251    7584    7824   10138   10932   10952    3544 
   Size:       1223     484    2333     240    2314     794      20    5214

  Fragments arranged by size: 

                5214    2333    2314    1223     794     484     240      20
            
 
ClaI AT'CG_AT

Cuts at:   1554    6714    9072    1554 
   Size:       5160    2358    5104

  Fragments arranged by size: 

                5160    5104    2358
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    3196    3231    4090    4125    6853    6888     529 
   Size:         35    2632      35     859      35    2728      35    6263

  Fragments arranged by size: 

                6263    2728    2632     859      35      35      35      35
            
 
DraI TTT'AAA

Cuts at:   9926    9926 
   Size:      12622
 
DraIII CAC_nnn'GTG

Cuts at:   3140    3370    3723    5995    7044    8041    3140 
   Size:        230     353    2272    1049     997    7721

  Fragments arranged by size: 

                7721    2272    1049     997     353     230
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2785    6346    8897   11629     809 
   Size:        507    1469    3561    2551    2732    1802

  Fragments arranged by size: 

                3561    2732    2551    1802    1469     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2091    2587    2938 
   Size:         54    1425      50     275      18     261     496     351

Cuts at:   2938    2973    3088    3427    3463    3911    4065    4402    5616 
   Size:         35     115     339      36     448     154     337    1214

Cuts at:   5616    5713    5895    6292    7279    7576    7680    7932    7993 
   Size:         97     182     397     987     297     104     252      61

Cuts at:   7993    8059    8070    8439    8793    9663   10069   10958   12587 
   Size:         66      11     369     354     870     406     889    1629

Cuts at:  12587       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1214     987     889     870     496     448
                 406     397     369     354     351     339     337     297
                 275     261     252     182     154     115     104      97
                  66      61      54      50      43      36      35      18
                  11
 
EagI C'GGCC_G

Cuts at:   1537    1812    2587    2973    5616   10069    1537 
   Size:        275     775     386    2643    4453    4090

  Fragments arranged by size: 

                4453    4090    2643     775     386     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    5426    6582    9698   10552    1353 
   Size:       4073    1156    3116     854    3423

  Fragments arranged by size: 

                4073    3423    3116    1156     854
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2126    2291    2714    3956   11209   11583 
   Size:       1064     138     537     165     423    1242    7253     374

Cuts at:  11583   11729     387 
   Size:        146    1280

  Fragments arranged by size: 

                7253    1280    1242    1064     537     423     374     165
                 146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2195    2426    2551    2831    3197    3326    3458 
   Size:        393     563     231     125     280     366     129     132

Cuts at:   3458    3569    3782    4264    4461    5534    7997    8291    8418 
   Size:        111     213     482     197    1073    2463     294     127

Cuts at:   8418    8744    8754    8760    8805    9125   10685   11282   12069 
   Size:        326      10       6      45     320    1560     597     787

Cuts at:  12069   12337    1239 
   Size:        268    1524

  Fragments arranged by size: 

                2463    1560    1524    1073     787     597     563     482
                 393     366     326     320     294     280     268     231
                 213     197     132     129     127     125     111      45
                  10       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2111    9839    2111 
   Size:       7728    4894
 
EcoO109I rG'GnC_Cy

Cuts at:   2104    2593    2968    3167    3268    3469    3565    3566    3598 
   Size:        489     375     199     101     201      96       1      32

Cuts at:   3598    3751    3752    4117    4451    4639    4640    5033    5120 
   Size:        153       1     365     334     188       1     393      87

Cuts at:   5120    5121    5320    5321    5504    5685    6184    6185    6322 
   Size:          1     199       1     183     181     499       1     137

Cuts at:   6322    6470    6554    6555    6736    7294    7305    7347    7669 
   Size:        148      84       1     181     558      11      42     322

Cuts at:   7669    7690    8146    8236    8237    8266    8672    8673    8713 
   Size:         21     456      90       1      29     406       1      40

Cuts at:   8713    8714    8809    8810    9262    9533    9624    9625    9632 
   Size:          1      95       1     452     271      91       1       7

Cuts at:   9632    9670    9774    2104 
   Size:         38     104    4952

  Fragments arranged by size: 

                4952     558     499     489     456     452     406     393
                 375     365     334     322     271     201     199     199
                 188     183     181     181     153     148     137     104
                 101      96      95      91      90      87      84      42
                  40      38      32      29      21      11       7       1
                   1       1       1       1       1       1       1       1
                   1       1       1
 
EcoRI G'AATT_C

Cuts at:   8753    9931   10309    8753 
   Size:       1178     378   11066

  Fragments arranged by size: 

               11066    1178     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3127    9727    3127 
   Size:       6600    6022
 
FspI TGC'GCA

Cuts at:  11128   11128 
   Size:      12622
 
HaeII r_GCGC'y

Cuts at:   1678    2336    2604    3534    5289   11769    1678 
   Size:        658     268     930    1755    6480    2531

  Fragments arranged by size: 

                6480    2531    1755     930     658     268
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2309    2341    2630    2662    2753 
   Size:         32     135      32     737      32     289      32      91

Cuts at:   2753    2785    3046    3078    6745    6777    7202    7234    7679 
   Size:         32     261      32    3667      32     425      32     445

Cuts at:   7679    7711    9371    9403    9557    9589   11263   11295   11337 
   Size:         32    1660      32     154      32    1674      32      42

Cuts at:  11337   11369    1373 
   Size:         32    2626

  Fragments arranged by size: 

                3667    2626    1674    1660     737     445     425     289
                 261     154     135      91      42      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2068    4072    7570    7645    9022    9966 
   Size:        988     281     130    2004    3498      75    1377     944

Cuts at:   9966   10212     669 
   Size:        246    3079

  Fragments arranged by size: 

                3498    3079    2004    1377     988     944     281     246
                 130      75
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:      12622
 
HpaI GTT'AAC

Cuts at:  10212   10212 
   Size:      12622
 
KasI G'GCGC_C

Cuts at:   2600    5285    2600 
   Size:       2685    9937
 
KpnI G_GTAC'C

Cuts at:   6596    9947    6596 
   Size:       3351    9271
 
MfeI C'AATT_G

Cuts at:  10221   10221 
   Size:      12622
 
MluI A'CGCG_T

Cuts at:  12498   12498 
   Size:      12622
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2226    8085   11736   11920    1305 
   Size:        921    5859    3651     184    2007

  Fragments arranged by size: 

                5859    3651    2007     921     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2940    3090    3429    4404    5897 
   Size:         54    1425     343    1108     150     339     975    1493

Cuts at:   5897    6294    7578    7682    7995    8061    8072    8441    8795 
   Size:        397    1284     104     313      66      11     369     354

Cuts at:   8795    9665      10 
   Size:        870    2967

  Fragments arranged by size: 

                2967    1493    1425    1284    1108     975     870     397
                 369     354     343     339     313     150     104      66
                  54      11
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2456    2462    4238    4547    5643    6680    7148 
   Size:        648    1290       6    1776     309    1096    1037     468

Cuts at:   7148    7856    8087    9863   10580   10939   11098     518 
   Size:        708     231    1776     717     359     159    2042

  Fragments arranged by size: 

                2042    1776    1776    1290    1096    1037     717     708
                 648     468     359     309     231     159       6
 
MspA1I CmG'CkG

Cuts at:   1606    2290    2551    2616    3304    3333    3506    4200    5048 
   Size:        684     261      65     688      29     173     694     848

Cuts at:   5048    5528    6231    7249    7713    8594    9323   10038   10715 
   Size:        480     703    1018     464     881     729     715     677

Cuts at:  10715   11863   12108    1606 
   Size:       1148     245    2120

  Fragments arranged by size: 

                2120    1148    1018     881     848     729     715     703
                 694     688     684     677     480     464     261     245
                 173      65      29
 
NaeI GCC'GGC

Cuts at:   2091    3388    5290    9815    2091 
   Size:       1297    1902    4525    4898

  Fragments arranged by size: 

                4898    4525    1902    1297
 
NarI GG'CG_CC

Cuts at:   2601    5286    2601 
   Size:       2685    9937
 
NcoI C'CATG_G

Cuts at:    513    1065    3273    3351    3636    4239    7609    9447     513 
   Size:        552    2208      78     285     603    3370    1838    3688

  Fragments arranged by size: 

                3688    3370    2208    1838     603     552     285      78
            
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:      12622
 
NgoMIV G'CCGG_C

Cuts at:   2089    3386    5288    9813    2089 
   Size:       1297    1902    4525    4898

  Fragments arranged by size: 

                4898    4525    1902    1297
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:      12622
 
NotI GC'GGCC_GC

Cuts at:  10069   10069 
   Size:      12622
 
NspI r_CATG'y

Cuts at:   1132    1229    5370    5953    7528    7740    8048    9748    9980 
   Size:         97    4141     583    1575     212     308    1700     232

Cuts at:   9980   11525    1132 
   Size:       1545    2229

  Fragments arranged by size: 

                4141    2229    1700    1575    1545     583     308     232
                 212      97
 
PasI CC'CwG_GG

Cuts at:   1348    2596    3755    7273    1348 
   Size:       1248    1159    3518    6697

  Fragments arranged by size: 

                6697    3518    1248    1159
 
PciI A'CATG_T

Cuts at:   5366   11521    5366 
   Size:       6155    6467
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3669    8068    1108 
   Size:       2561    4399    5662

  Fragments arranged by size: 

                5662    4399    2561
 
PfoI T'CCnGG_A

Cuts at:   2343    3264    4137    5631    8493    8877    2343 
   Size:        921     873    1494    2862     384    6088

  Fragments arranged by size: 

                6088    2862    1494     921     873     384
 
PmeI GTTT'AAAC

Cuts at:   9926    9926 
   Size:      12622
 
PmlI CAC'GTG

Cuts at:   3669    3943    4037    5464    7171    7999    8068    8353    8641 
   Size:        274      94    1427    1707     828      69     285     288

Cuts at:   8641    9805    3669 
   Size:       1164    6486

  Fragments arranged by size: 

                6486    1707    1427    1164     828     288     285     274
                  94      69
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:  10691   10723   12204   12236   10691 
   Size:         32    1481      32   11077

  Fragments arranged by size: 

               11077    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2104    2968    3167    3268    3598    6322    8266    9262    9632 
   Size:        864     199     101     330    2724    1944     996     370

Cuts at:   9632    9774    2104 
   Size:        142    4952

  Fragments arranged by size: 

                4952    2724    1944     996     864     370     330     199
                 142     101
 
PshAI GACnn'nnGTC

Cuts at:   1613    6015    1613 
   Size:       4402    8220
 
PsiI TTA'TAA

Cuts at:  10192   10192 
   Size:      12622
 
PspOMI G'GGCC_C

Cuts at:   2592    3565    3751    4639    5032    5120    5320    6184    6368 
   Size:        973     186     888     393      88     200     864     184

Cuts at:   6368    6470    6554    7293    7690    8236    8672    8713    8809 
   Size:        102      84     739     397     546     436      41      96

Cuts at:   8809    9624    2592 
   Size:        815    5590

  Fragments arranged by size: 

                5590     973     888     864     815     739     546     436
                 397     393     200     186     184     102      96      88
                  84      41
 
PspXI vC'TCGA_Gb

Cuts at:   2336    5339    5560    2336 
   Size:       3003     221    9398

  Fragments arranged by size: 

                9398    3003     221
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   8555    8587    8555 
   Size:         32   12590
 
PstI C_TGCA'G

Cuts at:    830    2208    3338    5238    5748    8179    9728    9974     830 
   Size:       1378    1130    1900     510    2431    1549     246    3478

  Fragments arranged by size: 

                3478    2431    1900    1549    1378    1130     510     246
            
 
PvuI CG_AT'CG

Cuts at:   2001    9072    2001 
   Size:       7071    5551
 
PvuII CAG'CTG

Cuts at:   2551    2616    3304    3506    4200    6231    7249    7713    9323 
   Size:         65     688     202     694    2031    1018     464    1610

Cuts at:   9323    2551 
   Size:       5850

  Fragments arranged by size: 

                5850    2031    1610    1018     694     688     464     202
                  65
 
SalI G'TCGA_C

Cuts at:   1936    2066    9964    1936 
   Size:        130    7898    4594

  Fragments arranged by size: 

                7898    4594     130
 
SanDI GG'GwC_CC

Cuts at:   9262    9262 
   Size:      12622
 
SapI GCTCTTCn'nnn_

Cuts at:   9698    9698 
   Size:      12622
 
SbfI CC_TGCA'GG

Cuts at:   9974    9974 
   Size:      12622
 
ScaI AGT'ACT

Cuts at:   6573   10870   12582    6573 
   Size:       4297    1712    6613

  Fragments arranged by size: 

                6613    4297    1712
 
SexAI A'CCwGG_T

Cuts at:   8037    8037 
   Size:      12622
 
SfcI C'TryA_G

Cuts at:    826    1045    2204    3334    5234    5744    6177    6268    6559 
   Size:        219    1159    1130    1900     510     433      91     291

Cuts at:   6559    7774    8175    9724    9820    9970   11105   11786   11977 
   Size:       1215     401    1549      96     150    1135     681     191

Cuts at:  11977     826 
   Size:       1471

  Fragments arranged by size: 

                1900    1549    1471    1215    1159    1135    1130     681
                 510     433     401     291     219     191     150      96
                  91
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   5894    5894 
   Size:      12622
 
SfoI GGC'GCC

Cuts at:   2602    5287    2602 
   Size:       2685    9937
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      12622
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:      12622
 
SmaI CCC'GGG

Cuts at:   1872    2625    3314    4526    5398    5509    5683    5852    6074 
   Size:        753     689    1212     872     111     174     169     222

Cuts at:   6074    6664    8771    9630    9949    1872 
   Size:        590    2107     859     319    4545

  Fragments arranged by size: 

                4545    2107    1212     872     859     753     689     590
                 319     222     174     169     111
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2315    2336    4355    5339    5560    9839 
   Size:        197     926     372      21    2019     984     221    4279

Cuts at:   9839   10726   11627   11889     820 
   Size:        887     901     262    1553

  Fragments arranged by size: 

                4279    2019    1553     984     926     901     887     372
                 262     221     197      21
 
SnaBI TAC'GTA

Cuts at:    493    9694   10413     493 
   Size:       9201     719    2702

  Fragments arranged by size: 

                9201    2702     719
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:      12622
 
SphI G_CATG'C

Cuts at:   1132    9980    1132 
   Size:       8848    3774
 
SrfI GCCC'GGGC

Cuts at:   5852    5852 
   Size:      12622
 
SspI AAT'ATT

Cuts at:      5      52   10546       5 
   Size:         47   10494    2081

  Fragments arranged by size: 

               10494    2081      47
 
StuI AGG'CCT

Cuts at:   1517    5073    6480    8790    9689    1517 
   Size:       3556    1407    2310     899    4450

  Fragments arranged by size: 

                4450    3556    2310    1407     899
 
StyI C'CwwG_G

Cuts at:    513    1065    2799    3163    3273    3351    3480    3570    3636 
   Size:        552    1734     364     110      78     129      90      66

Cuts at:   3636    4239    4749    5280    6351    7609    7650    8019    8677 
   Size:        603     510     531    1071    1258      41     369     658

Cuts at:   8677    9060    9078    9390    9447    9597    9666    9770   10061 
   Size:        383      18     312      57     150      69     104     291

Cuts at:  10061     513 
   Size:       3074

  Fragments arranged by size: 

                3074    1734    1258    1071     658     603     552     531
                 510     383     369     364     312     291     150     129
                 110     104      90      78      69      66      57      41
                  18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4032    4697    5447    6068    6226    6536    7304 
   Size:       1255    2452     665     750     621     158     310     768

Cuts at:   7304    7495    7622    9221   10649   10666   10819   11004   11449 
   Size:        191     127    1599    1428      17     153     185     445

Cuts at:  11449     325 
   Size:       1498

  Fragments arranged by size: 

                2452    1599    1498    1428    1255     768     750     665
                 621     445     310     191     185     158     153     127
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3519    4515    4812 
   Size:        274      80      33      51    1443    1542     996     297

Cuts at:   4812    5625    5952    5964    6243    6571    7260    7791    8625 
   Size:        813     327      12     279     328     689     531     834

Cuts at:   8625    9855   10868   12580      96 
   Size:       1230    1013    1712     138

  Fragments arranged by size: 

                1712    1542    1443    1230    1013     996     834     813
                 689     531     328     327     297     279     274     138
                  80      51      33      12
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2108    2140    4113    4145    4935    4967    8267    8299   12582 
   Size:         32    1973      32     790      32    3300      32    4283

Cuts at:  12582   12614    2108 
   Size:         32    2116

  Fragments arranged by size: 

                4283    3300    2116    1973     790      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    7358    1316 
   Size:       6042    6580
 
XbaI T'CTAG_A

Cuts at:   9958    9958 
   Size:      12622
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2890    6481    6697    8108    8354    8642    9397    9658    2890 
   Size:       3591     216    1411     246     288     755     261    5854

  Fragments arranged by size: 

                5854    3591    1411     755     288     261     246     216
            
 
XhoI C'TCGA_G

Cuts at:   1943    2336    5339    5560    1943 
   Size:        393    3003     221    9005

  Fragments arranged by size: 

                9005    3003     393     221
 
XmaI C'CCGG_G

Cuts at:   1870    2623    3312    4524    5396    5507    5681    5850    6072 
   Size:        753     689    1212     872     111     174     169     222

Cuts at:   6072    6662    8769    9628    9947    1870 
   Size:        590    2107     859     319    4545

  Fragments arranged by size: 

                4545    2107    1212     872     859     753     689     590
                 319     222     174     169     111
 
XmnI GAAnn'nnTTC

Cuts at:   2147    8925   10751    2147 
   Size:       6778    1826    4018

  Fragments arranged by size: 

                6778    4018    1826
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4414    4729    9190   10430 
   Size:         53      83     186     936    2880     315    4461    1240

Cuts at:  10430     276 
   Size:       2468

  Fragments arranged by size: 

                4461    2880    2468    1240     936     315     186      83
                  53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AscI     BaeI    BfrBI    BsiWI  EcoICRI    EcoRV     FseI
    FspAI     NruI     NsiI     PacI    RsrII     SacI    SacII     SwaI
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