Restriction map for
pFN21AF0267
(Linear) (Six-base) MAPSORT of: FLJ00267.seq Check: 1391 from: 1 to: 3303
FLJ00267.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 83 764 2889 3303
Size: 83 681 2125 414
Fragments arranged by size:
2125 681 414 83
AccI GT'mk_AC
Cuts at: 0 2224 3303
Size: 2224 1079
Acc65I G'GTAC_C
Cuts at: 0 2417 3303
Size: 2417 886
AclI AA'CG_TT
Cuts at: 0 1310 3303
Size: 1310 1993
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1814 2501 2606 3182 3303
Size: 1814 687 105 576 121
Fragments arranged by size:
1814 687 576 121 105
AfeI AGC'GCT
Cuts at: 0 376 3303
Size: 376 2927
AflIII A'CryG_T
Cuts at: 0 627 3303
Size: 627 2676
AhdI GACnn_n'nnGTC
Cuts at: 0 1503 3303
Size: 1503 1800
AleI CACnn'nnGTG
Cuts at: 0 628 3303
Size: 628 2675
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2907 3303
Size: 2907 396
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 398 430 3303
Size: 398 32 2873
Fragments arranged by size:
2873 398 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1561 1763 1932 2479 3303
Size: 1561 202 169 547 824
Fragments arranged by size:
1561 824 547 202 169
ApaI G_GGCC'C
Cuts at: 0 1040 2319 3303
Size: 1040 1279 984
Fragments arranged by size:
1279 1040 984
ApoI r'AATT_y
Cuts at: 0 124 205 3303
Size: 124 81 3098
Fragments arranged by size:
3098 124 81
AvaI C'yCGr_G
Cuts at: 0 704 721 2895 2945 3011 3303
Size: 704 17 2174 50 66 292
Fragments arranged by size:
2174 704 292 66 50 17
BamHI G'GATC_C
Cuts at: 0 306 3181 3303
Size: 306 2875 122
Fragments arranged by size:
2875 306 122
BanI G'GyrC_C
Cuts at: 0 96 2417 2817 2877 3303
Size: 96 2321 400 60 426
Fragments arranged by size:
2321 426 400 96 60
BanII G_rGCy'C
Cuts at: 0 522 1040 1667 2319 3155 3303
Size: 522 518 627 652 836 148
Fragments arranged by size:
836 652 627 522 518 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 2459 3303
Size: 2459 844
BbvCI CC'TCA_GC
Cuts at: 0 344 532 929 2336 2372 2534 2678 3303
Size: 344 188 397 1407 36 162 144 625
Fragments arranged by size:
1407 625 397 344 188 162 144 36
BfrBI ATG'CAT
Cuts at: 0 492 3262 3303
Size: 492 2770 41
Fragments arranged by size:
2770 492 41
BglI GCCn_nnn'nGGC
Cuts at: 0 1026 1189 2703 2753 2907 3303
Size: 1026 163 1514 50 154 396
Fragments arranged by size:
1514 1026 396 163 154 50
BlpI GC'TnA_GC
Cuts at: 0 1988 3303
Size: 1988 1315
Bme1580I G_kGCm'C
Cuts at: 0 101 480 1040 1292 1348 2319 2822 3303
Size: 101 379 560 252 56 971 503 481
Fragments arranged by size:
971 560 503 481 379 252 101 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 1144 2705 2843 3303
Size: 1144 1561 138 460
Fragments arranged by size:
1561 1144 460 138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1713 3303
Size: 1713 1590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1513 1678 1918 2015 2573 2629 2945
Size: 454 1059 165 240 97 558 56 316
Cuts at: 2945 3169 3303
Size: 224 134
Fragments arranged by size:
1059 558 454 316 240 224 165 134
97 56
Bpu10I CC'TnA_GC
Cuts at: 0 344 371 532 929 2336 2372 2534 2678
Size: 344 27 161 397 1407 36 162 144
Cuts at: 2678 3139 3303
Size: 461 164
Fragments arranged by size:
1407 461 397 344 164 162 161 144
36 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1360 1431 1530 3020 3303
Size: 1360 71 99 1490 283
Fragments arranged by size:
1490 1360 283 99 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 180 2032 2534 3303
Size: 180 1852 502 769
Fragments arranged by size:
1852 769 502 180
BsaAI yAC'GTr
Cuts at: 0 630 3303
Size: 630 2673
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 667 697 1513 1543 2401 2431
Size: 253 30 384 30 816 30 858 30
Cuts at: 2431 3303
Size: 872
Fragments arranged by size:
872 858 816 384 253 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 168 261 1675 1705 1816 2228 2353
Size: 148 20 93 1414 30 111 412 125
Cuts at: 2353 2501 2701 3119 3303
Size: 148 200 418 184
Fragments arranged by size:
1414 418 412 200 184 148 148 125
111 93 30 20
BseYI C'CCAG_C
Cuts at: 0 1003 1039 1171 1192 1756 1961 2083 3303
Size: 1003 36 132 21 564 205 122 1220
Fragments arranged by size:
1220 1003 564 205 132 122 36 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 1229 1537 3188 3303
Size: 140 1089 308 1651 115
Fragments arranged by size:
1651 1089 308 140 115
BsiHKAI G_wGCw'C
Cuts at: 0 74 833 1667 3155 3303
Size: 74 759 834 1488 148
Fragments arranged by size:
1488 834 759 148 74
BsmBI CGTCTCn'nnnn_
Cuts at: 0 273 3303
Size: 273 3030
Bsp1286I G_dGCh'C
Cuts at: 0 74 101 480 522 833 1040 1292 1348
Size: 74 27 379 42 311 207 252 56
Cuts at: 1348 1667 2319 2822 3155 3303
Size: 319 652 503 333 148
Fragments arranged by size:
652 503 379 333 319 311 252 207
148 74 56 42 27
BspHI T'CATG_A
Cuts at: 0 443 3303
Size: 443 2860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 239 536 764 2889 3242 3303
Size: 83 156 297 228 2125 353 61
Fragments arranged by size:
2125 353 297 228 156 83 61
BsrBI CCG'CTC
Cuts at: 0 45 219 2804 3303
Size: 45 174 2585 499
Fragments arranged by size:
2585 499 174 45
BsrDI GCAATG_nn'
Cuts at: 0 1060 2861 3303
Size: 1060 1801 442
Fragments arranged by size:
1801 1060 442
BsrFI r'CCGG_y
Cuts at: 0 323 1361 2906 3303
Size: 323 1038 1545 397
Fragments arranged by size:
1545 1038 397 323
BsrGI T'GTAC_A
Cuts at: 0 2355 3303
Size: 2355 948
BssSI C'ACGA_G
Cuts at: 0 1166 3303
Size: 1166 2137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 609 643 981 2446 2551 3303
Size: 609 34 338 1465 105 752
Fragments arranged by size:
1465 752 609 338 105 34
BstYI r'GATC_y
Cuts at: 0 155 306 386 3181 3303
Size: 155 151 80 2795 122
Fragments arranged by size:
2795 155 151 122 80
BstZ17I GTA'TAC
Cuts at: 0 2225 3303
Size: 2225 1078
Bsu36I CC'TnA_GG
Cuts at: 0 555 1121 1682 2699 3303
Size: 555 566 561 1017 604
Fragments arranged by size:
1017 604 566 561 555
BtgI C'CryG_G
Cuts at: 0 919 1332 1769 3303
Size: 919 413 437 1534
Fragments arranged by size:
1534 919 437 413
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 794 3303
Size: 794 2509
BtsI GCAGTG_nn'
Cuts at: 0 261 742 961 3303
Size: 261 481 219 2342
Fragments arranged by size:
2342 481 261 219
DraIII CAC_nnn'GTG
Cuts at: 0 994 1138 3303
Size: 994 144 2165
Fragments arranged by size:
2165 994 144
EaeI y'GGCC_r
Cuts at: 0 506 894 967 1051 1319 1363 2904 3303
Size: 506 388 73 84 268 44 1541 399
Fragments arranged by size:
1541 506 399 388 268 84 73 44
EarI CTCTTCn'nnn_
Cuts at: 0 1943 2012 2126 2144 2252 2564 2649 2651
Size: 1943 69 114 18 108 312 85 2
Cuts at: 2651 3303
Size: 652
Fragments arranged by size:
1943 652 312 114 108 85 69 18
2
EcoICRI GAG'CTC
Cuts at: 0 1665 3153 3303
Size: 1665 1488 150
Fragments arranged by size:
1665 1488 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1513 1678 1814 1918 2015 2501 2573
Size: 454 1059 165 136 104 97 486 72
Cuts at: 2573 2606 2629 2945 3169 3182 3303
Size: 33 23 316 224 13 121
Fragments arranged by size:
1059 486 454 316 224 165 136 121
104 97 72 33 23 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 280 1320 2964 3287 3303
Size: 280 1040 1644 323 16
Fragments arranged by size:
1644 1040 323 280 16
EcoO109I rG'GnC_Cy
Cuts at: 0 428 701 726 1036 1861 2301 2315 2942
Size: 428 273 25 310 825 440 14 627
Cuts at: 2942 3303
Size: 361
Fragments arranged by size:
825 627 440 428 361 310 273 25
14
HaeII r_GCGC'y
Cuts at: 0 378 3303
Size: 378 2925
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 931 963 1489 1513 1521 1545 1708 1740
Size: 931 32 526 24 8 24 163 32
Cuts at: 1740 2566 2598 3018 3050 3142 3174 3303
Size: 826 32 420 32 92 32 129
Fragments arranged by size:
931 826 526 420 163 129 92 32
32 32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 0 1254 2616 3303
Size: 1254 1362 687
Fragments arranged by size:
1362 1254 687
HindIII A'AGCT_T
Cuts at: 0 193 3303
Size: 193 3110
KpnI G_GTAC'C
Cuts at: 0 2421 3303
Size: 2421 882
MfeI C'AATT_G
Cuts at: 0 361 3303
Size: 361 2942
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 974 1409 2817 3303
Size: 206 768 435 1408 486
Fragments arranged by size:
1408 768 486 435 206
MscI TGG'CCA
Cuts at: 0 508 896 1321 3303
Size: 508 388 425 1982
Fragments arranged by size:
1982 508 425 388
MslI CAynn'nnrTG
Cuts at: 0 586 607 628 3303
Size: 586 21 21 2675
Fragments arranged by size:
2675 586 21 21
MspA1I CmG'CkG
Cuts at: 0 574 1003 1760 1780 3303
Size: 574 429 757 20 1523
Fragments arranged by size:
1523 757 574 429 20
NaeI GCC'GGC
Cuts at: 0 2908 3303
Size: 2908 395
NdeI CA'TA_TG
Cuts at: 0 3258 3303
Size: 3258 45
NgoMIV G'CCGG_C
Cuts at: 0 2906 3303
Size: 2906 397
NsiI A_TGCA'T
Cuts at: 0 494 3264 3303
Size: 494 2770 39
Fragments arranged by size:
2770 494 39
NspI r_CATG'y
Cuts at: 0 492 496 1961 2766 3303
Size: 492 4 1465 805 537
Fragments arranged by size:
1465 805 537 492 4
PasI CC'CwG_GG
Cuts at: 0 1627 1642 3283 3303
Size: 1627 15 1641 20
Fragments arranged by size:
1641 1627 20 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 515 1171 3303
Size: 515 656 2132
Fragments arranged by size:
2132 656 515
PfoI T'CCnGG_A
Cuts at: 0 2922 3184 3303
Size: 2922 262 119
Fragments arranged by size:
2922 262 119
PmlI CAC'GTG
Cuts at: 0 630 3303
Size: 630 2673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1288 1320 3303
Size: 1288 32 1983
Fragments arranged by size:
1983 1288 32
PpuMI rG'GwC_Cy
Cuts at: 0 428 701 726 1861 2301 2942 3303
Size: 428 273 25 1135 440 641 361
Fragments arranged by size:
1135 641 440 428 361 273 25
PshAI GACnn'nnGTC
Cuts at: 0 233 1847 2104 2723 3303
Size: 233 1614 257 619 580
Fragments arranged by size:
1614 619 580 257 233
PspOMI G'GGCC_C
Cuts at: 0 1036 2315 3303
Size: 1036 1279 988
Fragments arranged by size:
1279 1036 988
PstI C_TGCA'G
Cuts at: 0 80 773 1002 3303
Size: 80 693 229 2301
Fragments arranged by size:
2301 693 229 80
PvuII CAG'CTG
Cuts at: 0 1003 1760 3303
Size: 1003 757 1543
Fragments arranged by size:
1543 1003 757
SacI G_AGCT'C
Cuts at: 0 1667 3155 3303
Size: 1667 1488 148
Fragments arranged by size:
1667 1488 148
SanDI GG'GwC_CC
Cuts at: 0 701 2942 3303
Size: 701 2241 361
Fragments arranged by size:
2241 701 361
SapI GCTCTTCn'nnn_
Cuts at: 0 1943 3303
Size: 1943 1360
SfcI C'TryA_G
Cuts at: 0 76 350 423 769 998 1091 1354 3303
Size: 76 274 73 346 229 93 263 1949
Fragments arranged by size:
1949 346 274 263 229 93 76 73
SmaI CCC'GGG
Cuts at: 0 706 3013 3303
Size: 706 2307 290
Fragments arranged by size:
2307 706 290
SmlI C'TyrA_G
Cuts at: 0 1339 1446 1545 3035 3303
Size: 1339 107 99 1490 268
Fragments arranged by size:
1490 1339 268 107 99
SphI G_CATG'C
Cuts at: 0 496 1961 2766 3303
Size: 496 1465 805 537
Fragments arranged by size:
1465 805 537 496
StuI AGG'CCT
Cuts at: 0 286 553 1791 2901 3303
Size: 286 267 1238 1110 402
Fragments arranged by size:
1238 1110 402 286 267
StyI C'CwwG_G
Cuts at: 0 1316 1401 2042 2144 2439 3303
Size: 1316 85 641 102 295 864
Fragments arranged by size:
1316 864 641 295 102 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 44 671 1126 1557 2740 3303
Size: 44 627 455 431 1183 563
Fragments arranged by size:
1183 627 563 455 431 44
TatI w'GTAC_w
Cuts at: 0 2355 3061 3303
Size: 2355 706 242
Fragments arranged by size:
2355 706 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 253 285 667 699 3303
Size: 253 32 382 32 2604
Fragments arranged by size:
2604 382 253 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2290 3303
Size: 2290 1013
XmaI C'CCGG_G
Cuts at: 0 704 3011 3303
Size: 704 2307 292
Fragments arranged by size:
2307 704 292
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BfrBI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KpnI
MfeI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV
NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SanDI SapI SfcI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BglII BmgBI BmtI
BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BssHII
BstAPI BstBI BstEII ClaI CspCI DraI DrdI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HpaI
KasI MluI NarI NcoI NheI NotI NruI PacI
PciI PmeI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI
XmnI ZraI