(Circular) (Six-base) MAPSORT of: pFN21AB9891.seq Check: 9556 from: 1 to: 6106 pFN21AB9891 6106 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3170 3366 4169 5553 5821 1632 Size: 1538 196 803 1384 268 1917 Fragments arranged by size: 1917 1538 1384 803 268 196 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 2377 5005 5982 1203 Size: 1174 2628 977 1327 Fragments arranged by size: 2628 1327 1174 977 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 4835 4835 Size: 6106 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6106 AlwNI CAG_nnn'CTG Cuts at: 2816 3849 5421 2816 Size: 1033 1572 3501 Fragments arranged by size: 3501 1572 1033 ApaLI G'TGCA_C Cuts at: 3238 4163 5319 3238 Size: 925 1156 4025 Fragments arranged by size: 4025 1156 925 ApoI r'AATT_y Cuts at: 1493 2253 2923 3129 3170 3415 3641 3793 5902 Size: 760 670 206 41 245 226 152 2109 Cuts at: 5902 1493 Size: 1697 Fragments arranged by size: 2109 1697 760 670 245 226 206 152 41 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 3431 1870 Size: 73 1488 4545 Fragments arranged by size: 4545 1488 73 BamHI G'GATC_C Cuts at: 3374 3436 3803 3374 Size: 62 367 5677 Fragments arranged by size: 5677 367 62 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3108 3427 4882 618 Size: 325 217 627 1321 319 1455 1842 Fragments arranged by size: 1842 1455 1321 627 325 319 217 BanII G_rGCy'C Cuts at: 1391 3083 3425 1391 Size: 1692 342 4072 Fragments arranged by size: 4072 1692 342 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4297 4331 1903 Size: 34 2360 34 3678 Fragments arranged by size: 3678 2360 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2287 2365 3998 5208 2287 Size: 78 1633 1210 3185 Fragments arranged by size: 3185 1633 1210 78 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BfrBI ATG'CAT Cuts at: 2879 2879 Size: 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4717 136 Size: 107 122 71 631 605 3045 1525 Fragments arranged by size: 3045 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3153 6101 3153 Size: 2948 3158 BlpI GC'TnA_GC Cuts at: 3523 3523 Size: 6106 Bme1580I G_kGCm'C Cuts at: 1790 2547 3242 4167 5323 1790 Size: 757 695 925 1156 2573 Fragments arranged by size: 2573 1156 925 757 695 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3837 4795 442 Size: 416 53 83 399 24 2420 958 1753 Fragments arranged by size: 2420 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2100 2333 2517 2724 4766 1239 Size: 861 233 184 207 2042 2579 Fragments arranged by size: 2579 2042 861 233 207 184 Bpu10I CC'TnA_GC Cuts at: 2941 2941 Size: 6106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2207 2417 3379 4231 5096 5394 2207 Size: 210 962 852 865 298 2919 Fragments arranged by size: 2919 962 865 852 298 210 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2337 4769 882 Size: 617 838 2432 2219 Fragments arranged by size: 2432 2219 838 617 BsaAI yAC'GTr Cuts at: 493 1204 3897 493 Size: 711 2693 2702 Fragments arranged by size: 2702 2693 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3913 4295 4930 275 Size: 53 83 186 936 2380 382 635 1451 Fragments arranged by size: 2380 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4539 4937 5211 5358 5769 4539 Size: 398 274 147 411 4876 Fragments arranged by size: 4876 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2653 2683 2722 2752 3289 Size: 30 165 30 1250 30 39 30 537 Cuts at: 3289 3319 1178 Size: 30 3965 Fragments arranged by size: 3965 1250 537 165 39 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2097 2109 3021 1186 Size: 911 12 912 4271 Fragments arranged by size: 4271 912 911 12 BseYI C'CCAG_C Cuts at: 2050 2623 5309 5805 2050 Size: 573 2686 496 2351 Fragments arranged by size: 2686 2351 573 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3095 1163 Size: 573 1359 4174 Fragments arranged by size: 4174 1359 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3556 4317 5345 1540 Size: 275 186 1555 761 1028 2301 Fragments arranged by size: 2301 1555 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3242 4167 4252 5323 3242 Size: 925 85 1071 4025 Fragments arranged by size: 4025 1071 925 85 BsmI GAATG_Cn' Cuts at: 3617 3710 3617 Size: 93 6013 BsmBI CGTCTCn'nnnn_ Cuts at: 2351 2351 Size: 6106 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2547 3083 3242 3425 4167 4252 5323 Size: 399 757 536 159 183 742 85 1071 Cuts at: 5323 1391 Size: 2174 Fragments arranged by size: 2174 1071 757 742 536 399 183 159 85 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 2425 3995 5725 2425 Size: 1570 1730 2806 Fragments arranged by size: 2806 1730 1570 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2824 3461 844 Size: 1980 637 3489 Fragments arranged by size: 3489 1980 637 BsrBI CCG'CTC Cuts at: 1394 1823 3993 1394 Size: 429 2170 3507 Fragments arranged by size: 3507 2170 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4601 4775 65 Size: 1183 3353 174 1396 Fragments arranged by size: 3353 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4750 4937 1700 Size: 110 2940 187 2869 Fragments arranged by size: 2940 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 4166 5178 4166 Size: 1012 5094 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6106 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstYI r'GATC_y Cuts at: 1972 2559 3153 3374 3436 3803 4188 4205 5657 Size: 587 594 221 62 367 385 17 1452 Cuts at: 5657 5743 5755 6101 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 594 587 385 367 346 221 86 62 17 12 BtgI C'CryG_G Cuts at: 513 1065 2973 513 Size: 552 1908 3646 Fragments arranged by size: 3646 1908 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5367 Fragments arranged by size: 5367 683 56 BtsI GCAGTG_nn' Cuts at: 2142 3622 4416 4436 2142 Size: 1480 794 20 3812 Fragments arranged by size: 3812 1480 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3553 4442 6071 Size: 54 1425 50 275 18 1723 889 1629 Cuts at: 6071 8 Size: 43 Fragments arranged by size: 1723 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3553 1537 Size: 275 1741 4090 Fragments arranged by size: 4090 1741 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 2998 3186 4036 1353 Size: 683 962 188 850 3423 Fragments arranged by size: 3423 962 850 683 188 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2106 2565 4693 5067 5213 387 Size: 1064 138 517 459 2128 374 146 1280 Fragments arranged by size: 2128 1280 1064 517 459 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2333 2517 2724 3170 3366 4169 Size: 393 468 233 184 207 446 196 803 Cuts at: 4169 4766 5553 5821 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 803 787 597 468 446 393 268 233 207 196 184 EcoRI G'AATT_C Cuts at: 3415 3793 3415 Size: 378 5728 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3324 3324 Size: 6106 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 2404 5253 1678 Size: 726 2849 2531 Fragments arranged by size: 2849 2531 726 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2720 2752 4747 4779 4821 Size: 32 135 32 1148 32 1995 32 42 Cuts at: 4821 4853 1373 Size: 32 2626 Fragments arranged by size: 2626 1995 1148 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2962 3450 3696 669 Size: 988 281 1024 488 246 3079 Fragments arranged by size: 3079 1024 988 488 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 2962 3696 2962 Size: 734 5372 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 2439 3085 3705 2439 Size: 646 620 4840 Fragments arranged by size: 4840 646 620 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2842 3330 5220 5404 1305 Size: 1537 488 1890 184 2007 Fragments arranged by size: 2007 1890 1537 488 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4284 Fragments arranged by size: 4284 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2824 3248 3330 4064 4423 4582 518 Size: 648 1658 424 82 734 359 159 2042 Fragments arranged by size: 2042 1658 734 648 424 359 159 82 MspA1I CmG'CkG Cuts at: 1606 2069 3522 4199 5347 5592 1606 Size: 463 1453 677 1148 245 2120 Fragments arranged by size: 2120 1453 1148 677 463 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5554 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NsiI A_TGCA'T Cuts at: 2881 2881 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 2381 3403 3464 5009 1132 Size: 97 1152 1022 61 1545 2229 Fragments arranged by size: 2229 1545 1152 1022 97 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6106 PciI A'CATG_T Cuts at: 2377 5005 2377 Size: 2628 3478 PflMI CCAn_nnn'nTGG Cuts at: 1108 2672 1108 Size: 1564 4542 PfoI T'CCnGG_A Cuts at: 2671 2671 Size: 6106 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6106 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PstI C_TGCA'G Cuts at: 830 3458 830 Size: 2628 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 PvuII CAG'CTG Cuts at: 2069 2069 Size: 6106 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SapI GCTCTTCn'nnn_ Cuts at: 3186 3186 Size: 6106 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 3304 4354 6066 3304 Size: 1050 1712 3344 Fragments arranged by size: 3344 1712 1050 SexAI A'CCwGG_T Cuts at: 2327 2327 Size: 6106 SfcI C'TryA_G Cuts at: 826 1045 2898 3341 3454 4589 5270 5461 826 Size: 219 1853 443 113 1135 681 191 1471 Fragments arranged by size: 1853 1471 1135 681 443 219 191 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 3433 1872 Size: 1561 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 2432 3394 4210 5111 5373 Size: 197 926 243 246 962 816 901 262 Cuts at: 5373 820 Size: 1553 Fragments arranged by size: 1553 962 926 901 816 262 246 243 197 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 3464 1132 Size: 2332 3774 SspI AAT'ATT Cuts at: 5 52 4030 5 Size: 47 3978 2081 Fragments arranged by size: 3978 2081 47 StuI AGG'CCT Cuts at: 1517 2304 1517 Size: 787 5319 StyI C'CwwG_G Cuts at: 513 1065 3545 513 Size: 552 2480 3074 Fragments arranged by size: 3074 2480 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4133 4150 4303 4488 4933 325 Size: 1255 2553 17 153 185 445 1498 Fragments arranged by size: 2553 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2267 3302 4352 Size: 274 80 33 51 1443 290 1035 1050 Cuts at: 4352 6064 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1050 1035 290 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2105 2137 2722 2754 2994 3026 6066 6098 2105 Size: 32 585 32 240 32 3040 32 2113 Fragments arranged by size: 3040 2113 585 240 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 3431 1870 Size: 1561 4545 XmnI GAAnn'nnTTC Cuts at: 3155 4235 3155 Size: 1080 5026 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI