(Circular) (Six-base) MAPSORT of: pFN21AB9882.seq Check: 8306 from: 1 to: 6544 pFN21AB9882 6544 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4354 278 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3360 3887 1937 Size: 1423 527 4594 Fragments arranged by size: 4594 1423 527 Acc65I G'GTAC_C Cuts at: 3865 3865 Size: 6544 AclI AA'CG_TT Cuts at: 1563 4671 5044 1563 Size: 3108 373 3063 Fragments arranged by size: 3108 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4607 5991 6259 1632 Size: 2975 1384 268 1917 Fragments arranged by size: 2975 1917 1384 268 AfeI AGC'GCT Cuts at: 2639 2639 Size: 6544 AflII C'TTAA_G Cuts at: 820 1017 3608 820 Size: 197 2591 3756 Fragments arranged by size: 3756 2591 197 AflIII A'CryG_T Cuts at: 1203 2586 5443 6420 1203 Size: 1383 2857 977 1327 Fragments arranged by size: 2857 1383 1327 977 AgeI A'CCGG_T Cuts at: 5375 5375 Size: 6544 AhdI GACnn_n'nnGTC Cuts at: 5273 5273 Size: 6544 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6544 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3299 1184 Size: 2115 4429 AlwNI CAG_nnn'CTG Cuts at: 2338 3271 3374 4287 5859 2338 Size: 933 103 913 1572 3023 Fragments arranged by size: 3023 1572 933 913 103 ApaI G_GGCC'C Cuts at: 2142 2142 Size: 6544 ApaLI G'TGCA_C Cuts at: 4601 5757 4601 Size: 1156 5388 ApoI r'AATT_y Cuts at: 1493 3853 4079 4231 6340 1493 Size: 2360 226 152 2109 1697 Fragments arranged by size: 2360 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5098 160 Size: 872 4066 1606 Fragments arranged by size: 4066 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2188 2221 2282 3869 1870 Size: 73 127 118 33 61 1587 4545 Fragments arranged by size: 4545 1587 127 118 73 61 33 BamHI G'GATC_C Cuts at: 3686 3874 4241 3686 Size: 188 367 5989 Fragments arranged by size: 5989 367 188 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2039 2152 2411 2863 Size: 325 217 627 233 19 113 259 452 Cuts at: 2863 2941 3122 3865 5320 618 Size: 78 181 743 1455 1842 Fragments arranged by size: 1842 1455 743 627 452 325 259 233 217 181 113 78 19 BanII G_rGCy'C Cuts at: 1391 2106 2130 2142 2741 3497 3863 1391 Size: 715 24 12 599 756 366 4072 Fragments arranged by size: 4072 756 715 599 366 24 12 BbeI G_GCGC'C Cuts at: 2156 2867 2156 Size: 711 5833 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5573 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4735 4769 1903 Size: 34 2798 34 3678 Fragments arranged by size: 3678 2798 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2537 2739 4436 5646 2537 Size: 202 1697 1210 3435 Fragments arranged by size: 3435 1697 1210 202 BclI T'GATC_A Cuts at: 1548 1548 Size: 6544 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2161 2272 2894 Size: 107 122 71 631 605 489 111 622 Cuts at: 2894 5155 136 Size: 2261 1525 Fragments arranged by size: 2261 1525 631 622 605 489 122 111 107 71 BglII A'GATC_T Cuts at: 6539 6539 Size: 6544 BlpI GC'TnA_GC Cuts at: 2129 3961 2129 Size: 1832 4712 Bme1580I G_kGCm'C Cuts at: 1790 2025 2142 2321 2345 2414 3125 3543 3626 Size: 235 117 179 24 69 711 418 83 Cuts at: 3626 3733 4605 5761 1790 Size: 107 872 1156 2573 Fragments arranged by size: 2573 1156 872 711 418 235 179 117 107 83 69 24 BmgBI CAC'GTC Cuts at: 1318 2587 1318 Size: 1269 5275 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4275 5233 442 Size: 416 53 83 399 24 2858 958 1753 Fragments arranged by size: 2858 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6544 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2090 3334 3734 5204 1239 Size: 809 42 1244 400 1470 2579 Fragments arranged by size: 2579 1470 1244 809 400 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4669 5534 5832 4669 Size: 865 298 5381 Fragments arranged by size: 5381 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2807 2981 5207 882 Size: 617 1308 174 2226 2219 Fragments arranged by size: 2226 2219 1308 617 174 BsaAI yAC'GTr Cuts at: 493 1204 2994 4335 493 Size: 711 1790 1341 2702 Fragments arranged by size: 2702 1790 1341 711 BsaBI GATnn'nnATC Cuts at: 6538 6538 Size: 6544 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2153 2348 2864 3411 Size: 53 83 186 936 620 195 516 547 Cuts at: 3411 4351 4733 5368 275 Size: 940 382 635 1451 Fragments arranged by size: 1451 940 936 635 620 547 516 382 195 186 83 53 BsaWI w'CCGG_w Cuts at: 2731 4977 5375 5649 5796 6207 2731 Size: 2246 398 274 147 411 3068 Fragments arranged by size: 3068 2246 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2978 3008 1178 Size: 30 165 30 1575 30 4714 Fragments arranged by size: 4714 1575 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2070 2267 2306 2687 3171 3608 3669 1186 Size: 884 197 39 381 484 437 61 4061 Fragments arranged by size: 4061 884 484 437 381 197 61 39 BseYI C'CCAG_C Cuts at: 2123 2407 2513 2617 3044 3304 3701 5747 6243 Size: 284 106 104 427 260 397 2046 496 Cuts at: 6243 2123 Size: 2424 Fragments arranged by size: 2424 2046 496 427 397 284 260 106 104 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 1163 Size: 573 603 5368 Fragments arranged by size: 5368 603 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2276 2503 3994 4755 5783 1540 Size: 275 186 275 227 1491 761 1028 2301 Fragments arranged by size: 2301 1491 1028 761 275 275 227 186 BsiHKAI G_wGCw'C Cuts at: 2106 2741 4605 4690 5761 2106 Size: 635 1864 85 1071 2889 Fragments arranged by size: 2889 1864 1071 635 85 BsmI GAATG_Cn' Cuts at: 3724 4055 4148 3724 Size: 331 93 6120 Fragments arranged by size: 6120 331 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2172 2393 2581 2773 3556 2172 Size: 221 188 192 783 5160 Fragments arranged by size: 5160 783 221 192 188 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2106 2130 2142 2321 2345 2414 Size: 399 235 81 24 12 179 24 69 Cuts at: 2414 2741 3125 3497 3543 3626 3733 3863 4605 Size: 327 384 372 46 83 107 130 742 Cuts at: 4605 4690 5761 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 384 372 327 235 179 130 107 85 83 81 69 46 24 24 12 BspEI T'CCGG_A Cuts at: 2731 6207 2731 Size: 3476 3068 BspHI T'CATG_A Cuts at: 3072 4433 6163 3072 Size: 1361 1730 3453 Fragments arranged by size: 3453 1730 1361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3899 844 Size: 3055 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2947 4431 1394 Size: 429 1124 1484 3507 Fragments arranged by size: 3507 1484 1124 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3525 5039 5213 65 Size: 1183 2277 1514 174 1396 Fragments arranged by size: 2277 1514 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2149 2508 2566 3598 5188 5375 1700 Size: 110 339 359 58 1032 1590 187 2869 Fragments arranged by size: 2869 1590 1032 359 339 187 110 58 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6544 BssHII G'CGCG_C Cuts at: 1925 2169 1925 Size: 244 6300 BssSI C'ACGA_G Cuts at: 3023 3590 4604 5616 3023 Size: 567 1014 1012 3951 Fragments arranged by size: 3951 1014 1012 567 BstAPI GCAn_nnn'nTGC Cuts at: 794 3728 794 Size: 2934 3610 BstBI TT'CG_AA Cuts at: 5348 5348 Size: 6544 BstEII G'GTnAC_C Cuts at: 1184 3430 1184 Size: 2246 4298 BstXI CCAn_nnnn'nTGG Cuts at: 2962 3190 2962 Size: 228 6316 BstYI r'GATC_y Cuts at: 1972 3267 3686 3874 4241 4626 4643 6095 6181 Size: 1295 419 188 367 385 17 1452 86 Cuts at: 6181 6193 6539 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1295 419 385 367 346 188 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2480 2480 Size: 6544 BtgI C'CryG_G Cuts at: 513 1065 2004 2261 2631 513 Size: 552 939 257 370 4426 Fragments arranged by size: 4426 939 552 370 257 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2076 2148 2743 3493 487 Size: 683 56 850 72 595 750 3538 Fragments arranged by size: 3538 850 750 683 595 72 56 BtsI GCAGTG_nn' Cuts at: 3432 4060 4854 4874 3432 Size: 628 794 20 5102 Fragments arranged by size: 5102 794 628 20 ClaI AT'CG_AT Cuts at: 1554 3084 1554 Size: 1530 5014 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6509 DraI TTT'AAA Cuts at: 3848 3848 Size: 6544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3656 5551 809 Size: 507 2340 1895 1802 Fragments arranged by size: 2340 1895 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2273 2964 Size: 54 1425 50 275 18 253 190 691 Cuts at: 2964 3093 3163 3192 3991 4880 6509 8 Size: 129 70 29 799 889 1629 43 Fragments arranged by size: 1629 1425 889 799 691 275 253 190 129 70 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2273 3991 1537 Size: 275 461 1718 4090 Fragments arranged by size: 4090 1718 461 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2664 3332 3350 3609 4474 1353 Size: 1311 668 18 259 865 3423 Fragments arranged by size: 3423 1311 865 668 259 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2840 3168 3200 5131 5505 5651 Size: 1064 138 1251 328 32 1931 374 146 Cuts at: 5651 387 Size: 1280 Fragments arranged by size: 1931 1280 1251 1064 374 328 146 138 32 EcoICRI GAG'CTC Cuts at: 2104 2739 2104 Size: 635 5909 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2090 3334 3734 4607 5204 5991 Size: 393 416 42 1244 400 873 597 787 Cuts at: 5991 6259 1239 Size: 268 1524 Fragments arranged by size: 1524 1244 873 787 597 416 400 393 268 42 EcoNI CCTnn'n_nnAGG Cuts at: 3295 3295 Size: 6544 EcoO109I rG'GnC_Cy Cuts at: 2446 2958 3517 3708 3798 2446 Size: 512 559 191 90 5192 Fragments arranged by size: 5192 559 512 191 90 EcoRI G'AATT_C Cuts at: 3853 4231 3853 Size: 378 6166 FspI TGC'GCA Cuts at: 5050 5050 Size: 6544 HaeII r_GCGC'y Cuts at: 1678 2156 2461 2641 2707 2867 5691 1678 Size: 478 305 180 66 160 2824 2531 Fragments arranged by size: 2824 2531 478 305 180 160 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 5185 5217 5259 Size: 32 135 32 599 32 2982 32 42 Cuts at: 5259 5291 1373 Size: 32 2626 Fragments arranged by size: 2982 2626 599 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2572 3888 4134 669 Size: 988 281 634 1316 246 3079 Fragments arranged by size: 3079 1316 988 634 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6544 HpaI GTT'AAC Cuts at: 4134 4134 Size: 6544 KasI G'GCGC_C Cuts at: 2152 2863 2152 Size: 711 5833 KpnI G_GTAC'C Cuts at: 3869 3869 Size: 6544 MfeI C'AATT_G Cuts at: 4143 4143 Size: 6544 MluI A'CGCG_T Cuts at: 6420 6420 Size: 6544 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3579 5658 5842 1305 Size: 2274 2079 184 2007 Fragments arranged by size: 2274 2079 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3095 3194 10 Size: 54 1425 343 1263 99 3360 Fragments arranged by size: 3360 1425 1263 343 99 54 MslI CAynn'nnrTG Cuts at: 518 1166 3086 4502 4861 5020 518 Size: 648 1920 1416 359 159 2042 Fragments arranged by size: 2042 1920 1416 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2212 2245 2407 2617 2907 3664 3675 3744 Size: 606 33 162 210 290 757 11 69 Cuts at: 3744 3960 4637 5785 6030 1606 Size: 216 677 1148 245 2120 Fragments arranged by size: 2120 1148 757 677 606 290 245 216 210 162 69 33 11 NaeI GCC'GGC Cuts at: 2151 3600 2151 Size: 1449 5095 NarI GG'CG_CC Cuts at: 2153 2864 2153 Size: 711 5833 NcoI C'CATG_G Cuts at: 513 1065 2004 2631 513 Size: 552 939 627 4426 Fragments arranged by size: 4426 939 627 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6544 NgoMIV G'CCGG_C Cuts at: 2149 3598 2149 Size: 1449 5095 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6544 NotI GC'GGCC_GC Cuts at: 3991 3991 Size: 6544 NspI r_CATG'y Cuts at: 1132 1229 3902 5447 1132 Size: 97 2673 1545 2229 Fragments arranged by size: 2673 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2891 3118 1348 Size: 1543 227 4774 Fragments arranged by size: 4774 1543 227 PciI A'CATG_T Cuts at: 5443 5443 Size: 6544 PflMI CCAn_nnn'nTGG Cuts at: 1108 2338 1108 Size: 1230 5314 PfoI T'CCnGG_A Cuts at: 3062 3062 Size: 6544 PmeI GTTT'AAAC Cuts at: 3848 3848 Size: 6544 PmlI CAC'GTG Cuts at: 2994 2994 Size: 6544 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4613 4645 6126 6158 4613 Size: 32 1481 32 4999 Fragments arranged by size: 4999 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2446 2958 3517 3708 3798 2446 Size: 512 559 191 90 5192 Fragments arranged by size: 5192 559 512 191 90 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6544 PsiI TTA'TAA Cuts at: 4114 4114 Size: 6544 PspOMI G'GGCC_C Cuts at: 2138 2138 Size: 6544 PstI C_TGCA'G Cuts at: 830 3267 3896 830 Size: 2437 629 3478 Fragments arranged by size: 3478 2437 629 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6544 PvuII CAG'CTG Cuts at: 2407 2617 3744 2407 Size: 210 1127 5207 Fragments arranged by size: 5207 1127 210 SacI G_AGCT'C Cuts at: 2106 2741 2106 Size: 635 5909 SalI G'TCGA_C Cuts at: 1936 3886 1936 Size: 1950 4594 SanDI GG'GwC_CC Cuts at: 2446 3708 2446 Size: 1262 5282 SapI GCTCTTCn'nnn_ Cuts at: 3609 3609 Size: 6544 SbfI CC_TGCA'GG Cuts at: 3896 3896 Size: 6544 ScaI AGT'ACT Cuts at: 2492 4792 6504 2492 Size: 2300 1712 2532 Fragments arranged by size: 2532 2300 1712 SfcI C'TryA_G Cuts at: 826 1045 3263 3892 5027 5708 5899 826 Size: 219 2218 629 1135 681 191 1471 Fragments arranged by size: 2218 1471 1135 681 629 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2272 2272 Size: 6544 SfoI GGC'GCC Cuts at: 2154 2865 2154 Size: 711 5833 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6544 SmaI CCC'GGG Cuts at: 1872 2072 2223 3871 1872 Size: 200 151 1648 4545 Fragments arranged by size: 4545 1648 200 151 SmlI C'TyrA_G Cuts at: 820 1017 1943 3608 4648 5549 5811 820 Size: 197 926 1665 1040 901 262 1553 Fragments arranged by size: 1665 1553 1040 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4335 493 Size: 3842 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6544 SphI G_CATG'C Cuts at: 1132 3902 1132 Size: 2770 3774 SrfI GCCC'GGGC Cuts at: 2072 2072 Size: 6544 SspI AAT'ATT Cuts at: 5 52 4468 5 Size: 47 4416 2081 Fragments arranged by size: 4416 2081 47 StuI AGG'CCT Cuts at: 1517 3290 1517 Size: 1773 4771 StyI C'CwwG_G Cuts at: 513 1065 2004 2142 2631 3255 3983 513 Size: 552 939 138 489 624 728 3074 Fragments arranged by size: 3074 939 728 624 552 489 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2761 4571 4588 4741 4926 5371 325 Size: 1255 1181 1810 17 153 185 445 1498 Fragments arranged by size: 1810 1498 1255 1181 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2490 4790 6502 Size: 274 80 33 51 1443 513 2300 1712 Cuts at: 6502 96 Size: 138 Fragments arranged by size: 2300 1712 1443 513 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2978 3010 6504 6536 2978 Size: 32 3494 32 2986 Fragments arranged by size: 3494 2986 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6544 XbaI T'CTAG_A Cuts at: 3880 3880 Size: 6544 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2437 3301 3442 3781 2437 Size: 864 141 339 5200 Fragments arranged by size: 5200 864 339 141 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6544 XmaI C'CCGG_G Cuts at: 1870 2070 2221 3869 1870 Size: 200 151 1648 4545 Fragments arranged by size: 4545 1648 200 151 XmnI GAAnn'nnTTC Cuts at: 4673 4673 Size: 6544 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4352 276 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SwaI