Restriction map for
pFN21AB9882
(Linear) (Six-base) MAPSORT of: KIBB9882.seq Check: 7248 from: 1 to: 1839
KIBB9882.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AccI GT'mk_AC
Cuts at: 0 1355 1839
Size: 1355 484
AfeI AGC'GCT
Cuts at: 0 634 1839
Size: 634 1205
AflII C'TTAA_G
Cuts at: 0 1603 1839
Size: 1603 236
AflIII A'CryG_T
Cuts at: 0 581 1839
Size: 581 1258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1294 1839
Size: 1294 545
AlwNI CAG_nnn'CTG
Cuts at: 0 333 1266 1369 1839
Size: 333 933 103 470
Fragments arranged by size:
933 470 333 103
ApaI G_GGCC'C
Cuts at: 0 137 1839
Size: 137 1702
AvaI C'yCGr_G
Cuts at: 0 65 183 216 277 1839
Size: 65 118 33 61 1562
Fragments arranged by size:
1562 118 65 61 33
BamHI G'GATC_C
Cuts at: 0 1681 1839
Size: 1681 158
BanI G'GyrC_C
Cuts at: 0 15 34 147 406 858 936 1117 1839
Size: 15 19 113 259 452 78 181 722
Fragments arranged by size:
722 452 259 181 113 78 19 15
BanII G_rGCy'C
Cuts at: 0 101 125 137 736 1492 1839
Size: 101 24 12 599 756 347
Fragments arranged by size:
756 599 347 101 24 12
BbeI G_GCGC'C
Cuts at: 0 151 862 1839
Size: 151 711 977
Fragments arranged by size:
977 711 151
BciVI GTATCCnnnnn_n'
Cuts at: 0 532 734 1839
Size: 532 202 1105
Fragments arranged by size:
1105 532 202
BglI GCCn_nnn'nGGC
Cuts at: 0 156 267 889 1839
Size: 156 111 622 950
Fragments arranged by size:
950 622 156 111
BlpI GC'TnA_GC
Cuts at: 0 124 1839
Size: 124 1715
Bme1580I G_kGCm'C
Cuts at: 0 20 137 316 340 409 1120 1538 1621
Size: 20 117 179 24 69 711 418 83
Cuts at: 1621 1728 1839
Size: 107 111
Fragments arranged by size:
711 418 179 117 111 107 83 69
24 20
BmgBI CAC'GTC
Cuts at: 0 582 1839
Size: 582 1257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 85 1329 1729 1839
Size: 43 42 1244 400 110
Fragments arranged by size:
1244 400 110 43 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 802 976 1839
Size: 802 174 863
Fragments arranged by size:
863 802 174
BsaAI yAC'GTr
Cuts at: 0 989 1839
Size: 989 850
BsaHI Gr'CG_yC
Cuts at: 0 148 343 859 1406 1839
Size: 148 195 516 547 433
Fragments arranged by size:
547 516 433 195 148
BsaWI w'CCGG_w
Cuts at: 0 726 1839
Size: 726 1113
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 973 1003 1839
Size: 973 30 836
Fragments arranged by size:
973 836 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 65 262 301 682 1166 1603 1664 1839
Size: 65 197 39 381 484 437 61 175
Fragments arranged by size:
484 437 381 197 175 65 61 39
BseYI C'CCAG_C
Cuts at: 0 118 402 508 612 1039 1299 1696 1839
Size: 118 284 106 104 427 260 397 143
Fragments arranged by size:
427 397 284 260 143 118 106 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1839
Size: 334 1505
BsiEI CG_ry'CG
Cuts at: 0 271 498 1839
Size: 271 227 1341
Fragments arranged by size:
1341 271 227
BsiHKAI G_wGCw'C
Cuts at: 0 101 736 1839
Size: 101 635 1103
Fragments arranged by size:
1103 635 101
BsmI GAATG_Cn'
Cuts at: 0 1719 1839
Size: 1719 120
BsmBI CGTCTCn'nnnn_
Cuts at: 0 167 388 576 768 1551 1839
Size: 167 221 188 192 783 288
Fragments arranged by size:
783 288 221 192 188 167
Bsp1286I G_dGCh'C
Cuts at: 0 20 101 125 137 316 340 409 736
Size: 20 81 24 12 179 24 69 327
Cuts at: 736 1120 1492 1538 1621 1728 1839
Size: 384 372 46 83 107 111
Fragments arranged by size:
384 372 327 179 111 107 83 81
69 46 24 24 20 12
BspEI T'CCGG_A
Cuts at: 0 726 1839
Size: 726 1113
BspHI T'CATG_A
Cuts at: 0 1067 1839
Size: 1067 772
BsrBI CCG'CTC
Cuts at: 0 942 1839
Size: 942 897
BsrDI GCAATG_nn'
Cuts at: 0 1520 1839
Size: 1520 319
BsrFI r'CCGG_y
Cuts at: 0 144 503 561 1593 1839
Size: 144 359 58 1032 246
Fragments arranged by size:
1032 359 246 144 58
BssHII G'CGCG_C
Cuts at: 0 164 1839
Size: 164 1675
BssSI C'ACGA_G
Cuts at: 0 1018 1585 1839
Size: 1018 567 254
Fragments arranged by size:
1018 567 254
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1723 1839
Size: 1723 116
BstEII G'GTnAC_C
Cuts at: 0 1425 1839
Size: 1425 414
BstXI CCAn_nnnn'nTGG
Cuts at: 0 957 1185 1839
Size: 957 228 654
Fragments arranged by size:
957 654 228
BstYI r'GATC_y
Cuts at: 0 1262 1681 1839
Size: 1262 419 158
Fragments arranged by size:
1262 419 158
Bsu36I CC'TnA_GG
Cuts at: 0 475 1839
Size: 475 1364
BtgI C'CryG_G
Cuts at: 0 256 626 1839
Size: 256 370 1213
Fragments arranged by size:
1213 370 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 71 143 738 1488 1839
Size: 71 72 595 750 351
Fragments arranged by size:
750 595 351 72 71
BtsI GCAGTG_nn'
Cuts at: 0 1427 1839
Size: 1427 412
ClaI AT'CG_AT
Cuts at: 0 1079 1839
Size: 1079 760
DrdI GACnn_nn'nnGTC
Cuts at: 0 1651 1839
Size: 1651 188
EaeI y'GGCC_r
Cuts at: 0 78 268 959 1088 1158 1187 1839
Size: 78 190 691 129 70 29 652
Fragments arranged by size:
691 652 190 129 78 70 29
EagI C'GGCC_G
Cuts at: 0 268 1839
Size: 268 1571
EarI CTCTTCn'nnn_
Cuts at: 0 659 1327 1345 1604 1839
Size: 659 668 18 259 235
Fragments arranged by size:
668 659 259 235 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 835 1163 1195 1839
Size: 835 328 32 644
Fragments arranged by size:
835 644 328 32
EcoICRI GAG'CTC
Cuts at: 0 99 734 1839
Size: 99 635 1105
Fragments arranged by size:
1105 635 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 85 1329 1729 1839
Size: 43 42 1244 400 110
Fragments arranged by size:
1244 400 110 43 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1290 1839
Size: 1290 549
EcoO109I rG'GnC_Cy
Cuts at: 0 441 953 1512 1703 1793 1839
Size: 441 512 559 191 90 46
Fragments arranged by size:
559 512 441 191 90 46
HaeII r_GCGC'y
Cuts at: 0 151 456 636 702 862 1839
Size: 151 305 180 66 160 977
Fragments arranged by size:
977 305 180 160 151 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 1839
Size: 166 32 1641
Fragments arranged by size:
1641 166 32
HincII GTy'rAC
Cuts at: 0 567 1839
Size: 567 1272
KasI G'GCGC_C
Cuts at: 0 147 858 1839
Size: 147 711 981
Fragments arranged by size:
981 711 147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1574 1839
Size: 1574 265
MscI TGG'CCA
Cuts at: 0 1090 1189 1839
Size: 1090 99 650
Fragments arranged by size:
1090 650 99
MslI CAynn'nnrTG
Cuts at: 0 1081 1839
Size: 1081 758
MspA1I CmG'CkG
Cuts at: 0 207 240 402 612 902 1659 1670 1739
Size: 207 33 162 210 290 757 11 69
Cuts at: 1739 1839
Size: 100
Fragments arranged by size:
757 290 210 207 162 100 69 33
11
NaeI GCC'GGC
Cuts at: 0 146 1595 1839
Size: 146 1449 244
Fragments arranged by size:
1449 244 146
NarI GG'CG_CC
Cuts at: 0 148 859 1839
Size: 148 711 980
Fragments arranged by size:
980 711 148
NcoI C'CATG_G
Cuts at: 0 626 1839
Size: 626 1213
NgoMIV G'CCGG_C
Cuts at: 0 144 1593 1839
Size: 144 1449 246
Fragments arranged by size:
1449 246 144
PasI CC'CwG_GG
Cuts at: 0 886 1113 1839
Size: 886 227 726
Fragments arranged by size:
886 726 227
PflMI CCAn_nnn'nTGG
Cuts at: 0 333 1839
Size: 333 1506
PfoI T'CCnGG_A
Cuts at: 0 1057 1839
Size: 1057 782
PmlI CAC'GTG
Cuts at: 0 989 1839
Size: 989 850
PpuMI rG'GwC_Cy
Cuts at: 0 441 953 1512 1703 1793 1839
Size: 441 512 559 191 90 46
Fragments arranged by size:
559 512 441 191 90 46
PspOMI G'GGCC_C
Cuts at: 0 133 1839
Size: 133 1706
PstI C_TGCA'G
Cuts at: 0 1262 1839
Size: 1262 577
PvuII CAG'CTG
Cuts at: 0 402 612 1739 1839
Size: 402 210 1127 100
Fragments arranged by size:
1127 402 210 100
SacI G_AGCT'C
Cuts at: 0 101 736 1839
Size: 101 635 1103
Fragments arranged by size:
1103 635 101
SanDI GG'GwC_CC
Cuts at: 0 441 1703 1839
Size: 441 1262 136
Fragments arranged by size:
1262 441 136
SapI GCTCTTCn'nnn_
Cuts at: 0 1604 1839
Size: 1604 235
ScaI AGT'ACT
Cuts at: 0 487 1839
Size: 487 1352
SfcI C'TryA_G
Cuts at: 0 1258 1839
Size: 1258 581
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 267 1839
Size: 267 1572
SfoI GGC'GCC
Cuts at: 0 149 860 1839
Size: 149 711 979
Fragments arranged by size:
979 711 149
SmaI CCC'GGG
Cuts at: 0 67 218 1839
Size: 67 151 1621
Fragments arranged by size:
1621 151 67
SmlI C'TyrA_G
Cuts at: 0 1603 1839
Size: 1603 236
SrfI GCCC'GGGC
Cuts at: 0 67 1839
Size: 67 1772
StuI AGG'CCT
Cuts at: 0 1285 1839
Size: 1285 554
StyI C'CwwG_G
Cuts at: 0 137 626 1250 1839
Size: 137 489 624 589
Fragments arranged by size:
624 589 489 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 756 1839
Size: 756 1083
TatI w'GTAC_w
Cuts at: 0 485 1839
Size: 485 1354
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 973 1005 1839
Size: 973 32 834
Fragments arranged by size:
973 834 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 432 1296 1437 1776 1839
Size: 432 864 141 339 63
Fragments arranged by size:
864 432 339 141 63
XmaI C'CCGG_G
Cuts at: 0 65 216 1839
Size: 65 151 1623
Fragments arranged by size:
1623 151 65
Enzymes that do cut:
AccI AfeI AflII AflIII AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BciVI BglI BlpI Bme1580I
BmgBI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV PasI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SanDI SapI ScaI
SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BclI BfrBI BglII BmrI BmtI
BplI Bpu10I BpuEI BsaBI BsiWI BspMI BsrGI BstBI
BstZ17I CspCI DraI DraIII EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI SexAI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XhoI XmnI ZraI