(Circular) (Six-base) MAPSORT of: pFN21AB9868.seq Check: 6357 from: 1 to: 6055 pFN21AB9868 6055 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2086 2086 Size: 6055 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2649 3398 1937 Size: 712 749 4594 Fragments arranged by size: 4594 749 712 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2453 4118 5502 5770 1632 Size: 821 1665 1384 268 1917 Fragments arranged by size: 1917 1665 1384 821 268 AfeI AGC'GCT Cuts at: 3116 3116 Size: 6055 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 4954 5931 1203 Size: 3751 977 1327 Fragments arranged by size: 3751 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 4784 4784 Size: 6055 AleI CACnn'nnGTG Cuts at: 1166 2687 3125 1166 Size: 1521 438 4096 Fragments arranged by size: 4096 1521 438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2705 1184 Size: 1521 4534 AlwNI CAG_nnn'CTG Cuts at: 3798 5370 3798 Size: 1572 4483 ApaI G_GGCC'C Cuts at: 2768 2786 2928 2768 Size: 18 142 5895 Fragments arranged by size: 5895 142 18 ApaLI G'TGCA_C Cuts at: 2369 4112 5268 2369 Size: 1743 1156 3156 Fragments arranged by size: 3156 1743 1156 ApoI r'AATT_y Cuts at: 1493 3230 3364 3590 3742 5851 1493 Size: 1737 134 226 152 2109 1697 Fragments arranged by size: 2109 1737 1697 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2768 2785 2936 2949 2967 3380 Size: 73 130 695 17 151 13 18 413 Cuts at: 3380 1870 Size: 4545 Fragments arranged by size: 4545 695 413 151 130 73 18 17 13 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2069 2200 2738 2828 Size: 325 217 627 269 13 131 538 90 Cuts at: 2828 3376 4831 618 Size: 548 1455 1842 Fragments arranged by size: 1842 1455 627 548 538 325 269 217 131 90 13 BanII G_rGCy'C Cuts at: 1391 2012 2768 2786 2844 2894 2928 3374 1391 Size: 621 756 18 58 50 34 446 4072 Fragments arranged by size: 4072 756 621 446 58 50 34 18 BbeI G_GCGC'C Cuts at: 2060 2073 2204 2832 2060 Size: 13 131 628 5283 Fragments arranged by size: 5283 628 131 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2889 928 Size: 971 990 4094 Fragments arranged by size: 4094 990 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2143 2177 4246 4280 1903 Size: 34 206 34 2069 34 3678 Fragments arranged by size: 3678 2069 206 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 1548 Size: 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2124 2790 2854 Size: 107 122 71 631 605 452 666 64 Cuts at: 2854 2923 4666 136 Size: 69 1743 1525 Fragments arranged by size: 1743 1525 666 631 605 452 122 107 71 69 64 BglII A'GATC_T Cuts at: 2325 6050 2325 Size: 3725 2330 BlpI GC'TnA_GC Cuts at: 3472 3472 Size: 6055 Bme1580I G_kGCm'C Cuts at: 1790 2373 2741 2768 2786 2928 4116 5272 1790 Size: 583 368 27 18 142 1188 1156 2573 Fragments arranged by size: 2573 1188 1156 583 368 142 27 18 BmgBI CAC'GTC Cuts at: 1318 2344 2358 1318 Size: 1026 14 5015 Fragments arranged by size: 5015 1026 14 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3786 4744 442 Size: 416 53 83 399 24 2369 958 1753 Fragments arranged by size: 2369 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2908 2908 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2903 2904 4715 1239 Size: 1664 1 1811 2579 Fragments arranged by size: 2579 1811 1664 1 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2180 4180 5045 5343 2180 Size: 2000 865 298 2892 Fragments arranged by size: 2892 2000 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4718 882 Size: 617 3219 2219 Fragments arranged by size: 3219 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2653 3846 493 Size: 711 1449 1193 2702 Fragments arranged by size: 2702 1449 1193 711 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 2070 2201 2715 Size: 53 83 186 936 524 13 131 514 Cuts at: 2715 2829 3035 3080 3862 4244 4879 275 Size: 114 206 45 782 382 635 1451 Fragments arranged by size: 1451 936 782 635 524 514 382 206 186 131 114 83 53 45 13 BsaWI w'CCGG_w Cuts at: 2710 4488 4886 5160 5307 5718 2710 Size: 1778 398 274 147 411 3047 Fragments arranged by size: 3047 1778 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2879 2909 3138 3168 1178 Size: 30 165 30 1476 30 229 30 4065 Fragments arranged by size: 4065 1476 229 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2900 3071 1186 Size: 1714 171 4170 Fragments arranged by size: 4170 1714 171 BseYI C'CCAG_C Cuts at: 2696 2817 5258 5754 2696 Size: 121 2441 496 2997 Fragments arranged by size: 2997 2441 496 121 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2338 1163 Size: 573 602 4880 Fragments arranged by size: 4880 602 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2238 2490 2556 2853 3198 3505 Size: 275 186 237 252 66 297 345 307 Cuts at: 3505 4266 5294 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 345 307 297 275 252 237 186 66 BsiHKAI G_wGCw'C Cuts at: 2163 2349 2373 2894 3209 4116 4201 5272 2163 Size: 186 24 521 315 907 85 1071 2946 Fragments arranged by size: 2946 1071 907 521 315 186 85 24 BsmI GAATG_Cn' Cuts at: 3566 3659 3566 Size: 93 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2163 2349 2373 2741 2768 2786 Size: 399 222 151 186 24 368 27 18 Cuts at: 2786 2844 2894 2928 3209 3374 4116 4201 5272 Size: 58 50 34 281 165 742 85 1071 Cuts at: 5272 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 368 281 222 186 165 151 85 58 50 34 27 24 18 BspEI T'CCGG_A Cuts at: 2710 5718 2710 Size: 3008 3047 BspHI T'CATG_A Cuts at: 2637 3944 5674 2637 Size: 1307 1730 3018 Fragments arranged by size: 3018 1730 1307 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2086 3410 844 Size: 1242 1324 3489 Fragments arranged by size: 3489 1324 1242 BsrBI CCG'CTC Cuts at: 1394 1823 2509 2914 3942 1394 Size: 429 686 405 1028 3507 Fragments arranged by size: 3507 1028 686 429 405 BsrDI GCAATG_nn' Cuts at: 65 1248 4550 4724 65 Size: 1183 3302 174 1396 Fragments arranged by size: 3302 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2123 2551 2848 3037 4699 4886 1700 Size: 110 313 428 297 189 1662 187 2869 Fragments arranged by size: 2869 1662 428 313 297 189 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 2190 1925 Size: 265 5790 BssSI C'ACGA_G Cuts at: 2182 4115 5127 2182 Size: 1933 1012 3110 Fragments arranged by size: 3110 1933 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6055 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6055 BstXI CCAn_nnnn'nTGG Cuts at: 2389 2761 2389 Size: 372 5683 BstYI r'GATC_y Cuts at: 1972 2325 3321 3385 3752 4137 4154 5606 5692 Size: 353 996 64 367 385 17 1452 86 Cuts at: 5692 5704 6050 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 996 385 367 353 346 86 64 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3002 3135 513 Size: 552 939 998 133 3433 Fragments arranged by size: 3433 998 939 552 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2155 487 Size: 683 56 929 4387 Fragments arranged by size: 4387 929 683 56 BtsI GCAGTG_nn' Cuts at: 3571 4365 4385 3571 Size: 794 20 5241 Fragments arranged by size: 5241 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DraIII CAC_nnn'GTG Cuts at: 2475 2475 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2235 2250 Size: 54 1425 50 275 18 291 114 15 Cuts at: 2250 2277 2487 2553 2850 3132 3195 3502 4391 Size: 27 210 66 297 282 63 307 889 Cuts at: 4391 6020 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 307 297 291 282 275 210 114 66 63 54 50 43 27 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2235 2487 2553 2850 3195 3502 1537 Size: 275 423 252 66 297 345 307 4090 Fragments arranged by size: 4090 423 345 307 297 275 252 66 EarI CTCTTCn'nnn_ Cuts at: 1353 2219 2354 3230 3985 1353 Size: 866 135 876 755 3423 Fragments arranged by size: 3423 876 866 755 135 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3293 4642 5016 5162 387 Size: 1064 138 1704 1349 374 146 1280 Fragments arranged by size: 1704 1349 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2892 2892 Size: 6055 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 2903 2904 4118 4715 5502 5770 Size: 393 821 450 1 1214 597 787 268 Cuts at: 5770 1239 Size: 1524 Fragments arranged by size: 1524 1214 821 787 597 450 393 268 1 EcoO109I rG'GnC_Cy Cuts at: 2765 2924 2925 2765 Size: 159 1 5895 Fragments arranged by size: 5895 159 1 EcoRI G'AATT_C Cuts at: 3364 3742 3364 Size: 378 5677 FseI GG_CCGG'CC Cuts at: 2127 2127 Size: 6055 FspI TGC'GCA Cuts at: 2657 4561 2657 Size: 1904 4151 FspAI rTGC'GCAy Cuts at: 2657 2657 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 2060 2073 2204 2339 2832 3118 5202 1678 Size: 382 13 131 135 493 286 2084 2531 Fragments arranged by size: 2531 2084 493 382 286 135 131 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2879 2911 3313 3345 4696 Size: 32 135 32 1307 32 402 32 1351 Cuts at: 4696 4728 4770 4802 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1351 1307 402 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3399 3645 669 Size: 988 281 1461 246 3079 Fragments arranged by size: 3079 1461 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KasI G'GCGC_C Cuts at: 2056 2069 2200 2828 2056 Size: 13 131 628 5283 Fragments arranged by size: 5283 628 131 13 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2925 5169 5353 1305 Size: 1620 2244 184 2007 Fragments arranged by size: 2244 2007 1620 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 2279 10 Size: 54 1425 343 420 27 3786 Fragments arranged by size: 3786 1425 420 343 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2174 2246 2471 2687 3125 3146 4013 Size: 648 1008 72 225 216 438 21 867 Cuts at: 4013 4372 4531 518 Size: 359 159 2042 Fragments arranged by size: 2042 1008 867 648 438 359 225 216 159 72 21 MspA1I CmG'CkG Cuts at: 1606 2809 3004 3471 4148 5296 5541 1606 Size: 1203 195 467 677 1148 245 2120 Fragments arranged by size: 2120 1203 1148 677 467 245 195 NaeI GCC'GGC Cuts at: 2125 2553 2850 3039 2125 Size: 428 297 189 5141 Fragments arranged by size: 5141 428 297 189 NarI GG'CG_CC Cuts at: 2057 2070 2201 2829 2057 Size: 13 131 628 5283 Fragments arranged by size: 5283 628 131 13 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4564 Fragments arranged by size: 4564 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NgoMIV G'CCGG_C Cuts at: 2123 2551 2848 3037 2123 Size: 428 297 189 5141 Fragments arranged by size: 5141 428 297 189 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 3413 4958 1132 Size: 97 2184 1545 2229 Fragments arranged by size: 2229 2184 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6055 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6055 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PspOMI G'GGCC_C Cuts at: 2764 2782 2924 2764 Size: 18 142 5895 Fragments arranged by size: 5895 142 18 PstI C_TGCA'G Cuts at: 830 2019 2097 3046 3407 830 Size: 1189 78 949 361 3478 Fragments arranged by size: 3478 1189 949 361 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 SacI G_AGCT'C Cuts at: 2894 2894 Size: 6055 SacII CC_GC'GG Cuts at: 3005 3005 Size: 6055 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SapI GCTCTTCn'nnn_ Cuts at: 2354 3230 2354 Size: 876 5179 SbfI CC_TGCA'GG Cuts at: 2097 3407 2097 Size: 1310 4745 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SfcI C'TryA_G Cuts at: 826 1045 2015 2093 3042 3403 4538 5219 5410 Size: 219 970 78 949 361 1135 681 191 Cuts at: 5410 826 Size: 1471 Fragments arranged by size: 1471 1135 970 949 681 361 219 191 78 SfiI GGCCn_nnn'nGGCC Cuts at: 2923 2923 Size: 6055 SfoI GGC'GCC Cuts at: 2058 2071 2202 2830 2058 Size: 13 131 628 5283 Fragments arranged by size: 5283 628 131 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 2075 2787 2951 3382 1872 Size: 203 712 164 431 4545 Fragments arranged by size: 4545 712 431 203 164 SmlI C'TyrA_G Cuts at: 820 1017 1943 2195 4159 5060 5322 820 Size: 197 926 252 1964 901 262 1553 Fragments arranged by size: 1964 1553 926 901 262 252 197 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 3413 1132 Size: 2281 3774 SrfI GCCC'GGGC Cuts at: 2075 2075 Size: 6055 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6055 StyI C'CwwG_G Cuts at: 513 1065 2004 3075 3494 513 Size: 552 939 1071 419 3074 Fragments arranged by size: 3074 1071 939 552 419 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2066 2710 2831 4082 4099 4252 4437 Size: 1255 486 644 121 1251 17 153 185 Cuts at: 4437 4882 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1251 644 486 445 185 153 121 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2424 4301 6013 Size: 274 80 33 51 1443 447 1877 1712 Cuts at: 6013 96 Size: 138 Fragments arranged by size: 1877 1712 1443 447 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3138 3170 6015 6047 3138 Size: 32 2845 32 3146 Fragments arranged by size: 3146 2845 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2717 1316 Size: 1401 4654 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2287 2287 Size: 6055 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 2073 2785 2949 3380 1870 Size: 203 712 164 431 4545 Fragments arranged by size: 4545 712 431 203 164 XmnI GAAnn'nnTTC Cuts at: 3116 4184 3116 Size: 1068 4987 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SexAI SwaI