Restriction map for pFN21AB9868
 (Linear) (Six-base) MAPSORT of: KIBB9868.seq  Check: 6412  from: 1  to: 1350



KIBB9868.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      81    1350 
   Size:         81    1269
 
AccI GT'mk_AC

Cuts at:      0     644    1350 
   Size:        644     706
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448    1350 
   Size:        448     902
 
AfeI AGC'GCT

Cuts at:      0    1111    1350 
   Size:       1111     239
 
AleI CACnn'nnGTG

Cuts at:      0     682    1120    1350 
   Size:        682     438     230

  Fragments arranged by size: 

                 682     438     230
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     700    1350 
   Size:        700     650
 
ApaI G_GGCC'C

Cuts at:      0     763     781     923    1350 
   Size:        763      18     142     427

  Fragments arranged by size: 

                 763     427     142      18
 
ApaLI G'TGCA_C

Cuts at:      0     364    1350 
   Size:        364     986
 
ApoI r'AATT_y

Cuts at:      0    1225    1350 
   Size:       1225     125
 
AvaI C'yCGr_G

Cuts at:      0      68     763     780     931     944     962    1350 
   Size:         68     695      17     151      13      18     388

  Fragments arranged by size: 

                 695     388     151      68      18      17      13
 
BanI G'GyrC_C

Cuts at:      0      51      64     195     733     823    1350 
   Size:         51      13     131     538      90     527

  Fragments arranged by size: 

                 538     527     131      90      51      13
 
BanII G_rGCy'C

Cuts at:      0       7     763     781     839     889     923    1350 
   Size:          7     756      18      58      50      34     427

  Fragments arranged by size: 

                 756     427      58      50      34      18       7
 
BbeI G_GCGC'C

Cuts at:      0      55      68     199     827    1350 
   Size:         55      13     131     628     523

  Fragments arranged by size: 

                 628     523     131      55      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     884    1350 
   Size:        884     466
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     138     172    1350 
   Size:        138      34    1178

  Fragments arranged by size: 

                1178     138      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     119     785     849     918    1350 
   Size:        119     666      64      69     432

  Fragments arranged by size: 

                 666     432     119      69      64
 
BglII A'GATC_T

Cuts at:      0     320    1350 
   Size:        320    1030
 
Bme1580I G_kGCm'C

Cuts at:      0     368     736     763     781     923    1350 
   Size:        368     368      27      18     142     427

  Fragments arranged by size: 

                 427     368     368     142      27      18
 
BmgBI CAC'GTC

Cuts at:      0     339     353    1350 
   Size:        339      14     997

  Fragments arranged by size: 

                 997     339      14
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     903    1350 
   Size:        903     447
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     898     899    1350 
   Size:        898       1     451

  Fragments arranged by size: 

                 898     451       1
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175    1350 
   Size:        175    1175
 
BsaAI yAC'GTr

Cuts at:      0     648    1350 
   Size:        648     702
 
BsaHI Gr'CG_yC

Cuts at:      0      52      65     196     710     824    1030    1075    1350 
   Size:         52      13     131     514     114     206      45     275

  Fragments arranged by size: 

                 514     275     206     131     114      52      45      13
            
 
BsaWI w'CCGG_w

Cuts at:      0     705    1350 
   Size:        705     645
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     874     904    1133    1163    1350 
   Size:        874      30     229      30     187

  Fragments arranged by size: 

                 874     229     187      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     895    1066    1350 
   Size:        895     171     284

  Fragments arranged by size: 

                 895     284     171
 
BseYI C'CCAG_C

Cuts at:      0     691     812    1350 
   Size:        691     121     538

  Fragments arranged by size: 

                 691     538     121
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333    1350 
   Size:        333    1017
 
BsiEI CG_ry'CG

Cuts at:      0     233     485     551     848    1193    1350 
   Size:        233     252      66     297     345     157

  Fragments arranged by size: 

                 345     297     252     233     157      66
 
BsiHKAI G_wGCw'C

Cuts at:      0     158     344     368     889    1204    1350 
   Size:        158     186      24     521     315     146

  Fragments arranged by size: 

                 521     315     186     158     146      24
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     158     344     368     736     763     781     839 
   Size:          7     151     186      24     368      27      18      58

Cuts at:    839     889     923    1204    1350 
   Size:         50      34     281     146

  Fragments arranged by size: 

                 368     281     186     151     146      58      50      34
                  27      24      18       7
 
BspEI T'CCGG_A

Cuts at:      0     705    1350 
   Size:        705     645
 
BspHI T'CATG_A

Cuts at:      0     632    1350 
   Size:        632     718
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      81    1350 
   Size:         81    1269
 
BsrBI CCG'CTC

Cuts at:      0     504     909    1350 
   Size:        504     405     441

  Fragments arranged by size: 

                 504     441     405
 
BsrFI r'CCGG_y

Cuts at:      0     118     546     843    1032    1350 
   Size:        118     428     297     189     318

  Fragments arranged by size: 

                 428     318     297     189     118
 
BssHII G'CGCG_C

Cuts at:      0     185    1350 
   Size:        185    1165
 
BssSI C'ACGA_G

Cuts at:      0     177    1350 
   Size:        177    1173
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     384     756    1350 
   Size:        384     372     594

  Fragments arranged by size: 

                 594     384     372
 
BstYI r'GATC_y

Cuts at:      0     320    1316    1350 
   Size:        320     996      34

  Fragments arranged by size: 

                 996     320      34
 
BtgI C'CryG_G

Cuts at:      0     997    1130    1350 
   Size:        997     133     220

  Fragments arranged by size: 

                 997     220     133
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     150    1350 
   Size:        150    1200
 
DraIII CAC_nnn'GTG

Cuts at:      0     470    1350 
   Size:        470     880
 
EaeI y'GGCC_r

Cuts at:      0     116     230     245     272     482     548     845    1127 
   Size:        116     114      15      27     210      66     297     282

Cuts at:   1127    1190    1350 
   Size:         63     160

  Fragments arranged by size: 

                 297     282     210     160     116     114      66      63
                  27      15
 
EagI C'GGCC_G

Cuts at:      0     230     482     548     845    1190    1350 
   Size:        230     252      66     297     345     160

  Fragments arranged by size: 

                 345     297     252     230     160      66
 
EarI CTCTTCn'nnn_

Cuts at:      0     214     349    1225    1350 
   Size:        214     135     876     125

  Fragments arranged by size: 

                 876     214     135     125
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1288    1350 
   Size:       1288      62
 
EcoICRI GAG'CTC

Cuts at:      0     887    1350 
   Size:        887     463
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     898     899    1350 
   Size:        448     450       1     451

  Fragments arranged by size: 

                 451     450     448       1
 
EcoO109I rG'GnC_Cy

Cuts at:      0     760     919     920    1350 
   Size:        760     159       1     430

  Fragments arranged by size: 

                 760     430     159       1
 
FseI GG_CCGG'CC

Cuts at:      0     122    1350 
   Size:        122    1228
 
FspI TGC'GCA

Cuts at:      0     652    1350 
   Size:        652     698
 
FspAI rTGC'GCAy

Cuts at:      0     652    1350 
   Size:        652     698
 
HaeII r_GCGC'y

Cuts at:      0      55      68     199     334     827    1113    1350 
   Size:         55      13     131     135     493     286     237

  Fragments arranged by size: 

                 493     286     237     135     131      55      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     874     906    1308    1340    1350 
   Size:        874      32     402      32      10

  Fragments arranged by size: 

                 874     402      32      32      10
 
KasI G'GCGC_C

Cuts at:      0      51      64     195     823    1350 
   Size:         51      13     131     628     527

  Fragments arranged by size: 

                 628     527     131      51      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     920    1350 
   Size:        920     430
 
MscI TGG'CCA

Cuts at:      0     247     274    1350 
   Size:        247      27    1076

  Fragments arranged by size: 

                1076     247      27
 
MslI CAynn'nnrTG

Cuts at:      0     169     241     466     682    1120    1141    1350 
   Size:        169      72     225     216     438      21     209

  Fragments arranged by size: 

                 438     225     216     209     169      72      21
 
MspA1I CmG'CkG

Cuts at:      0     804     999    1350 
   Size:        804     195     351

  Fragments arranged by size: 

                 804     351     195
 
NaeI GCC'GGC

Cuts at:      0     120     548     845    1034    1350 
   Size:        120     428     297     189     316

  Fragments arranged by size: 

                 428     316     297     189     120
 
NarI GG'CG_CC

Cuts at:      0      52      65     196     824    1350 
   Size:         52      13     131     628     526

  Fragments arranged by size: 

                 628     526     131      52      13
 
NgoMIV G'CCGG_C

Cuts at:      0     118     546     843    1032    1350 
   Size:        118     428     297     189     318

  Fragments arranged by size: 

                 428     318     297     189     118
 
PspOMI G'GGCC_C

Cuts at:      0     759     777     919    1350 
   Size:        759      18     142     431

  Fragments arranged by size: 

                 759     431     142      18
 
PstI C_TGCA'G

Cuts at:      0      14      92    1041    1350 
   Size:         14      78     949     309

  Fragments arranged by size: 

                 949     309      78      14
 
SacI G_AGCT'C

Cuts at:      0     889    1350 
   Size:        889     461
 
SacII CC_GC'GG

Cuts at:      0    1000    1350 
   Size:       1000     350
 
SapI GCTCTTCn'nnn_

Cuts at:      0     349    1225    1350 
   Size:        349     876     125

  Fragments arranged by size: 

                 876     349     125
 
SbfI CC_TGCA'GG

Cuts at:      0      92    1350 
   Size:         92    1258
 
SfcI C'TryA_G

Cuts at:      0      10      88    1037    1350 
   Size:         10      78     949     313

  Fragments arranged by size: 

                 949     313      78      10
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     918    1350 
   Size:        918     432
 
SfoI GGC'GCC

Cuts at:      0      53      66     197     825    1350 
   Size:         53      13     131     628     525

  Fragments arranged by size: 

                 628     525     131      53      13
 
SmaI CCC'GGG

Cuts at:      0      70     782     946    1350 
   Size:         70     712     164     404

  Fragments arranged by size: 

                 712     404     164      70
 
SmlI C'TyrA_G

Cuts at:      0     190    1350 
   Size:        190    1160
 
SrfI GCCC'GGGC

Cuts at:      0      70    1350 
   Size:         70    1280
 
StyI C'CwwG_G

Cuts at:      0    1070    1350 
   Size:       1070     280
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      61     705     826    1350 
   Size:         61     644     121     524

  Fragments arranged by size: 

                 644     524     121      61
 
TatI w'GTAC_w

Cuts at:      0     419    1350 
   Size:        419     931
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1133    1165    1350 
   Size:       1133      32     185

  Fragments arranged by size: 

                1133     185      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     712    1350 
   Size:        712     638
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     282    1350 
   Size:        282    1068
 
XmaI C'CCGG_G

Cuts at:      0      68     780     944    1350 
   Size:         68     712     164     406

  Fragments arranged by size: 

                 712     406     164      68
 
XmnI GAAnn'nnTTC

Cuts at:      0    1111    1350 
   Size:       1111     239
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AleI     AlfI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI     BcgI     BglI
    BglII Bme1580I    BmgBI     BplI     BpmI    BpuEI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstXI
    BstYI     BtgI    BtgZI   DraIII     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     FseI     FspI    FspAI    HaeII    Hin4I
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
   PspOMI     PstI     SacI    SacII     SapI     SbfI     SfcI     SfiI
     SfoI     SmaI     SmlI     SrfI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AloI
    AlwNI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
    BciVI     BclI    BfrBI     BlpI     BmrI     BmtI   Bpu10I     BsaI
    BsaBI    BsiWI     BsmI    BsmBI    BsrDI    BsrGI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI     DrdI
    EcoNI    EcoRI    EcoRV     FalI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SalI    SanDI     ScaI    SexAI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SspI     StuI     SwaI     XbaI     XhoI     ZraI
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