Restriction map for pFN21AB9858
 (Linear) (Six-base) MAPSORT of: KIBB9858.seq  Check: 9108  from: 1  to: 1200



KIBB9858.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1102    1200 
   Size:       1102      98
 
AhdI GACnn_n'nnGTC

Cuts at:      0     664    1200 
   Size:        664     536
 
AlwNI CAG_nnn'CTG

Cuts at:      0     775    1165    1200 
   Size:        775     390      35

  Fragments arranged by size: 

                 775     390      35
 
ApaI G_GGCC'C

Cuts at:      0     861    1200 
   Size:        861     339
 
BamHI G'GATC_C

Cuts at:      0     220     376    1200 
   Size:        220     156     824

  Fragments arranged by size: 

                 824     220     156
 
BanI G'GyrC_C

Cuts at:      0     244     487     541     830    1200 
   Size:        244     243      54     289     370

  Fragments arranged by size: 

                 370     289     244     243      54
 
BanII G_rGCy'C

Cuts at:      0     116     124     447     641     861     982    1063    1175 
   Size:        116       8     323     194     220     121      81     112

Cuts at:   1175    1200 
   Size:         25

  Fragments arranged by size: 

                 323     220     194     121     116     112      81      25
                   8
 
BbeI G_GCGC'C

Cuts at:      0     834    1200 
   Size:        834     366
 
BbvCI CC'TCA_GC

Cuts at:      0     186    1200 
   Size:        186    1014
 
BglI GCCn_nnn'nGGC

Cuts at:      0     793    1200 
   Size:        793     407
 
BlpI GC'TnA_GC

Cuts at:      0     868    1200 
   Size:        868     332
 
Bme1580I G_kGCm'C

Cuts at:      0     317     731     861    1200 
   Size:        317     414     130     339

  Fragments arranged by size: 

                 414     339     317     130
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     182     602     863    1200 
   Size:        182     420     261     337

  Fragments arranged by size: 

                 420     337     261     182
 
BmtI G_CTAG'C

Cuts at:      0     467    1200 
   Size:        467     733
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     411     465     493     547     601     627     655 
   Size:        401      10      54      28      54      54      26      28

Cuts at:    655    1200 
   Size:        545

  Fragments arranged by size: 

                 545     401      54      54      54      28      28      26
                  10
 
Bpu10I CC'TnA_GC

Cuts at:      0     118     186     269    1200 
   Size:        118      68      83     931

  Fragments arranged by size: 

                 931     118      83      68
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     105     469     577     672    1200 
   Size:        105     364     108      95     528

  Fragments arranged by size: 

                 528     364     108     105      95
 
BsaHI Gr'CG_yC

Cuts at:      0     831     902    1154    1200 
   Size:        831      71     252      46

  Fragments arranged by size: 

                 831     252      71      46
 
BsaWI w'CCGG_w

Cuts at:      0      85     734    1200 
   Size:         85     649     466

  Fragments arranged by size: 

                 649     466      85
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     399     429     434     464    1069    1099    1200 
   Size:        399      30       5      30     605      30     101

  Fragments arranged by size: 

                 605     399     101      30      30      30       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     946    1090    1129    1132    1200 
   Size:        946     144      39       3      68

  Fragments arranged by size: 

                 946     144      68      39       3
 
BseYI C'CCAG_C

Cuts at:      0     910    1099    1158    1200 
   Size:        910     189      59      42

  Fragments arranged by size: 

                 910     189      59      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91    1200 
   Size:         91    1109
 
BsiHKAI G_wGCw'C

Cuts at:      0      61     124    1200 
   Size:         61      63    1076

  Fragments arranged by size: 

                1076      63      61
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1178    1200 
   Size:       1178      22
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     116     124     317     447     641     731     861 
   Size:         61      55       8     193     130     194      90     130

Cuts at:    861     982    1063    1175    1200 
   Size:        121      81     112      25

  Fragments arranged by size: 

                 194     193     130     130     121     112      90      81
                  61      55      25       8
 
BspEI T'CCGG_A

Cuts at:      0      85     734    1200 
   Size:         85     649     466

  Fragments arranged by size: 

                 649     466      85
 
BsrFI r'CCGG_y

Cuts at:      0     833    1200 
   Size:        833     367
 
BsrGI T'GTAC_A

Cuts at:      0     131    1200 
   Size:        131    1069
 
BssSI C'ACGA_G

Cuts at:      0     755     808     862    1200 
   Size:        755      53      54     338

  Fragments arranged by size: 

                 755     338      54      53
 
BstYI r'GATC_y

Cuts at:      0     220     376    1200 
   Size:        220     156     824

  Fragments arranged by size: 

                 824     220     156
 
Bsu36I CC'TnA_GG

Cuts at:      0     158     929    1200 
   Size:        158     771     271

  Fragments arranged by size: 

                 771     271     158
 
BtgI C'CryG_G

Cuts at:      0     455     509     563     953    1200 
   Size:        455      54      54     390     247

  Fragments arranged by size: 

                 455     390     247      54      54
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1084    1200 
   Size:       1084     116
 
BtsI GCAGTG_nn'

Cuts at:      0      74    1200 
   Size:         74    1126
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1017    1052    1200 
   Size:       1017      35     148

  Fragments arranged by size: 

                1017     148      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     387    1200 
   Size:        387     813
 
EaeI y'GGCC_r

Cuts at:      0       2    1130    1200 
   Size:          2    1128      70

  Fragments arranged by size: 

                1128      70       2
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     864    1200 
   Size:        864     336
 
EcoICRI GAG'CTC

Cuts at:      0     122    1200 
   Size:        122    1078
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     411     465     493     547     601     627     655 
   Size:        401      10      54      28      54      54      26      28

Cuts at:    655    1102    1200 
   Size:        447      98

  Fragments arranged by size: 

                 447     401      98      54      54      54      28      28
                  26      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     429    1200 
   Size:        429     771
 
EcoO109I rG'GnC_Cy

Cuts at:      0     857     858     924    1096    1200 
   Size:        857       1      66     172     104

  Fragments arranged by size: 

                 857     172     104      66       1
 
HaeII r_GCGC'y

Cuts at:      0     834    1200 
   Size:        834     366
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     169     201     650     682     736     768     964     996 
   Size:        169      32     449      32      54      32     196      32

Cuts at:    996    1069    1101    1200 
   Size:         73      32      99

  Fragments arranged by size: 

                 449     196     169      99      73      54      32      32
                  32      32      32
 
KasI G'GCGC_C

Cuts at:      0     830    1200 
   Size:        830     370
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     724    1200 
   Size:        724     476
 
MscI TGG'CCA

Cuts at:      0       4    1132    1200 
   Size:          4    1128      68

  Fragments arranged by size: 

                1128      68       4
 
MslI CAynn'nnrTG

Cuts at:      0     301    1200 
   Size:        301     899
 
MspA1I CmG'CkG

Cuts at:      0     647    1200 
   Size:        647     553
 
NaeI GCC'GGC

Cuts at:      0     835    1200 
   Size:        835     365
 
NarI GG'CG_CC

Cuts at:      0     831    1200 
   Size:        831     369
 
NcoI C'CATG_G

Cuts at:      0     509     563    1200 
   Size:        509      54     637

  Fragments arranged by size: 

                 637     509      54
 
NgoMIV G'CCGG_C

Cuts at:      0     833    1200 
   Size:        833     367
 
NheI G'CTAG_C

Cuts at:      0     463    1200 
   Size:        463     737
 
NspI r_CATG'y

Cuts at:      0     765    1200 
   Size:        765     435
 
PpuMI rG'GwC_Cy

Cuts at:      0     924    1200 
   Size:        924     276
 
PshAI GACnn'nnGTC

Cuts at:      0    1047    1200 
   Size:       1047     153
 
PspOMI G'GGCC_C

Cuts at:      0     857    1200 
   Size:        857     343
 
SacI G_AGCT'C

Cuts at:      0     124    1200 
   Size:        124    1076
 
SfoI GGC'GCC

Cuts at:      0     832    1200 
   Size:        832     368
 
SgrAI Cr'CCGG_yG

Cuts at:      0     833    1200 
   Size:        833     367
 
SphI G_CATG'C

Cuts at:      0     765    1200 
   Size:        765     435
 
StyI C'CwwG_G

Cuts at:      0      21     509     563    1200 
   Size:         21     488      54     637

  Fragments arranged by size: 

                 637     488      54      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      93    1200 
   Size:         93    1107
 
TatI w'GTAC_w

Cuts at:      0     131    1200 
   Size:        131    1069
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI     ApaI    BamHI     BanI    BanII     BbeI
    BbvCI     BglI     BlpI Bme1580I     BmrI     BmtI     BpmI   Bpu10I
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BsrFI    BsrGI    BssSI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NspI
    PpuMI    PshAI   PspOMI     SacI     SfoI    SgrAI     SphI     StyI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BbsI     BcgI    BciVI     BclI
    BfrBI    BglII    BmgBI     BplI    BpuEI    BsaAI    BsaBI    BsiEI
    BsiWI     BsmI    BspHI    BspMI    BsrBI    BsrDI   BssHII   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     ClaI     DraI     DrdI     EagI
     EarI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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