Restriction map for
pFN21AB9856
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI AlwNI AocI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlpI BpmI Bpu1102I BsiWI BsmBI
BsmFI BspDI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI Eam1105I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI MfeI
MluI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 628 GTCTAC GT^[AC][GT]AC
AccIII 841 TCCGGA T^CCGGA
Alw44I 1161 GTGCAC G^TGCAC
Aor51HI 746 AGCGCT AGC^GCT
ApaLI 1161 GTGCAC G^TGCAC
AvrII 945 CCTAGG C^CTAGG
BalI 182 TGGCCA TGG^CCA
BbsI 523 GTCTTC GAAGAC(2/6)
BglI 265 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 945 CCTAGG C^CTAGG
BpuAI 523 GTCTTC GAAGAC(2/6)
BsaAI 694 TACGTA [CT]AC^GT[GA]
BsaI 49 GAGACC GGTCTC(1/5)
BseAI 841 TCCGGA T^CCGGA
BsiEI 336 CGGCCG CG[GA][CT]^CG
Bsp1407I 323 TGTACA T^GTACA
BspEI 841 TCCGGA T^CCGGA
BspHI 503 TCATGA T^CATGA
BspMI 284 ACCTGC ACCTGC(4/8)
BsrBI 705 CCGCTC CCGCTC(-3/-3)
BsrGI 323 TGTACA T^GTACA
BstYI 844 GGATCT [GA]^GATC[CT]
DraI 1126 TTTAAA TTT^AAA
EagI 336 CGGCCG C^GGCCG
EarI 577 GAAGAG CTCTTC(1/4)
EclXI 336 CGGCCG C^GGCCG
Eco47III 746 AGCGCT AGC^GCT
Eco52I 336 CGGCCG C^GGCCG
Ksp632I 577 GAAGAG CTCTTC(1/4)
KspI 38 CCGCGG CCGC^GG
MflI 844 GGATCT [GA]^GATC[CT]
MluNI 182 TGGCCA TGG^CCA
MroI 841 TCCGGA T^CCGGA
MscI 182 TGGCCA TGG^CCA
PstI 96 CTGCAG CTGCA^G
PvuII 763 CAGCTG CAG^CTG
RcaI 503 TCATGA T^CATGA
SacII 38 CCGCGG CCGC^GG
SfcI 96 CTGCAG C^T[GA][CT]AG
SmaI 781 CCCGGG CCC^GGG
SmlI 62 CTCAAG C^T[CT][GA]AG
SnaBI 694 TACGTA TAC^GTA
SspBI 323 TGTACA T^GTACA
TfiI 838 GAATC G^A[AT]TC
XhoII 844 GGATCT [GA]^GATC[CT]
XmaCI 781 CCCGGG C^CCGGG
XmaI 781 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AluI 474 AGCT AG^CT
764 AGCT
AlwI 844 GGATC GGATC(4/5)
950 GGATC
AspHI 14 GTGCTC G[AT]GC[AT]^C
1161 GTGCAC
AvaI 755 CCCGAG C^[CT]CG[GA]G
781 CCCGGG
BanII 42 GGGCTC G[GA]GC[CT]^C
758 GAGCCC
BbvI 98 GCAGC GCAGC(8/12)
543 GCTGC
BsaBI 306 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1008 GATGCCTATC
BsaWI 224 ACCGGA [AT]^CCGG[AT]
841 TCCGGA
BseRI 667 CTCCTC GAGGAG(10/8)
580 GAGGAG
BsgI 238 CTGCAC GTGCAG(16/14)
412 CTGCAC
BsiHKAI 14 GTGCTC G[AT]GC[AT]^C
1161 GTGCAC
BsmAI 49 GAGAC GTCTC(1/5)
880 GTCTC
BsmI 592 GAATGC GAATGC(1/-1)
1135 GCATTC
BsoBI 755 CCCGAG C^[CT]CG[GA]G
781 CCCGGG
BsrFI 334 ACCGGC [GA]^CCGG[CT]
739 ACCGGC
BsrI 348 ACTGG ACTGG(1/-1)
443 ACTGG
BstEII 276 GGTCACC G^GT[ACGT]ACC
798 GGTCACC
BstPI 276 GGTCACC G^GT[ACGT]ACC
798 GGTCACC
Cfr10I 334 ACCGGC [GA]^CCGG[CT]
739 ACCGGC
EcoO65I 276 GGTCACC G^GT[ACGT]ACC
798 GGTCACC
MamI 306 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1008 GATGCCTATC
MslI 297 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
415 CACCTCTGTG
ScaI 147 AGTACT AGT^ACT
467 AGTACT
TseI 98 GCAGC G^C[AT]GC
543 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AfaI AvaII
BcnI BfaI BmyI BsaHI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I Tsp45I
TspRI