(Circular) (Six-base) MAPSORT of: pFN21AB9851.seq Check: 8648 from: 1 to: 5845 pFN21AB9851 5845 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3188 1937 Size: 1251 4594 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2912 3042 3908 5292 5560 1632 Size: 1280 130 866 1384 268 1917 Fragments arranged by size: 1917 1384 1280 866 268 130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 4744 5721 1203 Size: 3541 977 1327 Fragments arranged by size: 3541 1327 977 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 2878 4574 2878 Size: 1696 4149 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2450 2585 1184 Size: 1266 135 4444 Fragments arranged by size: 4444 1266 135 AlwNI CAG_nnn'CTG Cuts at: 2131 2650 2882 3588 5160 2131 Size: 519 232 706 1572 2816 Fragments arranged by size: 2816 1572 706 519 232 ApaI G_GGCC'C Cuts at: 2733 2733 Size: 5845 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 2010 2378 2621 3154 3380 3532 5641 1493 Size: 517 368 243 533 226 152 2109 1697 Fragments arranged by size: 2109 1697 533 517 368 243 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 3170 1870 Size: 73 1227 4545 Fragments arranged by size: 4545 1227 73 BamHI G'GATC_C Cuts at: 3089 3175 3542 3089 Size: 86 367 5392 Fragments arranged by size: 5392 367 86 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3166 4621 618 Size: 325 217 627 1379 1455 1842 Fragments arranged by size: 1842 1455 1379 627 325 217 BanII G_rGCy'C Cuts at: 1391 2733 3164 1391 Size: 1342 431 4072 Fragments arranged by size: 4072 1342 431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4874 BbvCI CC'TCA_GC Cuts at: 2319 2319 Size: 5845 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4036 4070 1903 Size: 34 2099 34 3678 Fragments arranged by size: 3678 2099 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2726 3737 4947 2726 Size: 1011 1210 3624 Fragments arranged by size: 3624 1210 1011 BclI T'GATC_A Cuts at: 1548 2922 1548 Size: 1374 4471 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2448 3003 4456 Size: 107 122 71 631 605 776 555 1453 Cuts at: 4456 136 Size: 1525 Fragments arranged by size: 1525 1453 776 631 605 555 122 107 71 BglII A'GATC_T Cuts at: 5840 5840 Size: 5845 BlpI GC'TnA_GC Cuts at: 2438 3063 3262 2438 Size: 625 199 5021 Fragments arranged by size: 5021 625 199 Bme1580I G_kGCm'C Cuts at: 1790 2733 3906 5062 1790 Size: 943 1173 1156 2573 Fragments arranged by size: 2573 1173 1156 943 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2043 2466 3123 Size: 416 53 83 399 24 626 423 657 Cuts at: 3123 3576 4534 442 Size: 453 958 1753 Fragments arranged by size: 1753 958 657 626 453 423 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4505 1239 Size: 3266 2579 Bpu10I CC'TnA_GC Cuts at: 2319 2346 2630 2319 Size: 27 284 5534 Fragments arranged by size: 5534 284 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2264 3970 4835 5133 2264 Size: 1706 865 298 2976 Fragments arranged by size: 2976 1706 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2174 4508 882 Size: 617 675 2334 2219 Fragments arranged by size: 2334 2219 675 617 BsaAI yAC'GTr Cuts at: 493 1204 3636 493 Size: 711 2432 2702 Fragments arranged by size: 2702 2432 711 BsaBI GATnn'nnATC Cuts at: 5839 5839 Size: 5845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3652 4034 4669 275 Size: 53 83 186 936 2119 382 635 1451 Fragments arranged by size: 2119 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4278 4676 4950 5097 5508 4278 Size: 398 274 147 411 4615 Fragments arranged by size: 4615 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2879 2909 1178 Size: 30 165 30 1476 30 4114 Fragments arranged by size: 4114 1476 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2789 2972 1186 Size: 1000 603 183 4059 Fragments arranged by size: 4059 1000 603 183 BseYI C'CCAG_C Cuts at: 2354 2650 5048 5544 2354 Size: 296 2398 496 2655 Fragments arranged by size: 2655 2398 496 296 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3295 4056 5084 1540 Size: 275 186 1294 761 1028 2301 Fragments arranged by size: 2301 1294 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3906 3991 5062 3906 Size: 85 1071 4689 Fragments arranged by size: 4689 1071 85 BsmI GAATG_Cn' Cuts at: 3356 3449 3356 Size: 93 5752 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2733 3164 3906 3991 5062 1391 Size: 399 943 431 742 85 1071 2174 Fragments arranged by size: 2174 1071 943 742 431 399 85 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 3734 5464 3734 Size: 1730 4115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3200 844 Size: 2356 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2311 3732 1394 Size: 429 488 1421 3507 Fragments arranged by size: 3507 1421 488 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4340 4514 65 Size: 1183 3092 174 1396 Fragments arranged by size: 3092 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3044 4489 4676 1700 Size: 110 1234 1445 187 2869 Fragments arranged by size: 2869 1445 1234 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5845 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5845 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 3040 1184 Size: 1856 3989 BstXI CCAn_nnnn'nTGG Cuts at: 2823 2823 Size: 5845 BstYI r'GATC_y Cuts at: 1972 2677 3089 3175 3542 3927 3944 5396 5482 Size: 705 412 86 367 385 17 1452 86 Cuts at: 5482 5494 5840 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 705 412 385 367 346 86 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2862 2862 Size: 5845 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4354 Fragments arranged by size: 4354 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5106 Fragments arranged by size: 5106 683 56 BtsI GCAGTG_nn' Cuts at: 2311 2704 2977 3361 4155 4175 2311 Size: 393 273 384 794 20 3981 Fragments arranged by size: 3981 794 393 384 273 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3133 3149 3133 Size: 16 5829 DraIII CAC_nnn'GTG Cuts at: 2474 2474 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2086 2097 Size: 54 1425 50 275 18 192 64 11 Cuts at: 2097 2295 3292 4181 5810 8 Size: 198 997 889 1629 43 Fragments arranged by size: 1629 1425 997 889 275 198 192 64 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 3292 1537 Size: 275 1480 4090 Fragments arranged by size: 4090 1480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2855 2910 3061 3775 1353 Size: 1502 55 151 714 3423 Fragments arranged by size: 3423 1502 714 151 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2912 3042 3908 4505 5292 5560 1239 Size: 393 1280 130 866 597 787 268 1524 Fragments arranged by size: 1524 1280 866 787 597 393 268 130 EcoNI CCTnn'n_nnAGG Cuts at: 2504 2660 2504 Size: 156 5689 EcoRI G'AATT_C Cuts at: 2010 2621 3154 3532 2010 Size: 611 533 378 4323 Fragments arranged by size: 4323 611 533 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2098 2098 Size: 5845 FspI TGC'GCA Cuts at: 4351 4351 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 4992 1678 Size: 3314 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2107 2139 2864 2896 4486 Size: 32 135 32 535 32 725 32 1590 Cuts at: 4486 4518 4560 4592 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1590 725 535 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3189 3435 669 Size: 988 281 1251 246 3079 Fragments arranged by size: 3079 1251 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4959 5143 1305 Size: 3654 184 2007 Fragments arranged by size: 3654 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2024 2099 2297 10 Size: 54 1425 343 192 75 198 3558 Fragments arranged by size: 3558 1425 343 198 192 75 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2418 3803 4162 4321 518 Size: 648 1056 196 1385 359 159 2042 Fragments arranged by size: 2042 1385 1056 648 359 196 159 MspA1I CmG'CkG Cuts at: 1606 2335 2490 3261 3938 5086 5331 1606 Size: 729 155 771 677 1148 245 2120 Fragments arranged by size: 2120 1148 771 729 677 245 155 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4354 Fragments arranged by size: 4354 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 2206 3203 4748 1132 Size: 97 977 997 1545 2229 Fragments arranged by size: 2229 1545 997 977 97 PasI CC'CwG_GG Cuts at: 1348 3013 1348 Size: 1665 4180 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 2197 1108 Size: 1089 4756 PfoI T'CCnGG_A Cuts at: 2058 2616 2058 Size: 558 5287 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PspOMI G'GGCC_C Cuts at: 2729 2729 Size: 5845 PstI C_TGCA'G Cuts at: 830 2166 2340 2646 3197 830 Size: 1336 174 306 551 3478 Fragments arranged by size: 3478 1336 551 306 174 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 2335 2490 2335 Size: 155 5690 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SapI GCTCTTCn'nnn_ Cuts at: 2855 2910 2855 Size: 55 5790 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SfcI C'TryA_G Cuts at: 826 1045 2162 2336 2642 3193 4328 5009 5200 Size: 219 1117 174 306 551 1135 681 191 Cuts at: 5200 826 Size: 1471 Fragments arranged by size: 1471 1135 1117 681 551 306 219 191 174 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2279 3949 4850 5112 820 Size: 197 926 336 1670 901 262 1553 Fragments arranged by size: 1670 1553 926 901 336 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 2019 1517 Size: 502 5343 StyI C'CwwG_G Cuts at: 513 1065 2004 3001 3084 3284 513 Size: 552 939 997 83 200 3074 Fragments arranged by size: 3074 997 939 552 200 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2485 3026 3872 3889 4042 4227 4672 Size: 1255 905 541 846 17 153 185 445 Cuts at: 4672 325 Size: 1498 Fragments arranged by size: 1498 1255 905 846 541 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4091 5803 96 Size: 274 80 33 51 1443 2114 1712 138 Fragments arranged by size: 2114 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2879 2911 5805 5837 2879 Size: 32 2894 32 2887 Fragments arranged by size: 2894 2887 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2194 2224 2740 2194 Size: 30 516 5299 Fragments arranged by size: 5299 516 30 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI