Restriction map for pFN21AB9850
 (Circular) (Six-base) MAPSORT of: pFN21AB9850.seq  Check: 1193  from: 1  to: 5836



pFN21AB9850  5836 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:44  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3646     278 
   Size:         53      83     186     936    2110    2468

  Fragments arranged by size: 

                2468    2110     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3179    1937 
   Size:       1242    4594
 
Acc65I G'GTAC_C

Cuts at:   3157    3157 
   Size:       5836
 
AclI AA'CG_TT

Cuts at:   1563    3963    4336    1563 
   Size:       2400     373    3063

  Fragments arranged by size: 

                3063    2400     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3899    5283    5551    1632 
   Size:       2267    1384     268    1917

  Fragments arranged by size: 

                2267    1917    1384     268
 
AfeI AGC'GCT

Cuts at:   2717    2717 
   Size:       5836
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5639
 
AflIII A'CryG_T

Cuts at:   1203    4735    5712    1203 
   Size:       3532     977    1327

  Fragments arranged by size: 

                3532    1327     977
 
AgeI A'CCGG_T

Cuts at:   4667    4667 
   Size:       5836
 
AhdI GACnn_n'nnGTC

Cuts at:   4565    4565 
   Size:       5836
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5836
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5836
 
AlwNI CAG_nnn'CTG

Cuts at:   2415    2747    3579    5151    2415 
   Size:        332     832    1572    3100

  Fragments arranged by size: 

                3100    1572     832     332
 
ApaLI G'TGCA_C

Cuts at:   3893    5049    3893 
   Size:       1156    4680
 
ApoI r'AATT_y

Cuts at:   1493    3145    3371    3523    5632    1493 
   Size:       1652     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1652     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4390     160 
   Size:        872    3358    1606

  Fragments arranged by size: 

                3358    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5836
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2886    3161    1870 
   Size:         73     943     275    4545

  Fragments arranged by size: 

                4545     943     275      73
 
BamHI G'GATC_C

Cuts at:   2092    3000    3166    3533    2092 
   Size:        908     166     367    4395

  Fragments arranged by size: 

                4395     908     367     166
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3157    4612     618 
   Size:        325     217     627    1370    1455    1842

  Fragments arranged by size: 

                1842    1455    1370     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3155    1391 
   Size:       1764    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4865
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4027    4061    1903 
   Size:         34    2090      34    3678

  Fragments arranged by size: 

                3678    2090      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3728    4938    3728 
   Size:       1210    4626
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5836
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4447     136 
   Size:        107     122      71     631     605    2775    1525

  Fragments arranged by size: 

                2775    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2118    2586    5831    2118 
   Size:        468    3245    2123

  Fragments arranged by size: 

                3245    2123     468
 
BlpI GC'TnA_GC

Cuts at:   3253    3253 
   Size:       5836
 
Bme1580I G_kGCm'C

Cuts at:   1790    2796    2860    3897    5053    1790 
   Size:       1006      64    1037    1156    2573

  Fragments arranged by size: 

                2573    1156    1037    1006      64
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5836
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2425    3567    4525 
   Size:        416      53      83     399      24    1008    1142     958

Cuts at:   4525     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1142    1008     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5836
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2812    2812 
   Size:       5836
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2090    2761    4496    1239 
   Size:        851     671    1735    2579

  Fragments arranged by size: 

                2579    1735     851     671
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2099    3961    4826    5124    2099 
   Size:       1862     865     298    2811

  Fragments arranged by size: 

                2811    1862     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4499     882 
   Size:        617    3000    2219

  Fragments arranged by size: 

                3000    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2364    2833    3627     493 
   Size:        711    1160     469     794    2702

  Fragments arranged by size: 

                2702    1160     794     711     469
 
BsaBI GATnn'nnATC

Cuts at:   5830    5830 
   Size:       5836
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3643    4025    4660     275 
   Size:         53      83     186     936    2110     382     635    1451

  Fragments arranged by size: 

                2110    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3123    4269    4667    4941    5088    5499    3123 
   Size:       1146     398     274     147     411    3460

  Fragments arranged by size: 

                3460    1146     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2543    2573    1178 
   Size:         30     165      30    1140      30    4441

  Fragments arranged by size: 

                4441    1140     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2567    1186 
   Size:       1381    4455
 
BseYI C'CCAG_C

Cuts at:   5039    5535    5039 
   Size:        496    5340
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2353    1163 
   Size:        573     617    4646

  Fragments arranged by size: 

                4646     617     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3286    4047    5075    1540 
   Size:        275     186    1285     761    1028    2301

  Fragments arranged by size: 

                2301    1285    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3897    3982    5053    3897 
   Size:         85    1071    4680

  Fragments arranged by size: 

                4680    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2071    3347    3440    2071 
   Size:       1276      93    4467

  Fragments arranged by size: 

                4467    1276      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2796    2860    3155    3897    3982    5053    1391 
   Size:        399    1006      64     295     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071    1006     742     399     295      85      64
            
 
BspEI T'CCGG_A

Cuts at:   3123    5499    3123 
   Size:       2376    3460
 
BspHI T'CATG_A

Cuts at:   3725    5455    3725 
   Size:       1730    4106
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3191     844 
   Size:       2347    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2524    3723    1394 
   Size:        429     701    1199    3507

  Fragments arranged by size: 

                3507    1199     701     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2397    4331    4505      65 
   Size:       1183    1149    1934     174    1396

  Fragments arranged by size: 

                1934    1396    1183    1149     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4480    4667    1700 
   Size:        110    2670     187    2869

  Fragments arranged by size: 

                2869    2670     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5836
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5836
 
BssSI C'ACGA_G

Cuts at:   3896    4908    3896 
   Size:       1012    4824
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5836
 
BstBI TT'CG_AA

Cuts at:   4640    4640 
   Size:       5836
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5836
 
BstYI r'GATC_y

Cuts at:   1972    2057    2092    2118    2586    2661    3000    3166    3533 
   Size:         85      35      26     468      75     339     166     367

Cuts at:   3533    3918    3935    5387    5473    5485    5831    1972 
   Size:        385      17    1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452     468     385     367     346     339     166
                  86      85      75      35      26      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2130    2724     513 
   Size:        552    1065     594    3625

  Fragments arranged by size: 

                3625    1065     594     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2275     487 
   Size:        683      56    1049    4048

  Fragments arranged by size: 

                4048    1049     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3352    4146    4166    3352 
   Size:        794      20    5022

  Fragments arranged by size: 

                5022     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5836
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5801
 
DraI TTT'AAA

Cuts at:   3045    3140    3045 
   Size:         95    5741
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4843     809 
   Size:        507    3527    1802

  Fragments arranged by size: 

                3527    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2154    2244    2323 
   Size:         54    1425      50     275      18     324      90      79

Cuts at:   2323    2461    2469    2656    3283    4172    5801       8 
   Size:        138       8     187     627     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     627     324     275     187     138
                  90      79      54      50      43      18       8
 
EagI C'GGCC_G

Cuts at:   1537    1812    3283    1537 
   Size:        275    1471    4090

  Fragments arranged by size: 

                4090    1471     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2531    3081    3766    1353 
   Size:       1178     550     685    3423

  Fragments arranged by size: 

                3423    1178     685     550
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4423    4797    4943     387 
   Size:       1064     138    2834     374     146    1280

  Fragments arranged by size: 

                2834    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2090    2761    3899    4496    5283    5551    1239 
   Size:        393     458     671    1138     597     787     268    1524

  Fragments arranged by size: 

                1524    1138     787     671     597     458     393     268
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2039    2786    2039 
   Size:        747    5089
 
EcoO109I rG'GnC_Cy

Cuts at:   2100    2616    2754    2928    2100 
   Size:        516     138     174    5008

  Fragments arranged by size: 

                5008     516     174     138
 
EcoRI G'AATT_C

Cuts at:   3145    3523    3145 
   Size:        378    5458
 
FspI TGC'GCA

Cuts at:   4342    4342 
   Size:       5836
 
HaeII r_GCGC'y

Cuts at:   1678    2114    2719    3014    4983    1678 
   Size:        436     605     295    1969    2531

  Fragments arranged by size: 

                2531    1969     605     436     295
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2543    2575    4477    4509    4551 
   Size:         32     135      32     971      32    1902      32      42

Cuts at:   4551    4583    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1902     971     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3180    3426     669 
   Size:        988     281    1242     246    3079

  Fragments arranged by size: 

                3079    1242     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2843    3099     748 
   Size:       2095     256    3485

  Fragments arranged by size: 

                3485    2095     256
 
HpaI GTT'AAC

Cuts at:   3426    3426 
   Size:       5836
 
KpnI G_GTAC'C

Cuts at:   3161    3161 
   Size:       5836
 
MfeI C'AATT_G

Cuts at:   3435    3435 
   Size:       5836
 
MluI A'CGCG_T

Cuts at:   5712    5712 
   Size:       5836
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2076    2541    4950    5134    1305 
   Size:        771     465    2409     184    2007

  Fragments arranged by size: 

                2409    2007     771     465     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2156    2246    2325    2463      10 
   Size:         54    1425     343     324      90      79     138    3383

  Fragments arranged by size: 

                3383    1425     343     324     138      90      79      54
            
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2391    3794    4153    4312     518 
   Size:        648    1225    1403     359     159    2042

  Fragments arranged by size: 

                2042    1403    1225     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3252    3929    5077    5322    1606 
   Size:       1646     677    1148     245    2120

  Fragments arranged by size: 

                2120    1646    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5284
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5836
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5836
 
NotI GC'GGCC_GC

Cuts at:   3283    3283 
   Size:       5836
 
NspI r_CATG'y

Cuts at:   1132    1229    3194    4739    1132 
   Size:         97    1965    1545    2229

  Fragments arranged by size: 

                2229    1965    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5836
 
PciI A'CATG_T

Cuts at:   4735    4735 
   Size:       5836
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2490    1108 
   Size:       1382    4454
 
PmeI GTTT'AAAC

Cuts at:   3140    3140 
   Size:       5836
 
PmlI CAC'GTG

Cuts at:   2364    2833    2364 
   Size:        469    5367
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3905    3937    5418    5450    3905 
   Size:         32    1481      32    4291

  Fragments arranged by size: 

                4291    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2100    2754    2100 
   Size:        654    5182
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5836
 
PsiI TTA'TAA

Cuts at:   3406    3406 
   Size:       5836
 
PstI C_TGCA'G

Cuts at:    830    3188     830 
   Size:       2358    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5836
 
SalI G'TCGA_C

Cuts at:   1936    3178    1936 
   Size:       1242    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2531    2531 
   Size:       5836
 
SbfI CC_TGCA'GG

Cuts at:   3188    3188 
   Size:       5836
 
ScaI AGT'ACT

Cuts at:   4084    5796    4084 
   Size:       1712    4124
 
SfcI C'TryA_G

Cuts at:    826    1045    3184    4319    5000    5191     826 
   Size:        219    2139    1135     681     191    1471

  Fragments arranged by size: 

                2139    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5836
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5836
 
SmaI CCC'GGG

Cuts at:   1872    3163    1872 
   Size:       1291    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2114    2886    3940    4841    5103     820 
   Size:        197     926     171     772    1054     901     262    1553

  Fragments arranged by size: 

                1553    1054     926     901     772     262     197     171
            
 
SnaBI TAC'GTA

Cuts at:    493    3627     493 
   Size:       3134    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5836
 
SphI G_CATG'C

Cuts at:   1132    3194    1132 
   Size:       2062    3774
 
SspI AAT'ATT

Cuts at:      5      52    3760       5 
   Size:         47    3708    2081

  Fragments arranged by size: 

                3708    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2034    2048    3131    1517 
   Size:        517      14    1083    4222

  Fragments arranged by size: 

                4222    1083     517      14
 
StyI C'CwwG_G

Cuts at:    513    1065    2541    3275     513 
   Size:        552    1476     734    3074

  Fragments arranged by size: 

                3074    1476     734     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2321    3863    3880    4033    4218    4663     325 
   Size:       1255     741    1542      17     153     185     445    1498

  Fragments arranged by size: 

                1542    1498    1255     741     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2179    4082    5794 
   Size:        274      80      33      51    1443     202    1903    1712

Cuts at:   5794      96 
   Size:        138

  Fragments arranged by size: 

                1903    1712    1443     274     202     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5796    5828    5796 
   Size:         32    5804
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5836
 
XbaI T'CTAG_A

Cuts at:   3172    3172 
   Size:       5836
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2257    2908    2257 
   Size:        651    5185
 
XhoI C'TCGA_G

Cuts at:   1943    2886    1943 
   Size:        943    4893
 
XmaI C'CCGG_G

Cuts at:   1870    3161    1870 
   Size:       1291    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2034    2717    3090    3965    2034 
   Size:        683     373     875    3905

  Fragments arranged by size: 

                3905     875     683     373
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3644     276 
   Size:         53      83     186     936    2110    2468

  Fragments arranged by size: 

                2468    2110     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI     PstI
     PvuI     SalI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI   PspOMI    PspXI     PsrI
    PvuII    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SfoI
     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com