(Circular) (Six-base) MAPSORT of: pFN21AB9850.seq Check: 1193 from: 1 to: 5836 pFN21AB9850 5836 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3899 5283 5551 1632 Size: 2267 1384 268 1917 Fragments arranged by size: 2267 1917 1384 268 AfeI AGC'GCT Cuts at: 2717 2717 Size: 5836 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 2415 2747 3579 5151 2415 Size: 332 832 1572 3100 Fragments arranged by size: 3100 1572 832 332 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 3145 3371 3523 5632 1493 Size: 1652 226 152 2109 1697 Fragments arranged by size: 2109 1697 1652 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2886 3161 1870 Size: 73 943 275 4545 Fragments arranged by size: 4545 943 275 73 BamHI G'GATC_C Cuts at: 2092 3000 3166 3533 2092 Size: 908 166 367 4395 Fragments arranged by size: 4395 908 367 166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3157 4612 618 Size: 325 217 627 1370 1455 1842 Fragments arranged by size: 1842 1455 1370 627 325 217 BanII G_rGCy'C Cuts at: 1391 3155 1391 Size: 1764 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4865 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4027 4061 1903 Size: 34 2090 34 3678 Fragments arranged by size: 3678 2090 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4447 136 Size: 107 122 71 631 605 2775 1525 Fragments arranged by size: 2775 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2118 2586 5831 2118 Size: 468 3245 2123 Fragments arranged by size: 3245 2123 468 BlpI GC'TnA_GC Cuts at: 3253 3253 Size: 5836 Bme1580I G_kGCm'C Cuts at: 1790 2796 2860 3897 5053 1790 Size: 1006 64 1037 1156 2573 Fragments arranged by size: 2573 1156 1037 1006 64 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2425 3567 4525 Size: 416 53 83 399 24 1008 1142 958 Cuts at: 4525 442 Size: 1753 Fragments arranged by size: 1753 1142 1008 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2812 2812 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2761 4496 1239 Size: 851 671 1735 2579 Fragments arranged by size: 2579 1735 851 671 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2099 3961 4826 5124 2099 Size: 1862 865 298 2811 Fragments arranged by size: 2811 1862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4499 882 Size: 617 3000 2219 Fragments arranged by size: 3000 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2364 2833 3627 493 Size: 711 1160 469 794 2702 Fragments arranged by size: 2702 1160 794 711 469 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3643 4025 4660 275 Size: 53 83 186 936 2110 382 635 1451 Fragments arranged by size: 2110 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3123 4269 4667 4941 5088 5499 3123 Size: 1146 398 274 147 411 3460 Fragments arranged by size: 3460 1146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 1178 Size: 30 165 30 1140 30 4441 Fragments arranged by size: 4441 1140 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2567 1186 Size: 1381 4455 BseYI C'CCAG_C Cuts at: 5039 5535 5039 Size: 496 5340 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2353 1163 Size: 573 617 4646 Fragments arranged by size: 4646 617 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3897 3982 5053 3897 Size: 85 1071 4680 Fragments arranged by size: 4680 1071 85 BsmI GAATG_Cn' Cuts at: 2071 3347 3440 2071 Size: 1276 93 4467 Fragments arranged by size: 4467 1276 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2796 2860 3155 3897 3982 5053 1391 Size: 399 1006 64 295 742 85 1071 2174 Fragments arranged by size: 2174 1071 1006 742 399 295 85 64 BspEI T'CCGG_A Cuts at: 3123 5499 3123 Size: 2376 3460 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3191 844 Size: 2347 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2524 3723 1394 Size: 429 701 1199 3507 Fragments arranged by size: 3507 1199 701 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2397 4331 4505 65 Size: 1183 1149 1934 174 1396 Fragments arranged by size: 1934 1396 1183 1149 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4480 4667 1700 Size: 110 2670 187 2869 Fragments arranged by size: 2869 2670 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5836 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstYI r'GATC_y Cuts at: 1972 2057 2092 2118 2586 2661 3000 3166 3533 Size: 85 35 26 468 75 339 166 367 Cuts at: 3533 3918 3935 5387 5473 5485 5831 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 468 385 367 346 339 166 86 85 75 35 26 17 12 BtgI C'CryG_G Cuts at: 513 1065 2130 2724 513 Size: 552 1065 594 3625 Fragments arranged by size: 3625 1065 594 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2275 487 Size: 683 56 1049 4048 Fragments arranged by size: 4048 1049 683 56 BtsI GCAGTG_nn' Cuts at: 3352 4146 4166 3352 Size: 794 20 5022 Fragments arranged by size: 5022 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3045 3140 3045 Size: 95 5741 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 2244 2323 Size: 54 1425 50 275 18 324 90 79 Cuts at: 2323 2461 2469 2656 3283 4172 5801 8 Size: 138 8 187 627 889 1629 43 Fragments arranged by size: 1629 1425 889 627 324 275 187 138 90 79 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2531 3081 3766 1353 Size: 1178 550 685 3423 Fragments arranged by size: 3423 1178 685 550 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4423 4797 4943 387 Size: 1064 138 2834 374 146 1280 Fragments arranged by size: 2834 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2761 3899 4496 5283 5551 1239 Size: 393 458 671 1138 597 787 268 1524 Fragments arranged by size: 1524 1138 787 671 597 458 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2039 2786 2039 Size: 747 5089 EcoO109I rG'GnC_Cy Cuts at: 2100 2616 2754 2928 2100 Size: 516 138 174 5008 Fragments arranged by size: 5008 516 174 138 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2114 2719 3014 4983 1678 Size: 436 605 295 1969 2531 Fragments arranged by size: 2531 1969 605 436 295 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2543 2575 4477 4509 4551 Size: 32 135 32 971 32 1902 32 42 Cuts at: 4551 4583 1373 Size: 32 2626 Fragments arranged by size: 2626 1902 971 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 2843 3099 748 Size: 2095 256 3485 Fragments arranged by size: 3485 2095 256 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2076 2541 4950 5134 1305 Size: 771 465 2409 184 2007 Fragments arranged by size: 2409 2007 771 465 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2156 2246 2325 2463 10 Size: 54 1425 343 324 90 79 138 3383 Fragments arranged by size: 3383 1425 343 324 138 90 79 54 MslI CAynn'nnrTG Cuts at: 518 1166 2391 3794 4153 4312 518 Size: 648 1225 1403 359 159 2042 Fragments arranged by size: 2042 1403 1225 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3252 3929 5077 5322 1606 Size: 1646 677 1148 245 2120 Fragments arranged by size: 2120 1646 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5284 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5836 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 2490 1108 Size: 1382 4454 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PmlI CAC'GTG Cuts at: 2364 2833 2364 Size: 469 5367 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2100 2754 2100 Size: 654 5182 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 3188 830 Size: 2358 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SapI GCTCTTCn'nnn_ Cuts at: 2531 2531 Size: 5836 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 3184 4319 5000 5191 826 Size: 219 2139 1135 681 191 1471 Fragments arranged by size: 2139 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2114 2886 3940 4841 5103 820 Size: 197 926 171 772 1054 901 262 1553 Fragments arranged by size: 1553 1054 926 901 772 262 197 171 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 2034 2048 3131 1517 Size: 517 14 1083 4222 Fragments arranged by size: 4222 1083 517 14 StyI C'CwwG_G Cuts at: 513 1065 2541 3275 513 Size: 552 1476 734 3074 Fragments arranged by size: 3074 1476 734 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 3863 3880 4033 4218 4663 325 Size: 1255 741 1542 17 153 185 445 1498 Fragments arranged by size: 1542 1498 1255 741 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2179 4082 5794 Size: 274 80 33 51 1443 202 1903 1712 Cuts at: 5794 96 Size: 138 Fragments arranged by size: 1903 1712 1443 274 202 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5796 5828 5796 Size: 32 5804 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2908 2257 Size: 651 5185 XhoI C'TCGA_G Cuts at: 1943 2886 1943 Size: 943 4893 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 2034 2717 3090 3965 2034 Size: 683 373 875 3905 Fragments arranged by size: 3905 875 683 373 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI