Restriction map for pFN21AB9849
 (Circular) (Six-base) MAPSORT of: pFN21AB9849.seq  Check: 5159  from: 1  to: 5836



pFN21AB9849  5836 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:44  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2387    2387 
   Size:       5836
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3646     278 
   Size:         53      83     186     936    2110    2468

  Fragments arranged by size: 

                2468    2110     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3179    1937 
   Size:       1242    4594
 
Acc65I G'GTAC_C

Cuts at:   3157    3157 
   Size:       5836
 
AclI AA'CG_TT

Cuts at:   1563    3963    4336    1563 
   Size:       2400     373    3063

  Fragments arranged by size: 

                3063    2400     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2290    2563    3899    5283    5551    1632 
   Size:        658     273    1336    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1336     658     273     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5639
 
AflIII A'CryG_T

Cuts at:   1203    4735    5712    1203 
   Size:       3532     977    1327

  Fragments arranged by size: 

                3532    1327     977
 
AgeI A'CCGG_T

Cuts at:   2394    4667    2394 
   Size:       2273    3563
 
AhdI GACnn_n'nnGTC

Cuts at:   4565    4565 
   Size:       5836
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5836
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2345    2405    2750    1184 
   Size:       1161      60     345    4270

  Fragments arranged by size: 

                4270    1161     345      60
 
AlwNI CAG_nnn'CTG

Cuts at:   3579    5151    3579 
   Size:       1572    4264
 
ApaI G_GGCC'C

Cuts at:   2024    2446    2024 
   Size:        422    5414
 
ApaLI G'TGCA_C

Cuts at:   2390    3893    5049    2390 
   Size:       1503    1156    3177

  Fragments arranged by size: 

                3177    1503    1156
 
ApoI r'AATT_y

Cuts at:   1493    3145    3371    3523    5632    1493 
   Size:       1652     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1652     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4390     160 
   Size:        872    3358    1606

  Fragments arranged by size: 

                3358    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5836
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2570    2701    2955    3161    1870 
   Size:         73     627     131     254     206    4545

  Fragments arranged by size: 

                4545     627     254     206     131      73
 
AvrII C'CTAG_G

Cuts at:   2714    2714 
   Size:       5836
 
BamHI G'GATC_C

Cuts at:   3166    3533    3166 
   Size:        367    5469
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2045    2076    2566    2629    3157 
   Size:        325     217     627     258      31     490      63     528

Cuts at:   3157    4612     618 
   Size:       1455    1842

  Fragments arranged by size: 

                1842    1455     627     528     490     325     258     217
                  63      31
 
BanII G_rGCy'C

Cuts at:   1391    2024    2446    2577    3155    1391 
   Size:        633     422     131     578    4072

  Fragments arranged by size: 

                4072     633     578     422     131
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2755     928 
   Size:        971     856    4009

  Fragments arranged by size: 

                4009     971     856
 
BbvCI CC'TCA_GC

Cuts at:   2831    2831 
   Size:       5836
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4027    4061    1903 
   Size:         34    2090      34    3678

  Fragments arranged by size: 

                3678    2090      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3728    4938    3728 
   Size:       1210    4626
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5836
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2498    2996    4447 
   Size:        107     122      71     631     605     826     498    1451

Cuts at:   4447     136 
   Size:       1525

  Fragments arranged by size: 

                1525    1451     826     631     605     498     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5831    5831 
   Size:       5836
 
BlpI GC'TnA_GC

Cuts at:   3253    3253 
   Size:       5836
 
Bme1580I G_kGCm'C

Cuts at:   1790    2024    2394    2446    2634    3897    5053    1790 
   Size:        234     370      52     188    1263    1156    2573

  Fragments arranged by size: 

                2573    1263    1156     370     234     188      52
 
BmgBI CAC'GTC

Cuts at:   1318    2791    1318 
   Size:       1473    4363
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3567    4525     442 
   Size:        416      53      83     399      24    2150     958    1753

  Fragments arranged by size: 

                2150    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5836
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4496    1239 
   Size:       3257    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2831    2831 
   Size:       5836
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2174    3961    4826    5124    2174 
   Size:       1787     865     298    2886

  Fragments arranged by size: 

                2886    1787     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4499     882 
   Size:        617    3000    2219

  Fragments arranged by size: 

                3000    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2104    2659    3627     493 
   Size:        711     900     555     968    2702

  Fragments arranged by size: 

                2702     968     900     711     555
 
BsaBI GATnn'nnATC

Cuts at:   5830    5830 
   Size:       5836
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2406    3643    4025    4660 
   Size:         53      83     186     936     873    1237     382     635

Cuts at:   4660     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1237     936     873     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2394    4269    4667    4941    5088    5499    2394 
   Size:       1875     398     274     147     411    2731

  Fragments arranged by size: 

                2731    1875     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5611

  Fragments arranged by size: 

                5611     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5836
 
BseYI C'CCAG_C

Cuts at:   2065    2433    2611    2930    2981    3010    5039    5535    2065 
   Size:        368     178     319      51      29    2029     496    2366

  Fragments arranged by size: 

                2366    2029     496     368     319     178      51      29
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5263
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2882    3286    4047    5075    1540 
   Size:        275     186     881     404     761    1028    2301

  Fragments arranged by size: 

                2301    1028     881     761     404     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2319    2394    2511    2577    2664    2919    3897    3982    5053 
   Size:         75     117      66      87     255     978      85    1071

Cuts at:   5053    2319 
   Size:       3102

  Fragments arranged by size: 

                3102    1071     978     255     117      87      85      75
                  66
 
BsiWI C'GTAC_G

Cuts at:   2944    2944 
   Size:       5836
 
BsmI GAATG_Cn'

Cuts at:   2206    3347    3440    2206 
   Size:       1141      93    4602

  Fragments arranged by size: 

                4602    1141      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2024    2319    2394    2446    2511    2577    2634 
   Size:        399     234     295      75      52      65      66      57

Cuts at:   2634    2664    2919    3155    3897    3982    5053    1391 
   Size:         30     255     236     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     295     255     236     234
                  85      75      66      65      57      52      30
 
BspEI T'CCGG_A

Cuts at:   5499    5499 
   Size:       5836
 
BspHI T'CATG_A

Cuts at:   3725    5455    3725 
   Size:       1730    4106
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2387    2558    3191     844 
   Size:       1543     171     633    3489

  Fragments arranged by size: 

                3489    1543     633     171
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3723    1394 
   Size:        429    1900    3507

  Fragments arranged by size: 

                3507    1900     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4331    4505      65 
   Size:       1183    3083     174    1396

  Fragments arranged by size: 

                3083    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2394    3100    4480    4667    1700 
   Size:        110     584     706    1380     187    2869

  Fragments arranged by size: 

                2869    1380     706     584     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5836
 
BssHII G'CGCG_C

Cuts at:   1925    2529    2531    1925 
   Size:        604       2    5230

  Fragments arranged by size: 

                5230     604       2
 
BssSI C'ACGA_G

Cuts at:   3896    4908    3896 
   Size:       1012    4824
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2249     794 
   Size:       1455    4381
 
BstBI TT'CG_AA

Cuts at:   4640    4640 
   Size:       5836
 
BstEII G'GTnAC_C

Cuts at:   1184    2872    1184 
   Size:       1688    4148
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2749    2860    2749 
   Size:        111    5725
 
BstYI r'GATC_y

Cuts at:   1972    3023    3166    3533    3918    3935    5387    5473    5485 
   Size:       1051     143     367     385      17    1452      86      12

Cuts at:   5485    5831    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452    1051     385     367     346     143      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2160    2287    2748     513 
   Size:        552     939     156     127     461    3601

  Fragments arranged by size: 

                3601     939     552     461     156     127
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2728     487 
   Size:        683      56    1502    3595

  Fragments arranged by size: 

                3595    1502     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3076    3352    4146    4166    3076 
   Size:        276     794      20    4746

  Fragments arranged by size: 

                4746     794     276      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5836
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5801
 
DraI TTT'AAA

Cuts at:   3140    3140 
   Size:       5836
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4843     809 
   Size:        507    3527    1802

  Fragments arranged by size: 

                3527    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2463    2879    2886 
   Size:         54    1425      50     275      18     633     416       7

Cuts at:   2886    3283    4172    5801       8 
   Size:        397     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     633     416     397     275      54
                  50      43      18       7
 
EagI C'GGCC_G

Cuts at:   1537    1812    2879    3283    1537 
   Size:        275    1067     404    4090

  Fragments arranged by size: 

                4090    1067     404     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2345    2375    2582    2627    3766    1353 
   Size:        992      30     207      45    1139    3423

  Fragments arranged by size: 

                3423    1139     992     207      45      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3100    4423    4797    4943     387 
   Size:       1064     138    1511    1323     374     146    1280

  Fragments arranged by size: 

                1511    1323    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2575    2575 
   Size:       5836
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2290    2563    3899    4496    5283    5551    1239 
   Size:        393     658     273    1336     597     787     268    1524

  Fragments arranged by size: 

                1524    1336     787     658     597     393     273     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2021    2500    2697    2021 
   Size:        479     197    5160

  Fragments arranged by size: 

                5160     479     197
 
EcoRI G'AATT_C

Cuts at:   3145    3523    3145 
   Size:        378    5458
 
FspI TGC'GCA

Cuts at:   2738    3020    4342    2738 
   Size:        282    1322    4232

  Fragments arranged by size: 

                4232    1322     282
 
FspAI rTGC'GCAy

Cuts at:   2738    2738 
   Size:       5836
 
HaeII r_GCGC'y

Cuts at:   1678    2168    4983    1678 
   Size:        490    2815    2531

  Fragments arranged by size: 

                2815    2531     490
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4477    4509    4551    4583    1373 
   Size:         32     135      32    2905      32      42      32    2626

  Fragments arranged by size: 

                2905    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3180    3426     669 
   Size:        988     281    1242     246    3079

  Fragments arranged by size: 

                3079    1242     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5836
 
HpaI GTT'AAC

Cuts at:   3426    3426 
   Size:       5836
 
KpnI G_GTAC'C

Cuts at:   3161    3161 
   Size:       5836
 
MfeI C'AATT_G

Cuts at:   3435    3435 
   Size:       5836
 
MluI A'CGCG_T

Cuts at:   5712    5712 
   Size:       5836
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3051    4950    5134    1305 
   Size:       1746    1899     184    2007

  Fragments arranged by size: 

                2007    1899    1746     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2888      10 
   Size:         54    1425     343    1056    2958

  Fragments arranged by size: 

                2958    1425    1056     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2216    2387    2747    2858    3794    4153    4312 
   Size:        648    1050     171     360     111     936     359     159

Cuts at:   4312     518 
   Size:       2042

  Fragments arranged by size: 

                2042    1050     936     648     360     359     171     159
                 111
 
MspA1I CmG'CkG

Cuts at:   1606    2266    2289    2835    2884    3252    3929    5077    5322 
   Size:        660      23     546      49     368     677    1148     245

Cuts at:   5322    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     677     660     546     368     245      49
                  23
 
NaeI GCC'GGC

Cuts at:   3102    3102 
   Size:       5836
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4345

  Fragments arranged by size: 

                4345     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5836
 
NgoMIV G'CCGG_C

Cuts at:   3100    3100 
   Size:       5836
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5836
 
NotI GC'GGCC_GC

Cuts at:   3283    3283 
   Size:       5836
 
NspI r_CATG'y

Cuts at:   1132    1229    3043    3194    4739    1132 
   Size:         97    1814     151    1545    2229

  Fragments arranged by size: 

                2229    1814    1545     151      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5836
 
PciI A'CATG_T

Cuts at:   4735    4735 
   Size:       5836
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2975    1108 
   Size:       1867    3969
 
PmeI GTTT'AAAC

Cuts at:   3140    3140 
   Size:       5836
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3905    3937    5418    5450    3905 
   Size:         32    1481      32    4291

  Fragments arranged by size: 

                4291    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2697    2697 
   Size:       5836
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5836
 
PsiI TTA'TAA

Cuts at:   3406    3406 
   Size:       5836
 
PspOMI G'GGCC_C

Cuts at:   2020    2442    2020 
   Size:        422    5414
 
PstI C_TGCA'G

Cuts at:    830    2363    3008    3188     830 
   Size:       1533     645     180    3478

  Fragments arranged by size: 

                3478    1533     645     180
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5836
 
PvuII CAG'CTG

Cuts at:   2835    2835 
   Size:       5836
 
SacI G_AGCT'C

Cuts at:   2577    2577 
   Size:       5836
 
SacII CC_GC'GG

Cuts at:   2290    2290 
   Size:       5836
 
SalI G'TCGA_C

Cuts at:   1936    3178    1936 
   Size:       1242    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2582    2627    2582 
   Size:         45    5791
 
SbfI CC_TGCA'GG

Cuts at:   3188    3188 
   Size:       5836
 
ScaI AGT'ACT

Cuts at:   4084    5796    4084 
   Size:       1712    4124
 
SfcI C'TryA_G

Cuts at:    826    1045    2071    2359    2596    3004    3184    4319    5000 
   Size:        219    1026     288     237     408     180    1135     681

Cuts at:   5000    5191     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1135    1026     681     408     288     237     219
                 191     180
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2498    2498 
   Size:       5836
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5836
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    2394    1700 
   Size:        694    5142
 
SmaI CCC'GGG

Cuts at:   1872    3163    1872 
   Size:       1291    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2189    3940    4841    5103     820 
   Size:        197     926     246    1751     901     262    1553

  Fragments arranged by size: 

                1751    1553     926     901     262     246     197
 
SnaBI TAC'GTA

Cuts at:    493    3627     493 
   Size:       3134    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5836
 
SphI G_CATG'C

Cuts at:   1132    3194    1132 
   Size:       2062    3774
 
SspI AAT'ATT

Cuts at:      5      52    3760       5 
   Size:         47    3708    2081

  Fragments arranged by size: 

                3708    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5836
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2714    3275     513 
   Size:        552     939     710     561    3074

  Fragments arranged by size: 

                3074     939     710     561     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2467    2986    3863    3880    4033    4218    4663 
   Size:       1255     887     519     877      17     153     185     445

Cuts at:   4663     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255     887     877     519     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4082    5794      96 
   Size:        274      80      33      51    1443    2105    1712     138

  Fragments arranged by size: 

                2105    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5796    5828    5796 
   Size:         32    5804
 
Tth111I GACn'n_nGTC

Cuts at:   1316    3091    1316 
   Size:       1775    4061
 
XbaI T'CTAG_A

Cuts at:   3172    3172 
   Size:       5836
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5836
 
XmaI C'CCGG_G

Cuts at:   1870    3161    1870 
   Size:       1291    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3965    3965 
   Size:       5836
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3644     276 
   Size:         53      83     186     936    2110    2468

  Fragments arranged by size: 

                2468    2110     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI
     PvuI    PvuII     SacI    SacII     SalI     SapI     SbfI     ScaI
     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI     BaeI     BbeI    BfrBI     BplI    BsmBI
  BstZ17I   Bsu36I   DraIII    EcoNI    EcoRV     FalI     FseI     KasI
     NarI     NruI     NsiI     PacI     PfoI     PmlI    PspXI     PsrI
    RsrII    SanDI    SexAI     SfoI     SrfI     SwaI     XcmI
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