(Circular) (Six-base) MAPSORT of: pFN21AB9849.seq Check: 5159 from: 1 to: 5836 pFN21AB9849 5836 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2387 2387 Size: 5836 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2290 2563 3899 5283 5551 1632 Size: 658 273 1336 1384 268 1917 Fragments arranged by size: 1917 1384 1336 658 273 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 2394 4667 2394 Size: 2273 3563 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2345 2405 2750 1184 Size: 1161 60 345 4270 Fragments arranged by size: 4270 1161 345 60 AlwNI CAG_nnn'CTG Cuts at: 3579 5151 3579 Size: 1572 4264 ApaI G_GGCC'C Cuts at: 2024 2446 2024 Size: 422 5414 ApaLI G'TGCA_C Cuts at: 2390 3893 5049 2390 Size: 1503 1156 3177 Fragments arranged by size: 3177 1503 1156 ApoI r'AATT_y Cuts at: 1493 3145 3371 3523 5632 1493 Size: 1652 226 152 2109 1697 Fragments arranged by size: 2109 1697 1652 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2570 2701 2955 3161 1870 Size: 73 627 131 254 206 4545 Fragments arranged by size: 4545 627 254 206 131 73 AvrII C'CTAG_G Cuts at: 2714 2714 Size: 5836 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2076 2566 2629 3157 Size: 325 217 627 258 31 490 63 528 Cuts at: 3157 4612 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 528 490 325 258 217 63 31 BanII G_rGCy'C Cuts at: 1391 2024 2446 2577 3155 1391 Size: 633 422 131 578 4072 Fragments arranged by size: 4072 633 578 422 131 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2755 928 Size: 971 856 4009 Fragments arranged by size: 4009 971 856 BbvCI CC'TCA_GC Cuts at: 2831 2831 Size: 5836 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4027 4061 1903 Size: 34 2090 34 3678 Fragments arranged by size: 3678 2090 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2498 2996 4447 Size: 107 122 71 631 605 826 498 1451 Cuts at: 4447 136 Size: 1525 Fragments arranged by size: 1525 1451 826 631 605 498 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 3253 3253 Size: 5836 Bme1580I G_kGCm'C Cuts at: 1790 2024 2394 2446 2634 3897 5053 1790 Size: 234 370 52 188 1263 1156 2573 Fragments arranged by size: 2573 1263 1156 370 234 188 52 BmgBI CAC'GTC Cuts at: 1318 2791 1318 Size: 1473 4363 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3567 4525 442 Size: 416 53 83 399 24 2150 958 1753 Fragments arranged by size: 2150 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4496 1239 Size: 3257 2579 Bpu10I CC'TnA_GC Cuts at: 2831 2831 Size: 5836 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 3961 4826 5124 2174 Size: 1787 865 298 2886 Fragments arranged by size: 2886 1787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4499 882 Size: 617 3000 2219 Fragments arranged by size: 3000 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2104 2659 3627 493 Size: 711 900 555 968 2702 Fragments arranged by size: 2702 968 900 711 555 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2406 3643 4025 4660 Size: 53 83 186 936 873 1237 382 635 Cuts at: 4660 275 Size: 1451 Fragments arranged by size: 1451 1237 936 873 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2394 4269 4667 4941 5088 5499 2394 Size: 1875 398 274 147 411 2731 Fragments arranged by size: 2731 1875 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5611 Fragments arranged by size: 5611 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5836 BseYI C'CCAG_C Cuts at: 2065 2433 2611 2930 2981 3010 5039 5535 2065 Size: 368 178 319 51 29 2029 496 2366 Fragments arranged by size: 2366 2029 496 368 319 178 51 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5263 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2882 3286 4047 5075 1540 Size: 275 186 881 404 761 1028 2301 Fragments arranged by size: 2301 1028 881 761 404 275 186 BsiHKAI G_wGCw'C Cuts at: 2319 2394 2511 2577 2664 2919 3897 3982 5053 Size: 75 117 66 87 255 978 85 1071 Cuts at: 5053 2319 Size: 3102 Fragments arranged by size: 3102 1071 978 255 117 87 85 75 66 BsiWI C'GTAC_G Cuts at: 2944 2944 Size: 5836 BsmI GAATG_Cn' Cuts at: 2206 3347 3440 2206 Size: 1141 93 4602 Fragments arranged by size: 4602 1141 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2319 2394 2446 2511 2577 2634 Size: 399 234 295 75 52 65 66 57 Cuts at: 2634 2664 2919 3155 3897 3982 5053 1391 Size: 30 255 236 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 295 255 236 234 85 75 66 65 57 52 30 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2387 2558 3191 844 Size: 1543 171 633 3489 Fragments arranged by size: 3489 1543 633 171 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4331 4505 65 Size: 1183 3083 174 1396 Fragments arranged by size: 3083 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2394 3100 4480 4667 1700 Size: 110 584 706 1380 187 2869 Fragments arranged by size: 2869 1380 706 584 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 2529 2531 1925 Size: 604 2 5230 Fragments arranged by size: 5230 604 2 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 2249 794 Size: 1455 4381 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 2872 1184 Size: 1688 4148 BstXI CCAn_nnnn'nTGG Cuts at: 2749 2860 2749 Size: 111 5725 BstYI r'GATC_y Cuts at: 1972 3023 3166 3533 3918 3935 5387 5473 5485 Size: 1051 143 367 385 17 1452 86 12 Cuts at: 5485 5831 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1051 385 367 346 143 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2160 2287 2748 513 Size: 552 939 156 127 461 3601 Fragments arranged by size: 3601 939 552 461 156 127 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2728 487 Size: 683 56 1502 3595 Fragments arranged by size: 3595 1502 683 56 BtsI GCAGTG_nn' Cuts at: 3076 3352 4146 4166 3076 Size: 276 794 20 4746 Fragments arranged by size: 4746 794 276 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2463 2879 2886 Size: 54 1425 50 275 18 633 416 7 Cuts at: 2886 3283 4172 5801 8 Size: 397 889 1629 43 Fragments arranged by size: 1629 1425 889 633 416 397 275 54 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 2879 3283 1537 Size: 275 1067 404 4090 Fragments arranged by size: 4090 1067 404 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2345 2375 2582 2627 3766 1353 Size: 992 30 207 45 1139 3423 Fragments arranged by size: 3423 1139 992 207 45 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3100 4423 4797 4943 387 Size: 1064 138 1511 1323 374 146 1280 Fragments arranged by size: 1511 1323 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2575 2575 Size: 5836 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2290 2563 3899 4496 5283 5551 1239 Size: 393 658 273 1336 597 787 268 1524 Fragments arranged by size: 1524 1336 787 658 597 393 273 268 EcoO109I rG'GnC_Cy Cuts at: 2021 2500 2697 2021 Size: 479 197 5160 Fragments arranged by size: 5160 479 197 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FspI TGC'GCA Cuts at: 2738 3020 4342 2738 Size: 282 1322 4232 Fragments arranged by size: 4232 1322 282 FspAI rTGC'GCAy Cuts at: 2738 2738 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2168 4983 1678 Size: 490 2815 2531 Fragments arranged by size: 2815 2531 490 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373 Size: 32 135 32 2905 32 42 32 2626 Fragments arranged by size: 2905 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3051 4950 5134 1305 Size: 1746 1899 184 2007 Fragments arranged by size: 2007 1899 1746 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2888 10 Size: 54 1425 343 1056 2958 Fragments arranged by size: 2958 1425 1056 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2387 2747 2858 3794 4153 4312 Size: 648 1050 171 360 111 936 359 159 Cuts at: 4312 518 Size: 2042 Fragments arranged by size: 2042 1050 936 648 360 359 171 159 111 MspA1I CmG'CkG Cuts at: 1606 2266 2289 2835 2884 3252 3929 5077 5322 Size: 660 23 546 49 368 677 1148 245 Cuts at: 5322 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 660 546 368 245 49 23 NaeI GCC'GGC Cuts at: 3102 3102 Size: 5836 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4345 Fragments arranged by size: 4345 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NgoMIV G'CCGG_C Cuts at: 3100 3100 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3043 3194 4739 1132 Size: 97 1814 151 1545 2229 Fragments arranged by size: 2229 1814 1545 151 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5836 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 2975 1108 Size: 1867 3969 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2697 2697 Size: 5836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PspOMI G'GGCC_C Cuts at: 2020 2442 2020 Size: 422 5414 PstI C_TGCA'G Cuts at: 830 2363 3008 3188 830 Size: 1533 645 180 3478 Fragments arranged by size: 3478 1533 645 180 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2835 2835 Size: 5836 SacI G_AGCT'C Cuts at: 2577 2577 Size: 5836 SacII CC_GC'GG Cuts at: 2290 2290 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SapI GCTCTTCn'nnn_ Cuts at: 2582 2627 2582 Size: 45 5791 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 2071 2359 2596 3004 3184 4319 5000 Size: 219 1026 288 237 408 180 1135 681 Cuts at: 5000 5191 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1026 681 408 288 237 219 191 180 SfiI GGCCn_nnn'nGGCC Cuts at: 2498 2498 Size: 5836 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 2394 1700 Size: 694 5142 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 3940 4841 5103 820 Size: 197 926 246 1751 901 262 1553 Fragments arranged by size: 1751 1553 926 901 262 246 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 2004 2714 3275 513 Size: 552 939 710 561 3074 Fragments arranged by size: 3074 939 710 561 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2467 2986 3863 3880 4033 4218 4663 Size: 1255 887 519 877 17 153 185 445 Cuts at: 4663 325 Size: 1498 Fragments arranged by size: 1498 1255 887 877 519 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4082 5794 96 Size: 274 80 33 51 1443 2105 1712 138 Fragments arranged by size: 2105 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5796 5828 5796 Size: 32 5804 Tth111I GACn'n_nGTC Cuts at: 1316 3091 1316 Size: 1775 4061 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BfrBI BplI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfoI SrfI SwaI XcmI