Restriction map for
pFN21AB9849
(Linear) (Six-base) MAPSORT of: KIBB9849.seq Check: 6341 from: 1 to: 1131
KIBB9849.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 382 1131
Size: 382 749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 558 1131
Size: 285 273 573
Fragments arranged by size:
573 285 273
AgeI A'CCGG_T
Cuts at: 0 389 1131
Size: 389 742
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 340 400 745 1131
Size: 340 60 345 386
Fragments arranged by size:
386 345 340 60
ApaI G_GGCC'C
Cuts at: 0 19 441 1131
Size: 19 422 690
Fragments arranged by size:
690 422 19
ApaLI G'TGCA_C
Cuts at: 0 385 1131
Size: 385 746
AvaI C'yCGr_G
Cuts at: 0 565 696 950 1131
Size: 565 131 254 181
Fragments arranged by size:
565 254 181 131
AvrII C'CTAG_G
Cuts at: 0 709 1131
Size: 709 422
BanI G'GyrC_C
Cuts at: 0 40 71 561 624 1131
Size: 40 31 490 63 507
Fragments arranged by size:
507 490 63 40 31
BanII G_rGCy'C
Cuts at: 0 19 441 572 1131
Size: 19 422 131 559
Fragments arranged by size:
559 422 131 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 750 1131
Size: 750 381
BbvCI CC'TCA_GC
Cuts at: 0 826 1131
Size: 826 305
BglI GCCn_nnn'nGGC
Cuts at: 0 493 991 1131
Size: 493 498 140
Fragments arranged by size:
498 493 140
Bme1580I G_kGCm'C
Cuts at: 0 19 389 441 629 1131
Size: 19 370 52 188 502
Fragments arranged by size:
502 370 188 52 19
BmgBI CAC'GTC
Cuts at: 0 786 1131
Size: 786 345
Bpu10I CC'TnA_GC
Cuts at: 0 826 1131
Size: 826 305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1131
Size: 169 962
BsaAI yAC'GTr
Cuts at: 0 99 654 1131
Size: 99 555 477
Fragments arranged by size:
555 477 99
BsaHI Gr'CG_yC
Cuts at: 0 401 1131
Size: 401 730
BsaWI w'CCGG_w
Cuts at: 0 389 1131
Size: 389 742
BseYI C'CCAG_C
Cuts at: 0 60 428 606 925 976 1005 1131
Size: 60 368 178 319 51 29 126
Fragments arranged by size:
368 319 178 126 60 51 29
BsiEI CG_ry'CG
Cuts at: 0 877 1131
Size: 877 254
BsiHKAI G_wGCw'C
Cuts at: 0 314 389 506 572 659 914 1131
Size: 314 75 117 66 87 255 217
Fragments arranged by size:
314 255 217 117 87 75 66
BsiWI C'GTAC_G
Cuts at: 0 939 1131
Size: 939 192
BsmI GAATG_Cn'
Cuts at: 0 201 1131
Size: 201 930
Bsp1286I G_dGCh'C
Cuts at: 0 19 314 389 441 506 572 629 659
Size: 19 295 75 52 65 66 57 30
Cuts at: 659 914 1131
Size: 255 217
Fragments arranged by size:
295 255 217 75 66 65 57 52
30 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 382 553 1131
Size: 382 171 578
Fragments arranged by size:
578 382 171
BsrFI r'CCGG_y
Cuts at: 0 389 1095 1131
Size: 389 706 36
Fragments arranged by size:
706 389 36
BssHII G'CGCG_C
Cuts at: 0 524 526 1131
Size: 524 2 605
Fragments arranged by size:
605 524 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 244 1131
Size: 244 887
BstEII G'GTnAC_C
Cuts at: 0 867 1131
Size: 867 264
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 855 1131
Size: 744 111 276
Fragments arranged by size:
744 276 111
BstYI r'GATC_y
Cuts at: 0 1018 1131
Size: 1018 113
BtgI C'CryG_G
Cuts at: 0 155 282 743 1131
Size: 155 127 461 388
Fragments arranged by size:
461 388 155 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 723 1131
Size: 723 408
BtsI GCAGTG_nn'
Cuts at: 0 1071 1131
Size: 1071 60
EaeI y'GGCC_r
Cuts at: 0 458 874 881 1131
Size: 458 416 7 250
Fragments arranged by size:
458 416 250 7
EagI C'GGCC_G
Cuts at: 0 874 1131
Size: 874 257
EarI CTCTTCn'nnn_
Cuts at: 0 340 370 577 622 1131
Size: 340 30 207 45 509
Fragments arranged by size:
509 340 207 45 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1095 1131
Size: 1095 36
EcoICRI GAG'CTC
Cuts at: 0 570 1131
Size: 570 561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 558 1131
Size: 285 273 573
Fragments arranged by size:
573 285 273
EcoO109I rG'GnC_Cy
Cuts at: 0 16 495 692 1131
Size: 16 479 197 439
Fragments arranged by size:
479 439 197 16
FspI TGC'GCA
Cuts at: 0 733 1015 1131
Size: 733 282 116
Fragments arranged by size:
733 282 116
FspAI rTGC'GCAy
Cuts at: 0 733 1131
Size: 733 398
HaeII r_GCGC'y
Cuts at: 0 163 1131
Size: 163 968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 1131
Size: 1046 85
MscI TGG'CCA
Cuts at: 0 883 1131
Size: 883 248
MslI CAynn'nnrTG
Cuts at: 0 211 382 742 853 1131
Size: 211 171 360 111 278
Fragments arranged by size:
360 278 211 171 111
MspA1I CmG'CkG
Cuts at: 0 261 284 830 879 1131
Size: 261 23 546 49 252
Fragments arranged by size:
546 261 252 49 23
NaeI GCC'GGC
Cuts at: 0 1097 1131
Size: 1097 34
NgoMIV G'CCGG_C
Cuts at: 0 1095 1131
Size: 1095 36
NspI r_CATG'y
Cuts at: 0 1038 1131
Size: 1038 93
PflMI CCAn_nnn'nTGG
Cuts at: 0 970 1131
Size: 970 161
PpuMI rG'GwC_Cy
Cuts at: 0 692 1131
Size: 692 439
PspOMI G'GGCC_C
Cuts at: 0 15 437 1131
Size: 15 422 694
Fragments arranged by size:
694 422 15
PstI C_TGCA'G
Cuts at: 0 358 1003 1131
Size: 358 645 128
Fragments arranged by size:
645 358 128
PvuII CAG'CTG
Cuts at: 0 830 1131
Size: 830 301
SacI G_AGCT'C
Cuts at: 0 572 1131
Size: 572 559
SacII CC_GC'GG
Cuts at: 0 285 1131
Size: 285 846
SapI GCTCTTCn'nnn_
Cuts at: 0 577 622 1131
Size: 577 45 509
Fragments arranged by size:
577 509 45
SfcI C'TryA_G
Cuts at: 0 66 354 591 999 1131
Size: 66 288 237 408 132
Fragments arranged by size:
408 288 237 132 66
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 493 1131
Size: 493 638
SgrAI Cr'CCGG_yG
Cuts at: 0 389 1131
Size: 389 742
SmlI C'TyrA_G
Cuts at: 0 184 1131
Size: 184 947
StyI C'CwwG_G
Cuts at: 0 709 1131
Size: 709 422
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 462 981 1131
Size: 462 519 150
Fragments arranged by size:
519 462 150
Tth111I GACn'n_nGTC
Cuts at: 0 1086 1131
Size: 1086 45
Enzymes that do cut:
AarI AcuI AgeI AlfI ApaI ApaLI AvaI AvrII
BanI BanII BbsI BbvCI BglI Bme1580I BmgBI Bpu10I
BpuEI BsaAI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspMI BsrFI BssHII BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I FspI FspAI HaeII MmeI MscI
MslI MspA1I NaeI NgoMIV NspI PflMI PpuMI PspOMI
PstI PvuII SacI SacII SapI SfcI SfiI SgrAI
SmlI StyI TaqII Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AloI AlwNI ApoI AscI AseI AsiSI BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BplI BpmI BsaI BsaBI BsaXI BseRI
BsgI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI
BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EcoNI EcoRI EcoRV FalI FseI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SbfI ScaI SexAI SfoI SgfI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI