Restriction map for
pFN21AB9847
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanII
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MroI
MseI MunI NaeI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 298 GTCTAC GT^[AC][GT]AC
AccII 736 CGCG CG^CG
AcyI 716 GGCGCC G[GA]^CG[CT]C
AlwI 320 GGATC GGATC(4/5)
AlwNI 283 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1095 CCTGAGG CC^T[ACGT]AGG
Asp700I 543 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 716 GGCGCC GGCGC^C
BglI 241 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 716 GGCGCC G[GA]^CG[CT]C
BsmFI 1092 GTCCC GGGAC(10/14)
BsrFI 713 ACCGGC [GA]^CCGG[CT]
BssSI 400 CTCGTG CACGAG(-5/-1)
BstEII 172 GGTAACC G^GT[ACGT]ACC
BstPI 172 GGTAACC G^GT[ACGT]ACC
BstUI 736 CGCG CG^CG
BstYI 320 GGATCT [GA]^GATC[CT]
Bsu36I 1095 CCTGAGG CC^T[ACGT]AGG
Cfr10I 713 ACCGGC [GA]^CCGG[CT]
DraIII 44 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 115 GACGTGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 658 CTCTTC CTCTTC(1/4)
Eco81I 1095 CCTGAGG CC^T[ACGT]AGG
EcoO65I 172 GGTAACC G^GT[ACGT]ACC
HapII 714 CCGG C^CGG
HgaI 947 GCGTC GACGC(5/10)
Hin1I 716 GGCGCC G[GA]^CG[CT]C
HincII 949 GTCAAC GT[CT]^[GA]AC
HindII 949 GTCAAC GT[CT]^[GA]AC
HpaII 714 CCGG C^CGG
KasI 716 GGCGCC G^GCGCC
Ksp632I 658 CTCTTC CTCTTC(1/4)
MflI 320 GGATCT [GA]^GATC[CT]
MspA1I 286 CCGCTG C[AC]G^C[GT]G
MspI 714 CCGG C^CGG
MvnI 736 CGCG CG^CG
NarI 716 GGCGCC GG^CGCC
NcoI 932 CCATGG C^CATGG
PshAI 291 GACCGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 721 CTGCAG CTGCA^G
ScaI 665 AGTACT AGT^ACT
SfoI 716 GGCGCC GGC^GCC
StuI 464 AGGCCT AGG^CCT
TfiI 106 GATTC G^A[AT]TC
XcmI 527 CCAACAGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 320 GGATCT [GA]^GATC[CT]
XmnI 543 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AspHI 481 GTGCTC G[AT]GC[AT]^C
826 GTGCTC
BalI 524 TGGCCA TGG^CCA
613 TGGCCA
BbvI 27 GCTGC GCAGC(8/12)
834 GCTGC
BsaI 331 GAGACC GGTCTC(1/5)
378 GGTCTC
BsiHKAI 481 GTGCTC G[AT]GC[AT]^C
826 GTGCTC
BsrDI 97 CATTGC GCAATG(2/0)
495 CATTGC
BstXI 436 CCAACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
566 CCAAGGCTCTGG
DpnII 321 GATC ^GATC
996 GATC
EaeI 524 TGGCCA [CT]^GGCC[GA]
613 TGGCCA
EcoT14I 566 CCAAGG C^C[AT][AT]GG
932 CCATGG
HaeII 716 GGCGCC [GA]GCGC^[CT]
1027 AGCGCC
MaeII 116 ACGT A^CGT
221 ACGT
MboI 321 GATC ^GATC
996 GATC
MluNI 524 TGGCCA TGG^CCA
613 TGGCCA
MscI 524 TGGCCA TGG^CCA
613 TGGCCA
MslI 303 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
366 CATGTCGGTG
NdeII 321 GATC ^GATC
996 GATC
NspI 365 GCATGT [GA]CATG^[CT]
843 GCATGT
PleI 607 GAGTC GAGTC(4/5)
1042 GAGTC
PpuMI 164 GGGTCCT [GA]G^G[AT]CC[CT]
1090 GGGTCCC
Sau3AI 321 GATC ^GATC
996 GATC
StyI 566 CCAAGG C^C[AT][AT]GG
932 CCATGG
TseI 27 GCTGC G^C[AT]GC
834 GCTGC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbsI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EcoO109I EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeIII MboII MnlI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI SfcI Tsp45I TspRI