(Circular) (Six-base) MAPSORT of: pFN21AB9847.seq Check: 2218 from: 1 to: 5830 pFN21AB9847 5830 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2957 2957 Size: 5830 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2304 3173 1937 Size: 367 869 4594 Fragments arranged by size: 4594 869 367 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3893 5277 5545 1632 Size: 2261 1384 268 1917 Fragments arranged by size: 2261 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AlwNI CAG_nnn'CTG Cuts at: 2293 3573 5145 2293 Size: 1280 1572 2978 Fragments arranged by size: 2978 1572 1280 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 3139 3365 3517 5626 1493 Size: 1646 226 152 2109 1697 Fragments arranged by size: 2109 1697 1646 226 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 3155 1870 Size: 73 1212 4545 Fragments arranged by size: 4545 1212 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2721 2756 2874 3151 Size: 325 217 627 291 643 35 118 277 Cuts at: 3151 4606 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 643 627 325 291 277 217 118 35 BanII G_rGCy'C Cuts at: 1391 3149 1391 Size: 1758 4072 BbeI G_GCGC'C Cuts at: 2725 2725 Size: 5830 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2122 2545 2874 928 Size: 971 223 423 329 3884 Fragments arranged by size: 3884 971 423 329 223 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4021 4055 1903 Size: 34 2084 34 3678 Fragments arranged by size: 3678 2084 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2252 4441 136 Size: 107 122 71 631 605 580 2189 1525 Fragments arranged by size: 2189 1525 631 605 580 122 107 71 BglII A'GATC_T Cuts at: 5825 5825 Size: 5830 BlpI GC'TnA_GC Cuts at: 3247 3247 Size: 5830 Bme1580I G_kGCm'C Cuts at: 1790 2081 2759 2781 3891 5047 1790 Size: 291 678 22 1110 1156 2573 Fragments arranged by size: 2573 1156 1110 678 291 22 BmgBI CAC'GTC Cuts at: 1318 2122 1318 Size: 804 5026 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2693 3561 4519 Size: 416 53 83 399 24 1276 868 958 Cuts at: 4519 442 Size: 1753 Fragments arranged by size: 1753 1276 958 868 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2357 2357 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2435 2564 2600 3062 4490 1239 Size: 1196 129 36 462 1428 2579 Fragments arranged by size: 2579 1428 1196 462 129 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3955 4820 5118 3955 Size: 865 298 4667 Fragments arranged by size: 4667 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2330 2389 4493 882 Size: 617 831 59 2104 2219 Fragments arranged by size: 2219 2104 831 617 59 BsaAI yAC'GTr Cuts at: 493 1204 3621 493 Size: 711 2417 2702 Fragments arranged by size: 2702 2417 711 BsaBI GATnn'nnATC Cuts at: 5824 5824 Size: 5830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2722 3637 4019 4654 Size: 53 83 186 936 1189 915 382 635 Cuts at: 4654 275 Size: 1451 Fragments arranged by size: 1451 1189 936 915 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5605 Fragments arranged by size: 5605 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2651 3059 1186 Size: 1084 381 408 3957 Fragments arranged by size: 3957 1084 408 381 BseYI C'CCAG_C Cuts at: 2456 2774 5033 5529 2456 Size: 318 2259 496 2757 Fragments arranged by size: 2757 2259 496 318 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5257 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4041 5069 1540 Size: 275 186 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2490 2835 3891 3976 5047 2490 Size: 345 1056 85 1071 3273 Fragments arranged by size: 3273 1071 1056 345 85 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2490 2759 2781 2835 3149 3891 Size: 399 291 409 269 22 54 314 742 Cuts at: 3891 3976 5047 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 409 399 314 291 269 85 54 22 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 2779 2957 3105 3185 844 Size: 1400 535 178 148 80 3489 Fragments arranged by size: 3489 1400 535 178 148 80 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2101 2499 4325 4499 65 Size: 1183 853 398 1826 174 1396 Fragments arranged by size: 1826 1396 1183 853 398 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2718 4474 4661 1700 Size: 110 908 1756 187 2869 Fragments arranged by size: 2869 1756 908 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 2405 3890 4902 2405 Size: 1485 1012 3333 Fragments arranged by size: 3333 1485 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5830 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 2177 1184 Size: 993 4837 BstXI CCAn_nnnn'nTGG Cuts at: 2448 2578 2448 Size: 130 5700 BstYI r'GATC_y Cuts at: 1972 2325 3160 3527 3912 3929 5381 5467 5479 Size: 353 835 367 385 17 1452 86 12 Cuts at: 5479 5825 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 835 385 367 353 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3101 3101 Size: 5830 BtgI C'CryG_G Cuts at: 513 1065 2115 2899 2937 513 Size: 552 1050 784 38 3406 Fragments arranged by size: 3406 1050 784 552 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 3346 4140 4160 3346 Size: 794 20 5016 Fragments arranged by size: 5016 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DraIII CAC_nnn'GTG Cuts at: 2054 2054 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2126 4837 809 Size: 507 810 2711 1802 Fragments arranged by size: 2711 1802 810 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2529 2618 3277 Size: 54 1425 50 275 18 699 89 659 Cuts at: 3277 4166 5795 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 699 659 275 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2669 3760 1353 Size: 1316 1091 3423 Fragments arranged by size: 3423 1316 1091 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2606 4417 4791 4937 387 Size: 1064 138 1017 1811 374 146 1280 Fragments arranged by size: 1811 1280 1064 1017 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2435 2564 2600 3062 3893 4490 5277 Size: 393 803 129 36 462 831 597 787 Cuts at: 5277 5545 1239 Size: 268 1524 Fragments arranged by size: 1524 831 803 787 597 462 393 268 129 36 EcoO109I rG'GnC_Cy Cuts at: 2170 3007 3096 2170 Size: 837 89 4904 Fragments arranged by size: 4904 837 89 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 2725 3036 4977 1678 Size: 1047 311 1941 2531 Fragments arranged by size: 2531 1941 1047 311 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373 Size: 32 135 32 2899 32 42 32 2626 Fragments arranged by size: 2899 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2956 3174 3420 669 Size: 988 281 1018 218 246 3079 Fragments arranged by size: 3079 1018 988 281 246 218 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KasI G'GCGC_C Cuts at: 2721 2721 Size: 5830 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3030 4944 5128 1305 Size: 1725 1914 184 2007 Fragments arranged by size: 2007 1914 1725 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2531 2620 10 Size: 54 1425 343 699 89 3220 Fragments arranged by size: 3220 1425 699 343 89 54 MslI CAynn'nnrTG Cuts at: 518 1166 2312 2375 3788 4147 4306 518 Size: 648 1146 63 1413 359 159 2042 Fragments arranged by size: 2042 1413 1146 648 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2293 3246 3923 5071 5316 1606 Size: 687 953 677 1148 245 2120 Fragments arranged by size: 2120 1148 953 687 677 245 NarI GG'CG_CC Cuts at: 2722 2722 Size: 5830 NcoI C'CATG_G Cuts at: 513 1065 2937 513 Size: 552 1872 3406 Fragments arranged by size: 3406 1872 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 2374 2852 3188 4733 1132 Size: 97 1145 478 336 1545 2229 Fragments arranged by size: 2229 1545 1145 478 336 97 PasI CC'CwG_GG Cuts at: 1348 2074 1348 Size: 726 5104 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5830 PfoI T'CCnGG_A Cuts at: 2541 2541 Size: 5830 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2170 3096 2170 Size: 926 4904 PshAI GACnn'nnGTC Cuts at: 1613 2300 1613 Size: 687 5143 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PstI C_TGCA'G Cuts at: 830 2730 3182 830 Size: 1900 452 3478 Fragments arranged by size: 3478 1900 452 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SanDI GG'GwC_CC Cuts at: 3096 3096 Size: 5830 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 2672 4078 5790 2672 Size: 1406 1712 2712 Fragments arranged by size: 2712 1712 1406 SfcI C'TryA_G Cuts at: 826 1045 2146 2726 2761 3178 4313 4994 5185 Size: 219 1101 580 35 417 1135 681 191 Cuts at: 5185 826 Size: 1471 Fragments arranged by size: 1471 1135 1101 681 580 417 219 191 35 SfoI GGC'GCC Cuts at: 2723 2723 Size: 5830 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 3157 1872 Size: 1285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3934 4835 5097 820 Size: 197 926 1991 901 262 1553 Fragments arranged by size: 1991 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 2471 1517 Size: 954 4876 StyI C'CwwG_G Cuts at: 513 1065 2571 2937 3269 513 Size: 552 1506 366 332 3074 Fragments arranged by size: 3074 1506 552 366 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3857 3874 4027 4212 4657 325 Size: 1255 2277 17 153 185 445 1498 Fragments arranged by size: 2277 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2670 3073 4076 Size: 274 80 33 51 1443 693 403 1003 Cuts at: 4076 5788 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1003 693 403 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5790 5822 5790 Size: 32 5798 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2539 2539 Size: 5830 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 3155 1870 Size: 1285 4545 XmnI GAAnn'nnTTC Cuts at: 2552 3959 2552 Size: 1407 4423 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI