Restriction map for
pFN21AB9846
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AccI AccIII AflII AflIII
AgeI AhdI AocI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspA1I MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI Tru9I Tth111I TthHB8I
Van91I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 268 GACGTC GACGT^C
AclI 848 AACGTT AA^CGTT
AcsI 729 GAATTC [GA]^AATT[CT]
AfaI 1021 GTAC GT^AC
Alw44I 427 GTGCAC G^TGCAC
AlwI 24 GGATC GGATC(4/5)
ApaI 302 GGGCCC GGGCC^C
ApaLI 427 GTGCAC G^TGCAC
ApoI 729 GAATTC [GA]^AATT[CT]
AspHI 427 GTGCAC G[AT]GC[AT]^C
BanII 302 GGGCCC G[GA]GC[CT]^C
BbsI 489 GAAGAC GAAGAC(2/6)
BcgI 18 GCACCTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 489 GAAGAC GAAGAC(2/6)
BsiHKAI 427 GTGCAC G[AT]GC[AT]^C
BspMI 119 ACCTGC ACCTGC(4/8)
BsrBI 175 GAGCGG CCGCTC(-3/-3)
BsrFI 413 GCCGGC [GA]^CCGG[CT]
BssHII 112 GCGCGC G^CGCGC
BstAPI 429 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 413 GCCGGC [GA]^CCGG[CT]
DpnII 25 GATC ^GATC
DraIII 328 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 939 CCTCTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 729 GAATTC G^AATTC
HincII 845 GTCAAC GT[CT]^[GA]AC
HindII 845 GTCAAC GT[CT]^[GA]AC
MboI 25 GATC ^GATC
NaeI 413 GCCGGC GCC^GGC
NdeII 25 GATC ^GATC
NgoMIV 413 GCCGGC G^CCGGC
NspI 431 ACATGC [GA]CATG^[CT]
PpuMI 77 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 838 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 848 AACGTT AA^CGTT
RsaI 1021 GTAC GT^AC
SapI 1057 GCTCTTC GCTCTTC(1/4)
Sau3AI 25 GATC ^GATC
SgrAI 412 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 354 CCCGGG CCC^GGG
SmlI 51 CTCAAG C^T[CT][GA]AG
StuI 1107 AGGCCT AGG^CCT
TfiI 4 GAATC G^A[AT]TC
Tsp509I 730 AATT ^AATT
XmaCI 354 CCCGGG C^CCGGG
XmaI 354 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 113 CGCG CG^CG
757 CGCG
AlwNI 16 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
69 CAGCACCTG
Aor51HI 581 AGCGCT AGC^GCT
804 AGCGCT
Asp700I 4 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
594 GAACAACTTC
AvaI 307 CTCGGG C^[CT]CG[GA]G
354 CCCGGG
AvaII 78 GGACC G^G[AT]CC
223 GGTCC
BfaI 709 CTAG C^TAG
791 CTAG
BseRI 376 GAGGAG GAGGAG(10/8)
913 GAGGAG
BsgI 184 CTGCAC GTGCAG(16/14)
334 GTGCAG
BsoBI 307 CTCGGG C^[CT]CG[GA]G
354 CCCGGG
BstUI 113 CGCG CG^CG
757 CGCG
EarI 1058 CTCTTC CTCTTC(1/4)
658 GAAGAG
Eco47III 581 AGCGCT AGC^GCT
804 AGCGCT
HphI 609 GGTGA GGTGA(8/7)
685 TCACC
Ksp632I 1058 CTCTTC CTCTTC(1/4)
658 GAAGAG
MaeI 709 CTAG C^TAG
791 CTAG
MvnI 113 CGCG CG^CG
757 CGCG
NlaIII 151 CATG CATG^
432 CATG
PleI 478 GACTC GAGTC(4/5)
892 GAGTC
PstI 13 CTGCAG CTGCA^G
520 CTGCAG
SexAI 329 ACCTGGT A^CC[AT]GGT
1023 ACCTGGT
XcmI 132 CCATCGGCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
678 CCACAAATCACCTGG
XmnI 4 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
594 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcyI AluI BbvI BcnI
BmyI BpmI BsaHI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI MaeII
MaeIII MboII MnlI MspI MvaI
MwoI NciI NlaIV Sau96I ScrFI
SfaNI SfcI TseI Tsp45I TspRI