Restriction map for
pFN21AB9846
(Linear) (Six-base) MAPSORT of: KIBB9846.seq Check: 4966 from: 1 to: 1122
KIBB9846.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AatII G_ACGT'C
Cuts at: 0 272 1122
Size: 272 850
AclI AA'CG_TT
Cuts at: 0 849 1122
Size: 849 273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 850 1122
Size: 625 225 272
Fragments arranged by size:
625 272 225
AfeI AGC'GCT
Cuts at: 0 583 806 1122
Size: 583 223 316
Fragments arranged by size:
583 316 223
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 659 691 1122
Size: 659 32 431
Fragments arranged by size:
659 431 32
AlwNI CAG_nnn'CTG
Cuts at: 0 21 74 1122
Size: 21 53 1048
Fragments arranged by size:
1048 53 21
ApaI G_GGCC'C
Cuts at: 0 306 1122
Size: 306 816
ApaLI G'TGCA_C
Cuts at: 0 427 1122
Size: 427 695
ApoI r'AATT_y
Cuts at: 0 729 1122
Size: 729 393
AvaI C'yCGr_G
Cuts at: 0 307 354 1122
Size: 307 47 768
Fragments arranged by size:
768 307 47
BanII G_rGCy'C
Cuts at: 0 306 1122
Size: 306 816
BbsI GAAGACnn'nnnn_
Cuts at: 0 496 1122
Size: 496 626
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 7 41 1122
Size: 7 34 1081
Fragments arranged by size:
1081 34 7
Bme1580I G_kGCm'C
Cuts at: 0 306 431 908 1122
Size: 306 125 477 214
Fragments arranged by size:
477 306 214 125
BmrI ACTGGGnnnn_n'
Cuts at: 0 849 1122
Size: 849 273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 313 454 532 710 1006 1122
Size: 193 120 141 78 178 296 116
Fragments arranged by size:
296 193 178 141 120 116 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 1122
Size: 36 1086
BsaI GGTCTCn'nnnn_
Cuts at: 0 54 982 1102 1122
Size: 54 928 120 20
Fragments arranged by size:
928 120 54 20
BsaHI Gr'CG_yC
Cuts at: 0 269 359 368 1122
Size: 269 90 9 754
Fragments arranged by size:
754 269 90 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 928 1122
Size: 391 537 194
Fragments arranged by size:
537 391 194
BseYI C'CCAG_C
Cuts at: 0 765 801 1122
Size: 765 36 321
Fragments arranged by size:
765 321 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 355 1122
Size: 169 186 767
Fragments arranged by size:
767 186 169
BsiHKAI G_wGCw'C
Cuts at: 0 431 1122
Size: 431 691
Bsp1286I G_dGCh'C
Cuts at: 0 306 431 908 1122
Size: 306 125 477 214
Fragments arranged by size:
477 306 214 125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 1122
Size: 128 994
BsrBI CCG'CTC
Cuts at: 0 177 1122
Size: 177 945
BsrFI r'CCGG_y
Cuts at: 0 413 1122
Size: 413 709
BssHII G'CGCG_C
Cuts at: 0 112 1122
Size: 112 1010
BstAPI GCAn_nnn'nTGC
Cuts at: 0 435 1122
Size: 435 687
BtsI GCAGTG_nn'
Cuts at: 0 112 1122
Size: 112 1010
DraIII CAC_nnn'GTG
Cuts at: 0 333 1122
Size: 333 789
EarI CTCTTCn'nnn_
Cuts at: 0 653 1064 1122
Size: 653 411 58
Fragments arranged by size:
653 411 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 514 1122
Size: 514 608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 313 454 532 625 710 850 1006
Size: 193 120 141 78 93 85 140 156
Cuts at: 1006 1122
Size: 116
Fragments arranged by size:
193 156 141 140 120 116 93 85
78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 943 1122
Size: 943 179
EcoO109I rG'GnC_Cy
Cuts at: 0 78 252 303 948 1122
Size: 78 174 51 645 174
Fragments arranged by size:
645 174 174 78 51
EcoRI G'AATT_C
Cuts at: 0 729 1122
Size: 729 393
HaeII r_GCGC'y
Cuts at: 0 141 585 625 808 1122
Size: 141 444 40 183 314
Fragments arranged by size:
444 314 183 141 40
HincII GTy'rAC
Cuts at: 0 847 1122
Size: 847 275
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 864 1122
Size: 644 220 258
Fragments arranged by size:
644 258 220
NaeI GCC'GGC
Cuts at: 0 415 1122
Size: 415 707
NgoMIV G'CCGG_C
Cuts at: 0 413 1122
Size: 413 709
NspI r_CATG'y
Cuts at: 0 435 1122
Size: 435 687
PasI CC'CwG_GG
Cuts at: 0 908 1122
Size: 908 214
PfoI T'CCnGG_A
Cuts at: 0 231 362 470 1122
Size: 231 131 108 652
Fragments arranged by size:
652 231 131 108
PpuMI rG'GwC_Cy
Cuts at: 0 78 1122
Size: 78 1044
PshAI GACnn'nnGTC
Cuts at: 0 842 1122
Size: 842 280
PspOMI G'GGCC_C
Cuts at: 0 302 1122
Size: 302 820
PstI C_TGCA'G
Cuts at: 0 17 524 1122
Size: 17 507 598
Fragments arranged by size:
598 507 17
SanDI GG'GwC_CC
Cuts at: 0 78 1122
Size: 78 1044
SapI GCTCTTCn'nnn_
Cuts at: 0 1064 1122
Size: 1064 58
SexAI A'CCwGG_T
Cuts at: 0 329 1023 1122
Size: 329 694 99
Fragments arranged by size:
694 329 99
SfcI C'TryA_G
Cuts at: 0 13 450 520 1122
Size: 13 437 70 602
Fragments arranged by size:
602 437 70 13
SgrAI Cr'CCGG_yG
Cuts at: 0 413 1122
Size: 413 709
SmaI CCC'GGG
Cuts at: 0 356 1122
Size: 356 766
SmlI C'TyrA_G
Cuts at: 0 51 1122
Size: 51 1071
SrfI GCCC'GGGC
Cuts at: 0 356 1122
Size: 356 766
StuI AGG'CCT
Cuts at: 0 1109 1122
Size: 1109 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 815 1122
Size: 247 568 307
Fragments arranged by size:
568 307 247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 139 685 1122
Size: 139 546 437
Fragments arranged by size:
546 437 139
XmaI C'CCGG_G
Cuts at: 0 354 1122
Size: 354 768
XmnI GAAnn'nnTTC
Cuts at: 0 8 598 1122
Size: 8 590 524
Fragments arranged by size:
590 524 8
ZraI GAC'GTC
Cuts at: 0 270 1122
Size: 270 852
Enzymes that do cut:
AatII AclI AcuI AfeI AloI AlwNI ApaI ApaLI
ApoI AvaI BanII BbsI BcgI Bme1580I BmrI BpmI
BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BssHII BstAPI BtsI DraIII EarI
EciI Eco57MI EcoNI EcoO109I EcoRI HaeII HincII MmeI
NaeI NgoMIV NspI PasI PfoI PpuMI PshAI PspOMI
PstI SanDI SapI SexAI SfcI SgrAI SmaI SmlI
SrfI StuI TaqII XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AflII AflIII AgeI AhdI AleI
AlfI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DrdI EaeI EagI EcoICRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KasI KpnI MfeI MluI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SbfI ScaI SfiI
SfoI SgfI SnaBI SpeI SphI SspI StyI SwaI
TatI TstI Tth111I XbaI XhoI