Restriction map for
pFN21AB9843
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbvCI BcgI
BciVI BfrI BglI BlnI BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI Ksp632I KspI MfeI MluI
MroI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AlwNI 377 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 888 ATTAAT AT^TAAT
AsnI 888 ATTAAT AT^TAAT
Asp700I 1065 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 324 GTGCTC G[AT]GC[AT]^C
BalI 678 TGGCCA TGG^CCA
BanI 485 GGCACC G^G[CT][GA]CC
BbrPI 321 CACGTG CAC^GTG
BbsI 1054 GAAGAC GAAGAC(2/6)
BclI 21 TGATCA T^GATCA
BcnI 152 CCCGG CC^[GC]GG
BglII 845 AGATCT A^GATCT
BmyI 324 GTGCTC G[AGT]GC[ACT]^C
BpmI 480 CTGGAG CTGGAG(16/14)
BpuAI 1054 GAAGAC GAAGAC(2/6)
BsaAI 321 CACGTG [CT]AC^GT[GA]
BsiHKAI 324 GTGCTC G[AT]GC[AT]^C
BsiYI 395 CCAGGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 395 CCAGGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 78 GTCCC GGGAC(10/14)
Bsp1286I 324 GTGCTC G[AGT]GC[ACT]^C
BspMI 313 ACCTGC ACCTGC(4/8)
BsrI 579 ACTGG ACTGG(1/-1)
BstYI 845 AGATCT [GA]^GATC[CT]
EaeI 678 TGGCCA [CT]^GGCC[GA]
EcoRI 811 GAATTC G^AATTC
EcoT14I 489 CCAAGG C^C[AT][AT]GG
EcoT22I 513 ATGCAT ATGCA^T
FbaI 21 TGATCA T^GATCA
HaeIII 679 GGCC GG^CC
HapII 153 CCGG C^CGG
HincII 304 GTTGAC GT[CT]^[GA]AC
HindII 304 GTTGAC GT[CT]^[GA]AC
HindIII 1078 AAGCTT A^AGCTT
HinfI 405 GACTC G^A[ACGT]TC
HpaII 153 CCGG C^CGG
MaeII 322 ACGT A^CGT
MflI 845 AGATCT [GA]^GATC[CT]
MluNI 678 TGGCCA TGG^CCA
MscI 678 TGGCCA TGG^CCA
MspI 153 CCGG C^CGG
MwoI 317 GCTGCACGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 152 CCCGG CC^[GC]GG
NlaIV 485 GGCACC GG[ACGT]^[ACGT]CC
NsiI 513 ATGCAT ATGCA^T
NspI 414 GCATGC [GA]CATG^[CT]
PleI 405 GACTC GAGTC(4/5)
PmaCI 321 CACGTG CAC^GTG
PmlI 321 CACGTG CAC^GTG
PshBI 888 ATTAAT AT^TAAT
SfcI 347 CTATAG C^T[GA][CT]AG
SphI 414 GCATGC GCATG^C
StyI 489 CCAAGG C^C[AT][AT]GG
XhoII 845 AGATCT [GA]^GATC[CT]
XmnI 1065 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 736 GTAC GT^AC
854 GTAC
AlwI 594 GATCC GGATC(4/5)
357 GGATC
BfaI 497 CTAG C^TAG
739 CTAG
BsaBI 358 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
588 GATTTTGATC
BseRI 470 CTCCTC GAGGAG(10/8)
667 GAGGAG
BsgI 160 CTGCAC GTGCAG(16/14)
318 CTGCAC
BsmAI 642 GAGAC GTCTC(1/5)
800 GTCTC
MaeI 497 CTAG C^TAG
739 CTAG
MamI 358 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
588 GATTTTGATC
MboII 976 GAAGA GAAGA(8/7)
1054 GAAGA
RsaI 736 GTAC GT^AC
854 GTAC
TaqI 366 TCGA T^CGA
1092 TCGA
TthHB8I 366 TCGA T^CGA
1092 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsaJI
BsmI BssKI BstF5I BstNI BstXI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HphI ItaI MaeIII MboI
MnlI MseI MvaI NdeII NlaIII
Sau3AI ScrFI SfaNI Tru9I TseI
Tsp45I Tsp509I TspRI