(Circular) (Six-base) MAPSORT of: pFN21AB9843.seq Check: 9272 from: 1 to: 5803 pFN21AB9843 5803 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3613 278 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3146 1937 Size: 1209 4594 Acc65I G'GTAC_C Cuts at: 3124 3124 Size: 5803 AclI AA'CG_TT Cuts at: 1563 3930 4303 1563 Size: 2367 373 3063 Fragments arranged by size: 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2224 3000 3866 5250 5518 1632 Size: 592 776 866 1384 268 1917 Fragments arranged by size: 1917 1384 866 776 592 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5606 AflIII A'CryG_T Cuts at: 1203 4702 5679 1203 Size: 3499 977 1327 Fragments arranged by size: 3499 1327 977 AgeI A'CCGG_T Cuts at: 4634 4634 Size: 5803 AhdI GACnn_n'nnGTC Cuts at: 4532 4532 Size: 5803 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5803 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5803 AlwNI CAG_nnn'CTG Cuts at: 2387 3546 5118 2387 Size: 1159 1572 3072 Fragments arranged by size: 3072 1572 1159 ApaLI G'TGCA_C Cuts at: 3860 5016 3860 Size: 1156 4647 ApoI r'AATT_y Cuts at: 1493 2017 2073 2784 2816 2973 3112 3338 3490 Size: 524 56 711 32 157 139 226 152 Cuts at: 3490 5599 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 711 524 226 157 152 139 56 32 AseI AT'TA_AT Cuts at: 160 1032 2894 4357 160 Size: 872 1862 1463 1606 Fragments arranged by size: 1862 1606 1463 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 AvaI C'yCGr_G Cuts at: 1870 1943 3128 1870 Size: 73 1185 4545 Fragments arranged by size: 4545 1185 73 BamHI G'GATC_C Cuts at: 3133 3500 3133 Size: 367 5436 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2490 3124 4579 618 Size: 325 217 627 703 634 1455 1842 Fragments arranged by size: 1842 1455 703 634 627 325 217 BanII G_rGCy'C Cuts at: 1391 3122 1391 Size: 1731 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3066 928 Size: 971 1167 3665 Fragments arranged by size: 3665 1167 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3994 4028 1903 Size: 34 2057 34 3678 Fragments arranged by size: 3678 2057 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3695 4905 3695 Size: 1210 4593 BclI T'GATC_A Cuts at: 1548 2026 1548 Size: 478 5325 BfrBI ATG'CAT Cuts at: 2520 2520 Size: 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4414 136 Size: 107 122 71 631 605 2742 1525 Fragments arranged by size: 2742 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2850 5798 2850 Size: 2948 2855 BlpI GC'TnA_GC Cuts at: 3220 3220 Size: 5803 Bme1580I G_kGCm'C Cuts at: 1790 3864 5020 1790 Size: 2074 1156 2573 Fragments arranged by size: 2573 2074 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5803 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3534 4492 442 Size: 416 53 83 399 24 2117 958 1753 Fragments arranged by size: 2117 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2506 4463 1239 Size: 1267 1957 2579 Fragments arranged by size: 2579 1957 1267 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3928 4793 5091 3928 Size: 865 298 4640 Fragments arranged by size: 4640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4466 882 Size: 617 2967 2219 Fragments arranged by size: 2967 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2328 3594 493 Size: 711 1124 1266 2702 Fragments arranged by size: 2702 1266 1124 711 BsaBI GATnn'nnATC Cuts at: 2367 2597 5797 2367 Size: 230 3200 2373 Fragments arranged by size: 3200 2373 230 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3610 3992 4627 275 Size: 53 83 186 936 2077 382 635 1451 Fragments arranged by size: 2077 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4236 4634 4908 5055 5466 4236 Size: 398 274 147 411 4573 Fragments arranged by size: 4573 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5578 Fragments arranged by size: 5578 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2466 2687 1186 Size: 1280 221 4302 Fragments arranged by size: 4302 1280 221 BseYI C'CCAG_C Cuts at: 5006 5502 5006 Size: 496 5307 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2308 1163 Size: 573 414 158 4658 Fragments arranged by size: 4658 573 414 158 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3253 4014 5042 1540 Size: 275 186 1252 761 1028 2301 Fragments arranged by size: 2301 1252 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2333 3864 3949 5020 2333 Size: 1531 85 1071 3116 Fragments arranged by size: 3116 1531 1071 85 BsmI GAATG_Cn' Cuts at: 2067 2522 3074 3314 3407 2067 Size: 455 552 240 93 4463 Fragments arranged by size: 4463 552 455 240 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2333 3122 3864 3949 5020 1391 Size: 399 543 789 742 85 1071 2174 Fragments arranged by size: 2174 1071 789 742 543 399 85 BspEI T'CCGG_A Cuts at: 5466 5466 Size: 5803 BspHI T'CATG_A Cuts at: 3692 5422 3692 Size: 1730 4073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2327 3158 844 Size: 1483 831 3489 Fragments arranged by size: 3489 1483 831 BsrBI CCG'CTC Cuts at: 1394 1823 3690 1394 Size: 429 1867 3507 Fragments arranged by size: 3507 1867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4298 4472 65 Size: 1183 3050 174 1396 Fragments arranged by size: 3050 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4447 4634 1700 Size: 110 2637 187 2869 Fragments arranged by size: 2869 2637 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5803 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5803 BssSI C'ACGA_G Cuts at: 3863 4875 3863 Size: 1012 4791 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5803 BstBI TT'CG_AA Cuts at: 4607 4607 Size: 5803 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5803 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2887 2998 2077 Size: 810 111 4882 Fragments arranged by size: 4882 810 111 BstYI r'GATC_y Cuts at: 1972 2850 3133 3500 3885 3902 5354 5440 5452 Size: 878 283 367 385 17 1452 86 12 Cuts at: 5452 5798 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 878 385 367 346 283 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4312 Fragments arranged by size: 4312 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3106 487 Size: 683 56 1880 3184 Fragments arranged by size: 3184 1880 683 56 BtsI GCAGTG_nn' Cuts at: 3319 4113 4133 3319 Size: 794 20 4989 Fragments arranged by size: 4989 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2571 2606 529 Size: 35 2007 35 3726 Fragments arranged by size: 3726 2007 35 35 DraI TTT'AAA Cuts at: 3107 3107 Size: 5803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4810 809 Size: 507 3494 1802 Fragments arranged by size: 3494 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2683 3250 4139 Size: 54 1425 50 275 18 853 567 889 Cuts at: 4139 5768 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 853 567 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3250 1537 Size: 275 1438 4090 Fragments arranged by size: 4090 1438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3733 1353 Size: 2380 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4390 4764 4910 387 Size: 1064 138 2801 374 146 1280 Fragments arranged by size: 2801 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2224 2506 3000 3866 4463 5250 5518 Size: 393 592 282 494 866 597 787 268 Cuts at: 5518 1239 Size: 1524 Fragments arranged by size: 1524 866 787 597 592 494 393 282 268 EcoRI G'AATT_C Cuts at: 2816 3112 3490 2816 Size: 296 378 5129 Fragments arranged by size: 5129 378 296 FspI TGC'GCA Cuts at: 4309 4309 Size: 5803 HaeII r_GCGC'y Cuts at: 1678 4950 1678 Size: 3272 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4444 4476 4518 4550 1373 Size: 32 135 32 2872 32 42 32 2626 Fragments arranged by size: 2872 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2311 3147 3393 669 Size: 988 281 373 836 246 3079 Fragments arranged by size: 3079 988 836 373 281 246 HindIII A'AGCT_T Cuts at: 748 3083 748 Size: 2335 3468 HpaI GTT'AAC Cuts at: 3393 3393 Size: 5803 KpnI G_GTAC'C Cuts at: 3128 3128 Size: 5803 MfeI C'AATT_G Cuts at: 3402 3402 Size: 5803 MluI A'CGCG_T Cuts at: 5679 5679 Size: 5803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4917 5101 1305 Size: 3612 184 2007 Fragments arranged by size: 3612 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2685 10 Size: 54 1425 343 853 3128 Fragments arranged by size: 3128 1425 853 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3761 4120 4279 518 Size: 648 2595 359 159 2042 Fragments arranged by size: 2595 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3219 3896 5044 5289 1606 Size: 1613 677 1148 245 2120 Fragments arranged by size: 2120 1613 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4312 Fragments arranged by size: 4312 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5803 NotI GC'GGCC_GC Cuts at: 3250 3250 Size: 5803 NsiI A_TGCA'T Cuts at: 2522 2522 Size: 5803 NspI r_CATG'y Cuts at: 1132 1229 2423 3161 4706 1132 Size: 97 1194 738 1545 2229 Fragments arranged by size: 2229 1545 1194 738 97 PasI CC'CwG_GG Cuts at: 1348 2887 1348 Size: 1539 4264 PciI A'CATG_T Cuts at: 4702 4702 Size: 5803 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5803 PmeI GTTT'AAAC Cuts at: 3107 3107 Size: 5803 PmlI CAC'GTG Cuts at: 2328 2328 Size: 5803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3872 3904 5385 5417 3872 Size: 32 1481 32 4258 Fragments arranged by size: 4258 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5803 PsiI TTA'TAA Cuts at: 3029 3373 3029 Size: 344 5459 PstI C_TGCA'G Cuts at: 830 3155 830 Size: 2325 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5803 SalI G'TCGA_C Cuts at: 1936 3145 1936 Size: 1209 4594 SbfI CC_TGCA'GG Cuts at: 3155 3155 Size: 5803 ScaI AGT'ACT Cuts at: 4051 5763 4051 Size: 1712 4091 SfcI C'TryA_G Cuts at: 826 1045 2352 3151 4286 4967 5158 826 Size: 219 1307 799 1135 681 191 1471 Fragments arranged by size: 1471 1307 1135 799 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5803 SmaI CCC'GGG Cuts at: 1872 3130 1872 Size: 1258 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3907 4808 5070 820 Size: 197 926 1964 901 262 1553 Fragments arranged by size: 1964 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3594 493 Size: 3101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5803 SphI G_CATG'C Cuts at: 1132 2423 3161 1132 Size: 1291 738 3774 Fragments arranged by size: 3774 1291 738 SspI AAT'ATT Cuts at: 5 52 3727 5 Size: 47 3675 2081 Fragments arranged by size: 3675 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5803 StyI C'CwwG_G Cuts at: 513 1065 2004 2494 3242 513 Size: 552 939 490 748 3074 Fragments arranged by size: 3074 939 748 552 490 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2891 3830 3847 4000 4185 4630 325 Size: 1255 1311 939 17 153 185 445 1498 Fragments arranged by size: 1498 1311 1255 939 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2740 4049 5761 Size: 274 80 33 51 1443 763 1309 1712 Cuts at: 5761 96 Size: 138 Fragments arranged by size: 1712 1443 1309 763 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2528 2560 5763 5795 2528 Size: 32 3203 32 2536 Fragments arranged by size: 3203 2536 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5803 XbaI T'CTAG_A Cuts at: 3139 3139 Size: 5803 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5803 XmaI C'CCGG_G Cuts at: 1870 3128 1870 Size: 1258 4545 XmnI GAAnn'nnTTC Cuts at: 3074 3932 3074 Size: 858 4945 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3611 276 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI